The treatment for chronic myeloid leukemia is still a challenge,at present.Tyrosine kinase inhibitors can partially control chronic myeloid leukemia in blast crisis (CML-BC),but the effect is not durable.Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is the only way that can cure CML-BC.This paper presents a review of the treatment before allo-HSCT for CML-BC,the selection of condition regimen,donor and hematopoietic stem cell,the monitoring of MRD post-transplant,the prophylaxis and intervention of relapse.

Through thorough understanding of the pathophysiology of graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL) effect, especial the effects of immune cells and cytokines, the article is designed to find out multiple ways to reduce GVHD and reserve or enhance GVL effect. Many kinds of cytokines are involved in the pathophysiologic mechanisms of GVHD and GVL effect. Different cytokines have different effects on GVHD and GVL effect. Immune cells play important roles in regulating GVHD and GVL effect. Studies shows that some cytokines and specific immune cells are able to reduce GVHD, as well as reserve or enhance GVL effect at the same time, which make the separation of GVL from GVHD can be achieved. On the whole, through thorough understanding of the mechanism of immune cells and cytokines in GVHD and GVL effect, we can find out multiple ways to reduce GVHD, as well as reserve or enhance GVL effect.

Objective To provide an information monitoring software for therapy drug,which can automatically construct safe concentration scope,statistically analyses data,query and input data.Methods TDMIS is developed by Powerbuilder 9.0 and is run in WIN 98 or the copy over it.2062 cases are analyzed through TDMIS and a safe concentration scope is set.Conclusion TDMIS is a practical software.It can make drug prescription standardized,computerized and easy to be statistically analyzed.The working efficiency and quality of clinical apothecary are greatly improved.

According to the programming characteristics under the SOC embedded environment,the dynamic auto-adapted bit stream technology,the intelligent interception technology,and the tele-control technology are utilized to realize the real time network communication of medical video with a variable bit stream from 1M to 4M as well as the tele-control of multiple communication protocols such as TCP,UDP and Multicast.

OBJECTIVE:To study influence factors of medical students’rational drug use in clinical practice and to provide references for improvement of medical educations and rational drug use. METHODS:The information was obtained by 325 designed questionnaire"questionnaire for prescription behaviors of medical students’rational drug use"performed by students in two medical colleges of Jiangxi Province and was inputted using Epidata3.0 software. SPSS13.0 software was adopted to analyze data by means of descriptive statistical analysis and multiple linear regression analysis methods. RESULTS & CONCLUSIONS:The level of medical students’rational drug use was just a little below the average level. It was positive correlated to the self-evaluation on instructors’conducting frequency and the achievements in medical course,but with negative correlation to the performances of rational drug use knowledeg. The achievements in medical course in self-evaluation has great impact on rational drug use behaviors while the performances of rational drug use knowledge has little impact on it.

The popularization of home medical equipment facilitates the treatment and management of many diseases, improving the patient compliance. However, due to the absence of medical background, most of their users have various security risk in the course of reorganization, choosing, using and maintenance of the products. This paper analyzed the property of security risk in home medical equipment, and described the matters needing attention in the using of several common products.
Consumer Product Safety ; Equipment and Supplies ; Household Articles ; Risk Assessment

Objective To investigate the efficacy and safety of oral ganciclovir on cytomegalovirus viremia in patients receiving allogeneic stem cell transplantation.Methods Thirty patients who were treated with oral ganciclovir from Feb,2006 to May,2006 were studied.Intravenous ganciclovir was used 10 mg/(kg?d)for eight days before HSCT as prophylaxis of CMV infection.Cytomegalovirus was detected after HSCT once a week using polymerase chain reaction(PCR)method.If CMV was positive and CMV-DNA load was higher than 6.0?102 copies/mL and lower than 105 copies/mL,oral ganciclovir was used for the treatment(1 g,three times a day).Results CMV viremia was positive at a median time of 42 days after HSCT;median CMV-DNA load was 4.626?103 copies/mL.Good response on CMV DNA load(reduction below 6.0?102 copies/mL)was observed in 90% of oral ganciclovir and the response rate was 70.0% within 14 days.Median duration of therapy for good response was 10 days(range 2~30 days).Severe adverse effects were not observed and CMV-related disease did not occur.Conclusion Oral ganciclovir is an attractive and safe alternative for pre-emptive CMV viremia treatment in allo-HSCT recipients.

Objective To investigate the relationship between the expression of metastasis associated antigen 1 (MTA1) and the biological behavior of endometrial carcinoma. Methods From May 2005 to May 2007, 30 patients with endometrial carcinoma and 20 patients with normal endometrial tissues, were admitted to Changzheng Hospital of the Second Military Medical University for surgical treatment were involved in the present study. The expression of MAT1 mRNA in two different groups was detected by real-time RT-PCR, and of MAT1 protein was detected by immunohistochemical streptavidin-peroxidase (SP) method and Western blotting. The relationship between their expression and pathological features were analyzed. Results The positive expression rate of MTA1 protein was 80% in endometrial carcinoma group, which was significantly higher than that in control group (30%, P0.05). Conclusion The expression of MTA1 might play a potential role in the pathogenesis and development of endometrial carcinoma, and it might be a novel target for the therapy and prognostic indicator of endometrial carcinoma.

[ ABSTRACT] AIM:To explore the effects of romidepsin ( FK228) , a novel histone deacetylase inhibitor, on the effector and regulatory T cells in vitro.METHODS:As the reactive cells, lymphocytes, CD4 +T cells and CD8 +T cells were labelled with CFSE, and stimulated with anti-CD3 and anti-CD28 mAbs in the presence and absence of different levels of romidepsin (experimental group and positive control group), or PBS (placebo group).After 72 h, the proliferation of the cells was detected in different groups.The lymphocytes were stimulated with anti-CD3 and anti-CD28 mAbs in the pres-ence and absence of different levels of romidepsin ( experimental group and positive control group ) , or PBS ( placebo group) .After 72 h, the percentage of CD4+Foxp3 +T cells and the levels of related cytokines were detected in different groups.RESULTS:The proliferation of CFSE-labelled lymphocytes, CD4 +T cells and CD8+T cells triggered by anti-CD3 and anti-CD28 mAbs all were inhibited when cultured with romidepsin at concentrations of 1 μmol/L, 3 μmol/L and 5 μmol/L in a dose-dependent manner (P<0.05).Compared with placebo group, in the presence of anti-CD3 and anti-CD28 mAbs, 1 μmol/L romidepsin did not increase the percentage of CD4 +Foxp3 +T cells ( P>0.05 ) .When cultured with romidepsin at concentrations of 3 μmol/L and 5 μmol/L, the percentage of CD4 + Foxp3 +T cells was enhanced markedly ( P<0.05) .The levels of IL-10 and TNF-αin the supernatant were markedly increased in positive control group
and 3 experimental groups ( P<0.05 ) , and the levels of cytokines in different experimental groups were gradually de-creased with the elevation of FK228 concentration (P<0.05).The level of TGF-βwas slightly increased in positive con-trol group with no significant difference compared with placebo group (P>0.05).With the increase in the concentration of FK228 in different experimental groups, the TGF-βlevel was increased in a dose-dependent manner and there were signifi-cant differences in the 3 experimental groups.Meanwhile, significant differences existed between experimental groups and placebo group and between experimental groups and positive control group (P<0.05).CONCLUSION:Romidepsin in-hibits the proliferation of CD4 +and CD8 +effector T cells and increases the percentage of CD4 +Foxp3 +regulatory T cells. It may be related to the increased level of TGF-β, but independent of IL-10.

Objective To assess the effect of Ambroxol combined with Naloxone on respiratory insufficiency on premature infants. Methods A total of 168 premature infants treated from 2006 to 2009 in our hospital were divided into two groups randomly. Additionally to the conventional treatment, Ambroxol (5 mg pertime) combined with Naloxone (0.1mg pertime) intravenously injected 2 to 3 times one day for 3 to 5 days in the treatment group(86 cases). The control group (82 cases) was treated by routine Aminophylline injection,3- 5 mg/kg pertime, 2 to 3 times one day. Respiratory rates, regulately or not,breathholding, cyanosis and apnea,mechanically ventilated time,time of oxygen inhaling, time of hospital stay and the overall response rate were measured. Results The overall response rate was 80% (69/86) in the treatment group and 56% (46/82)in the control group, with significant difference between the two groups ( P ＜ 0. 05 ). Mechanically ventilated time,time of oxygen inhaling and hospital stay in the treatment group was (110.5±45.1) hrs, (169.3±58.6) hrs and ( 12±3) days, which were all significantly lower than that in the control group ( 181.1±81.2) hrs, (236.6±87.8)hrs and (16±5)days,respectively(P＜0.01 or 0.05). Conclusion Ambroxol and Naloxobe additional to conventional treatment could improve the respiratory function in premature infants with respiratory insufficiency safely with less side effects. Moreover,breathing machine could be removed to some extent,which made it more applicable in primary hospitals.