Objective To investigate the effect of Ras protein on the delayed myocardial protection of Lovastatin preconditioning in the ischemic and reperfused rat hearts.Methods 24 Sprague-Dawley male rats were randomly and evenly divided into three groups,model control group,N group and Lovastatin group.Lovastatin group was treated with 15mg Lovastatin per a kilogram once a day,N group treated with the same dose of Lovastatin with Lovastatin group and L-NAME 30mg per a kilogram once a day.Rat models of I/R were established with coronary occlusion 30 minutes and reperfusion 30 minutes of the left anterior descending artery after two-week's administration.The expression of Ras-GTPase protein in the heart tissue was determined and myocardial apoptosis was detected in all groups.Results The number of TUNEL positive myocardial nuclei in L group was remarkably reduced,compared with C group(t=2.32,P＜0.05).Ras-GTPase protein in the heart tissue was significantly inhibited in the L and N group,compared with C group(t=2.25,P＜0.05).Conclusion Lovastatin may have the delayed protective effect on the ischemie and reperfused rat heart,which effect may be correlated with inhibition of RasGTPase protein during the period of ischemia and reperfusion.Its protective effect was not correlated with NO.

Objective To explore the effect of early intervention on myelin-associated glycoprotein (MAG) expression of white matterin neonatal rats with cerebral brain injury caused by intrauterine infection. Methods 17 days or 18 days pregnant Wistar rats were consecutivelyinjected with lipopolysaccharide (LPS) intraperitoneally. 100 neonatal rats born from LPS group were randomly divided into interventiongroup and non-intervention group with 50 in each group. Another 10 pregnant rats were injected with normal saline, 50 neonatal rats born from the the normal saline group were taken as control group. The intervention group received early intervention and rehabilitationtraining. All the groups underwent hanging test and modified BBB test on the 14th, 21st and 28th day. 6 rats in each group were tested by immunohistochemicalstaining to observe the MAG expression in white matter on the 1st, 7th, 14th, 21st, 28th day. Results The scores of hangingtest and modified BBB test were significantly higher in the control group than in the LPS group (P<0.01), and higher in the interventiongroup than in the non-intervention group (P<0.01). The expression of MAG was higher in LPS group than in the control group (P<0.01), innon-intervention group than in the intervention group (P<0.01). Conclusion Early intervention and rehabilitation training can improve themotor function in rats with brain injury and reduce the expression of MAG.

Carcinoembryonic antigen-related adhesion molecules (CEACAMs) belong to the immunoglobulin superfamily.Related studies show that CEACAMs play critical roles in the occurrence and development,and are closely related to the prognosis of lung cancer.Recently,it has made some progresses in the studies by using monoclonal antibodies,single domain antibodies,RNA interference,gene knockout and other technical means targeting different epitopes of CEACAMs in lung cancer,suggesting CEACAMs may become new diagnostic biomarkers and therapeutic targets of lung cancer.

Obesity and related comorbidities have become serious health threats all over the world. Bariatric surgery has been shown to be an effective method for severe obesity in both the short and long term. Patients experience significant and sustained weight loss after bariatric surgery. Moreover, the preexist obesity associated comorbidities have been improved or resolved in most patients. A series of clinical researches have demonstrated the safety and validity of bariatric surgery. The clinical applications of surgery therapy provide an alternative for obesity and related comorbidities.

Objective To evaluate the values of contrast-enhanced ultrasound in diagnosis of cystic renal cell carcino-ma. Methods A total of 73 patients with renal cystic lesions were included in this study. The image features of ultrasound and contrast-enhanced ultrasound examination were analysed. All of patients underwent surgical treatment and had patholog-ical results. The diagnostic values of the ultrasound and contrast-enhanced ultrasound were analyzed by evaluating the im-age features of cystic renal cell carcinoma. Results There were 64 cases of cystic renal cell carcinoma, 9 cases of benign cyst. With ultrasound and color doppler ultrasound,irregular shape, thickness wall, solid ingredients, divisions and more blood flow signals were found in cystic renal cell carcinoma. Renal cyst showed regular shape, few solid component and thin separation and inconspicuous blood flow signals. In contrast-enhanced ultrasound, cystic renal cancer contrast agent appear-ing time was (15.13±4.21)s, and reached the peak time (23.42±5.68)s, fade time was (28.42±4.27)s. The enhanced mode for fast in and fast out was found in 22 cases (34.3%), fast in and slow out in 30 cases (46.8%), slow in and fast out in 2 cases (3.2%), slow in and slow out in 4 cases (6.4%), and synchronously in and out in 6 cases (9.3%). The hyper-enhancement was found in 42 cases (65.6%), the iso-enhancement and hypo-enhancement in 22 cases (34.4%). In renal cyst, There were three cases out of contrast filling. In other 6 cases, the contrast agent appearing time was (16.67±2.73)s, the peak time was (25.83±3.06)s and fade time was (34.17±4.26)s. The enhanced mode for fast in and fast out was found in 1 case (16.7%), fast in and slow out in 1 case (16.7%) and synchronously in and out in 4 cases (66.6%). The hyper-enhancement was found in 2 cases (33.3%), the iso-enhancement and hypo-enhancement in 4 cases (66.7%). The sensitivity, specificity, positive predic-tive value, negative predictive value and accuracy of ultrasound were 85.9%, 66.7%, 94.8%, 40.0%and 83.6%. The sensitiv-ity, specificity, positive predictive value, negative predictive value and accuracy of contrast-enhanced ultrasound were 92.2%, 77.8%, 96.7%, 58.3%and 90.4%. Conclusion Contrast-enhanced ultrasound can be used in benign and malignan-cy identification of renal cystic lesion.

OBJECTIVE:To explore the characteristics and regularities of cardiac damage induced by metranidazole,and pro-vide reference for clinical safe application. METHODS:In retrospective study,58 cases of cardiac damage caused by metranidazole in Henan ADR Monitoring Center for Drug from Jan. 2010 to Dec. 2015 were analyzed statistically. RESULTS:In 58 cases of cardiac damage induced by metranidazole,25 cases were male,33 were female,the ratio of male to female was 0.76∶1,the patients with ADR/ADE aged 15-64 years (74.14%),most were intravenous administration (79.31%) and happened within 30 min (70.69%), cardiac damage mainly manifested as palpitation,severe cases can cause tachycardia and arrhythmia,the ADR improved or cured af-ter withdrawal or symptomatic treatment. CONCLUSIONS:Clinic should strengthen observation of ADR/ADE caused by metranida-zole,especially the monitoring of cardiac toxicity reactions;pharmaceutical manufacturers should improve the drug instructions to in-sure the safety of clinical medication.

Objective:To study the feasibility of microarc oxide treatment on the enhancement of titanium-ceramic bonding strength. Method:Titanium samples in the size of 25 mm?3 mm?0.5 mm were prepared with smooth surface(group 1),rough surface(group 2) and microacrc oxide treated surface(group 3). Nickel-chromium alloy samples in the same size were prepared (group 4). The surface of the samples was observed by scanning electron microscope(SEM) and dispersive spectrometry analysis. Then, the samples were bonded to porcelain. The bonding interface was observed by SEM. The bonding strength of the samples was measured by a three-point bending test according to ISO 9693.Results:Microarc oxide treated surface was rough and porous. The interface of microarc oxide treated surface bonded to porcelain was compact. The bonding strength(MPa) of the samples of group 1,2,3 and 4 to porcelain was 30.79?1.3,36.12?3.03,45.84?3.15 and 48.35?3.06 respectively(group 3 vs group 1 or 2 P0.05). Conclusion:The microarc oxide treatment on titanium can increase the titanium-ceramic bond strength.

Objective To investigate the indoor air pollution caused by construction materials and decoration materials and present scientific data for controlling indoor air pollution caused by construction and decoration materials. Methods 126 samples collected from 44 newly decorated rooms in some districts of Luohe city. The levels of ammonia, benzene and formaldehyde were analyzed according to the national standard. Results The rates of exceed standard of benzene and formaldehyde concentration were 8.7% and 72.2% respectively. Formaldehyde concentration was 3.4 times of the national standard limit averagely. Ammonia and dimethylbenzene were not detected. Conclusion Some decoration materials can pollute the indoor air. The formaldehyde and benzene concentration tend to decrease as time goes on.

Objective:To evaluate the effect of preoxidation on the bonding strength of titanium to porcelain.Method:12 titanium plates(25 mm?3 mm?0.5 mm) were pre-oxidated according to usual preoxidation procedure and 12 without preoxidation were used as the controls.The samples(6 in each group) were then bonded with bonding porcelain,opaque porcelain,dentin porcelain and glaze of Duceratin(Degussa) or Vita titankeramik(Vita) respectively.The bonding strength of Ti/porcelain was evaluated using three-point bending test according to ISO 9693 1990 standard. The interface of Ti/porcelain bonding was observed by SEM.Results:Bonding strength(MPa) of Ti/Duceratin preoxided group and the corresponding control was 41.910?2.778 and 33.097?5.297(P0.05),respectively.SEM observation showed inter-lock and tight bonding of Ti/porcelain in pre-oxidated interface,more cracks and gaps between Ti and porcelain in non-pre-oxidated interface.Conclusion:Pre-oxidation may improve bonding strength of Ti/Duceratin system, while has no significant effect on bonding strength of Ti/Vita titankeramik system.

Objective To understand the situation of medication treatment in out-patients with hypertension.Method 1 210 out-patients with hypertension were investigated by ourselves-made questionnaire on their medication treatment at home.Results In all of the patients,there was only 30.74% of them persisting in taking regular medication.The control rate on blood pressure was 70.96% in patients with regular taking medication and 28.99% in patients with irregular taking medication.The rates of regular taking medication in goverment staff,teachers and retirees were higher.The compliance with medication treatment was increased with the age.There was 79.83% of patients discontinued to take medication after 3-6 months of medication treatment.Conclusion The situation of medication treatment in outpatients is less optimistic.We should strengthen the blood pressure controll in outpatients of the young aged,workers and peasants.