The constitutional medical thoughts in Inner Canon of Huangdi is the foundation for TCM constitution theory, it discusses the influence of constitution on disease in detail. Constitution factors determine the disease onset or not; Constitution factors determine the tendency and character of disease. Constitution factors effect the changes of pathogenesis, the character of syndrome, the therapeutic principle and methods; Constitution factors effect the turnover and prognosis. Constitution therapies are profit to correct the partial body, preventing the development of disease and heal the disease.

Parkinson’ s disease ( PD) is a neurodegenerative disorder primarily characterized by resting tremor, muscular rigidity, akinesia and postural reflex impairment.Behavioral tests of PD in animal models are essential for under-standing the pathogenesis of PD as well as for the development and testing of potential therapeutics .Here we mainly use the 6-hydroxydopamine (6-OHDA)-induced rat model, to introduce a review on the research progress in non-drug-induced be-havioral tests of motor function in PD rats.

Proteomic research first came to the fore with the introduction of two-dimensional gel electrophoresis and proteomics has been increasingly applied to cancer research with the wide-spread introduction of mass spectrometry and protein chip.As important research tools,oncoproteomics that targets the entire cancer-specific protein network has already been applied in cancer research.In this article the development of the oncoproteomics and its applying in cancer is reviewed.

Objective To investigate the changes of autoantibodies in hepatitis B disease and its clinical sig-nificance.Methods 418 cases of hepatitis B patients were selected as hepatitis B group.At the same time,148 hos-pitalized patients in our hospital with autoimmune hepatitis( AIH) were chosen as AIH group.And 100 healthy people with physical examination were selected as control group at the same time .The antinuclear antibodies , anti -smooth muscle antibody( SMA) and anti-kidney microsomal antibodies and other autoantibodies of the three groups were detected and analyzed statistically.Results ANA-positive rate of hepatitis B group was 22.73%,ANA titer was mainly 1:100,only two cases of liver cirrhosis titers were 1:320,ANA karyotype was mainly homogenous,only four cases belonged speckled pattern;ANA positive rate of AIH patients was 60.14%,ANA titer was mainly 1:320,or even 1:1 000, primarily as a spot -type karyotype, nucleolar and centromere type.Differences between the two groups were statistically significant (P<0.05).The positive rates of hepatitis B,chronic hepatitis,severe hepatitis B and hepatitis B patients with cirrhosis ANA were significantly higher than in patients with acute hepatitis B,the differ-ences were statistically significant (χ2 =12.172,12.560,28.123,all P<0.05).Compared with AIH group,the posi-tive rate of SMA and LKM in patients with hepatitis decreased 51.98%,32.22%,respectively,the differences were statistically significant (χ2 =196.843,107.357,all P<0.05).The positive rate of hepatitis B level ANA ( +) patients of ALT and AST,HBV DNA was significantly higher than ANA (-) group.The level of hepatitis B patients ALT and AST,HBV DNA positive group were significantly higher than AIH.Differences were statistically significant (all P<0.05).Conclusion Autoantibody detection helps to study autoimmune etiology and pathogenesis of great importance for patients with hepatitis B and cirrhosis,but the reaction involved in the pathogenesis of autoimmune hepatitis level research still needs to be deeper.

The effects of spindle afferents activated by stretching gastrocnemius on the nociceptive responses of WDR neurons were recorded extracellularly with microelectrode and observed in paralyzed rats. The results indicated that in 51/80, the spindle afferent activity activated by stretching can inhibit nociceptive responses of neurons. 16 out of 80 neurons no effects can be observed, in 13/80 the effects of spindle afferent activity can excite the nociceptive responses of WDR neurons. Among 51 units, which have inhibitory effects induced by stretching muscle, the inhibitory effects induced by instance and bilateral stretching are stronger than that induced by continuous and unilateral stretching. These results suggested that spindle afferent activity is possesaed of analgesic effects.

Objective To investigate the effects of sulfur dioxide(SO2) pollution in different concentrations on activity of SOD,levels of GSH and MDA in heart and lung tissue of exercised rats,in order to elucidate the toxicological mechanism of SO2 pollution.Methods Forty-eight SD rats were randomly divided into eight groups:a rest group(RG),an exercise group(EG),a low pollution exercise group(LEG),a low pollution rest group(LRG),a moderate pollution exercise group(MEG),a moderate pollution rest group(MRG),a high pollution exercise group(HEG) and a high pollution rest group(HRG).All groups,except RG and EG,were exposed to SO2 with different concentrations(5 mg/m3,10 mg/m3,15 mg/m3),meanwhile exercised rats run in a motor-driven wheel at a speed of 8 m/min,2 h/d for 10 days.Rats were sacrificed at 24 h after treatment and the anti-oxidative enzymes activity and lipid peroxidation(LPO) levels were measured.Results Compared with RG,SOD activity of lung tissue significantly decreased in HEG,MEG,LEG(P

We have observed the effects of five alga polysaccharides on rabbit's blood platelets aggregation. We found that the polysaccharides could inhibit aggregation of blood platelets in vitro. The strongest effect was observed in the polysaccharides from the following,in decreasing order of strength: Scytosiphon lomentarius, Codium fragile,Laminaria japonica and Ulvalactuca. The same effect was not observed in polysaccharides from Cladophra. We also found a positive correlation between the effects of anti-aggregation and the ratio of vitriol radicles in these five polysaccharides.

Blood pressure (BP) is one of important physiological parameters which reflect the activity of cardiovascular system. The discriminant method of BP is the key to improve the accuracy of measurements. In this paper, the principle of oscillation method is introduced first. The factors that influence the accuracy of this method and the preprocessing of pulse wave are also analyzed. Then, an improvement method based on oscillation method is proposed by means of the flow progress diagram, which is applied to measuring BP simulator and human body. The measurements are compared with the standard values of BP simulator and the measurements by Korotkoff sound method respectively. The results validate that the accuracy and the repeatability of BP determination are improved dramatically.

This paper describes the measuring principle and algorithm of atherogenic index with oscillometric model. The algorithm proves effective and reliable through analyses of many cases and lots of clinical tests, which has been applied to the development of Lab-VIEW-based software for clinical use. Both patients and doctors can turn to this software for the information of angiosclerosis.

Objective To study the characteristic of operation design and analyze curative effect of laser sub-epithelial keratomileusis (LASEK) on low and midrange myopia in aged patients. Method Fiftyeight patients ( 114 eyes) no less than 35 years old were treated with LASEK by monoblepsis. The subjects were examined and analyzed at 3 days, 7 days, 1 month,3 months and 6 months after operation. Results Seventy-one corneas(62.28%) recovered well at 3 days after operation. At 1 month after operation, the acuity of vision of all patients achieved or exceeded corrected vision before operation,and lasted to 6 months after operation. The break up time of 63 eyes were lower than normal at 3 months after operation,but 6 months after operation all of them recovered to normal. The satisfaction of patients raised gradually. Conclusions LASEK is effective for no less than 35 years old myopic patients. Make operation plan refer to monoblepsis and patients' needs can receive more satisfaction.