Objective To study the expression of KAI1/CD82 in non-small cell lung carcinomas(NSCLC) and explore the mechanism of invasion and metastasis of tumor cells.Methods The S-P immunohistochemistry was used to detect the expression of KAI1/CD82 in 62 specimens of primary NSCLC and 22 specimens of lymph node metastatic tissues. Twenty specimens from the normal tissues adjacent to the tumor over 5 cm were used as negative controls.Results The expression level of KAI1/CD82 in NSCLC(32.26%) was lower than that in negative control tissues(85.0%) (P

0.05).CONCLUSIO_ NS:The two sets of regimen are similar in their efficacies and toxicity,both with satisfactory tolerance.

【Objective】To optimize the functional monomers which is used for the synthesis of Corilagin molecular imprinting polymer.【Methods】The chromatographic characteristics of Corilagin molecular imprinting polymers synthesized respectively by functional monomers of ?-methacrylic acid(MAA)and acrylamied(AA)were investigated with HPLC,and the imprinting efficiency of the two kinds of polymers on Colilagin template were compared.【Results】The polymer with AA as the functional monomer had a higher imprinting efficiency than the polymer with MAA as the functional monomer.【Conclusion】AA is the optimal functional monomer for the synthesis of Corilagin molecular imprinting polymer.

0.975). The deviation of medical decision level was within 1.6. Conclusion Comparison among various analyzers were improved after the other analyzers corrected by the one comparable analyzer through the result of the fresh serums determined by it.

Objective To assess the diagnostic value of the single-photon-emission computed tomography (SPECT) and the magnetic resonance imaging (MRI) for Alzheimer's disease (AD) and vascular dementia (VD), and to explore the correlation between cerebral atrophy and cerebral regional blood flow and the degree of cognitive impairment in AD patients. Methods The temporal and frontal lobes and hippocampus were quantitatively detected by MRI and the cerebral regional blood flow in these regions was analysed semi-quantitatively by SPECT for 36 patients with AD, 32 with VD and 30 sex-matched non-demented elderly controls (NC). Results The hippocampal atrophy of AD and VD groups detected on MRI was significantly different from that of the NC group. Left hippocampal height was (9.27?3.43)mm in AD, (11.62?5.72)mm in VD and (12.13?2.97)mm in NC(P0.05, compared to that of NC), showing that hippocampal atrophy could not be detected by MRI. However, RAR of the left hippocampus by SPECT detection was 0.61?0.07 (P

Objective To evaluate the effect of methylene blue and visible light by FQ-PCR assay on the inactivation of plasma hepatitis B virus,and to establish a direct and impersonal basis for the surveillance of cural inactivation.Methods Four samples of plasma of HBV-DNA positive were added with methylene blue. Then the plasma was processed with visible light radiation(30 000 Lux) for 0, 5, 10, 15,30 min respectively. Moreover,the concentration of HBV-DNA samples was quantitatively detected by the method of FQ-PCR and compared with untreated samples to evaluate the effect of HBV inactivation and its relationship with the time of radiation.Results The original concentration of HBV-DNA was 5.6?107, 3.2?106, 1.8?106,3.5?105 copies/ml respectively. After being added with methyene blue, the concentration of HBV-DNA was significantly decreased(P

ObjectiveTo explore the inflammatory factors C-reactive protein (CRP),interleukin (IL)-6,tumor necrosis factor (TNF)-α and white blood cell (WBC) count differences between acute myocardial infarction(AMI ) thrombolysis treatment and unthormbolysis treatment,and find out the relevance between the inflammatory factors and the prognosis.MethodsAccording to the condition of accepting AMI thrombolysis treatment,the 229 patients of AMI were divided into the thrombolysis group( 131 cases) and the unthrombolysis group(98 cases).The levels of myocardial troponin I (cTnI),creatine kinase(CK),creatine kinase isoenzyme-MB (CK-MB) were detected at the time of patients sent into the hospital for the immediate,6-hour later and 24-hour later.After 6-month's follow-up,prognosis was compared between two groups.ResultsTwenty-seven cases lost in the thrombolysis group.One case died within 6 months and the mortality was 1.0％(1/104) in the thrombolysis group,and 6 cases died within 6 months and the mortality was 6.1％(6/98 ) in the unthrombolysis group.There was significant difference between two groups (P ＜ 0.05 ).The levels of CK,CK-MB in the thrombolysis group advanced,and compared with that in the unthrombolysis group,there were significant differences (P ＜ 0.05 ).The levels of TNF-α,IL-6 in the thrombolysis group were significantly higher than those in the unthrombolysis group (P＜ 0.05),and CRP and WBC count had no significant differences between two groups (P ＞ 0.05).The repatency rate was 79.4％( 104/131 ) in the thrombolysis group,the levels of TNF- α,IL-6 in repatency patients were higher than those in non-repatency patients.There were significant differences(P ＜ 0.05 ).ConclusionsThe thrombolysis is an effective way to cure AMI.The increase of TNF- α,IL-6 after the thrombolysis is considered to be related to reperfusion injury,and CRP,IL-6,TNF-α and WBC count can forecast the inflammation of myocardial necrosis and take an impotant role in predicting the prognosis of the AMI.The antiinflammatory and antioxidation treatment is significant to improve the prognosis of the AMI.

To increase the conclusion rate of scientific research projects and prevent the phenomena of fine start and poor finish,high input and low output,and heavy application and light management therein,by calculating the conclusion rate of the research projects of all levels expired longitudinally undertaken by Fujian Medical University from 2010 to 2012,this thesis analyzes the root cause for the delay of research projects starting from the three parties which sign the research project contract,namely,the competent authority of science and technology,the regulation department of scientific research,and the research group; meanwhile,it puts forward the countermeasures including perfecting the management system of scientific research,implementing effective secondary Strengthen the team management,management of colleges and universities,and strengthening the building of scientific research management team,etc.,in the hope of providing reference for the increase of the conclusion rate of research projects in institutions of higher learning.

The microRNA (miRNA) is a novel class of small noncoding RNA that regulate gene expression by targeting mRNA.It has been shown to play important roles in a broad range of biological processes,especially in the occurrence,development,invasion and metastasis of malignant tumors.Conventional detection methods,such as Northern blot and realtime PCR have been used to assess endogenous miRNA expression.However,these techniques are invasive and cannot be used repetitively in living animals.Recent remarkable advances of in vivo imaging techniques have provided the capability of noninvasive imaging of miRNA in vivo.The recent progress about the reporter gene,antisense luminescence,nanoparticle and multimodal imaging for visualizing miRNA expression in vivo was reviewed.

Objective To investigate the relationship between gene polymorphism of factor VII R353Q (FVII R353Q) and cerebral palsy for Han Chinese children. Methods Polymerase chain reaction-restricted fragment length polymorphism were used to determine genotype and allele of R353Q gene in 160 children with cerebral palsy and 137 normal children. Results The genotype of FVII R353Q is close to Hardy-Weinberg equilibrium in both CP group and control group (P>0.05). There was not significant difference in the distribution of the allelic frequency and genotype of FVII R353Q between the both groups (P=0.436, P=0.182, respectively). Conclusion The gene polymorphism of FVII R353Q is not significantly related with cerebral palsy for Han Chinese children.