Berberine is a taste of traditional Chinese medicine.With the progress of research on pharmacology and mechanism of drug action of extensive research,the new mechanism of berberine was revealed through a series of clinical trials,the clinical indications continue to expand,and berberine has a therapeutic effect in digestive and endocrine system disease.The current study confirmed that berberine not only has the broad-spectrum antibacterial activity,but also plays a strong anti-inflammatory and immune regulatory role,which makes more and more research began to concern its application value in the diseases of respiratory system.The strong anti-inflammatory effect of berberine cau effectively relieve the airway inflammation of patients who are suffering from chronic obstructive pulmonary,and the broad-spectrum antibacterial effect brought hope for the treatment of tuberculosis and pneumonia.The subsequent animal and clinical studies have confirmed that berberine can effectively improve the symptoms of respiratory system diseases,and play a role in the treatment.

Objective To research pacemaker cells outside conductive system of rat and mouse heart. Methods Paraffin and frozen serial sections (stained with HE, Masson, cholinesterase technique), and ultrastructural sections, from atrial tissue of 10 rats and 15 mice were observed by light and electron microscopy to find latent pacemaker-like cells. Results Some round or elliptical cells were scattered and irregularly detected in right and left atrial tissue of rat and mouse. Their nuclei were larger, plasma was clearer and cellular organelles were fewer than those of working myocyte. These cells were similar to pacemaker cells in sinoatrial node.Conclusion There are some pacemaker-like cells in right and left atrial tissue of rat and mouse. The finding can provide a morphological basis for ectopic beat of atrium.

Replacement therapy is the most effective method for the treatment of end-stage liver disease,and decellularized liver bioscaffold broadens the research field of the replacement therapy.The present liver bioscaffold preparation is to perfuse chemical reagents (detergents,enzymes,et al) into the vascular structure of the liver under certain physical conditions,so as to remove cellular components and retain extracellular matrix and microvascular structure.Cells were reseeded into the decellularized liver scaffold to obtain the recellularized liver,which can be cultured and evaluated in vitro or in vivo by observing the adhesion of seeded cells,detecting the synthesis and secretion of the recellularized liver.Currently,the selection of seed cells,recellularization protocol and recellularized liver transplantation are still under exploration.In this review,the preparation,evaluation,detection and application of the decellularized liver bioscaffold are introduced for the further experimental study and clinical research.

Objective To evaluate the curative effect and indications of medical ozone for the treatment of lumbar disc herniation.Methods 10~20 ml(35~45 ug/ml) medical ozone was injected into the lumbar disc percutaneously each case. The patients were followed up for 6~12 months. Results 60 cases (66 lumbar discs) with lumbar disc herniation were treated with the injection of medical ozone. The total effective rate was 96.67% , excellent and good rate was 76%.The efficacy in herniation to be 30% of vertebral canal diameter and lumbardisc bulging group. Conclusion The treatment of lumbar disc herniation with medical ozone is simple,effective and no complications.

The salivary glands were exposed and isolated from the larvae of Simulium quinquestriatum and stained in carbol fuchsin, squashed between slide and coverslide. Slides were examined and photographed under microscope to measure the polytene chromosomes. Systematic analysis was made. Results indicated that the number of the polytene chromosomes of both isolates is three. The main characteristic chromosomal structures are homologized. Only the banding types of ⅡL are different.

Objective To investigate the prevalence and related factors of peptic ulcer (PU) and Helicobacter pylori (Hp) infection in military men. Methods A stratified multi-stage cluster randomly sampling method was used. A questionary survey was conducted in 6 160 soldiers garrisoned at south China, inclding: PU symptoms and risk factors questionnaires; gastroscopy and Hp detection. Results Incidence of PU correlative symptoms (Sc≥5) in soldiers was 18.60%. The prevalence of PU was 12.78%.Higher incidence of PU was found among the soldiers who enlisted for less than 1 year, or were from south China, or served in armored troops. The prevalence of Hp infection was 62.67%. Hp infection (OR4.60), smoking (OR3.8), sustained intense training (OR4.3), fighting vehicle driving (OR6.0) and frequent mental intension (OR3.7) seemed to be the main risk factors of PU. While hot food, milk and whether or not the only child in a family showed no relation with PU incidence. Conclusion Higher prevalence of PU and Hp infection existed in service men. For soldiers, PU incidence is closely associated with Hp infection, military training, mental factors, and also related to soldiers′ enlisted period, arm of the service and the region where the soldiers come from.

Objective To investigate the differences of connexin 43(Cx43)expression between adult and infant heart. Methods By using immunohistochemistry to observe the expression of Cx43. Results 1.The expression of Cx43 had a punclate distribution in cytoplasm and over the entire surface of the cardiocyte,and a few located at intercalated disk of atrial and ventricular myocardium in the infant heart.2.Cx43 positive granules distributed irregularly in cell side surface and cytoplasm as well as intercalated disk in adult atrium.Cx43 immunolabelling of adult ventricular myocardium was typically confined to the site of intercalated disk.3.The results of image analyzer showed that the amount of connexin43 expression was lower in the atrium than that of the ventricle in infant heart and atrium bigger than ventricle in adult heart.The expression of Cx43 was less in adult heart than that of infant heart.Conclusion The expression of Cx43 was mainly over the entire surface of the myocardium in infant heart.There were expression differences of Cx43 in human ventricular and atrial myocytes.The amount of Cx43 expression was higher in ventricle than that of atrium in infant heart and atrium bigger than ventricle in adult heart.It was less in adult heart than that of infant.It showed that the expression of Cx43 in human heart existed a developmental differences.

Objective To investigate MRI signal features and MRI appearances of patients with advanced stage in polyacrylamide hydrophilic gel injection for facial plasty.Methods In this study,MRI of 11 cases with 23 polyacrylamide hydrophilic gel injection of facial plasty for 6 to 10 years were retrospectively reviewed.All images were acquired with GE 3.0T MR imaging unit.MR sequences,including FSE T1WI,FSE FS T2WI,and STIR were applied with 8-channel brain coil.MRIs sliced through the maxillofacial region in the transverse,coronal and sagittal planes.Results In 11 cases of 23 polyacrylamide hydrophilic gel injection,there were different degrees of capsule rupture and induration in 6 polyacrylamide hydrophilic gel injection,and the images showed sporadic callosities such as subcutaneous nodules and nodules in glands or muscles ; hydrogel migration in 8 polyacrylamide hydrophilic gel injection.Secondary deformity occured in 80 ％ cases,in which the most cases were induced by hydrogel migration.Conclusions Magnetic resonance imaging can make clear of the type of rupture and the distribution leakage of polyacrylamide hydrophilic gel for facial plasty and it is an ideal approach for advanced patients with polyacrylamide hydrophilic gel injection for follow-up.

Objective To develop an apparatus to measure pulse magnetic field. Methods Single chip computer and Hall-effect integrated circuit was utilized to measure the magnetic induction. The correlative hardware and software flow were given. The measuring range of apparatus was 0-50mT. Results The circuit was simple and the performance was safe and credible. The measuring precision was high. Conclusion It can be used for some kinds of magnetic induction measurement.

Objective To investigate of the biological effects of the extremely low frequency electromagnetic fields (ELFEMF),and to explore the effect of rectangular ELFEMF performance parameters on rat fetal skull osteoblast. Methods Different amplitude, frequency and duty cycle of rectangular ELFEMF were used on rat fetal skull osteoblast and the proliferation and differentiation of osteoblast were examined. Results There existed action windows of amplitude and frequency with regard to the biological effect of rectangular ELFEMF. The 15 Hz, 5 mT and 15% duty cycle could significantly promote the proliferation and decrease differentiation of the cells. Conclusion The proliferation and differentiation of osteoblast could be affected by rectangular ELFEMF characterized by its amplitude, frequency and duty cycle. This study indicated that rectangular ELFEMF of 15 Hz, 5 mT and 15% duty cycle was suitable for the proliferation and differentiation of osteoblast in vitro.