Objective To study the effects of mild hypothermia on endothelial cell of cerebral vessels in patients with moderate or severe cerebral infarction. Methods Sixty patients with moderate or severe cerebral infar-ction were divided into 2 groups: a mild hypothermia group (30 cases) and a non-mild hypothermia group (30 cases). Endothelin (ET), endothelial nitric oxide synthases(eNOS) and von Willebrand factor(vWF) were tested with RIA or ELISA. All the patients were treated with routine medication, in addition, those in mild hypothermia group were also treated with mild hypothermia. Results The ET and vWF levels of serum and cerebrospinal fluid (CSF) in patients of mild hypothermia group were significantly lower than those of non-mild pothermia group (P

Medical ultrasound imaging suffers from poor resolution due to its inherit imaging mechanism.In order to obtain the important diagnostic image in detail,restoration procedure needs to be performed.However,in practical ultrasound imaging,it's difficult to demonstrate the degradation process in detail.Therefore,blind image deconvolution is applied to estimate the original image from the degraded image with unknown point spread function or little available prior knowledge.In this paper,the main algorithms of medical ultrasound image restoration based on blind deconvolution are reviewed and classified into two categories,priori or joint with the image,according to the identification methods,and the basic theories and improved methods are analyzed and discussed.Finally,the future directions in blind restoration for medical ultrasound images are proposed.

Vascular construction is an important content in tissue engineering domain,in vitro implanting seed cells on absorbable scaffold materials to form multi-layered cellular layer and establish autoblood vessels along the scaffold absorption. The tissue-engineered vessels are characterized by high biocompatibility and plasticity,less foreign body reaction,and no thrombus production and infection; in addition,the vessels may finally become autoblood vessels in the complete significance. In this article,artificial vascular materials,scaffold processing methods,and vascular culture of seed cells were compared to explore an ideal method to make artificial vessels.

Up to now,there are 21 ministry of education innovation teams established by 37 independent medical colleges and universities,which has become an important part of the medical education system innovation team building.From the perspective of SWOT (strengths,weaknesses,opportunities,threats) analysis,in the process of ministry of education innovation team establishment in the independent medical colleges,there are unique advantages,followed by obvious disadvantages,and there are favorable outside opportunities,accompanied with unprecedented challenges.Independent medical colleges shall keep up with the development trend,leverage the national regional development policy,give full play to the advantages,endeavor to overcome weaknesses,seize the opportunities and face the challenges actively,make the right strategic decisions,in order to better play an irreplaceable role in higher medical education system.

0.05).In the ineffective group,CCI was 32.2%,in which positive LCT was 57.9%.In the effective group,CCI was 67.8%,in which positive LCT was 12.5%,and there was significant difference in LCT positive rate between the ineffective and effective groups (? 2=13.43,P

Nonaqueous capillary electrophoresis-chemiluminescence (NACE) method was developed for the determination of five phenolic environmental estrogens including bisphenol A (BPA),4-nonylphenol(4-NP) ,4-tert-octylphenol(4-tOP),4-tert-butylphenol(4-tBP) and tetrabromobisphenol A(TBBPA) in food packaging material. Target compounds in soaking solution of food packaging material samples were separated by NACE-CL after derivatization with 4-(4,5-diphenyl-lH-imidazol-2-yl) benzoyl chloride ( DIB-Cl) ,reacted with per-oxyoxalate chemiluminescence system,and then detected through magnifying light signal with photomultiplier. With 17β-E_2 as internal standard,qualitative and quantitative analysis of the target compounds were performed by relative migration time and the ratio of relative chemiluminescence intensity,respectively. Several influence factors on separation with NACE,such as the composition and proportion of organic solvent,the concentration of electrolyte,temperature surrounding of the capillary,the concentration of acetic acid and work voltage,were investigated. And the peroxyoxalate chemiluminescence system was optimized. Under these conditions,4-tBP,BPA,4-OP,4-NP and TBBPA were all separated and showed good linearities in the ranges of 0.0095-3.0 mg/L,0.0087-3.0 mg/L,0.0085 -3.0 mg/L,0.011 -3.0 mg/L,0.009-3.0 mg/L,respectively,with correlation coefficients over 0. 9947. The RSDs of relative migration time and relative chemiluminescence intensity ratio were 0.9% -3.0% and 6.5% - 8.6%. Recoveries for the spiked samples ranged from 86.7% to 98. 8%. Five common food packaging material samples were analyzed. The proposed method is simple and sensitive for the quantitative determination of phenolic environmental estrogens in food packaging material samples.

The effect of recombinant human phospholipase D2(rhPLD2)in vivo was investigated on the secretion of serum glycosyl phosphatidylinositol-specific phospholipase D(GPI-PLD)in guinea pigs of chronic asthma．Ater treating the guinea pigs attacked by chronic asthma with rhPLD2,the GPI-PLD activity detection was canrried out by phase separation of human placental alkaline phosphatase in Triton X-114．Compared with the healthy guinea pigs(NS group),the serum GPI-PLD in the guinea pigs of chronic asthma are much higher than that of control groups,P≤0．01．Our results showed that rhPLD2 could significantly reduce the secretion of GPI-PLD when the guinea pigs were attacked by chronic asthma．

Objective To explore the relationship among psychological empower,role stressors and job burnout in doctors.Methods According to convenience sampling principle,350 doctors were selected and assessed with the job burnout scale (MBI-GS),the psychological empower scale and role stressors scale.Results The scores of meaning(10.80±3.12) and self-determination (9.33 ± 3.19) were negatively correlated with the scores of job burnout (r value was from-0.28 to-0.47,P＜0.01),the scores of self-efficacy(11.42±2.67)were negatively correlated with the scores of cynicism (1.61 ± 1.21)and reduced personal accomplishment (3.62±1.51)(r=-0.23,-0.51,P＜0.01),and the scores of impact(8.04±3.18) were negatively corrclated with the scores of emotional exhaustion(1.89±1.19) and cynicism(r=-0.17,-0.25,P＜0.01).The scores of role stressors were positively correlated with the scores of emotional exhaustion and cynicism(r=0.15-0.45,P＜0.01),and the scores of role ambiguity(19.09±3.76) were positively correlated with the scores of reduced personal accomplishment (r=0.42,P＜0.01).The self-efficacy and self-determination can negatively predict job burnout(P＜0.01) ;the role overload(15.24±4.62) and role ambiguity can positively predict job burnout(P＜0.01).Conclusion Psychological empower and role stressors are closely related with job burnout,and have significantly predictive effects on job bumout.

The research of structural biology is closely related to the field of biotechnology.Carrying out the second class of structural biology will be helpful in learning theoretical knowledge of biotechnology and in improving the teaching quality.Based on our experiences of carrying out the second classroom activities among students majoring in biotechnology,we believe that the selected teaching contents,reasonable classroom design,sufficient preparation before the class,flexible teaching methods and objective after-school summaries are essential for improving the teaching quality.

This study examined the effect of IL-10 on immunoglobulin-like transcript (ILT4) expression of human monocytic leukemic cell line THP-1, especially the role of the ILT4 promoter activity. ILT4 promoter area was amplified by PCR, and was cloned into the eukaryotic expressing vector pGL3-Basic. The pGL3-ILTP obtained was tested by double endonuclease digestion and sequencing. Then, the recombinant plasmid was transfected into THP-1 cells by using lipofectamine. After culture with IL-10 for 12 h, the mRNA extracted from THP-1 cells was detected by RT-PCR and the protein was detected by FACS. The dual-luciferase reporter assay system was employed to detect the activity of ILT4 promoter with or without IL-10. The results showed that the activity of pGL3-ILTP was significantly increased and was more than ten times that of pGL3-Basic cells. After culture with IL-10 for 12 h, the expression of ILT4 protein and its mean fluorescence intensity (MFI) were increased. Moreover, the mRNA was remarkably higher than that of the control group. Dual-luciferase reporter assay revealed that ILT4 promoter was much more activated after being treated with IL-10. We were led to conclude that pGL3-ILTP containing ILT4 promoter was constructed successfully. The expression of ILT4 could be up-regulated by IL-10 both at the transcriptional and translational level. Furthermore, ILT4 promoter could be much more active after addition of IL-10. This study suggests that IL-10 up-regulates ILT4 expression on monocytes via increasing ILT4 gene promoter activity, which may have implication for inducing transplantation tolerance in clinical practice.