OBJECTIVE: To investigate the protective effects of tea polyphenol (TP) on lung injury caused by global cerebral ischemic-reperfusion and its mechanism. METHODS: Improved Pulsinelli-brierley method was applied to induce damage model by global cerebral ischemia model for 15 min and global cerebral ischemia-perfusion for 3 h. Some indexes were observed, including morphology change of cerebral and lung tissue, activity of superoxide dismutase (SOD), concentration of malondialdehyde (MDA) and expression of lipopolysaccharide receptor CD14. Healthy rats were divided into sham group, model group and nimodipine group. RESULTS: As compared with sham group, there were pathologic damage of lung and brain and the positive expression of CD14 cell of lung in model group. As compared with model group, the activity of SOD were increased significantly (P

Objective To investigate the clinical characteristics and diagnosis and treatment of ileocecal disease.Methods The general data,clinical manifestations,imaging examinations,colonoscopy and pathological examination and diagnosis of 134 patients in our hospital with ileocecal disease from September 2009 to March 2016 were collected.The clinical characters were summarized by retrospective analysis.Results A total of 134 cases with ileocecal disease were collected,and 36(26.87％)of them were ileocecal cancer,30(22.39％)of them were inflammatory bowel disease,26(19.40％)of them were intestinal tuberculosis.The main clinical manifestations of ileocecal lesion were abdominal pain,abdominal distention,diarrhea,bloody stool,etc.The colonoscopy imaging showed mucosal hyperemia,edema,erosion,ulcer and tumors on the intestinal tract.The benign lesions mainly showed ulcer with mucosal erosion and edema,and the ileocecal cancer showed neoplasm.CT examination was the common imaging method,and 93(69.40％)cases got tested.49 cases had operation,in which 7 cases had misdiagnosed before operation,and the rate of misdiagnosis was 14.28％.Conclusion The ileocecal disease is mainly benign lesions,and the process of diagnosis is complex;the rate of misdiagnosis is a little high.

Objective:To construct the fingerprinting and analyze the genetic relationship in Gastrodia elata Bl.Methods:Inter-simple sequence repeat(ISSR) markers were used to investigate 23 populations of wild and cultivated Gastrodia elata Bl.in Guizhou Province.4 effective primers which were screened from 24 ISSR primers detected 32 loci in 23 samples,29 loci were polymorphic,amounting to 90.63% of the total loci..The average number of bands was 8 and the percentage of polymorphic loci(PPB)was from 80% to 100%.UPGMA dendrogram was analyzed by NTSYSpc,ver.2.2.Results:Similarity coefficients of the 23 populations were from 0.13 to 1.00.Individuals of the same population did not strictly cluster together and the genetic diversity of individuals in different populations was considerably high.Conclusion:Geographical distribution,variant growing conditions and artificial selection were significant factors for the rich genetic polymorphism of Gastrodia elata Bl.

Objective To observe the effect of Tanshinone ⅡA on proliferation and apoptosis of human gastric adenocarcinoma cell line SGC-7901 and its mechanism.Methods The human gastric adenocarcinoma cell line SGC-7901was cultured in vitro and with Tanshinone ⅡA at various concentrations.Cytotoxicity was valuated by MTT assay,apoptosis-related alterations in morphology were ascertained by transmission electron microscopy.The cell cycle distribution and the apoptotic rate were investigated by flow cytometry.SGC-7901 cell apoptosis with nuclear chromatin concentration and fragmentation as well as cell shrinkage and the formation of apoptotic bodies were observed by TEM.Results The growth of SGC-7901 cells was inhibited significantly in a dose-dependent manner.Tanshinone ⅡA could induce the apoptosis in time-dependent manner.Conclusion Tanshinone ⅡA has effect in inhibiting SGC-7901 cells and inducing the apoptosis.The apoptotic rate and percentage of cells in G1 phase increased in the SGC-7901 cells treated by Tanshinone ⅡA.

Objective To investigate the effects of airborne fine particulate matter(PM2.5) on anti-oxidative enzymes activities and lipid peroxidation levels in livers, spleens, and kidneys of rats. Methods 32 male Wistar rats were randomly divided into PM2.5 exposure groups of different concentration (1.5, 7.5, 37.5 mg/kg), exposed by tracheoperfusion and control group treated with physiological saline. Rats were killed 24 h after treatment, and the levels of thiobarbituric acid reactive substance (TBARS), glutathione(GSH) and activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) were determined. Results PM2.5 exposure caused significantly decrease of SOD, CAT, GSH-Px, SOD/TBARS in livers and kidneys in a dose-dependent manner compared with control group (P

ObjectiveTo study wether muscle spindle afferents have the regulating effects on res piratory movement. MethodsExperiments were performed on 36 anesthetized, immobilized, bilater ally vagotomized and artificially ventilated rabbits. Using the phrenic nerve discharge as the respira tory index, this experiment have assessed the effects of muscle spindle afferents invoked by intraarte rial injection of succinylcholine(Sch) on respiration. Results①In 40(63.49% ) cases which had the facilitated effects, 23 (36.51%) cases had the effect of prolonged inspiratory phase and 17 (26.98 % ) cases had the effect of attenuated expiratory phase and augmented respiratory frequency. In addition,there were 11(17.46% ) cases which had the attenuated effects,and 4(6.35% ) cases which had the biphasic effects,and 8(12.7% ) cases which had no effects;②The effect of prolonged inspiratory phase mainly showed the prolongation of inspiratory duration(Ti), but the effect of at tenuated expiratory phase and augmented respiratory frequency mainly showed the decrease of expi ratory duration (Te) and the augmentation of respiratory frequency(RF) ;③The above facilitated ef fects of Sch were significantly attenuated by intramuscular application of bupivacaine to destroy the muscle spindles. ConclusionThese results suggest that muscle spindle afferents induced by intraar terial injection Sch have a significant facilitated effect on respiration.

AIM: To observe the difference in the application of proportion of pinellisa decoction for purging stomach-fire, ginger rind and licorice root with different BP (back propagation,BP) method of artificial neural network. METHODS: The influence of gastric mucuqas content in normal rats was measured, and the groups of different flavor of a drug and dosage were divided according to homogeneous design. This research was programmed with MATLAB6.5, conformed the experimental data with BP artificial neural network, and the research compared the different structural topology of 8-3-1?8-8-1?8-12-1, and compared the different influence with the different BP method to network. And then, the expected mode of gastric mucus content was formed, which based on the BP neural network. RESULTS: The artificial neural network model that had 8-10-1 structural topology and improved BP method was formed, and it was a kind of good method to uniform the studied samples and to forecast the unstudied samples, Moreover, the expected effect of variable learning rate back propagation was best. CONCLUSION: Using the studying of pinellisa decoction for purging stomach-fire, ginger rind and licorice as an example, and give birth to the new model in studying proportioning of compound prescription. This model will found its position in studying proportioning of compound prescription.

BACKGROUND:With the improvement of in vitro fertilization-embryo transfer technology, patients with in vitro fertilization-embryo transfer are facing more and more psychological stress. To completely understand their mental features and to explore effective mental intervention are important problems faced workers of reproductive medicine.
OBJECTIVE:To investigate the psychological condition of in vitro fertilization-embryo transfer patients.
METHODS:A total of 92 in vitro fertilization-embryo transfer patients, who entered the cycle from October to
November 2012, were enrol ed in this study. The psychological conditions of these patients were evaluated using Symptom Checklist-90.
RESULTS AND CONCLUSION:Al the factor scores of the Symptom Checklist-90 in patients with in vitro fertilization-embryo transfer achieved significantly higher levels than national adult norm on their first day of in vitro fertilization-embryo transfer cycles (P<0.05), with the exception of Psychiatric state factor. Meanwhile, in addition to Psychiatric state, differences of al the factors of Symptom Checklist-90 in patients with in vitro fertilization-embryo transfer were statistical y significant in different stages of in vitro fertilization-embryo transfer cycles at the first day of entering cycle, the day of obtaining ovum, and the seventh day of embryo transfer, (P<0.05). Interpersonal relationship, Depression and Anxiety scores were increased at the first day of entering cycle, the day of obtaining ovum, and the seventh day of embryo transfer (P<0.05). These results showed that patients with in vitro fertilization-embryo transfer tend to have poor psychological status, which is a problem in patients during the different stages of in vitro fertilization-embryo transfer cycles and becomes aggravated during egg retrieval and waiting for the outcome. Interventional measures should be taken in different stages to improve patients’ mental health.

Objective To reveal the incidence and types of acid-base disorder (ABD) of critically ill patients in emergency.Methods The clinical data of critically ill patients in the resuscitation room were collected prospectively from December 1,2008 to March 31,2009.Both arterial and venous blood samples were collected to detect arterial blood gas analysis and serum electrolytes simultaneously and their ABD were analyzed.Results Of totally 766 cases,the incidence of ABD was 97.3％ (745 cases).Among ABD the simple acid-base disorder ( SA BD) was 149 cases ( 20.0％ ),dual acid-base disorder (DABD) 525 (70.5％ ) and triple acid-base disorder (TABD) 71 (9.5％ ).After calculating anion gap (AG),the incidence of TABD increased from 12 to 71 cases and the missed diagnosis rate were 83.1％ (59 cases) and after calculating potential HCO3-,the incidence of TABD increased from 8 to 71 cases and the missed diagnosis rate were 88.7％ (63 cases).The age of patients with TABD (77.9 ± 10.7 years old) was older ( P ＜ 0.01 ) than that of other two groups of SABD and DABD as well as APACHEⅡ score (25.9 ± 7.1 ) and incidence (22.5％ ) of MODS in TABD were higher (P ＜0.01 ).The mortality of patients with TABD on the first,second,third and seven day were 14.1％,23.9％,26.8％ and 38.0％ respectively and significantly higher than those in SABD and DABD (P ＜0.05 or P ＜0.01 ).Conclusions The incidence of ABD was 97.3％.Calculation of AG and potential HCO3- would help find metabolic acidosis and TABD.The age and APACHEⅡ score were key factors to poor prognosis of patients with TABD.

A sandwich ELISA for the measurement of urokinase(UK)antigen was developed based on anti—UK monoclonal antibodies(McAbs)against two non—overlapping epitopes.The lower limit of sensitivity of the assay was 0.15ng/ml.Coefficients of variation of the assay at physilogi-cal levels of UK were 4.3 percent within assaysw and 8.7 percent between assays.The recovery of added UK was about 98 percent.Culture media of some human malignant cell lines contains more UK than that of normal cell lines measured by our assay.The ELISA was used to measurethe concentration of UK in plasma from 82 healthy donors.The mean value for the healthy donors was 1.31?60.6ng/ml of UK in plasma.