Objective To establish an HPLC method for the determination of schisanhenol in plasma and to study the pharmacokinetics of schisanhenol in rats.Methods After sedimentation by methanol,plasma samples were then prepared based on a liquid-liquid extraction by ether.The extracted samples were analyzed by liquid chromatography.Schisanhenol was eluted on Eclipse XDB-C18 Agilent(250 mm?4.6 mm,5 ?m) column,using a mobile phase of acetonitrile-H2O(65∶35),and detected at 254 nm.The plasma concentration of schisanhenol in rats was determined after iv administration of 18 mg/kg,and the data were processed with the pharmacokinetic software 3P87.Results Calibration curves were linear over 0.1—2.5 ?g/mL (r2=0.999) and the LOD was 10 ng/mL.The recoveries of schisanhenol from plasma were between 88%—110%,and the RSD values of intra-day and interday assay were below 15%.After iv administration at 18 mg/kg,the schisanhenol concentration-time curve confirmed in a two-compartment model and the pharmacokinetic parameters of t1/2?,t1/2?,V,AUC,MRT were(0.22?0.11) h,(1.19?0.22) h,(12.81?2.91) L/kg,(1.32?0.19) ?g/mL/h,(1.51?0.24) h,respectively.Conclusion A reliable HPLC-DAD method is developed for the determination of schisanhenol in rat plasma and it is applicable to the in vivo analysis.

Objective:The aim of this study was to examine whether PSA levels were affected by BMI and the influence of BMI on the clinical application of PSA.Methods:A total of 856 males who had routine health checkups between January 2006 and July 2007 in our hospital were recruited for this study.Those with a documented history of prostate carcinoma were excluded.Height,weight, and total PSA were measured.The BMl was calculated from measured height and weight [BMl=weight(kg)/height(m)2].Results:The serum PSA(ng/mL)levels were 2.97±0.15 in the normal weight group,2.53±0.12 in the overweight group,and 2.01±0.16 in the obese group(P＜0.05).A negative correlation was found between PSA and BMI(r=-0.130,P＜0.05)and weight(r=-0.142.P＜0.05).A positive correlation was found between PSA and age(r=0.306,P＜0.05).Con-clusion:BMl is associated with PSA.As BMI increases, serum PSA decreases.The current data suggest that such an influence should be considered in the clinical application of PSA.

Objective To investigate the effect of ulinastatin (UTI) on hepatic ischemia-reperfusion injury in rats. Methods Totally 24 adult Sprague-Dawley rats were randomly divided into 3 groups: sham-operated control group (SO group), ischemia-reperfusion group (I/R group) and ulinastatin group (UTI group). Liver in I/R group underwent 1 h of reperfasion after 30 min of ischemia. In UTI group, UTI (2×104 U/kg) was administered to rats 30 min before modeling. The levels of alanine aminotransferase, aspartate aminotransferase and tumor necrosis factor-alpha (TNF-α) in serum were measured and the levels of nitric oxide and malondialdehyde in liver were determined. The histological changes of liver were observed. Results The levels of alanine aminotransferase, aspartate aminotransferase and TNF-α in serum were significantly increased in I/R group compared with those in UTI group (P<0.05). The levels of nitric oxide and malondialdehyde in liver were significantly higher in I/R group than in UTI group (P<0.05).Histological examination of liver indicated that the damages were more severe in I/R group than in UTI group.Conclusion UTI has the ability to inhibit the production of TNF-α and oxyradical, and ameliorate microcirculatory dysfunction in rats with hepatic ischemia-reperfasion injury.

Objective: To observe effect of square dance exercises on symptom scores in menopausal women with coronary heart disease (CHD). Methods: A total of 39 menopausal CHD patients participated in square dance exercises, exercise intensity was 47.2%HRmax～58.1%HRmax（60~120 min/d）, 5~7 times/week, three months, and they were regard as dance group. Control group consisted of 42 CHD women of same age living in the same district, who didn’t take part in exercise. After enrollment, these two groups received assessments of Seattle angina questionnaire (SAQ) and frequent physical and mental symptoms of menopausal women self-rating scale (Kupperman self-rating scale) within 3d and after three months. Results: There were no significant difference in score of each item and total score of SAQ in first assessment between two groups (P>0.05 all). Compared with first assessment and control group after three months, there were significant increase in scores of most items and total score of SAQ [(66.40±15.16) scores vs. (67.18±13.93) scores vs. (85.15±16.30) scores] in dance exercises group, P<0.01~0.05. There were no significant difference in score of each item and total score of Kupperman scale in first assessment between two groups (P>0.05 all). Compared with first assessment and control group after three months, there were significant decrease in scores of most items and total score of Kupperman scale [(29.40±6.51) scores vs. (29.73±6.26) scores vs. (23.65±6.29) scores] in dance exercises group, P<0.01~0.05. Conclusion: Square dance exercises may significantly decrease angina pectoris symptom and menopausal physical and mental symptoms in menopausal women with coronary heart disease.

Medical rescue is the most important key point for deciding disaster prognosis. But there are not systemic and special mode of disaster medical education. This article expounds the concept and features of disaster medicine, and puts forward the idea that the training routes of talents in disaster medicine should be included in undergraduates, vocational and continuing education.

ObjectiveTo investigate the changes of serum growth hormone (GH)/insulin-like growth factor- Ⅰ ( IGF- Ⅰ ) in patients with traumatic long bone fractures and the related clinical significance.MethodsTwenty-one patients with traumatic long bone fractures from the same trauma care center treated between November 2009 and April 2010 were enrolled in the study..There were 14 males and 7 females,at age range of 22-60 years (mean 45.0 years).Seventeen patients (81％) were with closed trauma and four ( 19％ ) with open trauma.Enzyme Linked Immunoadsorbent Assay (ELISA) was used to determine the levels of serum GH and IGF- Ⅰ within 14 days after fractures and the relationship between changes of GH-IGF- Ⅰ axis and injury severity score (ISS) was further analyzed.Eight patients with normal GH/IGF- Ⅰ were set as control group.ResultsThe GH level in the peripheral blood was significantly higher at day 1 after fractures ( P ＜ 0.05 ),and rapidly declined and stabilized at day 5 compared with the control group.The GH level in the patients with ISS≥ 18 points was sigmficantly higher than that in those with ISS＜18 points at day 1 after fractures (P＜0.05).The IGF- Ⅰ level in the peripheral blood in the patients with 1SS≥ 18 points was significantly higher than that in those with ISS ＜ 18 points at days 5-10 after fractures ( P ＜ 0.05 ).ConclusionThe hypothalamus GH-IGF- Ⅰ axis plays an important role in early metabolism and fracture healing during the acute phase of traumatic long bone fractures.

Objective To investigate effects of combined use of glycogen synthase kinase (GSK) -3 inhibitor and fructose-1,6-diphosphate (FDP) on liver trauma in rats. Methods After crea-tion of liver trauma model in 49 Sprague-Dawley rats, 42 rats were randomly divided into control group (NaCl group), FDP group and FGI Group (FDP and GSK-3 inhibitor in combination group). Then, each group was randomly subdivided into pre-ischemia group and 4-hour reperfusion group on account of time point when animals were sacrificed before and after iachemia. The other seven rats set as sham operation group (SH group) were sacrificed at 4-hour reperfusion time point. The AST and ALT levels in hlood and glycogen content, SOD vitality and MDA content in liver tissues were determined. Results At pre-is-chemia time point, liver glycogen content in three groups was in order of control group < FDP group < FGI group (P <0.01). At 4-hour reperfusion time point, blood ALT and AST levels in four groups were in order of control group > FDP group > FGI group > SH group (P < 0.01), while SOD vitality in liver tissues of four groups was in order of control group < FDP group < FGI group < SH group (P < 0.01) and MDA content in four groups was in order of control group > FDP group > FGI group > SH group (P < 0.01). Conclusions Combined use of FDP and GSK-3 inhibitor can enhance the protective effect of FDP on liver rupture, as may relate to the mechanism that GSK-3 inhibitor can effectively enhance glycogen synthesis of FDP as substrate before liver ischemia so that the liver glycogen storage is increased in a short period of time and hence post-traumatic warm ischemia-reperfusion injury is alleviated in the liver of rats.

By using thrombolysis therapy,34 patients suffering from acute myocardial infarction were treated.And by comparing iron.zinc,copper,manganese and selenium contents in serum of 34 patients suffering from acute myocardial infarction with that of control group and the contents between before-treating and after-treating,following results are revealed:before treating,iron,zinc,copper contents in serum of the patients increased while manganese and selenium contents decreased,there is great difference between the contents of the patients and control group(P

Objective To study the preparation of volatile oils obtained from asarum and lily magnolia complexed with ?-cyclodextrin and hydroxypropyl-?-cyclodextrin in Xuetie Dingchuan plaster,and to study its impacts of transdermal absorption.Methods The volatile oil was complexed with ?-cyclodextrin and hydroxypropyl-?-cyclodextrin.GC method was used in the determination of volatile oils complexed with ?-cyclodextrin and hydroxypropyl-?-cyclodextrin to investigate the impacts of transdermal absorption.Results The accumulation transmission of the volatile oils complexed with ?-cyclodextrin was superior to that with hydroxypropyl-?-cyclodextrin.Conclusion The inclusion of volatile oils with ?-cyclodextrin can enhance drug penetration via skin.

Objective To investigate the relationship between the cerebral blood flow filling condition and prognosis of patients with acute severe cerebral infarction.Methods The clinical data and MR features of 10 cases of acute severe cerebral infarction were retrospectively analyzed.Results The regional cerebral blood flow(rCBF) and regional cerebral blood volume(rCBV) of 9 cases(90%) in the infarcted side decreased significantly.The reduced percents of the infarcted side reached 64.1% and 49.7% respectively.There were significant differences between two opposite sides(P=0.001,P=0.01).The MRA results showed great vessels(arteria carotis interna or arteria cerebri media) in the infarcted side were all obliterated in the all patients,and simultaneous great vessels in opposite side were narrow or occlusion in 7 cases(70%).2 cases died;and 8 cases were serious disability.Conclusions The cerebral blood flow filling of the acute severe cerebral infarction patients decrease significantly.The prognosis of the patients is quite bad.