Objective:To investigate the protective effect of punicalagin in LPS induced ALI.Methods: ALI mice model was induced by intraperitoneal injection of LSP(20 mg/kg bodyweight) to induce acute lung injury in mice.A total of 60 mice were divided into 6 groups,10 rats in each group:normal control group,LPS group,LPS+punicalagin group (10,20,40 mg/kg bodyweight),LPS+dexamethasone group.2 h before LPS injection,punicalagin and dexamethasone were intraperitoneal injection in mice,and then by intra-peritoneal injection of a lethal dose of LPS, normal control group mice were given intraperitoneal injection of an equal volume of PBS.HE staining,observed the lung tissue of mice in the different treatment conditions of the pathological changes and the degree of lung injury,weighted the wet lung and dry lung of the mice respectively, observed degree of pulmonary edema in mice, inspect MPO activity of lung homogenate, analyzed the activation and infiltration of polymorphonuclear, counted the total number of cells and the number of inflammatory cells in alveolar lavage fluid, detected the concentration of inflammatory cytokines in bronchoalveolar lavage fluid with ELISA,and analyzed the impact of punicalagin on inflammation of lung tissue.Results: HE staining of lung tissue in mice and found that the LPS-induced lung inflammatory cell infiltration, interstitial edema and pulmonary parenchymal damage were significantly reduced.Different dose of punicalagin could significantly reduce the lung wet/dry lung weight ratio of ALI in mice ( P<0.05).Punicalagin ALI could reduce lung tissue caused exudation,edema.The different doses of punicalagin could significantly reduce the inflammatory cells in bronchoalveolar lavage fluid,the sum of the number of neutrophils and macrophages (P<0.05).Punicalagin could significantly reduce the concentration in the bronchoalveolar lavage cytokines IL-6,IL-12,TNF-αand IL-1β(P<0.05).The MPO activity was detected that punicalagin reduce neutrophil infiltration induced by LPS in the lung tissue.Conclusion: Punicalagin has protective effects on LPS induced acute lung injury.

Objective Vascular endothelial growth factor( VEGF) is the key to regulat vascularization, and plays an important role in the development of acute myeloid leukemia. With the understanding of anti?tumor angiopoiesis, researchers take VEGF and its receptors as targets, continuously exploring the new drug. We summarized the biological characteristics of VEGF, vascular?targeted therapy and the clinical application of drugs.

Objective To probe the methods of experimental research for the drug activities of the rat prostates. Methods With androgen and Staphylococcus aureus injected into the prostates, the bacterial prostatitis (BP) models and benign prostatic hyperplasia with bacterial prostatitis (BHP-BP) models were developed. The animals were fed with tetracycline and then the drug activities in the serum and the prostatic tissues, the quantities of the bacteria and the histopathlogical changes in the prostateic tissues were separately detected in different time. ResultsAfter ten days, the histopathlogical changes of the benign hyperplasia and mesenchyma were found in the prostatic tissue from BHP animals and acute suppurative inflammation were dicegnosed in prostatic tissue from the BP group after infected for two days. The drug activities of 22.44 mg/g in BP group and 20.26 mg/g in BPH-BP group were found until 3.5～4 hours and the values were higher than MBC of the tetracycline and the antibacterial activity of the sera at the same time. After 8 to 11 days of treating, the bacteria would not be isolated from the prostates of the animals with BP or BHP-BP. Stopping treatment a week 11 days after treating acute or chronic inflammation histopathlogical change did not be found in the prostates of animals with BP. 30 CFU of bacteria could be found in per gram tissue of prostate and both of the acute and chronic suppurative inflammation could be found in the BP group without treatment at the 28 days afterinfected.Conclusion The tetracycline activities in the prostates of rats with BP or BPH-BPis same as or even higher than that of serum and the MBC of the drug. The quantity of sensitive bacteria in prostates will be diminished and disappeared during treatment.

Objective To understand the rpoB gene mutation in M.tuberculosis isolates,and to evaluate their clinical value.Method 335 clinical isolates of mycobacterium tuberculosis(109 isolates drug susceptible,246 isolates rifampin-resistance or multidrug resistance including rifampin) were detected using polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP).Results SSCP pattern of reference mycobacterium tuberculosis H37Rv as control,no mutation was found in rifampin-suscepitible 109 strains.SSCP patterns of 225/246 rifampin resistant clinical isolates were different from the normal control.The sensitivity was 91 5%.31 resistant isolates,included 25 abnormal isolates and 6 normal isolates of SCCP were identified.Sequencing showed 29 isolates had rpoB gene mutations and 3 isolates were not found rpoB gene mutations.The 3 most frequent rpoB gene mutation situs were Leu-531(19 isolates.TCGTTG) and His-526(7 isolates,CACTAC) and Asp-516(3 isolates,GACGTC).Conclusions The results confirm that rpoB gene mutation is the most important mechanism in rifampin resistant tuberculosis mutation situs are 531 tryptophan and 526 histidine;respectively.It is feasible that using PCR-SSCP to detect drug resistance in mycobacterium tuberculosis.

Objective To construct a cDNA library from human nasopharyngeal carcinoma cell HNE 2.Methods The total RNA was separated from human NPC(nasopharyngeal carcinoma)cell HNE 2 and the mRNA was isolated from the total RNA by MagneSphere technique,then the first-strand cDNA was synthesized with oligo(dT) primer containing sfiI site while the double-strand cDNA was amplified through LD-PCR(Long-distance PCR) by SMART technique.The double-strand cDNA was digested by sfiI(IA &IB)restriction enzyme before cDNA size fractionation ,the double-strand cDNA fractionated was ligated into the ?TripIEx2 vector and then was packaged in vitro.Results The unamplified human NPC cell HNE 2 cDNA library consists of 0 78?10 6 independent clones,and the percentage of recombinant clones was more than 96%.The titer of the amplified cDNA library was 1 02?10 9 pfu/ml and the average insert of the recombinants was 1 2kb.Conclusions The quality of the constructed human NPC cell HNE 2 cDNA library is excellent and helpful to screen NPC specific-antigen.

OBJECTIVE: To investigate the characteristics of psychiatric comorbidity in pharyngitis patients. METHOD: The psychological investigation and evaluation were done in 100 pharyngitis patients by using self-reporting inventory 90 (SCL-90). All the results were compared with healthy Chinese population, including factors of gender, age, course of disease and education level, using rank-sum test analysis, SPSS 19.0. RESULT: Mild and moderate levels of psychosis were respectively noted in 65% and 14%, with high score of somatization (P < 0.05), obsession (P < 0.05), interpersonal sensitivity (P < 0.05), anxiety (P < 0.05) and other (sleep, diet, P < 0.05) compared with control group. The factor scores of obsession (P < 0.05), anxiety (P < 0.05) in male group were higher than female group. The factor scores of somatization (P < 0.05) and obsession (P < 0.05) in junior group were higher than senior group. The factor scores of somatization (P < 0.05), interpersonal sensitivity (P < 0.05) and anxiety (P < 0.05) in long course group were higher than short course group. The factor score of interpersonal sensitivity (P < 0.05) in low educated group was higher than highly educated group. CONCLUSION Psychiatric disorders are prevalent in pharyngitis patients, especially upon the factors of somatization, obsession, interpersonal sensitivity, anxiety and other (sleep, diet). Male, junior, long course patients and low educated are in high risk group.
Anxiety ; complications ; Asian Continental Ancestry Group ; Factor Analysis, Statistical ; Female ; Humans ; Male ; Mental Disorders ; complications ; Personality Inventory ; Pharyngitis ; complications ; psychology ; Prevalence

Objective Establishment and characterization of healthy donor's ??T cell clones.Methods ??T cells were cloned by limiting dilution after positive sorting with 60Co irradiated allogeneic PBMC as feeder cells.Flow cytometry analysis and molecular biology technique were then used to identify ??T cell clones.MTT assay was used to verify their proliferation after incubated with epitope peptides recognized by ??T cells.Results A ??T cell clone had been established.The subtype of this clone was V ?9 V ?2 without expression of CD4 and CD8.Further studies indicated that epitope peptide EP6 could not only specifically bind to ??T cell clone but also trigger the proliferation of ??T cell clone.Conclusion A healthy donor's ??T cell clone was successfully established,which laid a solid foundation for further study on ??T cells.

The change of Dietary pattern matched to a change in diseases spectrum of Chinese people in recent years. For improving the function of clinical nutrition branch in hospitals, in accordance with the change in the spectrum and patients' characters, we need to adjust the clinical nutrition treatments individually, to enhance the health education and consultation, to set new dietary pattern for people rationally, and to carry out the scientific research in the field of the relation between concerned chemical elements of organical foods and human health status in general, in order to reach the advanced hospital standards.

Requirement for Medical English teaching in physical medicine physicians training has been on the agenda to fit the new condition of globalization. According to the development of physical medicine in China and students English level, courses of medical English were set to match the requirements of both scientific research and clinic works. We try to improve students' medical English level through lectures in multimedia classroom and a lot of practical activities after class.

Objective: To prepare and characterize the polyclonal antibody against KIAA0649 and identify the localization and the functional motif of KIAA0649. Methods: Three polypeptides were synthesized based on the bioinformatics analysis of KIAA0649 protein. New Zealand rabbits were immunized with the mixture of the three KIAA0649 peptides coupled with keyhole limpet hemocyanin ( KLH). The titer of the antisera was detected with ELISA. The antisera were purified with immuno-affinity chromatography when the titer reached 1:10~5. Western blot was performed with the purified antisera on the cell lysates of U2OS cells transfected with either Flag-KIAA0649 or KIAA0649-targeting siRNA. Immunofluorescence was performed with the purified antisera and anti-Flag antibody on the cells transfected with FlagKIAA0649. A series of Flag-K1AA0649 deletion mutants was constructed by PCR cloning. The cellular compartmentation of full-length Flag-KIAA0649 and its deletion mutants were analyzed with immunofluorescence. Results: The results of Western blot and immunofluorescence demonstrated that the antisera from the KIAA0649 polypeptides-immunized rabbits specifically recognized endogenous and exogenous KIAA0649. The full-length Flag-K1AA0649 displayed specific nuclear foci. The Flag-KIAA0649 deletion mutant containing PENF motif showed the same nuclear foci as the full length of Flag-KJAA0649, suggesting that the PENF motif could be the minimum functional motif of KIAA0649. Conclusion: We have obtained anti-KIAA0649 polyclonal antibody which will be useful for further investigation. The PENF motifcould be the minimum functional domain of KIAA0649.