The epidemic characteristics of differentiated thyroid cancer(DTC)are changing, thyrotropin(TSH)suppressive therapy is also improving in recent years. The risks of recurrence and the death of patients, as well as the adverse effects of levothyroxine sodium treatment should be fully considered during thyroid hormone administration for patients of DTC. Hence, the degree of TSH suppression should be individualized in patients with DTC.

T),resulting in His to Leu(CAT→CTT) substituted at codon 435(H435L).No mutation was identified in the patient's parents.HT occurred in the patient during following-up.Conclusion H435L mutation in exon 10 of TR? gene leads to resistance to thyroid hormone.Meanwhile,consistent RTH might lead to HT.

Objective:To synthesize substituted pyridine propynyl carbamates and to test their antimicrobial activities. Methods: Eight novel compounds were designed and synthesized. Antimicrobial tests in vitro were carried out with 8 common mildews (Aspergullus niger, Aspergillus flavus, Aspergillus versicolor, Trichoderma viride, Paecilomium varioti Bainier, Chaetomium globsum, Penicillium citrinum, Cladochytrium clodospoium) and 5 bacteria (Escherichia coli, Pseudomonas fluorescens, Bacillus fluorescens, Bacillus megatherium). Results: All compounds synthesized showed antimicrobial activity, especially the compound 1f, whose activity was more potent than that of compound 3-iodo-2-propynyl-butyl-carbamate (IPBC). Conclusion: Compound 1f is worth further studying and exploration.

Objective To investigate the effects of NE on hepatic stellate cells(HSCs)apoptosis.Methods After NE and the antagonists of adrenoceptor subtypes were administered to HSCs,MTT method was used to evaluate cell proliferation.TUNEL assay and flow cytometry(FCM)were used to identify cell apoptosis.Results(1)Compared with control group,NE at concentrations of 1、10 and 100 ?mol/L induced HSCs proliferation in a time-dependent manner;The action of NE at concentrations of 10 ?mol/L reached the peak(0.262?0.085 vs 0.048?0.055,P

Object To determine the amount of cichoric acid in Enchinacea purpurea and its preparation. Methods By the use of HPLC, mobile phase: 400 mmol/L ammonium acetate methanol (95∶ 5), flow rate: 1 mL/min, detection wavelength: 330 nm. Results The mean recovery of cichoric acid was 99 41%, RSD=3 23% (n=5). Conclusion The method can be applied in the determination of cichoric acid in E. purpurea extract and its preparation with satisfactory results.

Object To establish an isolation method of theanine reference substance by preparative HPLC. Methods After raw material extracted by water, the water phase was extracted by chloroform saturated with water, then concentrated on a thermostat. After centrifuged, supernatant was isolated and purified by preparative HPLC. Fraction was frozen and dried by Flexi Drier. Crude product was rinsed by methanol. The purity of product was determined by analytical HPLC. Results The purity of product is higher than 98% and theanine yield from raw material by this method exceeds 60%. Conclusion The developed method is simple, rapid, and at low production cost. The product owns the quality of reference substance.

Objective To investigate the expression of TLR2 in colon mucosa of ulcerative colitis (UC) and to analyze the correlation with clinical activity and endoscopic grading. Methods The biopsies from 47 UC patients and 13 healthy controls were collected, and the expression of TLR2 protein and mRNA in colonic mucosa was determined by Western Blot and semi-quantitative RT-PCR, respectively. The patients were graded according to endoscopic and clinical findings. Results The expressions of TLR2 protein and TLR2 mRNA in UC patients were significantly increased than those in healthy controls, which was correlated with the progression of the disease. Conclusion The expressions of TLR2 protein and TLR2 mRNA in colon mucosa from UC patients might be used as a marker for disease activity.

Objective To explore the relationship between snoring and hypertension in the elderly.Methods A total of 375 subjects who were ≥40 years without stroke and myocardial infarction accepted questionnaire survey on snoring.Non conditional Logistic regression analysis was used to analyze the factors of hypertension.Results In 375 subjects,non-snore was in 169 subjects,occasionally snore was in 86 subjects and regular snore was in 120 subjects,the prevalence of hypertension in them were 33.7％(57/169),39.5％(34/86) and 49.2％ (59/120),respectively,there was significant difference among them.Non conditional Logistic regression analysis showed:after correcting other factors,regular snore was still risk factor of hypertension,the OR value was 2.124 (95％ CI 1.189-3.796).According to gender stratification,regular snore was still risk factor of hypertension in female,the OR value was 3.993 (95％ CI 1.259-12.663),rather than risk factor in male.Condusion Regular snore is relevant to the increased prevalence of hypertension,and it is a risk factor of hypertension.

matK is one of the core DNA barcode markers for plant DNA barcode identification and its universality using single makers has been in controversy. However, the universalities of different matK primer pairs in same seed plant group (order) and same matK primer pairs in different seed plant groups (order) are lack of systematic research. In this study, we collected 14563 full-length matK sequences of 11429 species of 3292 genera in 239 families belonging to 36 orders in seed plants. The universalities of 13 matK primer pairs and its 78 primer com-binations have been assessed using bioinformatics methods. The results indicated that xf/5r, 1F/8R, 390F/1326R and 3F_KIM/1R_KIM were the four most universality primer pairs. The four markers' universalities were 91.18%, 84.65%, 79.81% and 80.94% respectively in all 11429 seed plants. The most universality primer pairs in different orders were different. For each order, the primer pair with maximum universality was different. the xf/5r was the basal primer pair for primer combination and 1F/8R, 1F/1R, M3/M4 and 3F_KIM/1R_KIM could be the complementary primer pairs. This study could be a valuable resource for the primer selection of the research DNA barcoding identification in seed plants.

Objective To identify highly sensitive and specific antigens in Aeromonas hydrophila strain ATCC7966 by using immunoproteomics.Methods Outer membrane proteins were extracted from the Aeromonas hydrophila strain by using two-dimension electrophoresis and identified by LC-LTQ-XL-MS ( liq-uid chromatography coupled with linear ion trap mass spectrometry).Western blot assay was performed to screen out the immunogenic proteins.Results A total of 43 peptides representing 39 proteins were identi-fied by LC-LTQ-XL-MS.Among the 39 proteins, 69% were outer membrane proteins and 12% were inner membrane proteins.They were involved in the process of transportation, cell motility, biosynthesis, etc. One candidate vaccine antigen was identified by using two-dimensional Western blot analysis .Conclusion The immunoproteomics approach could be used to identify immunogenic proteins of Aeromonas hydrophila. This study provided references for further investigation on candidate vaccine antigens.