Objective To investigate the relationship between the life quality and the disease uncertainty of the patients with high risk of diabetic foot. Methods The data of 235 patients with high risk of diabetic foot were selected by the convenience sampling method, using the life quality scale for patients with type 2 diabetes mellitus(DMQLS revised edition) and the uncertainty of disease scale (MUIS). Results The total score on life quality in the diabetic patients with high risk was (138.04 ± 7.32), which was at a high level. The psychological dimension ranked on the top in terms of the score, following by physiological dimension, social dimension, disease dimension and satisfaction dimension. The overall score on the uncertainty was (100.09 ±6.89), which was at intermediate level. The dimension of unpredictability ranked on the top, followed by uncertainty, complexity and lack of information. Pearson correlation analysis showed that the uncertainty together with its four dimension was negatively related with quality of life (all P <0.05). Conclusions The life quality in the patients with high risk of diabetes is generally poor and their sense of uncertainty is at the moderate level. The quality of life is negatively correlated with the uncertainty of disease. So reducing the disease uncertainty is helpful for the improvement of the quality of life.

Objective To study the effect of cold and heat treatment on metastasis and the peripheral blood immune response in patients with breast cancer. Methods A total of 124 breast cancer patients were randomly di-vided into a hot and cold therapy group and a control group, each of 62. Both groups were given conventional surgical resections, and the hot and cold therapy group was given cooling and heating physical therapy for one month before the operation. The efficacy of the treatment and the patients′ spiritual state were compared after the treatment. The effect on lung metastases was evaluated using tomographic images of the chest, and the changes in immune cell levels in the peripheral blood were detected using flow cytometry. Results After the treatment, the remission rate (54.8%), spiritual life ratings on the Karnofsky scale and the area of lung cancer nodule transfer were significantly better in the hot and cold therapy group than in the control group. Twelve weeks after the treatment, the percentage of MDSCs in the control group patients was 72.5%, significantly higher than the 5.3% of the therapy group. The CD4+and CD8+ T cells were 2.2% and 1.8%, significantly lower than the 26.7% and 13.9% of the control group. Con-clusion Cold and hot therapy before a mastectomy can effectively inhibit the metastasis of breast cancer and promote patients′physical rehabilitation and spiritual life. It may regulate the microenvironment of their immunosuppression and stimulate antitumor immunity.

Objective To observe the efficacy and safety of voriconazole in the treatment for the patients infectedwith Candida species.Methods 79 patients were randomly divided into voriconazole treatment group and fluconasole treatment group.And they were enrolled and were administered voriconazole.Results The total effective rate of experimental group was 93.1％,and that of control group was 65.9％ and the difference was significant(P ＜0.05).the risk factors of the two groups showed no obvious difference.the adverse reaction incidence rate in voriconazole treatment group was 16.7％,And that in fluconazole treatment group was 14.0％,there was no significant difference between the two groups.Conclusion The application of voriconazole can receive better effect than that of fluconasole.And it has good clinical safety,and voriconazole is worthy of application in clinic for Candida infection.

Objective To investigate the effects of ephedrine on glial fibrillary acidic protein (GFAP) expression after cerebral ischemia-reperfusion injury in rats.Methods Altogether 60 male SD rats were randomly divided into sham-operation group,natural recovery group and ephedrine treatment group.The unilateral ischemia-reperfusion models were induced by clue-blocked method.The expression level of GFAP around ischemic area was examined by immunohistochemical technique at weeks 1,2,3 and 4 after operation.Results GFAP expression began to increase at 1 w and stabilized at 3 w in ephedrine treatment group and natural recovery group.There was a significant increase of GFAP expression in ephedrine treatment group compared with that in natural recovery group (P

Objective To study the different extraction methods for the content of tanshinoneⅡA in water decocted Radix Salviae Miltiorrhiza, in order to develop comprehensive utilization of Salvia miltiorrhiza. Method Tanshinone ⅡA was extracted by supercritical carbon dioxide fluid and compared with ultrasonic extraction. Result When the extraction pressure was 35 MPa, extraction temperature was 40 ℃, extraction time was 2 h and entrainer (ethanol) was 100 %, the yields of the tanshinone ⅡA was up to 3.87 mg, while the ultrasonic extraction was 2.89 mg. Conclusion TanshinoneⅡA extracted by supercritical carbon dioxide fluid was better than ultrasonic extraction and had higher purity. The comprehensive utilization of Salvia miltiorrhiza is available.

Objective To investigate the benefit of inferior vena cava filter on the treatment of deep vein thrombosis(DVT) and pulmonary embolism.Methods From January 2009 to September 2011,of the 115 patients with DVT,27 cases were treated by VCF under DSA,followed by thrombolysis and anticoagulation.Results All the cases were.successfully implanted with VCF and limb detumescence was achieved after thrombolysis and anticoagulation treatment without complications.Conclusion The implantation of inferior vena cava filter combined with thrombolysis and anticoagulation is favorable and safe to treat deep vein thrombosis and pulmonary embolism,and it is also effective to prevent PE in patients with deep venous thrombosis in the low extremity.

In comparison with the measurement of heart rate variability from ECG and from photoplethysmographic signal from 46 healthy adults in their spontaneous breathing state. The beat-to-beat intervals in ECG and pulse-to-pulse intervals in photoplethysmographic signal are extracted, and then the parameters of heart rate variability are calculated. Three kinds of algorithms are chosen to get the pulse-to-pulse intervals, which are the intervals of maximum of second derivative, the maximum of PPG signal and the tangent intersection. The results show that the correlation coefficients of the HRV parameters in the two calculation methods are highly correlated. The Bland-Altman scattered plots show the relative bias results from the algorithm of the maximum of PPG signal are smallest and singular points that deviate from the consistent limits are the least compared with the other two algorithms.
Adult ; Algorithms ; Electrocardiography ; Heart Rate ; Humans ; Photoplethysmography ; Reproducibility of Results

To investigate the effect of stepwise paced breathing (PB) on pulse transit time (PTT), we collected physiological signals of electrocardiogram (ECG), respiration and arterial pulse wave during a procedure of stepwise PB, which consists of 6 different breathing rates changing in a protocol of 14.0-12.5-11.0-9.5-8.0-7.0 breath per minute (BPM), with each breathing rate lasting 3 minutes. Twenty two healthy adults involved in this experiment and the change of PTT was analyzed during the stepwise PB procedure. In our study, the PTT was measured by calculating the time interval from the R-spike of the ECG to the peaks of the second derivative of the arterial pulse wave. Ensemble empirical mode decomposition (EEMD) was applied to PTT to decompose the signal into different intrinsic mode function, and respiratory oscillation and trend component (baseline) in PTT were further extracted. It was found that the respiratory oscillations in the PTT increased with decreasing of the PB rate, and many of the subjects (14 out of 22) showed the phenomena of PTT baseline increasing during the stepwise PB procedure. The results indicated that the stepwise PB procedure induced a high level of cardiovascular oscillation and produced an accumulative effect of PTT baseline increase. As PTT is capable of predicting changes in BP over a short period of time, increase of PTT baseline indicates the decrease of blood pressure. The experiments showed that the stepwise PB procedure could reduce blood pressure for most subjects. For future work, it is necessary to develop certain indices differentiating the effectiveness of the stepwise PB procedure on the PTT baseline change, and to test the effectiveness of this stepwise PB procedure on blood pressure reduction for patients with essential hypertension.
Adult ; Blood Pressure ; Blood Pressure Determination ; Electrocardiography ; Essential Hypertension ; Humans ; Hypertension ; Pulse Wave Analysis ; Respiration ; Respiratory Rate

This paper introduces a free and publicly open ICU database: multi-parameter intelligent monitoring in intensive care II: MIMIC-II, which has been built up and maintained by the laboratory of computational physiology at the Massachusetts Institute Technology, Beth Israel Deaconess Medical Center and Philips Healthcare over the past decade. This paper briefly introduces its infrastructure, implementation and applications in clinical studies. Clinical study pertaining to circadian variation in heart rate and blood pressure during sepsis is shown as a typical example of research performed with MIMIC-II. In this study, it was found there was significant difference in circadian variation in both heart rate and blood pressure between survival and non-survival groups in septic patients. This study tackled several important techniques necessary for the investigation of the circadian rhythm.
Blood Pressure ; Circadian Rhythm ; Critical Care ; Data Mining ; Databases, Factual ; Heart Rate ; Humans ; Medical Informatics