Objective To explore the condition of cortistatin (CST)expression in human renal tissue and the changes in the level of CST in IgA nephropathy (IgAN)of different degrees.Methods Ten tumor adjacent normal renal tissue samples were collected.The mRNA and protein expressions of CST in human renal tissue were detected by reverse transcription-polymerase chain reaction (RT-PCR)and Western blotting,respectively.Immunohistochemisty (IHC)was performed to locate the expression of CST in renal tissue.According to the grading system of Lee et al,IgAN was divided into three groups:grade Ⅰ -Ⅱ (group A),grade Ⅲ -Ⅳ (group B),and grade Ⅴ (group C),and ten renal biopsy tissue samples were collected for each group.IHC was performed to detect the change in the level of CST in normal and IgAN renal tissue of different degrees.The effect of clinical indices on the level of CST in IgAN renal tissue was assessed by multiple linear regression analysis.Results RT-PCR and Western blotting showed that CST was expressed in renal tissue and IHC showed that CST was expressed on renal tubular epithelial cells.In IgAN,the higher the pathological grade was, the higher the expression of CST in renal tubules was.Multiple linear regression analysis showed that the pathological grade was associated with the expression of CST in renal tissue (r =0.875,P <0.01).Conclusion CST may participate in the inflammatory reaction of IgAN pathological injury and exert anti-inflammation effects.

AIM: To investigate the effects of EPA and DHA on oxidative stress of lipopolysaccharide-stimulated rat mesangial cells. METHODS: The glomerular mesangial cells (GMCs) were stimulated by lipopolysaccharide (LPS) and incubated with EPA (10 μmol/L or 100 μmol/L) and DHA (10 μmol/L or 100 μmol/L) for 24 h, 48 h and 72 h. The activity of SOD, GSH-Px and the level of MDA was measured. The protein and mRNA expressions of MCP-1 and TGF-β_1 were detected by immunocytochemistry and real-time PCR method, respectively. RESULTS: The activities of SOD and GSH-Px were decreased and the concentration of MDA was increased when stimulated with LPS. EPA and DHA increased the activities of SOD and GSH-Px and decreased the concentration of MDA significantly. Meanwhile, the protein and mRNA expressions of MCP-1 and TGF-β_1 stimulated by LPS were decreased. DHA was more effective than EPA at the same concentration. CONCLUSION: EPA and DHA enhance the activities of antioxidant enzymes, decrease the concentration of MDA and inhibit the expression of TGF-β_1 and MCP-1, suggesting that the protective effect of EPA and DHA on kidney is related to the antioxidation and the inhibition of TGF-β_1 and MCP-1 expression.

Objective To establishthe sensitivity indexes system for estimating nursing quality which conforms to China's national conditions and have the certain representative among the whole country. Methods After usingStructure-process-outcome as the theoretical framework and surveying the literatures domestic and abroad,a total of 42 nursing management experts from 30 provinces were consulted by the Delphi method,including symposium and two rounds of questionnaire consultation. Results The authority coefficient of the two rounds consultation were 0.89 and 0.91,familiarity coefficient were 0.86 and 0.88 respectively,and the coordination coefficient of expert opinion were 0.20 and 0.25. The sensitivity indexes system for estimating nursing qualityconsists of two level index systems,which were divided into three first level indexes and seven second level indexes. The first level index in-cluded nursing manpower allocation,nursing safety and nursing events,while the second level index includes nurse-inpatient ratio,accuracy rate of identification,the number of medication error events,the number of blood transfusion error events,the number of specimen error events,the number of drug extravasation events and high-risk patient falls (falling bed) incidence. Conclusion The results of this study are reliable. There is a clear definition and interpreta-tion of sensitivity indexes for estimating nursing quality and index monitoring is operable.

Quality management of scientific research is a systematic work,quality control of multicenter nursing research depends on perfect and effective quality management system,measures and procedures,as well as rigorous scientific attitude in project team. To establish and improve quality management system of scientific research,to for-mulate standard quality control procedures,to promote the usage of electronic data acquisition system in multicenter nursing researches,and to improve scientific,standard,and refined quality management of research are effective mea-sures to guarantee quality control of multicenter nursing research. This paper summarized quality control methods for multicenter investigation,to provide more experience for nursing researchers as references.

We designed and applied the electronic data capture system (EDC) in order to strengthen the re-search quality and improve the efficiency of data collection of multi-center nursing research. According to the needs of research,we reasonably designed the EDC structure and functions,including data collection,verification, summary and export. Further improvement was performed after EDC was inputted into using. By using EDC,resear-chers from sub-centers completed the data collection smoothly,and the data quality met with research requirements. The design and application of EDC could effectively guarantee the data quality and improve the research efficiency.

Humans ; Lung Neoplasms ; diagnostic imaging ; pathology ; Tomography, X-Ray Computed

Structured clinical examination (OSCE)is an objective,orderly and organized appraisal frame,which was used broadly in medical and nursing education around the world.However,application of OSCE in the assessment of nurses′clinical competences requires more investigation in China.We reviewed site settings of OSCE application,evaluations of nurses and educators on OSCE and challenges in carrying out OSCE,and expect applications of OSCE in the assessment of hierarchical nurses′competences.

Objective To explore the core competency of new nurses after one year's standardized training,and analyze its influencing factors.Methods A cross-sectional study was conducted. Competency inventory for registered nurse was used to investigate 77 nurses who have been registered nurses for a year of Peking Union Medical College Hospital in August 2015,meanwhile,t-test and analysis of variance were used to compare the core competence between new nurses with different characteristics.Result The total score of core competence of new nurses was (159.55 ±27.16),83.12% of whom were in the middle level of core competency. The dimension of legal and ethical practice scored the highest while critical thinking and research aptitude scored the lowest. Nurses who have rotated to intensive care unit (ICU) scored slightly lower in the dimension of interpersonal relation than those not (P<0.05). New nurses of operating room scored lower in the dimension of teaching-coaching than nurses of other departments (P<0.05).Conclusions The core competency of new nurses are in middle level after one year's standardized training. The self-reported core competency of new nurses provides references for evaluating the training program and developing a hierarchy training program.

Objective To evaluate the effects of standardized management of clinical nursing teachers' on improving satisfaction of nursing students. Methods Nursing students who were practicing in a hospital from July 2013 to May 2015 were recruited in the study by convenience sampling method. Nursing students in 2013 and 2014 academic year were distributed in control group (n=300) and experimental group (n=343) respectively. The clinical nursing teachers in the control group were managed according to the hospital routine, while the clinical nursing teachers in the experimental group received the standardized management intervention. Nursing students' satisfaction questionnaire was used to evaluate the effect of intervention between the two groups. Results The total score of satisfaction questionnaire of nursing students in the experimental group was (99.16±4.42), which improved significantly than the control group (98.60±5.08), and the difference was statistically significant (t=3.094, P=0.002). Conclusions Standardized management of clinical nursing teachers' can effectively improve the quality of clinical teaching and the satisfaction degree of nursing students.

Objective: To evaluate the effect of the horn shaped flap pedicled with the angular artery perforator for midface reconstruction. Methods: From March 2014 to April 2017, twenty patients underwent operations for the reconstruction of midface defect, defect due to trauma, tumors, moles, scar and other soft tissue defects after resection. Various horn shaped flap pedicled with the angular artery perforator, perforator flap was rotated and pushed to repair the defect area.The maximum width of the perforator flap was equal to the width of the defect, and the length is about 2.5 to 3.0 times the length of the defect. Results: All flaps survived.After 1 month to 1 year follow up on 20 cases, the flap is significantly improved in color、shape and function.The patients were satisfied with the final aesthetic and functional results. Conclusions The flap has flexibility and simply with reliable blood supply.The donor sites could be closed directly without skin graft.it is a simple and fast method for the reconstruction of midface defect.