OBJECTIVE:To study the species and drug resistance of the bacteria isolated from sputum specimens of the inpatients in our hospital.METHODS:The drug susceptibility of 2 255 strains of pathogenic bacteria cultured from 6 052 qualified sputum specimens that collected from inpatients of our hospital between Jan.2005 and July 2007 were analyzed retrospectively.RESULTS:Of the 2 255 strains of pathogenic bacteria,1 210(53.7%)were gram negative bacillus,with Pseudomonas aeruginesa,Escherichia cob,Klebsiella pneumoniae and Acinetobacter showing the highest proportion,accounting for 19.5%,13.9%,8.2% and 6.4%,respectively;429(19.0%)were gram positive coccus,with CN-S and Staphylococcus aureus showing the highest proportion;and the 519(23.0%)were fungus with Candida albicans showing the highest proportion.CON-CLUSION:The result of drug susceptibility test showed that both gram negative bacilli and gram positive coccus were highly resistant to quite a few of antibiotics.

Objective To explore the prevention and treatment principles of different types of iodine contrast agents.Methods 240 patients with CT enhancement were divided into two groups according to the contrast agent type: 80 patients with ionic contrast agent (control group), 160 cases with non-ionic contrast agent (observation group).The allergic reaction was evaluated according to the ADR classification.The constituent ratio of the allergic reaction was observed in the two groups.The contrast ratio of the hypersensitive reaction was observed.The clinical manifestations of the allergic reaction were observed.Results In the control group, there were 8 cases of mild allergic patients,7 cases of mild allergic patients in the observation group,7 cases of moderate allergic patients in the control group,6 cases of moderate allergic patients in the observation group,4 cases of severe allergic patients.There were 2 cases of severe allergic patients in the observation group.In the observation group, there were 15 cases of total allergies and 19 cases of total allergies in the control group.The number of allergies in the observation group was lower than that in the control group (x2=9.063,P<0.05).80 cases of contrast agent induced 19 cases of allergic reaction;non-ionic contrast agent iodopromide 79 cases, induced 9 cases of allergic reactions;non-ionic contrast agent iodohydrin contrast 81 cases.Allergy symptoms such as skin rash, malaise, chills and fever, eyelid edema, throat discomfort and voice, such as nausea and vomiting, dizziness, chest tightness, cardiovascular symptoms such as shock, blood pressure changes and so on.Conclusion Non-ionic iodine contrast agent is superior to ionic contrast agent in the incidence of allergic reaction.However, it is an important measure to reduce the patients' injury caused by different types of hypersensitivity reaction.It is worth the attention of clinicians.

OBJECTIVE:To evaluate the efficacy of esomeprazole vs.lansoprazole in the treatment of patients with reflux esophagitis (RE).METHODS:A total of 120 patients with RE were randomly assigned to receive either esomeprazole (20 mg n&m,treatment group) or lansoprazole (30 mg n&m,control group) for 8 weeks.The symptomatic scores including heartburn,retrosternal pain and acid regurgitation,and gastroscopic curative efficacy at 4 and 8 weeks were obtained for the evaluation of clinical efficacy.RESULTS:In the treatment group at 4 and 8 weeks after treatment,the total effective rates in improvement of clinical symptoms were 78.1% and 87.5%,respectively,and the total effective rates of gastroscopic curative efficacy were 87.5% and 93.8%,respectively;while in the control group at 4 and 8 weeks,the total effective rates in improvement of clinical symptoms were 60.7% and 73.2%,respectively,and the total effective rates in gastroscopic curative efficacy were 73.2% and 80.4%,respectively,showing statistical differences in total effective rates between esomeprazole and lansoprazole(P

OBJECTIVE: To investigate the protective effects of tea polyphenol (TP) on lung injury caused by global cerebral ischemic-reperfusion and its mechanism. METHODS: Improved Pulsinelli-brierley method was applied to induce damage model by global cerebral ischemia model for 15 min and global cerebral ischemia-perfusion for 3 h. Some indexes were observed, including morphology change of cerebral and lung tissue, activity of superoxide dismutase (SOD), concentration of malondialdehyde (MDA) and expression of lipopolysaccharide receptor CD14. Healthy rats were divided into sham group, model group and nimodipine group. RESULTS: As compared with sham group, there were pathologic damage of lung and brain and the positive expression of CD14 cell of lung in model group. As compared with model group, the activity of SOD were increased significantly (P

Objective:To investigate the clinicopathological significance of the CyclinB1 and CDK1 expression in stomach carcinogenesis.Methods:The expression of CyclinB1 and CDK1 was examined by immunohistochemistry method in normal epithelium(15 cases),low-differentiated adenocarcinoma (15 cases)and moderate-well-differentiated adenocarcinoma (20 cases).Results:Immunoreaction to Cyclin B1 and CDK1 was mainly localized in the cytoplasm of cells.In stomach carcinogenesis,the positive immunostaining rate of CyclinB1 protein in normal epithelium and adenocarcinoma was 13.3%(2/15),94.3%(33/35) respectively (P

150 eyes (85 patients) of angle closure glaucoma treated with Nd: YAG laser iridectomy were analyzed to reveal that the basic factors leading to posterior synechiae were the dosage and duration of miotics used. In the cases with posterior synechiae, miotics of higher dosages were used before and after the laser iridectomy and also a longer duration before the operation than in cases without the sequelae. With posterior synechiae, the pathological damages to the optic nerve worsened with time to affect the visual acuity and the control of IOP, and also interfered with fundus examination and cataract extraction when needed. The study indicated that proper dosage of laser energy,intensive postoperative use of topical steroids, early dilatation of the pupil, and appropriate application of mioties were conducive to the prevention of posterior synechiae.

ObjectiveTo explore the therapeutic effect of acute non-lymphocytic leukemia(ANLL) with pathological karyotypes.MethodsAnalyze karyotype with the R-banding technique.The experimental group contained 111 ANLL patients with pathological karyotypes and the control group contained 69 ANLL patients with normal karyotypes.ResultsThe complete remission (CR) rate was apparently lower in the group of pathological karyotypes than that in the group of normal karyotypes (44.14% vs 65.22%;P

Objective: To explore the inhibitory effect of pharmacological ligand to PPAR?-rosiglitazone on smooth muscle cell proliferation of rat aorta.Methods: smooth muscle cells were prepared from rat aortas using explant technique and identified by their specific growth characteristic and immunocytochemistry.The fifth passage cells were made quiescent by serum starvation for 24 hours,and then cells were stimulated with PDGF-BB or bFGF(20ng/ml) for 24 hours in the presence or absence of rosiglitazone(1~10umol/L),pretreated for 30 minutes.MTT assay was applied to assess cellular viability.Flow cytometry was used for cell cycle analysis.Results: Under microscope,the cultured cells possessed "peak and valley" characteristics for smooth muscle cell.Immunocytochemical staining with specific mAb against ?-actin demonstrated these cells were positive.Smooth muscle cell proliferation was induced by PDGF-BB or bFGF with an increase A490 in MTT.The cell number was reduced in the S phase and increased in the G0/G1 phase.Cell proliferation and transition from G0/G1 to S were inhibited using pretreatment of rosiglitazone in a dose-dependent manner.Conclusion: Rosiglitazone has inhibitory influence on vascular smooth muscle cell proliferation induced by growth factors.

Objective:To explore the effect of angelica polysaccharide(APS)on adhesion molecules expressions in murine hematopoietic cells,in order to provide experimental evidence of APS on hematopoietic regulation and the mechanism of mobilizing.Methods:Techniques of peripheral blood white blood cell(WBC)and bone marrow mononuclear cells(MNC)count were made.Flow cytometry detected the rate of Sca-1~+ cells in peripheral blood and bone marrow and the expression of CD49d (VLA-4 of a chain),CD44 on bone marrow MNC and Sca-1~+ cells.Results:1.In APS group(4mg/kg):the number of peripheral blood WBC and Sca-1~+ cells were markedly higher than that of NS group(P

Objective:To study the biologic characters of human foreskin fibroblasts(HFF)to lay the foundation for a long-term culture for human embryonic germ cells in vitro.Methods:The method of separating HFF was investigated.By immunocytochemical method,MTT and flow cytometry,we investigated the morphology and the purity of HFF,study the cell growth curve and cell cycle of HFF,and the effects of different concentrations of mitomycin on growth of HFF at different times.Results:Epidermis and dermis were more easily separated after overnight digestion(12~16 h)in 0.25% Trypsin at 4℃,compared with incubation for 2 h in 5% CO2 at 37℃.Vimentin was strongly positive stained with the purity of 95 percent.There was no obvious stranded period.The rapid proliferation period was in 1~6 d of cell growth curve.The platform stage began at 7 d.Majority of the cells were in G1/G0 phases.The cell cycle corresponded to cell growth curve.Mitomycin C(MMC)inhibited the proliferation of HFF at 12.5 ?g/ml over 2.5 h of MMC treatment.Conclusion:Its biological characters showed that HFF can be used as feeder layer for in-vitro cultured human embryonic germ cells。