BACKGROUND: Thoracic outlet syndrome can be divided into nervous system type and vascular type according to different compressed structure. Nervous system type has typical clinic manifestations,physical signs,radiological evidence and electromyogram variation. So,there is wide prospect for clinic application about evaluation of compression of nerve in patients with thoracic outlet syndrome by electrophysiological examination.OBJECTIVE: To study diagnostic significance of electrophysiological examination to thoracic outlet syndrome.DESIGN: A completed and randomized research based on patients.SETTING: An electrophysiological department in a municipal hospital.PARTICIPANTS: Twenty patients diagnosed as thoracic outlet syndrome were collected in Neurology and Neurosurgery Department,Hand Surgery Department and Clinic of Dalian Central Hospital from April 1998 to July2002,in which there were 5 males and 15 females,aged 31 -67,average46.0 years,with course of 2 - 7 years,average 4.5 years.METHODS: Nerve conduction velocity,wave amplitude and corresponding muscular needle electrode electromyogram(EMG) of median nerve,ulnar nerve,interior and exterior cutaneous nerve in forearm,suprascapular nerve,axillary nerve,musculocutaneous nerve,radial nerve were tested by keypoint myoelectricity evoked potential equipment made in Denmark.MAIN OUTCOME MEASURES: The results of nerve electrophysiological examination.RESULTS: The type of compression of nerve trunk manifested as slower sensory and motor conduction velocity of nerve dominated by corresponding trunk across axillary section,lower wave amplitude and loss of nerve of corresponding muscle.CONCLUSION: Nerve electrophysiological examination is of important significance to diagnose thoracic outlet syndrome.

Bladder cancer is a common tumor with a high rate of recurrence and quick progression.Many biomarkers for the detection of bladder cancer have been identified,but none has been reported to date to have sufficient sensitivity and specificity.Recent researches are concentrated on p53,Rb,Cyclin D,P27kip-1,FGFR3,Cadherin,Survivin,Aurora,MMP,VEGF and DNA Methylation.In spite of their different sensitivities for bladder cancer of different stages or degrees,these biomarkers are valuable for its diagnosis and prognosis.

Objective: To investigate the role of clinical pharmacist in anticoagulant therapy.Methods: Clinical pharmacist participated in the anticoagulant treatment adjustment for one patient with deep vein thrombosis (DVT) of lower limbs.Clinical pharmacist assessed the risk factors of DVT and the bleeding risk of anticoagulant therapy, provided suggestions in case of over-anticoagulation with warfarin, and offered medication education to the patient with unsatisfactory compliance.Results: Physician adopted the suggestions of clinical pharmacist, INR was within the rational range, and the patient was discharged with stable condition.Conclusion: The participation of clinical pharmacist in the anticoagulation with warfarin can reduce the risks of drug use and promote the efficacy and safety of medication effectively.

This paper proposes a falling detection system based on MPU6050 senor. The system consists of a MPU6050 sensor, a STM32 MCU and a set of Bluetooth 4.0 device: collecting and parsing the falling data, transferring the result to a smartphone, the smartphone: receiving the result, alarming the elder's family and hospital. This paper presentes a new judging algorithm based on the threshold of three-axis acceleration and angle deviation of body, in order to differentiate AF and normal daily activity. The result proves that the accuracy of the system is higher than 95%, which strongly highlight the robustness and reliability.
Acceleration ; Accidental Falls ; Activities of Daily Living ; Aged ; Algorithms ; Humans ; Reproducibility of Results ; Smartphone ; Software

Objective To investigate the effect of diazepam injection on the degree of cervical dilatation, delivery outcome and negative emotion in the first stage of labor. Methods Retrospective study of 120 cases of maternal birth in our hospital as a study sample. Diazepam was used in the first stage of labor, and 60 cases of women with worse cervical dilatation and negative mood were selected as observation group, the other selected not to use any drug-assisted childbirth, only to take the basis of comfort and other interventions, the clinical symptoms and observation group were similar to 60 cases of maternal were classified as control group. The effects of the two groups intervention on maternal mood, delivery outcome and cervical dilatation were compared. Results The self-rating anxiety scale (SAS) and self-rating depression scale (SDS) scores of the observation group were significantly lower than those of the control group at 1h, 12h and 24h after treatment, the difference was statistically significant (P<0.05). Observation group Bishop cervix related degree of cervical dilatation, cervical soft and hard, cervix position, cervical canal disappearance and fetal exposure were significantly higher than those of the control group, the difference was statistically significant (P<0.05). The rate of spontaneous delivery in the observation group (83.33%) was significantly higher than that the control group (56.67%), the difference was statistically significant (P<0.05). There were no statistical differences in the rate of adverse childbirth in the observation group (11.67%) and the control group (10.00%). Conclusion The use of diazepam injection in the first stage of labor can effectively improve the overall maturity of maternal cervix, relieve maternal depression, anxiety and other negative psychological, and drug safety is high, and there was no significant adverse effect on the outcome of their childbirth.

Objective:To enhance hospital equipment information management standards for the purpose of measurement instruments.Methods:Using solutions based on Web technology, database technology, database through the establishment of measurement devices, implement life cycle management method.Results: To solve the many hospital only attach importance to the management of medical equipment, ignore the metering device management, lead to measuring instruments record disorder, query the inconvenience problem.Conclusion: Medical device measurement equipment management system improves the ability of the hospital information management, strengthens the efficiency of communication and exchanges of various departments and produces good economic and social benefits.

Enantioselective hydrolysis of 2-carboxyethyl-3-cyano-5-methylhexanoic acid (CNDE) is the key step in chemoenzymatic synthesis of pregabalin. We purified an intracellular carboxyl esterase from Morganella morganii ZJB-09203, which exhibited high enantioselectivity and activity towards CNDE. The carboxyl esterase was purified to electrophoretic homogeneity by ammonium sulfate fraction precipitation, Phenyl Sepharose 6 FF hydrophobic interaction chromatography, anion exchange with DEAE Sephadex A-50 and Bio-Scale CHT column. The purified enzyme was a monomer with molecular mass of 68 kDa determined by SDS-PAGE and gel chromatography. Substrate specificity of the enzyme towards p-nitrophenyl esters suggested that the purified enzyme was an esterase. The optimal reaction pH for CNDE hydrolysis was 9.0, and optimal temperature was 45 degrees C. The esterase was stable between pH 7.0 and 9.0, and at 40 degrees C. The enzyme activity was enhanced by Ca2+, Cu2+ and Mn2+, whereas strongly inhibited by Co2+, Fe3+, Ni2+ and EDTA. Meanwhile, we investigated the kinetic parameters of the esterase towards p-nitrophenyl esters and effect of CNDE concentration on conversion. The present study reported the esterase capable of stereospecific hydrolysis of CNDE for the first time. Our research will provide foundations for industrial production of Pregabalin using the new biocatalyst.
Chromatography, Gel ; Electrophoresis, Polyacrylamide Gel ; Esterases ; metabolism ; Hydrogen-Ion Concentration ; Kinetics ; Molecular Weight ; Morganella morganii ; enzymology ; Substrate Specificity ; Temperature

Objective To investigate the expression of MMP-2 and MMP-7 and their elinieopathologieal significance in eoloreetal carcinomas. Methods The expression of MMP-2 mRNA, MMP-7 mRNA and MMP-2, MMP-7 in 42 samples of eoloreetal carcinomas and its adjacent normal eoloreetal mueosa were examined using fluo-rescence quantitative RT-PCR (FQ-RT-PCR) and immunohistoehemistry. The relationship of their expression with some elinieopathologieal characteristics was analyzed. Results MMP-2 mRNA, MMP-7 mRNA and MMP-2, MMP-7 were significantly over-expressed in eoloreetal carcinomas compared with its adjacent normal eoloreetal mueosa( P < 0.05 ) , and they were positively associated with bowel wall invasion, the regional lymph node metastasis and Dukes stage. The expression of MMP-2 mRNA and MMP-7 mRNA was positively correlated with MMP-2 and MMP-7 in eoloreetal eareinornas. Conclusions MMP-2 and MMP-7 may play a key role in eoloreetal carcinogenesis,tumor in-vasion and metastasis. Examination of combined MMP-2 and MMP-7 expression may have an important significance to judge the malignant degree and biological behavior of human eoloreetal carcinoma and for evaluating the progno-sis.

Objective To analyze the clinical characteristics of hospital acquired mycotic pneumonia in elderly patients (aged≥ 80 years).Methods The clinical data were reviewed on 64 cases of elderly patients aged 80-93 years with hospital-acquired infection of mycotic pneumonia from June 2007 to July 2011.According to the results of sputum culture,therapy plan was made and antibiotic drugs were selected.Results Among these 64 patients,Candida mycoderma (62.5 ％,40 cases) occupied the first place and C.glabrata (20.3％,13 cases) was the second place (x2 =127.50,P＜0.01).Their chest x-ray or CT films were not characteristic,but lamellar shadows (68.8％,44cases) and cotton-like shadows (40.6 ％,26 cases) were found in the majority.60 cases (93.8 ％ ) of these patients had more than 3 complications,and 58 cases (90.6％) of them took over 2 kinds of antibiotics.The improvement rate of these patients was 81.3％ (52 cases)and mortality rate was 18.8％(12 cases).Conclusions Elderly patients (aged≥ 80 years) with hospital acquired infection of mycotic pneumonia have high incidence and mortality rate.The key point to cure is to make an early diagnosis and treat them as early as possible.

Object To investigate the saccharide changes of Radix Rehmanniae when being processed. Methods The fresh roots were torrefied at 65 ℃ and sampled at 0,1, and 6 d. The samples and 4 h steamed slices of those dried roots were extracted with hot water respectively. The extracts were subjected to HPLC analysis, Sugar pak 1 column at 80 ℃,with water as the mobile phase at 0 7 mL/min and detected by RID. Results There are three principal components in the fresh roots, i.e. stachyose (11%-15%), sucrose (0 30%-0 92%), and catalpol (0 27%-0 88%). In the processing of the fresh roots, the HPLC chromatorams of the extracts differed to each other remarkbly. On the chromatogram of roots torrefied for 1 d, one distinct monosaccharide peak displayed at 10 2 min, which is likely to be galactose, and it become prominent in the dried roots together with raffinose. In steamed ones, the peaks of fructose and glucose became outstanding. Conclusion According to the HPLC chromatograms, the degalacto sylation of stachyose may take place during torrifying, while defructosylation during steaming. One of the principal purposes of the processing may be for stachyose degradation for its flatulence causing character.