Objective To investigate the effect of cluster nursing in the prevention of aspiration pneumonia in patients with cerebral infarction. Methods A total of 72 patients with acute cerebral infarction from January 2014 to January 2014 were recruited and divided into 2 groups with 36 cases each by random digits table method. The patients in control group were given diet nursing, nursing of sputum ejection, education of disease related knowledge and another routine nursing methods. While cluster nursing such as the application of swallowing function training, feeding management, homemade angle measuring instrument for position management were conducted on the patients in intervention group. The difference of incidence of aspiration pneumonia, improvement of swallowing function, and patient satisfaction between two groups were compared. Results The incidence of aspiration pneumonia was 5.56%(2/36) in intervention group and 25.00%(9/36) in control group, and the difference was statistically significant (χ2=5.258, P=0.022).The swallowing function ratings after treatment in intervention group was also obviously better than that in control group, and the difference was statistically significant (χ2=7.943, P=0.036). The patient satisfaction was 94.44%(34/36) in intervention group and 63.89%(23/36) in control group, and the difference was statistically significant(χ2=17.526,P=0.000). Conclusions Cluster nursing can effectively reduce the risk of aspiration pneumonia in acute cerebral infarction patients, and help to improve the patients' swallowing function and relationship between nurses and patients, has certain clinical application value.

Mitofusin-2 (Mfn2) gene expression is positively correlated with insulin sensitivity in patients with type 2 diabetes. However, it is unclear if Mfn2 is involved in carbohydrate metabolism and lipid homeostasis. In order to investigate the specific functions of Mfn2 in glycometabolism and lipid homeostasis in BALB/c mice, a RNA interference technique-mediated hydrodynamic injection was developed, in which short hairpin RNAs (shRNAs) were used to inhibit the Mfn2 expression in vivo. Seventy-two mice were randomly divided into two groups: the Mfn2 reduction group (Mfn2/shRNA) and the negative control group (NC). Intraperitoneal glucose tolerance tests and intraperitoneal insulin tolerance tests were used to evaluate glycometabolism and insulin sensitivity. D-(3-(3)H) glucose or (3)H(2)O was injected into the tail vein or intraperitoneally to facilitate the calculation of the rate of hepatic glucose production and fatty acid synthesis in vivo. The results showed that, in Mfn2/shRNA mice, the liver Mfn2 protein was significantly decreased, and fasting blood glucose concentrations were increased by approximately 48%, when compared with the NC mice. In parallel with the changes in fasting glucose levels, hepatic glucose production was significantly elevated in Mfn2/shRNA mice. When insulin was administrated, these mice exhibited impaired insulin tolerance. It was also found that the reduction of Mfn2 markedly decreased the rate of fatty acid synthesis in the liver, and the Mfn2/shRNA mice exhibited hypertriglyceridema. Taken together, our results indicate that Mfn2 plays an important role in maintaining glucose and lipid homeostasis, and in the development of insulin resistance in vivo.

Objective To investigate effects of rosiglitazone on glucose transport-4(GLUT4) protein translocation in skeletal muscle of insulin resistance rats. Methods 38 male sprague dawley rats were randomized to received high fat or standard chow diet for 8 weeks, feeding high-fat diet to induce insulin resistance in skeletal muscle of rats. 25 high-fat-fed rats were randomly treated with rosiglitazone(1mg/kg), standard chow diet, or placebo(high-fat diet) for 4 weeks. Plasma concentrations of glucose and insulin, weight and viscero-fat in all rats were measured. The GLUT4 level in the cell membrane of isolated rats skeletal muscle was detected by western blotting analysis. Results Insulin-stimulated translocation of GLUT4 to the plasma membrane in skeletal muscles of high fat-fed rats was significantly lower than that of standard chow-fed rats(52 72%,P

OBJECTIVE:To observe the effect of compound glycyrrhizin on patients with chronic type B hepatitis.METH_ ODS:126patients with chronic type B hepatitis were randomly divided into two groups:the control group was administered with common therapy like reducing jaundice and protecting liver;the trial group was administered with compound glycyrrhizin plus the therapy of the control group.Time of therapy for both groups was8wk.RESULTS:The total effective rates for the tri_ al group and the control group were93.6%and33.3%,respectively,and significant differences were noted between groups(P

The effects of intravenous injection of purifed EC on the rectal tempera-ture in normal rabbits and febrile rabbits induced by ET and concentrations of Fe~(2+), Zn~(2+),Cu~(2+) in serum were observed. The results showed that EC could lower the rectal tempera-ture in normal rabbits and also the increased rectal temperature in febrile rabbits. Mean-while, EC could inhibit the formation of the second phase of ET-fever. But EC had noeffect on the changed concentrations of FC~(2+), Zn~(2+), Cu~(2+) in serum caused by ET. Thesefindings suggest that EC may particiate in the regulation of body temperarure under physio-logical and febrile conditions, but not of acute phase response produced by ET.

Objective To establish an HPLC method for the determination of schisanhenol in plasma and to study the pharmacokinetics of schisanhenol in rats.Methods After sedimentation by methanol,plasma samples were then prepared based on a liquid-liquid extraction by ether.The extracted samples were analyzed by liquid chromatography.Schisanhenol was eluted on Eclipse XDB-C18 Agilent(250 mm?4.6 mm,5 ?m) column,using a mobile phase of acetonitrile-H2O(65∶35),and detected at 254 nm.The plasma concentration of schisanhenol in rats was determined after iv administration of 18 mg/kg,and the data were processed with the pharmacokinetic software 3P87.Results Calibration curves were linear over 0.1—2.5 ?g/mL (r2=0.999) and the LOD was 10 ng/mL.The recoveries of schisanhenol from plasma were between 88%—110%,and the RSD values of intra-day and interday assay were below 15%.After iv administration at 18 mg/kg,the schisanhenol concentration-time curve confirmed in a two-compartment model and the pharmacokinetic parameters of t1/2?,t1/2?,V,AUC,MRT were(0.22?0.11) h,(1.19?0.22) h,(12.81?2.91) L/kg,(1.32?0.19) ?g/mL/h,(1.51?0.24) h,respectively.Conclusion A reliable HPLC-DAD method is developed for the determination of schisanhenol in rat plasma and it is applicable to the in vivo analysis.

Pedicle screw fixation is biomechanically most reliable for reconstruction of the cervical spine, which can ef?fectively restore cervical sagittal profiles, provide rigid fixation and higher fusion rate. Because of the large individual differ?ences in cervical pedicle, vertebral artery and nerve root, preoperative evaluation should be individualized. Cervical pedicle screw techniques include freehand technique and the computer-assisted navigation technology developed recently. Although complications associated with cervical pedicle screw fixation cannot be completely obviated, they can be minimized by com?bination of sufficient preoperative imaging studies of the pedicles and meticulous surgical techniques of screw placement. Cervical pedicle screw fixation techniques have been used in the treatment of various cervical disorders, with fewer complica?tions and good clinical efficacy, and a wide range of clinical applications.

Objective To develop a virtual endoscopy system which can be integrated into PACS.Methods Key techniques on virtual endoscopy were researched and we implemented a virtual endoscopy system with the help of the Visualization Toolkit VTK.Results The Virtual endoscopy system was integrated into PACS and the post-processing function of PACS was advanced.Conclusion As a novel medical image post-processing technology,virtual endoscopy provides a completely non-invaded inspection,so it has broad application prospects in the computer-aided medical teaching,surgical navigation,surgical planning and clinical diagnosis.

Objective To explore the effect of dexamethasone(Dex)treatment on the expression of interleukin(IL)?6 and IL?8 in human peri?odontal ligament cells(hPDLCs). Methods hPDLCs were subjected to one of the following treatments for 24 h or 48 h:10-9 mol/L Dex;10-6 mol/L Dex;10μg/mL Porphyromonas gingivalis(P. g)?lipopolysaccharide(LPS);10-9 mol/L Dex+10μg/mL P. g?LPS;10-6 mol/L Dex+10μg/mL P. g?LPS;or 0.1%absolute ethyl alcohol(control). Protein and mRNA expression was detected using ELISA and real?time PCR ,respectively. Results At 24 h and at 48 h,IL?6 and IL?8 protein expression in the 10-9 mol/L Dex?and 10-6 mol/L Dex?treated groups was significantly lower than that in the control group(P<0.05). At 48 h,IL?6 mRNA expression in the 10-9 mol/L Dex?and 10-6 mol/L Dex?treated groups was signifi?cantly lower than that in the control group(P<0.05),while IL?8 mRNA expression in the 10-6 mol/L Dex?treated group was significantly higher than that in the control group(P<0.05). At 24 h and at 48 h,IL?6 protein and mRNA expression in the 10-9 mol/L Dex+10μg/mL P. g?LPS?treated group and the 10-6 mol/L Dex+10μg/mL P. g?LPS?treated group was significantly lower than that in the 10μg/mL P. g?LPS?treated group (P<0.05). At 24 h,IL?8 protein expression in the 10-9 mol/L Dex+10μg/mL P. g?LPS?treated group and the 10-6 mol/L Dex+10μg/mL P. g?LPS?treated group was significantly lower than that in the 10μg/mL P. g?LPS?treated group(P<0.05),while no such significant difference exist?ed at 48 h. At 48 h,IL?8 mRNA expression in the 10-6 mol/L Dex+10μg/mL P. g?LPS?treated group was significantly higher than that in the 10μg/mL P. g?LPS?treated group(P<0.05). Conclusion Dex inhibits the innate and P. g?LPS?induced expression of IL?6 in hPDLCs. However, Dex exerts profound effects on IL?8 expression,and treatment with high doses of Dex may promote IL?8 expression over an extended period.

Objective To assess the effect of intraperitoneal interstitial implant of Sustained-release 5-Fluorouracil during operation for advanced rectal cancer. Methods One hundred and four cases with advanced rectal cancer were enrolled into the study and divided into 3 groups: treatment group 1(n = 37), underwent combined hypotonic peritoneal chemo-hyperthermia and Sustain-released 5-fluorouracil implantation treatment during operation; treatment group 2(n = 34), underwent combined intraoperative hypotonic peritoneal chemohyperthermia and early postoperatively intraperitoneal chemotherapy treatment; and control group(n = 33),underwent peritoneal lavage during radical resection alone. Results Digestive tract toxicity in group 1 was significantly lower than that in group 2(2. 70% vs. 20. 59%, x2 = 9. 104, P ＜ 0. 05). There were significant difference on 1 year survival rate among the three groups. The 2-year survival rate of group 1 was significantly higher than that in the control group (78. 37% vs. 48. 48%, x2 = 1. 297 ,P ＜ 0. 05). After two years follow-up the 2-year local recurrence rate of group 1 was significantly lower than that in the control group(21.62% vs 32. 35 %, x2 = 7. 001, P ＜ 0. 05). However, no significant difference was found compared to group 2, which was 32. 35%. Conclusion lntraperitoneal interstitial implant of Sustain-released 5-Fluorouracil during operation is an effective intraperitoneal chemotherapy with mild side effects for advanced rectal cancer.