150 eyes (85 patients) of angle closure glaucoma treated with Nd: YAG laser iridectomy were analyzed to reveal that the basic factors leading to posterior synechiae were the dosage and duration of miotics used. In the cases with posterior synechiae, miotics of higher dosages were used before and after the laser iridectomy and also a longer duration before the operation than in cases without the sequelae. With posterior synechiae, the pathological damages to the optic nerve worsened with time to affect the visual acuity and the control of IOP, and also interfered with fundus examination and cataract extraction when needed. The study indicated that proper dosage of laser energy,intensive postoperative use of topical steroids, early dilatation of the pupil, and appropriate application of mioties were conducive to the prevention of posterior synechiae.

Objective To express dengue virus(DENV)NS2B-NS3 protease in E.coli,optimize the expression conditions and determine the enzyme activity,so as to lay a foundation of screening and discovering of lead compounds targeting DENV.Methods Codon-optimized NS2B-NS3 gene was inserted into pET-28a vector to construct recombinant prokaryotic expression plasmid pET-28a-NS2B-NS3,which was transformed E.coli Rosetta(DE3)competent cells and induced by IPTG to express NS2B-NS3 protease. The optimal expression conditions of NS2B-NS3 protease in E.coli were determined by optimizing induction length,induction temperature and IPTG concentration. NS2B-NS3 protease was isolated and purified by HisTrap~(TM) affinity chromatography column and measured for the protease activity by fluorescence resonance energy transfer(FRET)assay.Results The recombinant prokaryotic expression plasmid pET-28a-NS2B-NS3 was constructed correctly as identified by restriction analysis(NheⅠ/XhoⅠ)and sequencing. The optimal expression conditions of NS2BNS3 protease in E.coli were as follows:induction temperature of 20 ℃,induction length of 10 h and IPTG concentration of0. 2 mmol/L. The purified NS2B-NS3 protease showed a purity of more than 90% with a exhibited a of 20 mg/L,which bound to mouse monoclonal antibody against His-tag specifically and had good hydrolytic activity with a specific activity of 16. 111 U/mg,a K_m of 16. 46 μmol/L and a k_(cat) of 0. 028/s.Conclusion DENV NS2B-NS3 protease with high purity and activity was successfully prepared,which laid an experimental foundation of the establishment of high-throughput screening model for inhibitors targeting NS2B-NS3 protease.

Objective To investigate medical and nursing interns' differences and similarities of role expectation on clinical nurses,and provide the basis for further carrying out physician-nurse cooperative education.Methods Using self-designed questionnaires of role expectation on clinical nurses to investigate medical and nursing interns of two degrees in Zhengzhou University who already had full ten months' internship,among them,154 medical students,145 nursing students.Results Medical and nursing students' differences were mainly on “physician-nurse collaboration and communication.Nursing students were more active than medical students.Both medical and nursing students had the lowest scores in item 15 (reverse entry)“The nurses just need to perform doctors' orders,without providing comments or suggestions”.Conclusions Colleges and clinical teachers should encourage nursing students to learn more medical knowledge,strengthen their clinical practice capacity,improve their professional competence and professionalism,thereby enhancing the status of nursing and nurses.Launching physician-nurse collaboration education in nursing and medical students,and help medical and nursing students realize nurses' different roles,improve the trust between doctors and nurses,promote the development of physician-nurse relationship.

4.0 mg/L) fluorosis areas in 2008.The fluoride content of the drinking water,the chlidren's dental fluorosis and urinary fluoride were investigated.Results The fluoride in drinking water in the water improved areas was(1.4?0.8)mg/L,which was significantly lower than that in the non-water improved areas(2.6?1.5)mg/L(P

Objective: To explore the Asian Resilience Scale’s preliminary revision, reliability and validity in college students of China. Methods: 646 students were investigated with the Asian Resilience Scale and the short form of NEO Five-Factor Inventory. Results: ①Three factors were extracted by exploratory factor analysis and explained 47.1% of variance. ②Confirmatory factor analysis showed good fit between the data and the construct of scale, ?2/df=2.36,RMSEA= 0.055,GFI=0.923,CFI=0.908,NNFI (TLI)=0.894. ③The Cronbach’s ? coefficients, split-half and retest reliability of the total scores were 0.865, 0.836 and 0.894 respectively. ④The total score of the Asian Resilience Scale was significantly negatively correlated with neuroticism and positively correlated with extraversion, openness to experience, agreeableness and conscientiousness. Conclusion: Reliability and validity of the Asian Resilience Scale’s revised Chinese version is corresponded with psychometric standard, and it could be used to assess the resilience of Chinese college students.

Objective To investigate the level of urinary TGF-β1 and evaluate its clinical significance in patients with chronic nephritis.Methods 22 healthy people and 40 patients with chronic nephritis were selected,the levels of urinary TGF-β1 were measured by RT-PCR and ELISA.Results Urinary TGF-β1 were significantly increased in Mesangial Proliferative Glomerulonephritis (76.44± 24.29 ng/mmol.Cr vs 31.53 ± 11.59 ng/mmol.Cr,P<0.01);Focal Glomerular Sclerosis (65.07±20.59 ng/mmol.Cr vs 31.53±11.59 ng/mmol.Cr,P<0.01)and IgA Nephritis (67.01±21.95 ng/mmol.Cr vs 31.53±11.59 ng/mmol.Cr,P<0.01),comparing with normal group.Conclusion The levels of urinary TGF-β1 could be a predictor in reflecting the sate of renal diease,estimating pathological type,diagnosing the renal diease and observing the therapeutic effects.

Parkinson’ s disease ( PD) is a neurodegenerative disorder primarily characterized by resting tremor, muscular rigidity, akinesia and postural reflex impairment.Behavioral tests of PD in animal models are essential for under-standing the pathogenesis of PD as well as for the development and testing of potential therapeutics .Here we mainly use the 6-hydroxydopamine (6-OHDA)-induced rat model, to introduce a review on the research progress in non-drug-induced be-havioral tests of motor function in PD rats.

Carcinoma of unknown primary (CUP) is a diagnostic and therapeutic challenge in oncology.The early and correct detection of the primary tumors of CUP is very important to its diagnosis, treatment, therapeutic response evaluation and prognosis prediction.This review summarizes the implications and development of the imaging technologies, especially 18F-FDG PET/CT scan, in the diagnosis, treatment and prognosis prediction of CUP patients.

Objective:To evaluate the periodontal tissue regeneration effect of implantation of bone marrow stromal cells(BMSCs)-root-coralline hydroxyapatite(CHA) complex.Methods:BMSCs of beagle dogs were in vitro cultured on CHA scaffold and on tooth root surface respectively.Then BMSCs-root samples were inserted into BMSCs-CHA scaffolds to form BMSCs-CHA-root complexes.5 complexes were implanted on one side of mandible in each of the dogs 1 month after the 4th,5th and 6th teeth had been extracted.Root-CHA without BMSCs was implanted on another side as the control.All dogs were killed 16 weeks after implantation,and the periodontal tissue regeneration were observed histologically.Results:More new bone formation and more mature alveolar bone were observed in the test group than that in the control,and there was a clearly periodontal ligament-like tissue formation only in the test group.Conclusion:The complex of BMSC-root-CHA can improve the periodontal tissue regeneration.

Objective To explore a simple, effective and rapid method for indoor air disinfection. Methods Three kinds of disinfection methods were carried out (method A: wiping surfaces of floor and walls of room with water and ultraviolet irradiation, method B: wiping surfaces of floor and walls with chloro-disinfectant and ultraviolet irradiation, method C: wiping surfaces with chloro-disinfectant and spraying chloro disinfectant containing chlorine) and the effectiveness of different disinfection methods was compared. Results The result showed that all of the three methods could decrease natural fallen bacteria count in isolated room air to up to the standard, and the disinfection effectiveness of method C was better than the method A and method B. Conclusion The result indicated that method C was an effective and simple method for indoor air disinfection.