Objective To explore microbic distribution feature of acute rhinitis pafienta' nasal cavity and dependabihty research of respiratory infection.Methods 436 acute rhinitis pafienta were randomly divided tO infection group(314)and non-infection group(122)depended on whether accompanying respiratory infection.Take blenna narium and carry out nasal cavity microbiological detection,meanwhile take blood and mesasure the content of serum IL-4 and IFN-γ.Results The staphylococcus aureus taked the most proportion(58.6%and 3 1.2%)of nasal cavity bacterial distribution in infection group and non-infection group,the following bacterium were the staphylococcus epidermidis(44.9%and 46.7%),bacillus meningitidis purulentae(7.9%and 4.9%)and bacillus coli(3.5%and 1.6%),the major eumycete were the peptostreptococcus asaeeharolyticus(1.9%and 1.6%),eubacterium lentum (1.6%and 0)and eubacterium mucus(0.9%and 0.8%),the major virus were the syncytial virus,the rhinoviru8es,adenodrus,influenza virus,parainfluenze virus and coronaviruses.The respiratory tract infection patients' content of serum IL-4 Were significant higher(P＜0.05)than the non-infection group,but the content ofIFN-γ were signifieanfly reduced(P＜0.05).Conclusion The staphylococcus aureus,syncytial virus,rhinoviruses,adenovirus and influenza virus have the close relation with the infection of the respiratory tract,which can cause the disorder of organism immune function.

BACKGROUND:Microleakage between restoration, tooth structure and bonding agent can cause the entry of bacteria and liquid in the mouth into the gap, thereby damaging the bonding interface between the restoration and tooth tissues, and leading to bond failure. Microleakage detection can directly show whether the closure of the root canal of post and core system is good or bad. The severity of microleakage directly affects the restorative effects of post and core. OBJECTIVE: To evaluate the effects of different root canal cleaning methods on the microleakage between the fiber post and root canal dentin. METHODS: Thirty fresh non-caries premolar posts with free root canalin vitro were randomly divided into five groups, and the root canal wal was respectively washed with saline, 5.25% sodium hypochlorite solution+17% ethylenediamine tetra-acetic acid (EDTA)+saline, 3% hydrogen peroxide solution+5.25% sodium hypochlorite+ saline, 3% hydrogen peroxide solution+2% chlorhexidine solution+saline, and 2% chlorhexidine solution+17% EDTA+saline in different groups. Super-bond C&B adhesive agent was used for bonding fiber post, and the microleakage of each sample was observed under stereomicroscope. RESULTS AND CONCLUSION: The severity of microleakage in the al groups was ranged as folows: saline group > 3% hydrogen peroxide solution+5.25% sodium hypochlorite+saline group > 5.25% sodium hypochlorite solution+17% EDTA+saline and 3% hydrogen peroxide solution+2% chlorhexidine solution+saline groups > 2% chlorhexidine solution+17% EDTA+saline group.

Objective: To analyse the effects of physical activity on neurocognitive function in children with autism spectrum disorder (ASD),so as to provide a theoretical basis and specific guidance for the clinical cognitive function rehabilitation practice of ASD children. Methods: Relevant literature on the effects of physical activity on neurocognitive function in children with ASD were searched in the China National Knowledge Infrastructure (CNKI), Wanfang, Web of Science, and EBSCO databases from their inception to March 2024 using specific subject terms. Information on authors, countries, publication dates, study populations, diagnostic criterion, interventions, measurement tools and study results were extracted postscreening. The methodological quality of the articles was assessed using the PEDro Scale, followed by a systematic review. Results: A total of seventeen articles and were included in the study and English literature, most of them were of good quality. The study included 10 randomized controlled trials, 4 quasiexperimental designs, and 3 preand posttest designs. The rehabilitative effects of physical activity on children with ASD were primarily observed in enhanced attention and executive function, as well as improvements in brain structure and function. Children with ASD showed varying cognitive effects in physical and skillbased programs, with skillbased activities like basketball and table tennis notably improving cognitive function. Conclusion Physical activity is an effective intervention that enhances cognitive abilities such as attention and executive function, as well as brain structure and function in children with ASD.

Objective To review the application of artificial intelligence(AI)in the identification,monitoring and intervention of sleep disorders in the elderly and the effect. Methods A scoping review was conducted by searching relevant literature ahout the application of AI in the field of sleep disorders among the elderly from databases including PubMed,Web of Science,CNKI,and Wanfang data,covering literatures from the establishment to June,2024. Results A total of ten articles were included,originating from seven countries and involving 36 344 elderly participants.The publication dates ranged from 2020 to 2024.The study types included six cross-sectional studies,one pro-spective study,one self-controlled study and two randomized controlled trials.The articles mainly came from the fields of clinical medicine,sleep research,rehabilitation medicine and information engineering.AI was primarily used for monitoring the entire sleep process of the elderly,predicting and identifying sleep disorders,and inter-ventions such as biofeedback,online consultations and cognitive-behavioral therapy based on mobile platforms. Conclusion AI not only improves the accuracy of diagnosing sleep disorders,but also provides robust data support for clinical intervention.Online sleep interventions based on big data and intelligent algorithms can offer effective health management for the elderly.

Objective To review the application of artificial intelligence in anxiety and depression among children and adolescents. Methods PubMed,Web of Science,CNKI and Wanfang data were searched for literature related to artificial intelligence applied in of anxiety and depression among children and adolescents from January,2014 to January,2024,and a scoping review was conducted. Results A total of ten papers were included,originating from the United States,China,Australia and France.These re-searches were primarily published in journals related to psychology,public health,clinical medicine,neurosci-ence and rehabilitation,involving six cross-sectional researches,three longitudinal researches and one interview,including 10 880 participants aged from three to 18 years.The types of data mainly included questionnaires/scales,interview data and brain imaging data.Features related to anxiety and depression were extracted from the data,and artificial intelligence were employed to develop models for the identification or prediction of anxiety and depression in children and adolescents. Conclusion Artificial intelligence is potential for identifying or predicting anxiety and depression in children and adoles-cents.

As China enters the aging era, the incidence rate and prevalence of inflammatory bowel disease(IBD)in the elderly have increased.The relationship between aging and inflammation appears to be more significant in elderly individuals with IBD.However, most current studies focus on describing the clinical characteristics of the disease, with limited research on its pathogenesis and treatment.In this review, we summarize the differences in clinical characteristics and treatment between elderly individuals with IBD and young individuals.We also discuss the impact of aging on elderly IBD and explore the possibility of stem cell transplantation therapy as a new approach for clinical management.Our aim is to provide fresh insights for the treatment of elderly IBD.

Objective: To investigate the development of hepatocyte senescence during liver fibrogenesis and to explore the effect and possible mechanism of insulin-like growth factor 1 (IGF-1) on hepatocyte senescence and liver fibrosis. Methods: A total of 42 male Sprague Dawley (SD) rats were selected. Eighteen rats were induced by carbon tetrachloride (CCl₄) to establish the rat model of liver fibrosis. On the day 0, six and 28 after the establishment of the model, six rats were executed respectively to analyze the liver fibrosis and hepatocyte senescence in CCl₄-induced liver fibrosis rat models. Twenty-four rats were divided into control group, CCl₄ group, CCl₄+ lentivirus vector (LV-CTR) group and CCl₄+ LV-IGF-1 group, with six rats in each group.The rats were sacrificed on the 28th day after the establishment of the model. The liver tissues were obtained and the inferior vena cava blood was collected to analyze the effect of IGF-1 overexpression on liver fibrosis and hepatocyte senescence. Analysis variance (ANOVA), least significant difference (LSD) and Dunnett T3 test were performed for statistical analysis. Results: Steatohepatitis on the 6th day and early stage of hepatic fibrosis on the 28th day, which indicated the model was successfully established. The results of the effects of IGF-1 overexpression on hepatic fibrosis and hepatocyte senescence showed that on the 28th day, compared with those of control group, both the score of Ishak liver inflammation and necrosis and the score of Ishak liver fibrosis were increased in the CCl₄ group, CCl₄+ LV-CTR group and CCl₄+ LV-IGF-1 group (0, 14.55±1.94, 15.43±2.19 and 10.29±1.47, respectively; 0, 3.51±0.51, 3.21±0.79 and 1.32±0.40, respectively). The area of liver tissues by Masson staining (0.45±0.40, 5.62±1.08, 6.03±0.65 and 2.88±1.54), SA-β-Gal staining (1.75±0.80, 4.28±1.19, 4.92±1.14, 3.11±0.79), p53 (2.02±0.81, 4.36±1.02, 4.72±0.72 and 3.58±0.70) and progerin (0.72±0.40, 4.52±1.01, 4.01±1.25 and 2.66±0.80) all were increased. The levels of serum IGF-1 all were decreased ((632.00±6.04), (503.00±40.42), (508.00±21.94) and (572.40±5.94) ng/L). However the levels of ALT all were increased ((11.20±5.97), (214.00±73.90), (245.00±76.06) and (30.00±5.00) U/L). The relative expression levels of p53 (0.58±0.06, 1.78±0.18, 1.72±0.10 and 1.23±0.22) and progerin (0.12±0.02, 0.78±0.15, 1.32±0.20 and 0.81±0.16) in the primary hepatocytes were increased. The differences were all statistically significant (F=91.674, 90.778, 32.982, 9.726, 10.640, 17.029, 103.910, 30.059, 64.707 and 97.457, all P<0.05). Compared with those of CCl₄+ LV-CTR group, the score of Ishak liver inflammation and necrosis and the score of Ishak liver fibrosis were decreased in the rats′ liver tissues of CCl₄+ LV-IGF-1 group, the areas of Masson staining, SA-β-Gal staining, p53 and progerin in the liver tissues were decreased, the level of serum IGF-1 was increased, the level of ALT was decreased, and the relative expression levels of p53 and progerin in primary hepatocytes both were decreased. The differences were all statistically significant (all P<0.05, respectively). Conclusions Hepatocyte senescence increases in the process of liver fibrosis induced by CCl₄. Overexpression of IGF-1 may alleviate liver injury, improve hepatocyte senescence and liver fibrogenesis by regulating the nuclear p53/progerin pathway.

Objective To explore the affect of physical activity on neurocognitive function of overweight children.Methods The literatures about the effect of physical activity on neurocognitive function of overweight children were retrieved with subjected retrieval method in CNKI, VIP, Wanfang database, PubMed, Web of Science, EBSCO databases from January, 2000 to January, 2022. The authors, countries, published years, ages of subjects, research methods, intervention time, measurement tools and measurement indicators were extracted.Results Twelve articles were selected finally. These articles came from United States, Italy, Belgium and China, were mainly published in journals of psychology, clinical medicine, neuroscience, physical education. The study design included randomized controlled trials and pre-post-trial. The mean PEDro score was 6.67. The types of physical activity included fitness, skills and sports activities. The overweight children participating in physical activities could benefit from cognition and brain plasticity. On the cognition, physical activity could improve attention, memory, inhibitory control, working memory and cognitive flexibility. In terms of brain plasticity, physical activity could improve the integrity of brain white matter, increase hippocampal volume, enhance brain activation and brain network connectivity.Conclusion Physical activity is a simple and effective form of exercise. It can improve not only attention, memory, executive function and other cognitive functions, but also brain structure and brain function in overweight children. The mechanism of cognition, the interaction between physical activity and other factors, and the dose-relationship between physical activity and cognitive benefits still need to be explored in the future.

Objective: To establish a set of rules for autoverification of blood analysis, in order to provide a way to validate autoverification rules for different analytical systems, which can ensure the accuracy of test results as well as shorten turnaround time (TAT) of test reports. Methods: A total of 34 629 EDTA-K2 anticoagulated blood samples were collected from multicenter cooperative units including the First Hospital of Jinlin University during January 2017 to November 2017. These samples included: 3 478 cases in Autoverification Establishment Group, including 288 cases for Delta check rules; 5 362 cases in Autoverification Validation Group, including 2 494 cases for Delta check; 25 789 cases in Clinical Application Trial Group. All these samples were analyzed for blood routine tests using Sysmex XN series automatic blood analyzers.Blood smears, staining and microscopic examination were done for each sample; then the clinical information, instrument parameters, test results and microscopic results were summarized; screening and determination of autoverification conditions including parameters and cutoff values were done using statistical analysis. The autoverification rules were input into Sysmex Laboman software and undergone stage Ⅰ validation using simulated data, and stage Ⅱ validation for post-analytical samples successively. True negative, false negative, true positive, false positive, autoverification pass rate and passing accuracy were calculated. Autoverification rules were applied to autoverification blood routine results and missed detection rates were validated, and also data of autoverification pass rate and TAT were obtained. Results: (1)The selected autoverification conditions and cutoff values included 43 rules involving WBC, RBC, PLT, Delta check and abnormal characteristics. (2)Validation of 3 190 cases in Autoverification Establishment Group showed the false negative rate was 1.94%(62/3 190)(P<0.001), autoverification pass rate was 76.74%, passing accuracy was 97.47%; Validation of 2 868 cases in Autoverification Validation Group, the false negative rate was 3.38%(97/2 868)(P=0.002), autoverification pass rate was 42.26%, passing accuracy was 92.00%; Validation of Delta check on 288 cases in Autoverification Establishment Group and 2 494 cases in Autoverification Validation Group showed the false negative rates were respectively 1.39% and 2.61%(P<0.001). (3)Three hospitals adopted these rules of autoverification for 25 789 blood routine samples, and found that the average TAT of blood routine test reports were shortened by 24min, 32min and 7min respectively, the rate of samples reported within 30min were elevated by 33%, 53% and 7%. The autoverification pass rates were 72%-74%. Conclusions The application of this set of 43 autoverification rules in blood sample analysis can ensure test quality while shortenTAT and improve work efficiency. It is worth pointing out that for the same analytical systems in this research, validation is necessary before application of this set of rules, and periodic validation is required during application to make necessary adjustment; for different analytical systems, as this research provide a way to establish autoverification rules for blood routine tests.Clinical labs may establish their own suitable autoverification rules on the basis of technological parameters. (Chin J Lab Med, 2018, 41: 601-607)

To construct cellular senescence model by stimulating primary hepatocytes with hydrogen peroxide (H 2O 2). Primary hepatocytes were transfected with p53 siRNA, progerin siRNA or IGF-1 adenovirus vector. The number of SA-β-Gal stained positive cells and the expression of p53 and progerin were detected. The results showed that p53 siRNA and progerin siRNA had knocked-down the expression of p53 and progerin, and had alleviated the hepatocyte senescence. Transfection of insulin-like growth factor (IGF)-1 adenovirus vector into primary hepatocytes had overexpressed IGF-1, and had alleviated the number of SA-β-Gal-positive cells. The expression of p53 and progerin was down-regulated in the nucleus, while the expression of p53 was up-regulated in the cytoplasm. The co-precipitation and co-localization of p53 and progerin was decreased in the nuclear region of hepatocytes. IGF-1 overexpression can inhibit intranuclear p53 translocation, alleviate the interaction between p53-progerin, and alleviate hepatocyte senescence.