Objective To investigated the relationship between serum matrix metalloproteinase-9(MMP-9) and carotid atherosclerosis(AS) in type 2 diabetes mellitus(T2DM).Methods A total of 93 patients with T2DM were recruited to our study.The intima-media thickness(IMT) and plaques of carotid artery were measured.These patients were divided into 3 groups according to their IMT values: diabetes mellitus(DM) group(n=32),carotid artery intima thicken group(n=31) and carotid artery intima plaque group(n=30).At the same time,30 healthy individuals were selected as control.Serum level of MMP-9 were determined and analyzed.Results The serum MMP-9 in DM group was significantly higher than that in healthy controls([550.26±269.28]μg/L vs.[359.70±215.62]μg/L,t=2.23,P＜0.05).The serum MMP-9 level of intima thicken group(712.15±340.47)μg/L was significantly higher than that in healthy controls(t=4.53,P＜0.01) and DM group(t=2.40,P＜0.05).The serum MMP-9 level of plaque group([889.08±247.80]μg/L) was even more significantly higher than DM group(t=4.89,P＜0.01),IMT group(t=2.53,P＜0.05) and healthy controls(t=7.01,P＜0.01).Conclusion The severity of carotid atherosclerosis in T2DM is closely associated with the serum MMP-9 level.

Using HB55 McAb, we detected HLA-DR antigens on Daudi, H18, PBL and K562 cells by dot-ELISA. Results corresponding to situation of the antigen expressions on various cell lines were obtained: colour reaction in Daudi, H18 cells and PBL was positive in different degree and K562 sample presented negative reaction. Chromatogram peaks were printed by Dual-Wavelength Flying-Spot scanner CS-9000 (SHIMADZU). Areas and high values of peaks, meaning size and colour of spot respectively, were increased with cell concentration. Both cell concentration and volume of cell suspension did not influence the diffusion of sample. An analysis for the antigen expression on cell surface and its dynamic change can be supplied by the technique.The study on quantitative assay of MHC class Ⅱ antigens on cell surface are still going on.

Objective To study the effect of short-term inte ns ive insulin treatment on the pancreatic B-cell function in type 2 diabetic mell itus (T2DM).Methods Insulin and C-peptide serum levels were measured befor e and after intensive insulin treatment in T2DM by chemical luminescence immunoa ssay.Results The levels of insulin and C-peptide were increased at 0、30、60、120、180min in OGTT in T2DM after insulin intensive treatment.Conclusion Short-term intensive insulin treatment can partiall y improve the B-cell function of T2DM with lower function and made it rest.

Objective To study the levels of serum resistin and sICAM-1 in diffferent glucose tolerance status.Methods Fasting serum resistin and sICAM-1 concentration were measured by enzyme immunoassay in 52 cases suffering from T2DM,18 cases with glucose tolerance impaired and 30 cases with normal glucose tolerance.Meanwhile,fasting blood glucose,insulin and lipids were determined.HOMA-IR was calculated.Results Fasting serum resistin concentration (ng/mL) was higher in IGT than that in those patients with T2DM and NGT (P

Objective To study the clinicopathological features of C3 glomerulonephritis and the associations between plasma complement fragments level and clinical manifestations.Methods The clinical and pathological data of the 12 patients with C3 glomerulonephritis in our division from January 1999 to June 2010 were analyzed retrospectively.Concentrations of plasma factor B,Ba,C3,C3a,C4a and C5a were detected by commercial available sandwich ELISA kits on the day of renal biopsy.Results Ten of the 12 patients were C3 glomerulonephritis without MPGN,and the rest 2 were C3 glomerulonephritis with MPGN.All the patients presented proteinuria.Two of the 10 C3 glomerulonephritis patients without MPGN presented nephrotic range proteinuria,6 with microhematuria,1 with gross hematuria,and 2 with renal insufficiency.One of the 2 C3 glomerulonephritis patients with MPGN presented nephrotic range proteinuria,accompanied by microhematuria,hypertension and renal insufficiency.The other patient showed moderate proteinuria with normal renal function.Most of C3 glomerulonephritis patients without MPGN showed mild mesangial proliferative glomerulonephritis,and 4/10 patients had various degree cresentic formation.One C3 glomerulonephritis patient with MPGN had 47.1％ cresentic formation.The concentration of plasma C3 in C3 glomerulonephritis patients was normal,while the plasma factor B was significantly decreased,and the concentrations of plasma Ba,C3a,C4a and C5a were significantly elevated.The concentration of plasma Ba was positively correlated with the proteinuria level,while the concentrations of plasma C3a,C4a and C5a were not correlated with the levels of proteinuria or plasma creatinine.Conclusions Majority of these 12 patients were C3 glomerulonephritis without MPGN,manifests as nephritic syndrome clinically and mild mesangial proliferative glomerulonephritis histopathologically.Complement activation via alternative pathway may play an important role in the pathogenesis of C3 glomerulonephritis.

Robot-assisted surgery is becoming a widely popular technology and now entering total knee replacement. The development of total knee replacement is introduced in this paper with the emphasis on femur positioning technologies. The experiments and error analysis of robot-assisted surgical system for total knee replacement demonstrate that femur positioning technologies have a high precision and thus deserve to be popularized theoretically and practically.

Objective To observe the change of endothelium-dependent vasodilatation function and to explore its mechanism in insulin-resistant (IR) rats induced by high fat feed. Methods (1) IR rat model was established by high fat feed for 4 weeks and IR was evaluated by glucose infusion rate (GIR) of euglycemic hyperinsulinemic clamp technique. (2) Acetylcholine (Ach)-dependent vasodilation response and nitric oxide synthase (NOS)-nitric oxide (NO)-cGMP function status were observed in isolated aorta of rats. Results (1) GIR was obviously lower in high fat feed group than that in routine feed group (P

Objective To investigate the use of salvianolate injection for inpatients in our hospital; To primarily evaluate its rationality and security in clinic.Methods Medical records using the salvianolate injection from December 2013 to March 2014 were surveyed statistically and analyzed in respect of patient gender and age, department, disease diagnosis, usage and dosage, solvent types, treatment course, medicine combination, occurence of ADR, etc.Results Totally 600 cases of inpatients receiving salvianolate injection were collected, about 69.33% consistent with the indication, 67.00% with a correct single dosage, and 52.50% with a correct treatment course. In the clinical application of salvianolate injection, a high rate of unreasonable usage was found in indication, single dosage, treatment course and medicine combination.ConclusionBecause the problems still existed in the use of salvianolate injection, it should be used strictly according to the medicine instructions and General Principles for Clinical Application of Traditional Chinese Medicine Injection to ensure clinical medication safety.

Objective To Summarize experiences in the treatment of serious injury of pancreas using damage control laparotomy. Methods Retrospectively analyze the choice of surgical method and follow-up treatments on 38 patients cured by DCL,who were in serious injury of pancreas. Results 12 cases(32%) were stanched, surgical debrided and drainage quickly,6 cases(16%) were treated with improved Cogbill operation,and 20 cases(S3% ) were treated with pancreateduodenectomy without reconstruction of enteron. After recovery in SICU, all patients were treated with deterministic operation again. Totally 4 cases(11%) died,with no relation with surgical operation,and 34 cases (89%) were cured. Pancreatic fistula occurred in 4 patients(11% ) ,all of which have been cured with conservative treatment. Conclusion Patients with serious injury of pancreas in accordance with DCL, should choose proper method and take fractionated operation,based on different places and extent of the injury of pancreas.

Objective To investigate the levels of mieropartieles originated from platelet (PMP),endothelium (EMP),and tissue factor-bearing microparticles (TF+ MP) in diabetes mellitus and to analyze its relationship with diabetic angiopathy.Methods PMP,EMP or TF+ MP were measured in 106 cases of diabetes mellitus and 50 controls by flow eytometry.The differences of EMP between groups of diabetic macrovascular disease and diabetic microvascular disease were determined.Results The levels of EMP in diabetic patients were higher than that in the control(164.20±128.88 vs 63.81±40.84,P<0.05).Diabetic cases with complication showed higher expression level of EMP than those without complications(184.12±152.77,188.21±149.55 vs 138.53±99.87,both P<0.05).However,no distinct increase was observed in PMP and TF+ MP level in diabetes groups compared with control group.Conclusions Endothelial dysfunction,may contribute to the increased level of EMP in patients with diabetes,especially those complicated with vascular diseases.EMP level may be used to evaluate the status of endothelial function and the development of diabetic angiopathy.