Objective To observe the possibility and efficacy tolerability of topiramate(TPM) in transferring monotherapy for the patients wi th partial seizures with or without secondary general seizure,as well as to find out the optimum way of the transferring.Methods After T PM add-on therapy 64 patients with seizures reduced ≥50%, it reduced about 1/ 4 of AEDs or one drug each time according to every two weeks until gradually to stop fully and transferring monotherapy.Results 18 patients we re successful with TPM monotherapy (28.13%), 35 patients were half success(54.69 %), 11 cases for failure (17.19%). The less AEDs combined, the higher possibilit y of transferring monotherapy would be. The rate of success for cabamazepine see med higher than valproate.Conclusion It was possible to transfe r monotherapy by TPM. The rate of success was 28.13%. It was easier to reduce ca bamazepine than valproate. In the transferring monotherapy it was better to redu ce one after another, in principle the one of the little dose was reduced first, and then the hepatic enzyme inducing agents was reduced. Even if the transferrin g was half success,the kinds of concomitant AEDs could be reduced at least, it w as in line with the policy of medicine application.

Objective To observe the possibility and efficacy tolerability of topiramate(TPM) in transferring monotherapy for the patients with partial seizures with or without secondary general seizure,as well as to find out the optimum way of the transferring.Methods After TPM add on therapy 64 patients with seizures reduced ≥50%, it reduced about 1/4 of AEDs or one drug each time according to every two weeks until gradually to stop fully and transferring monotherapy.Results 18 patients were successful with TPM monotherapy (28.13%), 35 patients were half success(54.69%), 11 cases for failure (17.19%). The less AEDs combined, the higher possibility of transferring monotherapy would be. The rate of success for cabamazepine seemed higher than valproate.Conclusion It was possible to transfer monotherapy by TPM. The rate of success was 28.13%. It was easier to reduce cabamazepine than valproate. In the transferring monotherapy it was better to reduce one after another, in principle the one of the little dose was reduced first,and then the hepatic enzyme inducing agents was reduced. Even if the transferring was half success,the kinds of concomitant AEDs could be reduced at least, it was in line with the policy of medicine application.

Objective To observe the therapeutic efficacy and safety of Topiramate (TPM) as monotherapy in patients with partial epilepsy.Methods The seizure rate in baseline phase of 30 patients with partial epilepsy (treated by Topiramate as monotherapy for 20 weeks) were observed and recorded before treatment.The TPM starting dosage was 25 mg/d, and then increased 25 mg/d each week for 8 weeks until effective dosage or 200 mg/d for 12 weeks,the change of seizure frequency and bad reaction were found.Results The seizure in 16 cases (53.3%) was totally controlled,seizure reduction ≥75% was in 6 cases (20%),≥50% was in 2 cases (6.7%),

AIM: To study the protective effect and mechanism of melatonin on learning and memory in mice with repeated cerebral ischemia and reperfusion and its mechanism. METHODS: The models of repeated cerebral ischemia reperfusion were established in mice. The function of learning and memory was tested by the electric mare method, and the concentrations of MDA and NO were measured by colorimetric method and nitric reduction method in brain tissue. RESULTS: MT could obviously improve the function of learning and memory of model mice, inhibit cerebral dropsy, and lower the content of MDA and NO in cerebral tissue. CONCLUSION: MT shows significantly protective effect against repeated ischemia reperfusion injury in mice, and the mechanism might be related to inhibit the rise of MDA and NO in brain tissue.

Objective:To analysis the effects of alprostadil and yishen huashi particles on blood glucose,blood lipid,renal function and urinary podocyte proteins of patients with diabetic nephropathy.Methods:98 patients with diabetic nephropathy were selected and randomly divided into the control group and the experimental group with 49 cases in each group.The patients in the control group were treated with alprostadil,while the patients in the experimental group were treated with yishenhuashui particles on the basis of the control group.Then the curative effect,the levels of glycosylated hemoglobin (HbAlc),blood glucose (FPG),2 h postprandial blood glucose (2h PG),triglycerides (TG),total cholesterol (TC),high-density lipoprotein (HDL-C),low density lipoprotein (LDL-C),blood urea nitrogen (BUN),creatinine (Cr),β2 microglobulin (β2-MG),bladder inhibition (Cys-C) and urinary podocyte proteins (PCX) in the two groups were observed and compared between the two groups before and after the treatment.Results:The total effective rate of experimental group was higher than control group (P＜0.05).After treatment,there was no statistically significant difference about the HbA1c,FPG and 2 HPG between the two groups (P＞0.05).After the treatment,the levels ofTG,TC,LDL-C,BUN,Cr,β2 MG,Cys C,PCX and urinary nephrin/urine Cr of the experimental group were lower than those of the control group (P＜0.05).The HDL-C of experimental group was higher than that of the control group (P＜0.05).Conclusion:The curative is effect of alprostadil and yishen huashi particles in treatment diabetic nephropathy patients,can conducive to the improvement of blood glucose,blood lipid,renal function,reduce the concentration of urinary podocyte related proteins.

Objective: To explore the best method to treat post-stroke depression. Methods:60 Cases of the patients with post-stroke depression were selected and divided randomly into the two groups, 30 cases in each group. The treatment group was treated by scalp acupuncture plus body acupuncture, and the control group was treated by oral administration of Fluoxetine. The therapeutic effects were assessed after 30 d of the treatments. Results: The total effective rate was 90.0% in the treatment group, with average effective time of 5 d, and was 80.0% in the control group, with average effective time of 15 d. The comparison of the total effective rates between the two groups was not significant statistically (P＞0.05). But the comparison of the average effective time was significant statistically (P＜0.05). Conclusion: The therapeutic effect is positive in the treatment of post-stroke depression by scalp acupuncture plus body acupuncture, similar to the therapeutic effect of Fluoxetine, but faster in the effective time.

Objective To investigate the prevalence and the related factors of female breast disease in Zhengzhou City, Henan Province, and to provide a targeted prevention guide for female breast disease. Methods A total of 6310 women were enrolled in this study. In addition to breast ultrasound, mammography and pathology assays were performed. Finally, the prevalence and influencing factors for female breast disease were analyzed. Results The prevalence of breast cancer and the total prevalence of breast diseases was 0.06% and 24.94%, respectively. The prevalence of female breast diseases was significantly correlated to age, educational level, occupa-tion, menstruation, reproductive age and a history of abortion. Logistic regression analysis showed that the occupa-tional type had a significant effect on the prevalence of female breast. Conclusion The prevalence of female breast disease is relatively high in Zhengzhou City, and it is affected by many factors. The targeted surveys and breast dis-ease screening should be conducted, and the secondary prevention of female breast disease should be strengthened.

Objective To explore the expression and significance of COX-2 ,MMP-9 and PTEN in colonic adenocarcinoma tissues .Methods Seventy-two cases of pathologically archived paraffin specimens after colonic adenocarcinoma radical operation in the hospital from March 2014 to May 2016 were collected ,30 cases of colonic adenoma tissues and 30 cases of normal mucosal mem-brane tissues served as the controls .The expressions of COX-2 ,MMP-9 and PTEN were detected by adopting the immunohisto-chemical SP method .Then the correlation between COX-2 ,MMP-9 and PTEN with clinicopathological features of colonic adenocar-cinoma was analyzed .Results The positive expression rate of COX-2 and MMP-9 in colonic adenocarcinoma tissues was significant-ly higher than that in colonic adenoma tissues and normal colon mucosal membrane tissues (P<0 .05);the positive expression rate of PTEN was significantly lower than that in colonic adenoma tissues and normal colon mucosal membrane tissues (P<0 .05) .The expression of COX-2 ,MMP-9 and PTEN in colonic adenocarcinoma was correlated with the tumor invasive depth ,lymph node me-tastasis and TNM staging(P<0 .05) ,while had no correlation with sex ,age ,tumor morphology ,tumor size and differentiation de-gree(P>0 .05) .COX-2 ,MMP-9 and PTEN expressions showed negative correlation (r= -0 .260 ,-0 .282 ,P<0 .05) ,COX-2 and MMP-9 expression showed a positive correlation (r=0 .335 ,P=0 .004) .Conclusion The abnormal expression and interaction of COX-2 ,MMP-9 and PTEN are closely correlated with the occurrence ,invasion and metastasis of colonic adenocarcinoma .Their combined detection has an important significance for early diagnosis and prognosis assessment of colonic adenocarcinoma .

Objective To explore expressions and clinical significance of CDX2,COX-2 and VEGF in colon carcinoma tissues.Methods The expressions of CDX2,COX-2 and VEGF in colon carcinoma tissues of 62 cases and normal colon tissuesof 20 cases were detected by immunohistochemical SP methods.Relationships between clinical pathological features and CDX2,COX-2 andVEGF were analyzed.Results The positive expression rates of CDX2 in colon carcinoma tissues and normal colon tissues were 54.8％ and 95.0％ respectively,the positive expression rates of COX-2 in colon carcinoma tissues and normal colon tissues were 75.8％ and 20.0％,and the positive expression rates of VEGF in colon carcinoma tissues and normal colon tissues were 80.6％ and 25.0％.The positive expression rate of CDX2 in colon carcinoma tissues was significantly lower than that in normal colon tissues(P ＜ 0.05),the positive expression rates of COX-2 and VEGF in colon carcinoma tissues were significantly higher than those in normal colon tissues (P ＜ 0.05).The positive expressions of CDX2,COX-2 and VEGF in colon carcinoma tissues were related with invasive depth and lymph node metastasis (P ＜ 0.05).Spearman correlation analysis results showed that expression of CDX2 was negatively correlated with COX-2 and VEGF in colon carcinoma tissues (P ＜ 0.05),and COX-2 was positively correlated with VEGF in colon carcinoma tissues (P ＜ 0.05).Conclusion The expressions of CDX2,COX-2 and VEGF are closely related with the occurrence,development and prognosis of colon carcinoma.

Objective To explore expressions and clinical significance of CDX2,COX-2 and VEGF in colon carcinoma tissues.Methods The expressions of CDX2,COX-2 and VEGF in colon carcinoma tissues of 62 cases and normal colon tissuesof 20 cases were detected by immunohistochemical SP methods.Relationships between clinical pathological features and CDX2,COX-2 andVEGF were analyzed.Results The positive expression rates of CDX2 in colon carcinoma tissues and normal colon tissues were 54.8％ and 95.0％ respectively,the positive expression rates of COX-2 in colon carcinoma tissues and normal colon tissues were 75.8％ and 20.0％,and the positive expression rates of VEGF in colon carcinoma tissues and normal colon tissues were 80.6％ and 25.0％.The positive expression rate of CDX2 in colon carcinoma tissues was significantly lower than that in normal colon tissues(P ＜ 0.05),the positive expression rates of COX-2 and VEGF in colon carcinoma tissues were significantly higher than those in normal colon tissues (P ＜ 0.05).The positive expressions of CDX2,COX-2 and VEGF in colon carcinoma tissues were related with invasive depth and lymph node metastasis (P ＜ 0.05).Spearman correlation analysis results showed that expression of CDX2 was negatively correlated with COX-2 and VEGF in colon carcinoma tissues (P ＜ 0.05),and COX-2 was positively correlated with VEGF in colon carcinoma tissues (P ＜ 0.05).Conclusion The expressions of CDX2,COX-2 and VEGF are closely related with the occurrence,development and prognosis of colon carcinoma.