Objective To study the curative effects for modified B-Lynch suture and uterine packing for the treatment of uterine atony postpartum hemorrhage.Methods 82 patients with uterine atony postpartum hemorrhage were randomly divided into the two groups,the observation group(n=41) and the control group(n=41).The patients in the observation group were operated through modified B-Lynch suture,while the patients in the control group were operated through uterine packing.For both groups,operative time,blood loss,blood transfusion,whether hysterectomy, puerperal morbidity,three diameter of the uterus were compared.Results Postoperative lochia without exception,no serious complications and adverse reactions occurred incision Ⅱ/A healed.The total effective rate was 100.0%. There were three cases of the control group after surgery which were ineffective hysterectomy surgery to stop bleeding, the total effective rate was 92.7%.There were significant differences between the two groups (χ2 =4.720, P <0.05).In the observation group,operative time,blood loss,blood transfusion,puerperal morbidity and hysterectomy rates were less than those of the control group (t=4.924,2.776,3.065,χ2 =5.690,4.780,P<0.05,P<0.01). The bservation group after 7d uterus three diameter were greater than that of the control group (t=2.169,P<0.05), in the 30d and 42d of the two groups,there were no significant differences in three diameter of the uterus ( t=0.624, 0.582,all P>0.05).Conclusion Treatment effect through modified B-Lynch suture for cesarean section uterine atony postpartum hemorrhage was significantly better than the uterine packing method.

Triple-negative breast cancer is defined as one of the subtypes of breast cancer recently. At present, no effective tailored therapies have been developed for patients with triple-negative breast cancer, chemotherapy is the main modality of systemic therapy for patients of this subtype. However, compared with non-TNBC, sueh treatment has a high rate of local and systemic relapse. Histologically, such cancers are poorly differentiated, and most fall into the basal- like subgroup of breast cancers. Triple- negative breast cancers have many similarities to BRCA1-associated breast cancers.

OBJECTIVE To investigate the infection of Mycoplasma and drug resistance in the patients of nongonococcal urethritis(NGU)in the local area.METHODS Totally 414 specimens in the patients of NGU were collected,cultured and tested for drug sensitvity.RESULTS From them there were 284 patients who had Mycoplasma(68.60%).The simple infection of Ureaplasma urealyticum(Uu)was 90 cases(21.74%) and that of Mycoplasma hominis(Mh) was 52 cases(12.56%).The mixed was 142 cases(34.30%).The result of drug sensitivity showed that sensitivity of Mycoplasma to minocycline(Minocin) was the highest,then was to doxycycline.Ciprofloxacin was with the highest drug resistance,then were sparfloxacin and erythromycin.CONCLUSIONS The infection rate of Mycoplasma in the patients of NGU is in a rising tendency;Mycoplasma are severely resistant to ciprofloxacin,sparfloxacin,and erythromycin,and have multi-resistance.It is important to culture and test the drug sensitivity of Mycoplasma.To control the Mycoplasma infection and supervision management of antibiotics is a urged and important task for us.

Objective To explore the effect of decrease of Survivin expression on the proliferation and apoptosis of the naso-pharyngeal carcinoma cells.Methods To observe the effect of decrease of Survivin expression on nasopharyngeal carcinoma cell morphology using RNAi technology combined with RT-PCR and Western blot.Cell proliferation and apoptosis were de-tected by MTT and TUNEL assay.The expression of PARP,Bcl-2 and Bax was detected by Western blot.Results RT-PCR result showed the survivin mRNA expression in Surviving-siRNA groups was downregulated (about 0.26±0.02)and the inhibition ratio was 43.7% (P<0.05).MTT result showed cell proliferation rates were significantly different between 24,48 and 72 h after transfection.The cell inhibition rates were 21.9%,37.1% and 29.6%,respectively (P<0.05).West-ern blot result showed the Survivin protein expression in Survivin-siRNA2 groups was downregalated and the relative ex-pression level was reduced by 57% (P<0.05).TUNEL assay showed that cell apoptosis rate was increased obviously in surviving-siRNA groups.The expression of PARP (89KD)and Bax wasupregulated (3.9 and 2.4 fold change)and the ex-pression of Bcl-2 was downregulated (0.3 fold change).The phosphorylation of AKT was inhibited when Survivin was down-regulated (about reduced by 5 7%).Conclusion Silencing Survivin gene by siRNA can inhibit the proliferation of Na-sopharyngeal carcinoma cells and induce apoptosis.Survivin may become a potential gene for therapy target of nasopharynge-al carcinoma.

OBJECTIVE To consummate the measures of prevention and control of HIV in order to effectively guard medical personnel against being infected with HIV during occupational exposure.METHODS Make a survey of the constitution of 35 medical personnel in our hospital who had directly contacted and exposed to HIV sufferers,and the main approaches of their exposure and treatment taken after exposure.RESULTS The rate of occupational exposure has reached as high as 77.78%,mainly through the skin exposure due to skinhurt.Medical personnel usually didn′t attach much attention to the potential danger of exposure,and had low capability of occupational protection.CONCLUSIONS The education of occupational safety must be strengthened,and the measures of occupation be consummate protection in order to decrease the occupational exposure of medical personnel.

Objective To investigate the expressing of Ki-67 in breast cancer tissues and explore the relationship between it and the clinic pathology characteristics.Methods Immunohistochemical SP was used to investigate Ki-67,ER,PR,C-erbB-2 expression in 120 cases of female breast cancer.Analysis the relatiouship between Ki67 and ER,PR,C-erbB-2,tunor size,lymph node metastasis,patients age,menstruation state and clinic pathology characteristics.Results Six cases' Ki-67 expression in 120 eases of female patients with breast cancer tissue were (-) (5％),36 cases' ki-67 expression were (+) (30％),31 cases Ki-67 Expression were (+ +)(25.83％),37 cases' Ki-67 expression were (+ + +) 39.17％.The expression of ki-67 did not correlate with the expression of C-crbB-2,tumor size,lymph node metastasis,patients age and menstruation state(P ＞0.05).The expression of ki-67 correlate with hormone receptor status and histology pathology classification.Conclusion Ki-67 cannot serve as an important index of evaluating prognosis of breast cancer and to guide clinical treatment.

Objective To observe the expression of ZNF139 and MMP-7 in endometrial adenocarcinoma tissues and discuss the correlation with clinical pathological significances.Methods In 71 patients diagnosed as endometrial adenocarcinoma,71 cancer tissues(adenocarcinoma group) and 71 adjacent normal tiuues(adjacent cancer group) were collected in operations.Immunohistochemical methods was used to detect the expression of ZNF139 and MMP-7 in the two groups,the relationship between the two genes and communication with clinicopathological indexes were discussed.Western blot and qRT-PCR were used to detected the expression of ZNF139 and MMP-7 at protein and mRNA level.Results The positive rates of ZNF139 was 66.20% in adenocarcinoma group,higher than in adjacent cancer group(21.42%,P=0.016);the positive rates of MMP-7 was 74.65% in adenocarcinoma group,higher than7 than in adjacent cancer group(26.76%,P=0.012).The expression of ZNF139 and MMP-7 was correlated with lymph node metastasis,muscle invasion and FIGO stage(P=0.028,0.031;P=0.004,0.016;P=0.008,0.011).Spearman rank correlation analysis showed there was positive correlation between ZNF139 and MMP-7 in adenocarcinoma tissues(r=0.716,P=0.039).Western blot showed that ZNF139 and MMP-7 expression in adenocarcinoma group were higher than in adjacent cancer group(t=6.92,7.34;P<0.01);qRT-PCR showed that ZNF139 mRNA and MMP-7 mRNA expression in adenocarcinoma group were higher than in adjacent cancer group(t=4.27,4.06;P<0.05).Conclusion ZNF139 and MMP-7 are correlated with malignant pathological characteristics in endometrial adenocarcinoma,they may modulate the malignant behavior together.

By reviewing name, type, function and indication of Chinese herbTing-Li-Zi (Lepidii seu Descurainiae) in literatures, there were northernTing-Li-Zi and southernTing-Li-Zi from Song dynasty. It was also known that northernTing-Li-Zi has strong effect and southernTing-Li-Zi had moderate effect. However, there was no strict classification between two types in current clinical practice. The disease prevention and treatment effect of Chinese herbs were depended on their internal features. Disease treatment must be based on syndrome differentiation with corresponding selection of herbs to achieve desired results. Therefore, it is necessary to combine properties with actions in the study of Chinese herbs to provide guidance in clinical practice.

Objective To evaluate the root canal deviations in vitro teeth curved root with TF and Protaper instru-ments by cone beam computer tomography( CBCT) . Methods 40 teeth in vitro in the standard collection were ran-domly assigned to two groups, prepared with the crown-down method, the TF group ready to 0. 06/#25; the Pro-taper group ready to F2 . CBCT scans were taken to measure the mesial and distal thicknesses of the tube wall in vitro teeth before and after preparation. The root canal deviation and the rate of shaft center were measured with ref-erence to the formula proposed by Gambill. Results The TF group need less time in preparation and has more effi-ciency(P<0. 05) than the Protaper group; both TF and Protaper devices appeared deformation after 5 root canal preparations in average, no instruments broken. Comparing the offset of the internal and external wall of root canal in 3, 5, 7 mm from the apex, the root canal deviation prepared by the TF group was less than the Protaper group, and its rate of axis center was greater than the Protaper group, closer to 1. Conclusion TF can maintain original root canal morphology in preparing curved root canal, also has higher efficiency;under the noninvasive condition, the root canal forming ability of preparation instruments can be evaluated by CBCT.

OBJECTIVE:To optimize the extraction technology of polyphenols from Juglans regia branch and evaluate its anti-oxidant activity in vitro. METHODS:Using extraction amount of polyphenols from J. regia branch as response value,solvent-solid ratio,extraction temperature and ethanol volume fraction as response factors,based on single factor test,response surface method was used to optimize the extraction technology of polyphenols from J. regia branch. Using vitamin C as positive control,scaveng-ing on hydroxyl radicals and 1,1-diphenyl-2-picrylhydrazyl(DPPH)radicals and total reducing activities of polyphenols from J. re-gia branch were investigated. And its antioxidant activity in vitro was evaluated. RESULTS:Optimized extraction conditions for polyphenols from J. regia branch was as follow as solid-liquid ratio of 1:25(g/mL),extraction temperature of 50 ℃,ethanol vol-ume fraction of 70%. The extraction amount of polyphenols from J. regia branch was 9.30 mg/g(RSD=0.57%,n=3)in the veri-fication test. Clearance rate on hydroxyl radicals and DPPH radicals and total reducing activity of polyphenols from J. regia branch were respectively 50.24%,95.42%,1.118 when it was under the mass concentration of 12.0,3.0,3.0μg/mL;and the related data of vitamin C was 93.71%,46.17%,0.628 under the same mass concentration(P<0.05). CONCLUSIONS:Extraction technology of polyphenols from J. regia branch optimized by response surface method is stable and feasible;polyphenols from J. regia branch shows certain antioxidant activity in vitro.