1.Key and Difficult Points of Nosocomial Infection Management in Department of Stomatology
Xiaolian LU ; Zhizhong WANG ; Guimian ZOU
Chinese Journal of Nosocomiology 2004;0(10):-
OBJECTIVE To explore the key and difficult points of nosocomial infection in department of stomatology and propose some effective measures.METHODS All measures for preventing nosocomial infection in our hospital stomatology center were analyzed.RESULTS The key point of nosocomial infection management was to prevent various infections via blood,saliva and tissue of mouth.Meanwhile there were many difficult points in conception change and in diagnosis and treatment technique of nosocomial infection management.CONCLUSIONS It is necessary to pay more attention to the management of nosocomial infection and some effective management measures must be taken in to prevent nosocomial infection of stomatological department.
2.Effect of estradiol on the gene expression of peroxisome proliferator-activated receptor gamma-2 in rat bone marrow stromal cells
Chengliang YUAN ; Xiaolian JIN ; Shigang WEI ; Xiying ZOU
Chinese Journal of Tissue Engineering Research 2007;11(3):596-600
BACKGROUND: Whehter estradiol (E2) can change the expression of peroxisome proliferator-activated receptor gamma-2(PPAR-γ2) during differentiation of bone marrow stroma cells should be studied further.OBJECTIVE: To investigate the effects of 17β-estradiol (E2) on the gene expression of PPAR-γ2 mRNA and PPAR-γ2 protein.DESIGN: Contrast observational trial.SETTING: Laboratory Department of People's Hospital of Deyang City; Laboratory Center of Chengdu Military Command General Hospital and Chengdu Bai'ao Biol-Tech Limited Company.MATERTALS: A 3-month-old female SD rat (200±20) g used to isolate bone marrow stromal cells was obtained from Animal center of Chengdu Chinese Medicine University (Medical Experimental Animal Number 11). Dulbecco's mimimum essential medium (DMEM) was obtained from Hyctone Compnay. 1α, 25(OH)2D3, dexamethasome (DEX) and E2 were purchased from Sigma Company. Total RNA kits were obtained from Omga. One step RNA PCR kit (AMV) was obtained from Takara Shuzo Co, Ltd. Northern direct HRP labeling and detection kit was purchased from PIERCE. Western blotting luminol reagent was obtained from Santa cruz.METHODS: The experiment was carried out from April 2001 to July 2002 in the Laboratory Department of People's Hospital of Deyang City, Laboratory Center of Chengdu Military Command General Hospital and Chengdu Bai'ao Biol-Tech (0, 0.1, 10, 1 000 n) was used to interfere cell differentiation for 3 days. Cultured cells were crushed with Tris-Triton X-100 PBS; activity of alkaline phosphatase was detected with Beckman CX-7 biochemical analytical device; effect of 100 g/L formalin, stained with Weigert-hematoxylin for 10 minutes, rinsed with water, differentiated with 5 g/L hydrochloric ethanol, stained with Van Gieson, desiccated with ethanol of the fractional volume of 0.95, cleared with dimethylbenzene with RT-PCR, Northern blot and Western blot during cell differentiation.protein.proliferated within 24-72 hours. Cells shaped as triangle, multiple angles and fusiform. Three days later, volume of adherent cells was increased and colony, and 10 days later, they confluenced. Bone marrow stroma cells in many generalight red to yellow). Red plasma presented synthesis of collagen. The deeper the red was, the more the collagens were.pression of PPAR-γ2 mRNA was (4.0±0.4)%, (1.7±0.2)% and (2.8±0.2)% (t=6.1, 7.2, 11.5, P< 0.01), which was higher crease the expression of PPAR-γ2 protein. When concentration of E2 was 0.1, 10 and 1 000 nmol/L, expression of PPAR-γ2 protein was (2.2±0.2)%, (2.6±0.2)% and (4.1 ±0.2)%, which was higher than that in 0 nmol/L E2 group [(1.2±0.10)%, t=6.6, 8.5, 13.2, P<0.01].CONCLUSTON: E2 can inhibit expression of alkaline phosphatase and promote differentiation of bone marrow stromal cells and expression of PPAR-γ2 mRNA and PPAR-γ2 protein.
3.Rehabilitation nursing for patients undergoing posterior lateral minimally invasive total hip arthroplasty
Yingqiong CHEN ; Qiaoxuan ZOU ; Shuxian CHEN ; Sisi XIE ; Xiaolian LI
Chinese Journal of Rehabilitation Theory and Practice 2006;12(1):84-85
目的探讨后外侧小切口行全髋置换术(THA)后患者康复护理。方法后外侧入路小切口患者45例48髋,经精心护理后与常规THA患者在手术切口、围手术期出血量、术后疼痛、住院天数、早期活动方面比较。结果在相同条件下,小切口手术患者减少围手术期出血量、术后疼痛、住院天数及术后早期康复快,缩短住院时间。结论康复护理有助于THA患者的恢复。
4.Synthesis and Identification of Hapten and Complete Antigen of Norketa mine
Tingting YANG ; Chuncheng ZHAO ; Xiaoping SHEN ; Bochang TAN ; Pankun ZOU ; Huaiming WANG ; Lingyun LIU ; Chao WEI ; Wanli WEI ; Jinhai HE ; Jie WU ; Dongsheng ZHANG ; Xiaolian ZHAO
Chinese Journal of Analytical Chemistry 2010;38(1):109-112
Under low temperature conditions, the hapten carboxyl-norketamine was synthesized by reacting norketamine and succinaldehyde acid.Identification result using electrospray ionization mass showed the hap ten was successfully synthesized.The artificial antigen confirmed by infrared spectroscopy was developed by conjugating hapten to carrier proteins with carbodiimide(EDC) method.Matrix-assisted laser desorption ioni zation time of flight mass spectrometry(MALDI-TOF-MS) showed that the ratio of hapten to BSA was 11:1.The antibody with high titer(5.12 × 10~4) was produced after immuning to rabbits.
5.Survey of nuclear medicine practice in Guangdong in 2016
Fan CUI ; Jianming ZOU ; Zhan TAN ; Weixu HUANG ; Yuxin JIA ; Xiaolian LIU ; Yanbing LIU ; Zixiang WU
Chinese Journal of Radiological Medicine and Protection 2019;39(6):454-459
Objective To survey the basic situation of diagnosis and treatment in clinical nuclear medicine across Guangdong province,evaluate the risks of exposure to the radiation workers and public and explore the countermeasures to control radiation exposre.Methods A survey team was set up to survey,by filling questionnaires,the basic information on nuclear medicine practices for workers,equipment,radionuclide,frequency and dose to workers and radiation protection measures.Results A total of 71 nuclear medicine institutions was involved in the survey with 733 radiation workers in 2016 in Guangdong.The average annual effective dose to nuclear medicine workers was (0.55±0.66) mSv per year.The total pieces of nuclear medical equipment was up to 189 in 2016 including 59 SPECT/CT scanners (5 SPECT),28 PET/CT scanners and 54 thyroid scanners.Total activity of 1.15× 10s MBq in radiopharmaceuticals was used in 325 903 examinations and treatments with the number of frequency of 2.97 examinations per 1 000 population.Concluions There have been a rapid progress in practice of nuclear medicine over the past 20 years in Guangdong province with departments of nuclear medicine set up in 18 of 21 cities.Compared with 1998,the activity in radiopharmaceuticals used has increased by 414% in 2016 and the number of frequency of examination and treatment has increased by 111%.The rapid expansion of nuclear medicine practice has also caused potential risks of radiation to the public and environment.
6.Epidemiological characteristics of coronavirus disease 2019 in Huzhou
JIN Meihua ; SHEN Jianyong ; FU Yun ; LIU Guangtao ; LIU Yan ; YANG Zhongrong ; REN Feilin ; XU Deshun ; CHEN Liqiang ; ZOU Yong ; DONG Xiaolian ; SHAO Bin ; LIU Bin
Journal of Preventive Medicine 2020;32(5):433-436
Objective:
To learn the epidemiological characteristics of coronavirus disease 2019(COVID-19)in Huzhou,so as to provide reference for prevention and control of COVID-19.
Methods:
All the confirmed cases of COVID-19 in Huzhou,diagnosed according to the COVID-19 Diagnosis and Treatment Plan(Sixth Version Trial)and reported from January 25 to February 7,2020,were recruited. The process of diagnosis and treatment,clinical manifestation,exposure history and close contacts were collected to analyze the epidemiological characteristics.
Results:
On January 25,the first confirmed cases of COVID-19 in Huzhou was reported. By February 7,totally 10 confirmed cases were reported and no asymptomatic infection was found. They were all imported,including three Wuhan residents,two with a trip to Wuhan,three with a trip to Suizhou,one with a trip to Hangzhou and one with a trip to Thailand(two Wuhan passengers on the same flight). The ratio of male to female cases was 1∶1. The median age was 32 years old. Seven cases were found when they went to a doctor by themselves,and three cases were found during the quarantine. The main clinical manifestations were fever,dry cough and fatigue. The median time from onset to diagnosis was 3 days. By March 3,all the cases were discharged,with median course of 24 days. There were 312 close contacts,and all of them were released after 14 days of quarantine.
Conclusions
To prevent imported cases from outside and stop spread inside taken by Huzhou government was proved to be effective. All the COVID-19 cases in Huzhou were imported,mostly from Wuhan. No local cases were reported.
7.Study on clinical management of HPV+/Pap-during cervical cancer screening
Aijuan HUANG ; Yun ZHAO ; Xiaolian ZOU ; Jin YAN ; Chao ZHAO ; Shuhui CUI ; Yanyan LI ; Lihua REN ; Jingran LI ; Mingzhu LI ; Yue WANG ; Jianliu WANG ; Lihui WEI
Chinese Journal of Obstetrics and Gynecology 2017;52(11):745-750
Objective To study the clinical management way for HPV+/papanicolaou (Pap)-during cervical cancer screening.Methods To analyze retrospectively the data from the patients who had loop electrical excision procedure (LEEP) for biopsy confirmed cervical intraepithelial neoplasia (CIN)Ⅱ in Peking University People's Hospital from Jan.2010 to Dec.2014.Results (1) For biopsy confirmed CINⅡ,HPV positive rate was 98.5% (135/137),Pap test positive [≥atypical squamous cell of undetermined significance (ASCUS)] rate was 69.3% (95/137),there was significant difference between them (x5=43.32,P<0.01).(2) For the 42 patients with HPV+/Pap-,whose cytology slides were reviewed again.Among them,the interpretations of there were 16 cases confirmed as the same before,while 26 cases were changed to abnormal (≥ASCUS).Cytology be misdiagnosed was 19.0% (26/137) at the first review.Among the 26 cases,13 (50.0%) cases were missed for the little amount of abnormal cells,8 (30.8%) cases for mild atypical morphology changed;the other 5 (19.2%) cases missed for stain problems.(3) For the cervical LEEP samples,37 cases of the pathology diagnosis were upgrade to CIN Ⅲ+,among them,2 cases of microinvasive cervical carcinoma,1 case of invasive cancer,34 cases of CIN Ⅲ;37 cases were CINⅠ or no lesion found;63 cases were still CIN Ⅱ.Four to six months later after LEEP,the cytology abnormal rate was 11.7% (16/137),and the HR-HPV positive rate was 34.3% (47/137).Conclusions Compared with cytology alone,cytology combined with HPV testing increase the sensitivity of cervical high grade lesion.For the cases of HPV+/Pap-cases,the cytology slides should be reviewed.The quality control of cervical exfoliate sample collection and interpretation should be strengthened.LEEP procedure is not only a treatment method,but also it could provide samples to confirm the diagnosis.
8.Analysis of Hot Spots in the Field of Pharmacy ——Taking China Pharmacy as Example
Mingwei LIU ; Yunzhu LIN ; Ping LIU ; Jing LIN ; Lijuan ZOU ; Xiaolian TANG ; Ju XIAO
China Pharmacy 2019;30(19):2712-2717
OBJECTIVE: To explore current hot spots in drug research and application,and provide reference of the draft use standard for pharmacy journal editor. METHODS: Taking China Pharmacy (short for our magazine) as example,all literatures published by our magazine and included by CNKI were searched during inception (Jan. 1990) and Mar. 19th, 2019; top 150 literatures [top 100 non-review literatures (NRA) and top 50 review literatures (RA)] were collected according to comprehensive score of downloaded number and citied number as indicators, and then analyzed in respects of publication year,column of NRA,first author’s work unit, subject words; one literatures which the cited number was largest was analyzed separately. RESULTS: Totally 150 literatures were published between 1993 and 2016, while the literature amount reached the maximum value in 2010 (18 articles). Among NRA, the most were in the columns of “pharmacy administration” (44 articles, 44.00%), “TCM and ethnic medicine” (18 articles, 18.00%), “pharmacists and pharmaceutical care” (14 articles, 14.00%). Among 150 first authors,73 (48.67%) were from college, 62 (41.33%) were from hospital. Subject words of NRA were “status and development” (20 times), “active ingredient” (14 times), “international experience” (9 times), while those of RA were “pharmacological action” (25 times), “Chinese herbal medicine” (15 times), “active ingredient” (12 times). The Principles and Utilization of Sequential Analysis of DDD System of Drug in Hospitals was cited for 1 426 times, which was much higher than the second one (261 times). CONCLUSIONS: This study can show the good literatures origin and study direction,provide reference to the hot spots for readers. At the same time, high-frequency cited literatures also suggests that researchers can conduct in-depth analysis of controversial and unclear contents in academic circles.