Objective:To study the biologic characters of human foreskin fibroblasts(HFF)to lay the foundation for a long-term culture for human embryonic germ cells in vitro.Methods:The method of separating HFF was investigated.By immunocytochemical method,MTT and flow cytometry,we investigated the morphology and the purity of HFF,study the cell growth curve and cell cycle of HFF,and the effects of different concentrations of mitomycin on growth of HFF at different times.Results:Epidermis and dermis were more easily separated after overnight digestion(12~16 h)in 0.25% Trypsin at 4℃,compared with incubation for 2 h in 5% CO2 at 37℃.Vimentin was strongly positive stained with the purity of 95 percent.There was no obvious stranded period.The rapid proliferation period was in 1~6 d of cell growth curve.The platform stage began at 7 d.Majority of the cells were in G1/G0 phases.The cell cycle corresponded to cell growth curve.Mitomycin C(MMC)inhibited the proliferation of HFF at 12.5 ?g/ml over 2.5 h of MMC treatment.Conclusion:Its biological characters showed that HFF can be used as feeder layer for in-vitro cultured human embryonic germ cells。

Analysis of differences between traditional Chinese medicine (TCM) philosophy with malnstream western medicine was done with reference to intercultural communication theory. It was found that the differences are malnly manifested through holism philosophy of TCM and dualism of Western medicine, intuition thinking mode of TCM and mechanical thinking mode of western medicine, and ideographic Chinese language and objective description of the Western medicine. The translation principle was also discussed with the perspective of intercultural communication. It is of great significance to respect the recipient’s privilege, to create intimate text, and to understand the bilingual differences if TCM translation is to be promoted.

4.0 mg/L) fluorosis areas in 2008.The fluoride content of the drinking water,the chlidren's dental fluorosis and urinary fluoride were investigated.Results The fluoride in drinking water in the water improved areas was(1.4?0.8)mg/L,which was significantly lower than that in the non-water improved areas(2.6?1.5)mg/L(P

Estrogen has many physiological functions. It can prevent atherosclerosis, coronary heart disease and osteoporosis etc. Phytoestrogen is a kind of natural compounds in plants that is structurally similar to estrogen and has estrogen-like effects. The studies on the effects of estrogen and phytoestrogen on the hypoxia at present is limited and its mechanism is being researched.

Objective To establish an analytical method for fingerprint of Citri Reticulatae decoction pieces by HPLC. Methods Thermo C18 chromatographic column (4.6 mm×250 mm, 5μm) was used with methanol-0.3%phosphoric acid in gradient elution. The flow rate was 0.8 mL/min, the detection wavelength was set at 280 nm, and the temperature was 40 ℃. The similarities of the 15 batches of samples were compared by similarity evaluation, cluster analysis and principal component analysis. Results Based on the fingerprints of Citri Reticulatae decoction pieces, 8 common peaks were identified. The similarities were all more than 0.9 among all batches. 15 batches can be divided into three categories. Conclusion The method is simple and steady. It provides a basis for the quality control and evaluation of Citri Reticulatae decoction pieces.

120 cases of nuchal ligament strain were treated with Tuina at the nape, tapping Dazhui (GV 14) with plum-blossom needle, and cupping Dazhui(GV 14). Clinical observation was made after 14-day treatments. Eighty-seven cases got cure, 28 cases improvement and 5 cases failure; the total effective rate was 95.8%.

Objective To study the effective constituents from the stem bark of Erythrina variegata.Methods The compounds Ⅰ-Ⅸ were isolated by a combination of silica gel,Sephadex LH-20,and ODS chromatographies and their structures were identified by spectral methods.Results Nine known compounds were isolated from the stem bark of E.variegata.Their structures were identified by means of spectroscopic analysis as: 5,4′-dihydroxy-8-(3,3-dimethylally)-2″,2″-dimethylpyrano [5,6∶6,7] isoflavone(Ⅰ),stigmasterol(Ⅱ), erythrinasinate B(Ⅲ),3-hydroxy-2′,2′-dimethylpyrano [5,6∶9,10] pterocarpan(Ⅳ),5,7-dihydroxy-8-(3,3-dimethylally)-dihydroflavanone (Ⅴ),5,4′-dihydroxy-2″,2″-dimethylpyrano [5,6∶6,7] isoflavone(Ⅵ),5,7,4′-trihydroxy-6,8-di(3,3-dimethylally) isoflavone(Ⅶ),5,2′,4′-trihydroxy-8-(3,3-dimethylally)-2″,2″-dimethylpyrano [5,6∶6,7] isoflavone(Ⅷ),5,4′-dihydroxy-6-(3,3-dimethylally)-2″,2″-dimethylpyrano [5,6∶7,8] isoflavone(Ⅸ).Conclusion Compounds Ⅰ,Ⅲ,Ⅳ,Ⅶ,and Ⅸ are isolated from this plant for the first time and compound Ⅴ is newly isolated from the plant of Erythrina Linn.

Objective To investigate the effect of exception questions/miracle questions in SolutionFocused Approach in nursing on affects,confidence to solve problem,the perception of problem's difficulty,and actual solving problems,to provide practical basis for practitioners of Solution-Focused Approach.Methods A total of 102 nurses were randomly divided into exception questions in Solution-Focused Approach group (exception group),miracle questions in Solution-Focused Approach group (miracle group)and cause questions in the traditional psychotherapy pattern group(cause group).The difference among three groups on the aspect of the change of affects,confidence to solve problem and the perception of problem's difficulty,and the help form the questions immediately and after 1 week (immediate help and 1 week after help) were studied.Results Eventually subjects effectively,34 nurses in exception group,31 nurses in miracle group,31 nurses in cause group.The difference among three groups in aspect of the change of affects,the change of confidence to solve problem,immediate help and 1 week after help were statistically significant (Z=9.68-18.58,P＜ 0.05).Furthermore,change in problem solving confidence on exception group was higher than cause group(51.9 vs.35.6,P ＜ 0.05),change in affects on exception group and miracle group were higher than cause group (53.8,55.1 vs.36.0,P ＜ 0.05),the immediate help on exception group and cause group were higher than miracle group (59.8,52.5 vs.32.0,P ＜ 0.05),and 1 week after help on exception group was higher than miracle group and cause group(64.5 vs.42.3,37.0,P ＜ 0.05).Conclusions Exception/miracle questions can both improve the emotional state,and exception questions can enhance the confidence to solve problems and provide more help for nurses.

Lung cancer is one of the most common malignant tumors in the world .However,its pathogen-esis is still unclear .With the deep research of related signal pathways underlying the development ,progression and prognosis of lung cancer , we find that the mTOR signaling pathway , JAK-STAT pathway , Notch signaling and IGF have been identified to play a major role in the growth ,proliferation,differentiation,apoptosis and invasion of tumor cells.Therefore this paper is to review the effects of the above several pathways ,which may prove a new ba-sis for the pathogenesis and therapy of lung cancer .