Objective:To explore therapeutic effect of levosimendan on refractory heart failure (RHF).Methods:A total of 60 patients,who were diagnosed as RHF in our department of cardiology from Sep 2010 to Sep 2014,were selected.Ac- cording to random number table,they were randomly and equally divided into routine treatment group and levosimendan group (received levosimendan based on routine treatment group).Left ventricular ejection fraction (LVEF),plasma level of brain natriuretic peptide (BNP)before and after treatment,6min walking distance (6MWD)on 60d after treatment and total effective rate were compared between two groups.Results:Total effective rate of levosimendan group was significantly higher than that of routine treatment group (90.0% vs.76.7%,P=0.03).Compared with routine treatment group af- ter treatment,there were significant rise in LVEF [(41.53±3.42)% vs.(48.84±3.57)%]and 6MWD [(371.0±69.5) m vs.(467.2±87.3)m],and significant reduction in plasma BNP level [(453±39)pg/ml vs.(199±27)pg/ml]in levo- simendan group,P<0.05 all.Conclusion:Levosimendan possesses significant therapeutic effect on refractory heart failure without severe adverse reaction,which is worth extending.

Objective To explore the change of 25 hydroxyvitamin D level and its correlation with glucose metabolism and bone mineral density in elderly men with type 2 diabetes mellitus(T2DM).Methods Hospitalized 53 elderly male patients with T2DM (T2DM group) and 51 patients without T2DM (NDM group) were chosen.Blood.biochemical indexes and 25 hydroxyvitamin D level were measured.Bone mineral density of lumbar (L2_4),left proximal femur was measured by dual energy X-ray.Results The serum 25 hydroxyvitamin D level in T2DM group was lower than that in NDM group [(12.38 ±5.12)μg/L vs.(17.35 ± 5.52) μ g/L],and there was significant difference(P＜ 0.05).The serum 25 hydroxyvitamin D level was negatively associated with fasting blood glucose level and glycosylated hemoglobin (r =-0.225,-0.499,P ＜ 0.05).The serum 25 hydroxyvitamin D level was not associated with fasting insulin and homeostasis model insulin resistance index (r =-0.050,-0.082,P ＞ 0.05).The incidence of osteoporosis in T2DM group was higher than that in NDM group [30.19％(16/53) vs.11.76％(6/51)],and there was significant difference (P ＜0.05).The serum 25 hydroxyvitamin D level was positively associated with bone mineral density of femur neck,greater trochanter of femur and Ward triangle(r =0.773,0.762,0.812,P ＜ 0.05).The serum 25 hydroxyvitamin D level was not associated with bone mineral density of lumbar (L2_4)(r =0.116,P ＞ 0.05).Conclusions The prevalence of vitamin D deficiency is found in elderly men with T2DM.Vitamin D level can affect blood glucose control and increase the risk of the occurrence of osteoporosis.Therefore,T2DM patients,especially elderly patients,should be routine vitamin D testing.Vitamin D supplementation to the patients of vitamin D insufficiency in time may be beneficial to glucose metabohsm and the prevention and treatment of osteoporosis.

Objective To explore the effect of dinoprostone suppositories and oxytocin on cervical maturation and delivery outcome in induction of labor in late pregnancy.Methods A total of 174 pregnant women in the department of obstetrics and gynecology in our hospital for treatment in late pregnancy were chosen.They were randomly divided into two groups: the control group 85 cases and the study group 89 cases.The control group was treated with routine induction of intravenous infusion of oxytocin, and the study group was taken with dinoprostone suppositories.The effective rates of induction of labor and the incidence of adverse reactions after induction of labor induced of the two groups were observed.The clinical effect of patients was with Bishop score to determine cervical maturity.The induction of labor time, natural childbirth rate, cesarean section rate, maternal postpartum hemorrhage and neonatal asphyxia score were as the evaluation criteria of childbirth outcome.Results The cervical maturation level was (7.26 ±1.82) after drug for 24 hours in the study group, significantly higher than that of the control group (6.34 ±1.37) (P<0.05).The induction time, spontaneous delivery rate and cesarean section rate of the study group were (6.34 ±1.37)h, 86.52%, 13.48%, significantly better than those in the control group (P<0.05).The average amount of bleeding in the study group was (225.96 ±12.81) mL and the neonatal asphyxia score was (9.73 ±0.15), the results were lower than the control group (P<0.05).The successful rate of induction of labor in the study group was 96.63%) higher than that in the control group 85.88%.The incidence of side effects in the study group was 10.11%, significantly lower than in the control group 21.18% (P<0.05), which were with statistical significance.Conclusion Dinoprostosterone suppositories have the advantage of promoting cervical ripening for late pregnancy in pregnant women compared with oxytocin.It can shorten induction time and improve natural childbirth rate.And the maternal prognosis is good, can be used as a safe and effective clinical induction.

Intestinal fibrosis is the complications of inflammatory bowel disease (IBD), intestinal myofibro-blast cells are the key to intestinal fibrosis .Intestinal myofibroblasts and its interaction with inflammatory cells play an im-portant role in IBD-related intestinal fibrosis .

Integrins are the main mediators of the complicated interaction between hepatic stellate cells (HSCs) and extracellular matrix (ECM) components. Signal transduction by integrins is initiated by both occupancy and crosslinking of integrins by ECM components. Focal adhesion kinase (FAK) and mitogen- activated protein kinase (MAPK), which are the downstream molecules, involve in integrin-mediated cellular behaviors. In this review, we briefly summarize the integrin's structure, intracellular signaling cascades and its role in HSC behaviors,such as activation,proliferation and apoptosis.

RNA interference(RNAi),the first method of choice to suppress gene expression in vitro,is a conserved biologic response to double-stranded RNA that resulted in the sequence-specific silencing of target gene expression.Recently,more than 100 literatures reported synthetic small interfering RNAs in mammals.These reports demonstrated that RNAi is also a powerful tool for in vivo research and may become an effective therapeutic tool.This paper reviews the influencing factors and experimental design of in vivo use of RNAi.

Focal adhesion kinase(FAK),a non-receptor protein tyrosine kinase,is siting at the cross road of numerous intra-cellular signal transduction.The activated FAK has been implicated in a diverse array of cellular behavious.Recent research on FAK and fibrosis show that FAK plays an important role in fibrosis disease,so the targeting of FAK may be a novel therapeutic strategy.

Objective To investigate the correlation between NAD(P)H oxidase p22phox C242T polymorphism and carotid atherosclerosis(CA)in Chinese population.Methods 138 patients with CA and 130 control subjects were recruited.C242T polymorphism of p22 phox was analyzed by polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP)for determination of its genotype. Results The CT genotype frequencies in the CA group and control group were 0.268 and 0.139, respectively,and no TF genotype was found.The CT genotype frequency of the CA group was significantly higher than that of the control group(X2=6.899,P=0.009).It was found that systolic blood pressure, blood glucose,low density lipoprotein cholesterol smoking and the NAD(P)H oxidase p22 phox C242T polymorphism were independent risk factors for CA by Logistic regression analysis.Conclusions The NAD(P)H oxidase p22 phox C242T polymorphism may be a risk factor for CA.

The idea and methods of study on proteomics have many similarities to the whole view and the dialectical view of TCM,so it confirms with own characteristics of syndromes to introduce proteomics into studies of essence of syndromes.Appling the idea and methods of proteomics to study TCM differentiation of syndromes of hepatic disease and medicines for treatment and prevention of hepatic disease will comprehensively reveal the essence of generation,development and transformation of TCM hepatic disease,providing the most essential theoretical basis for TCM prevention and treatment of hepatic disease.To establish transform mode concept of correlation of proteomics with TCM hepatic disease will enrich greatly theory of TCM treatment of hepatic disease based on differentiation of syndromes,and also will provide a new thinking model for study on TCM syndrome types,and accelerate the process of TCM modernization.

Objective To study the RI value in HA to ocular blood flow in posthepatitis cirrhosis by CDFI.Methods The RI value of HA,OA,PCA,CRA in 32 normal person,102 posthepatitis cirhosis patients were studied by CDFI(U.S.A.Sequoia 512).Results The value of RI between HA to OA,PCA and CRA was 0.88,0.88,0.93 respectively.Conclusion A direct relationship is found between RI value of HA to ocular vascular,especially in HA to CRA.