Neovascularization is one of the crucial challenges in tissue-engineered grafts.In order to find sdution to the problem,endothelial cells and animals were exposed to electromagnetic fields to examine the formation of new vascular.This review gives an overview of the effects of electromagnetic fields on the neovascularization of tissue-engineered grafts.

Objective:To evaluate the effect of agar-oliogsaccharides (mainly containing agarobiose) on blood glucose level and oxidation-antioxidation status in alloxan-induced diabetic mice. Method: The inhibitory activity of agarobiose on ?-glucosidase was investigated. Alloxan was used to establish non-obese diabetic (NOD) mice model. After 14 d administration of agarobiose, blood glucose, MDA, SOD, GSH and GSH-Px levels were determined. Results:Agarobiose showed inhibitory activity on ?-glucosidase with IC50=1.39 mg/ml, Ki=0.52 mg/ml. In a certain concentration (200-400 mg/kg bw), agaro-oligosaccha- rides reduced the blood glucose level remarkably, and the oxidative indices were also improved in heart, liver, lung and brain. The blood glucose level (P

Diffuse large B-cell lymphoma(DLBCL),the largest category of lymphoma,is a clinically and biologically heterogeneous disease entity.Immunophenotype,tissue microarray and molecular studies underline the extreme heterogeneity of DLBCL and suggest a subclassification of the tumor.This review summarizes the morphological,immunophenotypic,cytogenetic and molecular genetic features of DLBCL relevant to the precise prognostic previsions.

The source, super-antioxidant activity, biological safety and application in medicine, health food and animal feed industry of natural astaxanthin are reviewed in present paper. The industrialization prospect of astaxanthin in China is also proposed.

Objective:To select the differentially expressed vascular smooth muscle cell(VSMC) apoptosis gene induced by icariin,glucose regulated protein 78(GRP78),to observe the expression of GRP78 in atherosclerosis tissue and to the elucidate the mechanism of anti-atherosclerosis of icariin.Methods:The model was established by using halfcystine in viro.The vascular smooth muscle cell was incubated with various concentrations of icariin for 48 hours.The apoptosis was detected by TUNEL assay.GRP78 gene was amplificated by PCR,recombinant plasmid were constructed,transfected into E.coli,positive clonings were selected and conf irmed by restriction endonucleas analysis,and sequence-ed.Tissue situ hybridization was used to observe the GRP78 expression in atherosclerosis tissue.Results:Icariin promoted obviously vascular smooth muscle cell apoptosis.Furthermore,the fragment,648bp,was similar to the gene of GRP78 from VSMC,and the expression level was signif icantly higher in high cholesterol group than in control nroup.Conclusions:The GRP78 plays a key role in inducing atherosclerotic lesions.The mechanism of anti-atherosclerosis of icariin might be partly due to promoting apoptosis of vascular smooth muscle cell.

The glutathione peroxidase cDNA has been acquired by reverse transcriplate-polymerase chain reaction (RT-PCR). mRNA was isolated from the culture liver cancer cells firstly, then trancriplate if into cDNA with AMV reverse transcriptase. By using this cDNA as PCR template and two synthetic special primers, we get about 600 bp DNA fragment after 35 circles. The result is identical with our expectation.

Objective To know the prevalence rate of hypertension and the rates of illness-awareness,medication and blood pressure control in Wenzhou. Method With random cluster sampling,1 034 residents aged≥15 yrs were selected from the communities of Wenzhou,and investigated with the standard questionnaires. Results The prevalence rate of hypertension in Wenzhou was 38.97% and the standardized rate was 22.27%. Among the patients with hypertension,51.12% of them were aware,and only 40.69% of them took medication and 9.18% of them had their blood pressure controlled. Conclusion The prevalent rate of hypertension is higher,but the awareness rate,medication rate and controlling rate are lower in Wenzhou city. The comprehensive interventions are needed for the prevention and treatment of hypertension.

Objective:To observe the influence of erythropoietin (EPO)combined adjuvant iron treatment on cardiac function,exercise capacity and anemia in patients with diastolic heart failure (DHF)complicated anemia.Methods:A total of 94 DHF + anemia patients were randomly divided into routine treatment group (n=46)and combined treatment group (n=48,received EPO combined adjuvant iron treatment based on routine treatment).Patients re-ceived 6min walking test,levels of hemoglobin (Hb)and N-terminal pro brain natriuretic peptide (NT-proBNP), transmitral early diastolic peak velocity deceleration time (DT),early diastolic peak flow velocity (E),late diastolic peak flow velocity (A)and E/A etc.were measured and compared between two groups before and after six-month treatment.Results:Compared with before treatment,after treatment,NT-proBNP and DT significantly reduced, E/A and 6min walking distance significantly rose in both groups,P <0.05 or <0.01;compared with routine treat-ment group after treatment,there were significant reductions in NT-proBNP level [(368.4±110.7)pg/ml vs.(239 ±89.7)pg/ml]and DT [(257.5±73.4)ms vs.(208.9±59.1)ms],and significant rise in Hb level [(83.3±9.2) g/L vs.(122.1±13.6)g/L],E/A [(0.87±0.3)vs.(1.07±0.27)]and 6min walking distance [(367.54±21.08) m vs.(438.63±20.35)m]in combined treatment group,P <0.05 all.Conclusion:Erythropoietin combined iron treatment can better improve heart function and exercise capacity in patients with diastolic heart failure complicated anemia.

Objective Agaro-oligosaccharides have been proved to possess the antioxidative ability in vitro. This work focus on the preparation of agaro-oligosaccharides and their antioxidant effect in vivo. Method Agaro-oligosaccharides were hydrolytically obtained and the activated carbon column was used to purify the oligosaccharides. The antioxidative effects of the 10 % ethanol eluted fraction on tissue peroxidative damage induced by carbon tetrachloride (CCl 4) was also investigated in rat. Result Agarobiose of high purity was consequently eluted from chromatography in the fractions of 5 % and 8 % ethanol, agarotetraose and agarohexaose in 10 % to 15 % ethanol, as well as high degree of polymerization (DP) oligosaccharides in 25 % ethanol. The antioxidative results indicated that agaro-oligosaccharides could elevate the activities of SOD, GSH-Px and decrease the level of MDA, GPT, GOT significantly. At a dose of 400 mg?kg~ -1, MDA level was reduced 44 % and 21 % in liver and heart, the activities of SOD and GSH-Px would amount to the peak value in liver and serum, while GPT level was decreased 22 % in serum. Conclusion The purity of agaro-oligosaccharides with different range of DPs was improved by activated carbon column, which possesses the property of different absorbance ability towards oligosaccharides with various DPs and high isolating capability. The animal test also indicated that agaro-oligosaccharides could inhibit the oxidative damage in vivo.

The essence of thinking was the procedure for processing information by knowledge in the frame of world view,which depending on the world view and the existing knowledge and information.The characteristic of traditional Chinese medicine (TCM) thinking was reflected by its unique world view.The world view of TCM thinking was natural view,holism concept and dialectic view,compared with modern scientific thinking mostly in reduction analysis.To develop TCM,the modern TCM thinking system was established through the reforming and innovating of TCM thinking characteristic based on preservation and promotion of them.