Gastric cancer is a malignant tumor with high incidence and mortality in China.Invasion and metastasis of carcinotissue is one of the main reasons for the death of patients with gastric cancer.The mechanism of gastric cancer invasion and metastasis is very complicated,containing several immunomolecules and genes.Up-or down-regulated expression of these molecules in gastric cancer invasion and metastasis means that these immunomolecules and genes play some important roles in generation and progression of gastric cancer.In this review,we introduced the structure and function of these immunomolecules and genes,and their action during the course of gastric cancer invasion and metastasis.

The source, super-antioxidant activity, biological safety and application in medicine, health food and animal feed industry of natural astaxanthin are reviewed in present paper. The industrialization prospect of astaxanthin in China is also proposed.

Objective To investigate the expression and significance of cyclin E in rectum carcinoma.Methods The expression of cyclin E was examined by immunohistochemical techniques in 42 cases of rectum carcinoma.Laboratory data were then analyzed statistically together with the related clinical and pathological data.Results The positive expression rate of cyclin E in rectum carcinoma was 66.7％(28/42).There was no significant association between cyclin E and gender,age,histological grade,pTNM stage,metastasis of lymph node (P ＞ 0.05).Conclusions The expression of cyclin E in rectum carcinoma is higher,and it may show highly associated with the occurrence and development of the rectum carcinoma.Cyclin E has no significant association with age,gender,histological grade,pTNM stage,metastasis of lymph

Objective To discuss the clinical effect and safety of the unilateral C2 pedicle screw-plate(C2PSP)combined with contralateral C2 laminar screw-rod(C2LSR)for atlanto-axial joint and occipital cervical fixation.Methods 11 patients were treated by internal fixation with pedicle screw plate system combined with contralateral C2 laminar screw rod system.The cause of atlantoaxial instability was fresh type Ⅱ odontoid fracture in 5 patients,old odontoid fracture in 3 patients,occipitalization mal-formation in 2 patients,atlantoaxial instability in 2 patients.6 patients had small pedicle of the vertebral arch,2 patients had a high-riding vertebral artery(VA),2 patients had an asymmetrical VA,1 patient re-ceived a revision operation.9 patients performed C1-2 fixation,2 patients performed occipito-cervical fixa-tion.1 patient of irreducible atlantoaxial dislocation also performed anterior atlantoaxial joint release before posterior Fixation.All patients performed bone grafting to achieve a further occipito-cervical fusion or at-lantoaxial fusion.Before and after the operation,the neurologic function was scored according JOA scale. The X-ray and CT-scan also performed at regular intervals to evaluate cervical alignment and the fusion. Results All cases were complete reposition without vertebral artery,spinal cord or never root injuried.All patients were followed up for 6-32 months ( mean 16 months ),clinical symptom were improved visibly. The mean postoperative JOA scores was 14.9(13-16).The postoperative JOA score improvement rate was 76%-92%,and its mean value was 83%.All patients achieved bone fusion approved by CT scan after six months of operation.The reduction maintained well and the internal fixation was well positioned.Conclu-sion It will be an effective and safe way to achieve atlantoaxial fusion by posterior unilateral C2 pedicle screwing combined with contralateral C2 laminar screwing,but this study had not including clinical com-parative study.

Objective To explore factors affecting cardiopulmonary and cerebral resuscitation in patients with cardiac arrest. Methods Logistic regression analysis was applied to analyze the factors affecting the survival rate of patients with cardiac arrest after cardiopulmonary resuscitation and return of spontaneous circulation (ROSC). Results 6 factors were related to the patient prognosis, including where the cardiac arrest took place, when the cardiopulmonary resuscitation (CPR) began, intervals of ROSC, pupils size, PRM (postresuscitation MODS) and age. multivariant analysis showed that PRM was the primary factor affecting the patients prognosis. Conclusion The time of taking CPR was the crucial factor affecting CPR success. PRM was an independent factor affecting the survival rate of the patients after ROSC. Rapid and effective resuscitation in the first place is the key to basic life support. More emphasis should be placed on the evaluation, monitoring and protection of the function of important organs after successful CPR.

Objective To explore the roles of curcumin in regulating high level O6-methylguanine-DNA methyltransferase (MGMT) expression and the chemosensitivity of gliomas cells to temozolomide (TMZ) in malignant gliomas.Methods The expressions of MGMT were detected in C6 and U87 cell lines and primary malignant gliomas cell by qRT-PCR.The cell proliferation was analyzed after added in signal curcumin,signal TMZ,curcumin combined with TMZ in cells by CCK-8 assay.The cell apoptosis rate was detected by flow cytometry.Results Compared with single curcumin (C6:0.64 ±0.03;U87:0.63 ±0.06;primary cell:0.51 ±0.07) and single TMZ (C6:0.53 ±0.06;U87:0.51 ±0.04;primary cell:0.79 ±0.03),curcumin combined with TMZ (C6:0.14 ±0.01;U87:0.12 ±0.03;primary cell:0.29 ±0.02) could significantly reduce the expression of MGMT in C6,U87 cell lines and primary cell line (C6:F=23.675,P=0.006;U87:F=29.021,P=0.001;primary cell:F=25.534,P=0.001).The combination of curcumin and TMZ could significantly inhibit the cells proliferation,decrease the halfinhibition concentration (IC5o) value (C6:F=6.731,P=0.012;U87:F=17.321,P=0.008;primary cell:F=18.857,P =0.007).The apoptosis rate of the cells was significantly increased after the combined action of curcumin and TMZ (C5:F=25.871,P=0.001;U87:F=6.847,P=0.009;primary cell:F=36.641,P=0.000).Conclusion Synergistic effect of curcumin and TMZ can reduce the expression of MGMT and increase the chemosensitivity of TMZ to malignant glioma,and provide new strategy and methods for the treatment of malignant glioma.

The salivary glands were exposed and isolated from the larvae of Simulium quinquestriatum and stained in carbol fuchsin, squashed between slide and coverslide. Slides were examined and photographed under microscope to measure the polytene chromosomes. Systematic analysis was made. Results indicated that the number of the polytene chromosomes of both isolates is three. The main characteristic chromosomal structures are homologized. Only the banding types of ⅡL are different.

Objective To explore the correlation between the increasing of blood glucose and cytokines in malignant tumor patients after chemotherapy.Methods According to the blood glucose after chemotherapy,155 cases of malignant tumor patient were divided into 3 groups:euglycemia group,IGT(impaired glucose tolerance)group and DM(diabetes mellitus)group.The variance of cytokines in the 3 groups were analyzed.Results Compared with pre-chemotherapy,IL-6,TNF-αincreased greatly in IGT group and DM group,while IL-10 decreased greatly.The statistical difference were obvious compared with euglycemia group.Conclusion There are close correlation between the increasing of blood glucose and cytokines in malignant tumor patients after chemotherapy.

Objective To screen the formulation for tanshinone self-emulsifying drug delivery system (SEDDS) and evaluate its stability.Methods The optimal tanshinone SEDDS formulation was established through solubility experiment,emulsion examination,fully emulsified time,droplet size determination,and pseudo-ternary diagram drawing.The content and stability were evaluated by HPLC assay under the condition of illumination,high and low temperature.Results The oil phase,surfactant and co-surfactant in the optimal tanshinone SEDDS formulation were ethyl oleate,TX10,Tween 80,and isopropyl alcohol (60∶84∶21∶35).Conclusion The acquired formulation of tanshinone SEDDS is stable in the dark and room temperature.

Objective To compare the efficiencies of Fenwal CS-3000 plus and Cobe Spectra blood cell separators in collecting peripheral blood stem cells. Methods Thirty-eight stem cell apheresis procedures were performed on 31 patients using Fenwal CS-3000 plus cell separator and 65 procedures performed on 45 patients using Cobe Spectra cell separator. The white blood cell counts and the percentages of mononuclear cells and CD 34 positive cells in the apheresis products of the two groups were compared. Results The WBC counts of the two groups had no significant difference. The mononuclear cell percentage was significantly higher with Fenwal CS-3000 plus than with Cobe Spectra. The mononuclear cell percentage declined with increasing WBC count when using Cobe Spectra. There was no significant difference between CD 34 positive cell percentages of the two groups. There were no significant correlations between the WBC count and the percent of CD 34 cells, between the percent of mononuclear and the percent of CD 34 cells of the apheresis products. Conclusion There is no difference between the collection efficiencies of the two blood cell separators in the collection of peripheral blood stem cells.