Membrane oxygen enrichment gives advantage over other oxygen source when applied to medical healthcare for being curative,safe,economical,convenient,small,light,durable,easy to operated,reliable and free from side-effect.The products related,for instance membrane oxygen enricher,membrane oxygen-bar air-conditioner,mobile oxygen-bar and so on,are the best sources of oxygen supply for long-term oxygen therapy,healthcare for healthy people and oxygen enriched room at high altitude.

Objective To develop a new method for rapid detection of E.Coli.with a new substrate Na p nitrophenyl ? D glucuronate (PNP Glucu Na). Method The test paper was immersed with of Na p nitrophenyl ? D glucuronate which was synthesized by us and another paper with culture medium and phorphate buffer.Colour change was observed to detect the E.coli. Results An E.coli concerntration of 4?10 7 took 0.5 hour, while a concentration of 4?10 0 took 12 hours. 138 strains of E.coli and 190 strains of other bacteria were examined . The sensitivity was 94.2% and the specificity was 92.6%. Conclusion This method is rapid, accurate, simple, and economical for detecting E.coli.

This study explored the current problems regarding post-doctoral scientific research station including rigid system,low fund coverage,uncooperative team,lack of innovation and independent research capacities etc.To address above-mentioned problems,the assessment process of admission to the research station should be defined,multidisciplinary academic exchanges enhanced,evaluation and incentive mechanism developed,reasonable restraint mechanism for supervisors established,and self-management of the students carried out and practical funding projects supplemented,thus cultivating a team of high-level medical talents to lead the disciplinary development.

The rat anti-HRP McAbs were prepared with rat-rat hybridoma and the rat McPAP complex was made by conjugating this McAb with HRP. The results of the present work indicated that the rat MePAP can be used for detection of antibodyantigen reaction by immunohistochemical techniques and ELISA. In both assay it gave strong staining reaction for positive antigen and with background staining appeared very pale. The sensitivity of rat McPAP and HRP labelling rabbit anti-rat IgG has been compared.

Objective To observe the expression of aquaporin-1(AQP1)in rats with acute lung injury induced by radiation,and discuss the protection of Tanreqing for rats with acute lung injury.Methods A total of 20 SD healthy rats were randomly assigned into 4 groups,including the comparison group,the radiation group(0.9％ NaCl 0.2ml),the dexamethasne group(5mg/kg)and the Tanreqing group(0.67ml/kg).Strat intraperitoneal injection one day after radiotherapy,the rats were sacrificed after 7 days.The pathological changes of lung tissues were observed by HE stain.AQP1 expressions in lung tissues were detected by immunohistochemistry and RT-PCR test.Results Compared with the radiation group,there was an obvious amelioration in pathological injury of lung tissue in the dexamethasne and Tanreqing groups.AQP1 was increased significantly in comparison group(0.8006±0.05846),but AQP1 was decreased in the radiation group (0.4938±0.03477)(P＜0.05),and AQP1 was increased after the Tanreqing and dexamethasne treatment(0.6492±0.03477,0.6664±0.03251) (P＜0.05).Conclusion Tanreqing may have beneficial effects on radiationinduced lung injury by regulating AQP1 expression in the lungs of rats.

OBJECTIVE:To establish the method for simultaneous determination of 5 saponins in Lonicerae Flos. METHODS:Using macranthoidin B as a reference,HPLC method was adopted to calculate the relative correction factor(RCF)of it to macran-thoidin A,dipsacoside B,macranthoside A and macranthoside B. The contents of above 4 saponins were calculated through RCF. Using the contents of saponins determined by external standard method as measured value,the calculated value was compared with measured value. RESULTS:The linear ranges of macranthoidin A,macranthoidin B,dipsacoside B,macranthoside A and macran-thoside B were 0.316-6.32 μg(r=0.9973),0.453-9.06 μg(r=0.9982),0.231-4.62 μg(r=0.9996),0.342-6.84 μg(r=0.9984) and 0.147-2.94 μg(r=0.9961),respectively. RSDs of precision,stability and reproducibility tests were all lower than 2.0%. The recoveries were 97.74%-104.51%(RSD=2.37%,n=6)、96.70%-103.20%(RSD=2.37%,n=6)、96.12%-103.61%(RSD=2.45%, n=6)、98.80%-104.70%(RSD=2.32%,n=6)、99.21%-102.92%(RSD=1.39%,n=6),respectively. There was no statistical sig-nificance between calculated value and measured value(P>0.05). CONCLUSIONS:The method is simple,precise,stable and re-producible. It can be used for the determination of saponins in Lonicerae Flos.

A series of reforms on the teaching of medical chemistry experiment has been carried out to arouse students' interest in medical chemistry experiment study and improve the quality of instruction,which includes the content of course,the teaching method and the inspection method.Good results have been achieved in the aspect of development of students' comprehensive quality,which lays a good foundation for the study of other courses.

Objective To explore the clinical features and endoscopic manifestations of eosinophilic gastroente-ritis(EG)in children.Methods A retrospective analysis was conducted of the clinical manifestation,laboratory examination,endoscopy(upper and/or colonoscopy)performance,diagnosis and treatment of 49 cases of patients who were diagnosed as EG in Children's Hospital of Nanjing Medical University from July 2013 to July 2015.Results The common clinical manifestations of EG in children were hematochezia(23 cases),diarrhea(20 cases),vomiting(18 cases)and abdominal pain(15 cases).The ages of children admitted to hospital for the first time ranged from 1 month and 7 days old to 13 years and 7 months old,and the mean age was 59.4 months old,in which most patients were younger than 1 year old,accounting for 38.77％(19 cases)of all.Peripheral blood eosinophilia was present in 20 cases(40.82％)of the patients,and only 6/18 cases(33.33％)of the patients had elevated serum IgE.Upon endoscopic analysis,the lesions involved esophagus(4 cases),stomach(14 cases),duodenum(20 cases),small intestine(15 cases),colon(28 cases)and rectum(6 cases),and the most common manifestation under gastroscopy was mucosal hyperemia edema(27 cases)and erosion(9 cases),while the most common manifestation under colonoscopy was mucosal hyperemia edema(25 cases)and nodular hyperplasia(24 cases).All patients improved with food restriction,in which 8 cases were treated with glucocorticoid while 9 cases with oral Singulair and 9 cases with oral Loratadine.All children with symptoms were alleviated somewhat,but 5 cases of them relapsed after drug withdrawal.Conclusions The clinical manifestations of EG in children varied and were mainly hematochezia,vomiting,diarrhea and abdominal pain.Some patients had the elevated peripheral blood eosinophilia and serum IgE.The most common manifestations under gastroscopy were mucosal hyperemia edema and erosion while the most common manifestations under colonoscopy were mucosal hyperemia edema and nodular hyperplasia.

Objective To observe the effect of continued nursing on the long-term nasal feeding to elderly patients after discharge.Methods Toally 60 elderly discharged patients between January 2014 to December 2014 were evenly divided into observation and control groups with radom digit table the control group received routine nursing before discharge and after it and the observation group received continued nursing.The two groups were compared in terms of nasal feeding-induced complication,nursing satisfaction,care capability of patients' caregivers.Result The complication rate in the observation group was significantly lower,the nursing satisfaction was significantly higher and the care capability of caregivers were significantly better as compared to those of the control group (P ＜0.05).Conclusion Continued nursing can improve nursing satisfaction,train the care capacity of caregivers,reduce the complications for the old patients who need long-term nasal feeding after discharge.

In order to improve teaching quality of medical organic chemistry,problem-based learning was introduced.The characteristic,implementary process and outlines of problem-based learning were discussed in combination with the characteristic of organic chemistry in medical university.