Objective To investigate psychosocial distress status of hospitalized patients with nasopharyngeal carcinoma and then analyze its influencing factors.Methods Using the method of convenience sampling,the level of psychosocial distress of hospitalized patients with nasopharyngeal carcinoma was measured with psychosocial distress thermometer (DT),then by orderly classification Logistic regression analysis methods,the main factors affecting the degree of psychosocial distress were obtained.Results This study in cluded 235 patients,and 228 patients completed the survey effectively,DT score was (4.030±2.993) points,including DT ＜ 4 points (mild pain) accounted for 36.8％ (84/228),DT 4-6 points (moderate pain) accounted for 38.2％ (87/228),DT≥7 points (severe pain) accounted for 25.0％ (57/228).Regression analysis showed that the economic problems,emotional problems (depression,fear,sadness),body problems (eating,oral ulcer,sleep problems),age,education degree,stressful life events in the recent year,the time of disease diagnosis,and disease recurrence were the main factors influencing the degree of psychosocial distress.Conclusions Hospitalized patients with nasopharyngeal carcinoma have widespread psychosocial distress,clinical medical personnel should take individualized psychological evaluation and intervention according to the related factors of psychosocial distress in order to reduce the degree of psychosocial distress.

Objective To evaluate the validity and reliability of social avoidance and distress scale (SAD) for conforming it' s applicability in Mongolian college students.Methods Social avoidance and distress scale (SAD)security scale (SQ),self-esteem scale (SES) and life satisfaction scale (AULC) was used to measure 399 undergraduates whose can understand Mongolian and Chinese language.Results The total scale reliability was 0.872,avoidance scale was 0.726,distress scale was 0.742,total scale heavy reliability (r) was 0.86,avoidance scale (r) was 0.789,distress scale (r) was 0.814.(2)the confirmatory factor analysis verified the effectiveness of the structure,the revised model fitting index x2/df=4.864,NFI =0.92,TLI =0.90,GFI =0.90,IFI =0.87,GFI =0.92,AGFI =0.93,RMSEA =0.06,model fitting was better.(3) There was a significant positive correlation between avoidance scale and distress scale (r=0.634,P＜0.01),and there was a negative correlation (r=0.202-0.499,P＜0.01) between avoidance scale and SES,SQ and AULC.There was a negative correlation between distress scale and SES,SQ and AULC (r=0.192-0.556,P＜0.01).Conclusion SAD (Chinese version) has a good reliability and validity in Mongolian college students.

As the products of modern science,the effects of video games on children and adolescents drew the attention of psychologists.Anderson and Bushman proposed the General Aggressive Model,which could explain both the development of aggressive behavior and the difference of individual vulnerability to the effects of violent video games,and they advocated the effects,including short-term effects and long-term effects.Many researches about the relation of violent video games and aggression reported five consistent results:exposure to violent video games increased aggressive behavior,cognition,affect and physiological arousal,and decreased helping behavior.The positive intervention of parents can decrease negative effects of video games.

Fecal microbiota transplantation(FMT),as a special organ transplantation,can be used to reconstruct the intestinal flora.It has been concerned by clinical practice,but it has not become a routine treatment method.With the gradual deepening of the study of FMT,the refractory difficile fusiform bacillus infection,inflammatory bowel disease,irritable bowel syndrome,chronic fatigue comprehensive syndrome and some metabolic disorders related to intestinal diseases were obtained using.In recent years,there are some reports about the application of FMT in children's diseases.Combining with the relevant literature at home and abroad,this paper reviews the indications and the taboo of the application of FMT in children,the aim is to provide a reference for FMT to be better applied in pediatric clinical.

Objective:To study the fracture resistance of nanofilled composite resin restoration for the treatment of incisally fractured in-cisors. Methods:30 extracted permanent maxillary central incisors were randomly divided into A, B and C groups (n=10). In group A and B, the teeth were cut obliquely from the mesial incisal angle. Labial reduction of 0. 5 mm for veneer and dovetails in the lingual surfaces were prepared. In group A, additional 2mm of the incisal edge were cut horizontally. The teeth were restored with nanofilled com-posite resin. The samples in group C were unprepared as the controls. The specimens were stored in artificial saliva and underwent 2 000 cycles of thermal cycling test. Fracture resistance of the teeth was measured by a test machine and the fracture mode was observed. Re-sults:The mean fracture load(N) of group A, B and C was 466.93 ±171.68, 444.5 ±173.00 and 474.77 ±97.55 respectively(P>0. 05). All fractures in the 2 test groups but 1 in the control group were in a favorable way. Conclusion:Incisally fractured incisors restored with nanofilled composite resins can achieve the fracture resistance similar to that of intact natural tooth. Failure modes of the restored teeth are in favorable way. Additional cut of 2 mm of the incisal edge dose not affect the fracture resistance of the restored teeth.

Objective To compare the effect of different cryoprotectants and different concentrations on controlled?rate freezing of human ovarian tissues. Methods Ovarian tissues were sampled from 15 patients undergoing benign ovarian tumor surgery. Cortical slices were frozen by controlled?rate freezing using three cryoprotectants,propanediol,ethanediol,and dimethylsulphoxide,and the concentration of each cryoprotectant was 1.5 mol/L or 2.0 mol/L. Cortical slices obtained from each patient were processed with each cryopreservation procedure simultaneously. Morphology of fol?licles was studied by light and electron microscopy and the normal rate was compared with that of the fresh tissues from the patient. Results There were no significant differences in distribution of follicles of different developmental stages between each group(P<0.05). Light microscopy showed 1.5 mol/L EG and 2.0 mol/L EG groups had the best freezing effect,and the difference in the morphologically normal rate of follicles was not statisti?cally significant compared to fresh controls(P>0.05). However,the difference was statistically significant for 1.5 mol/L PROH,2.0 mol/L PROH, 1.5 mol/L DMSO and 2.0 mol/L DMSO groups(P<0.05). Electron microscopy showed the oocyte quality declined after controlled?rate freezing pro?cedure. However,the statistical analysis was not conducted due to little data. The viability of granulosa cells was significantly declined after all the freezing procedures compared to that of the fresh control tissues(P<0.01). The number of morphologically normal granulosa cells was slightly higher in the tissues which had been cryopreserved with 2.0 mol/L PROH and 1.5 mol/L EG,but no significant differences were found between any of two frozen groups(P>0.05). Conclusion Controlled?rate freezing using 1.5 mol/L EG as the cryoprotectant can better save oocytes and granulosa cells. It is a preferable freezing procedure for ovarian tissues.

Objective To study the clinical effect of tacrolimus combined with small dose of prednisolone in treatment of IgA nephropathy.Methods 84 cases with IgA nephropathy were randomly divided into study group and the control group in accordance with the digital table method.The control group was given simple hormone treatment, while the study group was treated with tacrolimus combined with small dose of prednisolone.The clinical efficacy and safety were compared between the two groups.Results The treatment effective rate (100.00%)and complete remis-sion rate (73.81%)of the study group were significantly higher than those in the control group (76.19%, 23.81%),and the differences were statistically significant (χ2 =21.01,11.35,P <0.05 ).After treatment,24h urinary protein level was significantly decreased compared with before treatment.24h urinary protein level[(0.54 ± 0.41)g/L]of the study group was significantly lower than the control group[(1.06 ±0.62)g/L],and the difference was statistically significant (t =4.01,4.58,3.87,all P <0.05).In addition,before and after treatment,the serum creatinine,blood sugar,white blood cells,cereal third transaminase,SBP and DBP levels between two groups had no significant differences (P >0.05).When patients lighter (Lee's Ⅱ level),the team for complete response rate was significantly higher than the control group,and the difference was statistically significant (χ2 =5.01,P <0.05 ). However,with the aggravation of pathological damage (Lee),the effective rate and complete remission rate of the study group and the control group showed a downward trend.The effective rate and complete remission rate of the study group were higher than those in the control group,but the differences were not statistically significant (χ2 =1.03,2.11,1.20,1.20,all P >0.05).The incidence rate of adverse reactions in the study group (19.05%)was not significantly different from that of the control group (16.67%)(χ2 =0.08,P >0.05 ).Conclusion Tacrolimus combined with small dose hormone for the treatment of IgA nephropathy has high clinical value,it can significantly reduce the level of IgA nephropathy 24h nephropathy,and adverse reactions.

Objective To provide reference basis for clinical ,it is necessary to analyse the distribution and drug resistance of pathogenic bacteria isolated from intensive care unit .Methods The bacterial specimens from patients that treated in intensive care unit from January to December in 2013 ,had been collected and cultivated .The VITEK automatic bacterial identification system was performed to identify pathogens and drug susceptibility test .The software of Whonet5 .6 was used for statistically analysis .Results 1261strainsofpathogenicbacteriawereisolated,including900strainsofgramnegativebacilli,348strainsofgrampositivebac‐teria and 13 of fungi .Conclusion Gram negative bacillus is the main pathogenic bacteria in the critical patients .In order to improve the clinical efficacy ,clinical treatment should be based on the pathogenic bacteria culture and drug sensitivity test results to reasona‐bly apply antibacterial drugs .

Objective To evaluate the reliability and validity of the short form of the Chinese Childbirth Self-Efficacy Inventory (CBSEI-C32) in Haikou,analyze the influencing factors of pregnant women's self-efficacy.Methods The CBSEI-C32 was used to investigate the self-efficacy of the 138 pregnant women in Haikou.Cronbach's α coefficients and confirmatory factor analysis were used to evaluate the reliability and validity of the questionnaire,the influencing factors were analyzed by the univariate analysis and multiple regression analysis.Results The Cronbach's α coefficients for the scale and subscales were 0.973 and between 0.971 and 0.976 respectively;and the cumulative variance was 82.60％,which indicated the scale's reliability and validity was good.The pregnant women's self-efficacy score was (189.64±36.54) points,The times of childbirth,family function and the main coping methods were the influence factor.Conclusions CBSEI-C32 was suit to measure the confidence of natural childbirth in Haikou pregnant women,and their self-efficacy level was general,diversity nursing intervention had to apply for improve pregnant women's self-efficacy.

Objective To investigate the effect of rAAV2 VEGF 165 (recombinant adeno associated Virus 2 vector encoding VEGF 165 cDNA) in stimulating angiogenesis, its dose effect relationship in swine acute coronary occlusion model, and to search for a suitable dose for further investigation in swine myocardium ischemia model Methods Ligation of LAD (left anterior descending coronary artery) was performed in swine modles via mini thoracotomy rAAV2 VEGF 165 (experimental group) or phosphate buffer saline (control group) was injected around the infarcted region The experimental group was divided into four subgroups: according to the amount of virus genome, which were: 1?10 11 v g (virus genome), 5?10 11 v g, 1?10 12 v g and 5?10 12 v g Animals underwent angiographic evaluation of collateral development four weeks after the operation Expression of VEGF was assessed by semiquantitative RT PCR and immunohistochemical staining Results Four weeks after the operation, expression of VEGF was detected in all the four experimental subgroups and the level of expression was higher than that of the control group Capillary density was higher in both of the 1?10 12 v g and 5?10 12 v g subgroups, compared with the control Coronary angiography demonstrated higher collateral index to the occluded LAD in the groups of 5?10 11 v g,1?10 12 v g and 5?10 12 v g than that of the control Conclusion Direct injection of rAAV2 VEGF 165 can achieve effective expression of VEGF in swine acute ischemic myocardium At certain dosage, rAAV2 VEGF 165 can promote generation of blood ressels in swine acute ischemic myocardium and 1? 10 12 v g is a more suitable dosage for swine myocardium ischemia model