1∶640) was purified by affinity chromatography with a Mab Trap kit.The effects of the purified IgGs on the ion channel and transporter(INa/Ca,INa pump,ICa-L,Itko,Ik1,IK) at concentrations of 10 nmol/L,20 nmol/L and 40 nmol/L were observed by whole cell voltage clamp technique.RESULTS:The purified IgG enhanced INa/Ca,ICa-L and I Na/k pump in a dose-dependent manner and no significant effect on INa,Itko,Ik1 and Ik was found.CONCLUSION:The site specific antibody of Na-Ca exchanger stimulates Na-Ca exchanger current and has cross-reaction with L-type Ca channel and Na pump.

Objective To study the effect of the music therapy on the serotonin and tubulin in frontal cortex,hippocampus and hypothalamus of chronic unpredictable mild stress (CUMS) rats,and to explore the neurobiology of the music therapy on stress disorders.Methods Twenty-four male Sprague-Dawley rats were randomly divided equally to music therapy stress group(n=8),stress group(n=8) and normal control group(n=8).Mild chronic unpredictable stress was used for 21 days in a continuous stimulation pattern to establish the CUMS model.The 5-HT,5-HIAA and tubulin levels in the the frontal cortex,hippocampus and hypothalamus were detected.Results The time staying in the centre square in the open field test,the stress group spent less time than that in the control group (P＜0.01),and no significant difference was observed (P＞0.05).Compared with the stress group,the level of 5-HT and 5-HIAA in the hippocampus and the frontal cortex of the music therapy stress and the control group were significantly higher (P＜0.01),however,no significant difference was observed in the hypothalamus among the three groups.Compared with the stress group,the expression of Tyr-tubulin in the hippocampus of the music therapy stress and the control group significantly decreased (P＜0.O1),in contrary,the expression of Acet-tubulin in the hippocampus of the music therapy stress and the control group significantly increased (P＜0.01).However,no significant difference was observed in the frontal cortex and the hypothalamus among the three groups for either Tyr-tubulin or Acet-tubulin (P＞0.05).Conclusions This study indicates that music therapy can increase the release of the 5-HT in the hippocampus and the frontal cortex,and improve the expression of tubulin dynamics of the hippocampus for rats stressed.

Objective To evaluate the clinical significance of laparoscopic operations in the treatment of ovarian endometriosis cysts. Methods We compared clinical data between 52 cases of laparoscopic operation for ovarian endometriosis cysts (Laparoscopy Group) and 75 cases of open operation for ovarian endometriosis cysts (Open Group) in the same period. Results The operation time was significantly shorter in the Laparoscopy Group (61 4?9 3) than in the Open Group (72 6?7 1) ( t =7 692, P =0 000). The time to first flatus was significantly earlier in the Laparoscopy Group (25 3?4 1 h) than in the Open Group (49 8?6 9 h) ( t =22 935, P =0 000). The postoperative pyrexia rate in the Laparoscopy Group (9/52) was significantly lower than that in the Open Group (31/75) ( ? 2 =8 216, P =0 004). There were no significant differences on the remission rate of dysmenorrhea between the Laparoscopy Group (26/37) and the Open Group (34/51) ( ? 2=0 128,P =0 818) and on the postoperative pregnancy rate between the Laparoscopy Group (5/22) and the Open Group (7/25) ( ? 2 =0 171, P =0 747). Conclusions Laparoscopic operation for ovarian endometriosis cysts is as effective as open operation, being an ideal alternative.

Objective To evaluate the value of laparoscopy in the treatment of intramural myoma.Methods Totally 120 cases of intramural myoma were randomly divided into laparoscopy(n=52)and open groups(n=68).Clinical data,including operation time,intraoperative blood loss,rates of postoperative pyrexia and postoperative complications,length of hospital stay and follow-up,were compared between the two groups.Results Compared to the open group,the laparoscopy group had significantly longer operation time (106.3?54.9)min vs(66.5?7.3)min,t=5.918,P=0.000],less blood loss 10-300 ml(median,50 ml)vs 20-200 ml(median,50 ml),Z=-1.998,P=0.046],lower rate of postoperative pyrexia 7.7%(4/52)vs 30.9%(21/68),?2=9.608,P=0.002],similar rate of postoperative complications 3.8%(2/52)vs 8.3%(3/36),?2=0.000,P=1.000],shorter hospital stay (7.5?1.4)d vs(10.4?1.2)d,t=-12.201,P=0.000],and similar length of follow-up (9.7?2.3)months vs(9.6?1.8)months,t=0.267,P=0.790] and rate of normal menses 94.4%(17/18)vs 90.5%(19/21),?2=0.000,P=1.000].Conclusions Laparoscopy is as effective as open surgery for intramural myoma.While the laparoscopy leads to better postoperative recovery than the open surgery.

Objective To evaluate the improvement of endothelial function by folic acid and B vitamins supplement in patients of coronary artery disease who undergone interventional therapy.Methods Eighty patients of coronary artery disease who undergone interventional therapy were divided into two groups:control group(40 cases,routine treatment)and treatment group(the others,with routine treatment plus orally taken folic acid and B vitamins,folic acid 15mg/d,vitamin B_(12)75μg/d,vitamin B_6 30mg/d,for 6 months).Plasma homocystine (Hcy)was measured by fluorescence polarization immunoassay and flow-mediated dilation(FMD)of the brachial artery was assessed by high resolution ultrasound before and after 6 months.Results After treatment,the levels of Hcy decreased obviously in treatment group[(8.62±3.51)μmol/L]than that in control group[(12.96±4.62)μmol/L](P<0.01).The FMD was increased significantly in treatment group[(4.22±1.46)%vs (8.74±1.51)%,P<0.01].Conclusions Routine treatment,including aspirin and stafins,significantly improves endothehal function in patients with coronary artery disease.Folic acid and B vitamins supplement may exert beneficial effects on patients with coronary artery disease.

Objective To develop a rapid method with high sensitivity and specificity to detect Mycobacterium avium.Methods A polymerase chain reaction(PCR)was developed.Its sensitivity and specificity were verified by sequential dilution of DNA of M.avium and other17Mycobacterium spp.,respectively.Simulation of clinical infection was established by mixing normal skin tissue with M.avium.The treatment of the tissue specimens was optimized in order to improve the sensitivity of the PCR assay.Results A fragment of427bp was amplified by the PCR assay with the strains of M.avium at the sensitivity of1?10 2 cells/mL.The other17Mycobacterium spp.were all negative.The sensitivity of the PCR assay decreased to1?10 4 cells/mL when M.avium was mixed with the homogenized skin tissue.The sensitivity recovered to1?10 2 cell/mL when the skin tissue was diluted to≥1∶4.Conclusion It is suggested that PCR be a rapid and reliable method for detection of M.avium.

Highly active adherent LAK cells (A-LAK) with monocytes depleted by phenylalahine methyl ester (PME) were cultured from peripheral blood lymphocytes of patients with hepatocellular carcinoma (HCC). Results showed that A-LAK cells cultured in about 14 days had expanded better and faster than that of nonadherent LAK cells (NA-LAK) with their greatest expansion varied from 23 to 243 fold .A-LAK cells showed a trend of increase in TH cell subgroup and decrease in Ts cell sub-group as well as significant difference of TH/TS ratio. The IL-2R expression increased from 34.1%to 64.3%. A-LAK cells had a higher cytotoxicity (64.6%)than that of NA-LAK cells (42.8%).Further clinical application of A-LAK cells may improve biotherapeutic effect on HCC patients compared with that of NA-LAK cells .

Objective[WT5”BZ] To evaluate the role of radionuclide 99m Tc EHIDA for hepatobiliary imaging in rats with orthotopic liver transplantation (OLT) [WT5”HZ]Methods[WT5”BZ] Male SD rats were divided into 2 groups: normal controls (NL, n =10)and normal rats with OLT (NL OLT, n =8). Dynamic scintigraphy using single photon emission computed tomography (SPECT) was used for hepatobiliary imaging. [WT5”HZ] Results[WT5”BZ] The time of peak activity (t max ) and half peak activity(t 1/2 ) in the liver and intestine (GI) imaging time (min) in NL and 6 uncomplicated NL OLT rats were 3 05?0 31 vs. 3 63?0 65 ( P 0 05), respectively. The time active curves and imaging of NL OLT rats were similar to that in NL rats.The t max and t 1/2 in the liver, GI imaging time and the time active curves in 2 complicated NL OLT rats were significantly different compared with that of NL rats. Radionuclide leak occurred in 1 NL OLT rat.[WT5”HZ] Conclusions [WT5”BZ] Radionuclide 99m Tc EHIDA effectively monitors both structural and functional changes of the liver graft. [WT5”HZ]

ObjectiveTo explore the inflammatory factors C-reactive protein (CRP),interleukin (IL)-6,tumor necrosis factor (TNF)-α and white blood cell (WBC) count differences between acute myocardial infarction(AMI ) thrombolysis treatment and unthormbolysis treatment,and find out the relevance between the inflammatory factors and the prognosis.MethodsAccording to the condition of accepting AMI thrombolysis treatment,the 229 patients of AMI were divided into the thrombolysis group( 131 cases) and the unthrombolysis group(98 cases).The levels of myocardial troponin I (cTnI),creatine kinase(CK),creatine kinase isoenzyme-MB (CK-MB) were detected at the time of patients sent into the hospital for the immediate,6-hour later and 24-hour later.After 6-month's follow-up,prognosis was compared between two groups.ResultsTwenty-seven cases lost in the thrombolysis group.One case died within 6 months and the mortality was 1.0％(1/104) in the thrombolysis group,and 6 cases died within 6 months and the mortality was 6.1％(6/98 ) in the unthrombolysis group.There was significant difference between two groups (P ＜ 0.05 ).The levels of CK,CK-MB in the thrombolysis group advanced,and compared with that in the unthrombolysis group,there were significant differences (P ＜ 0.05 ).The levels of TNF-α,IL-6 in the thrombolysis group were significantly higher than those in the unthrombolysis group (P＜ 0.05),and CRP and WBC count had no significant differences between two groups (P ＞ 0.05).The repatency rate was 79.4％( 104/131 ) in the thrombolysis group,the levels of TNF- α,IL-6 in repatency patients were higher than those in non-repatency patients.There were significant differences(P ＜ 0.05 ).ConclusionsThe thrombolysis is an effective way to cure AMI.The increase of TNF- α,IL-6 after the thrombolysis is considered to be related to reperfusion injury,and CRP,IL-6,TNF-α and WBC count can forecast the inflammation of myocardial necrosis and take an impotant role in predicting the prognosis of the AMI.The antiinflammatory and antioxidation treatment is significant to improve the prognosis of the AMI.

ObjectiveTo approach effects of acupuncture on ganglioside in rats with acute cerebral ischemia.Methods204 Wistar rats were randomly divided into the normal group, model group, acupuncture group and medicine group, and middle cerebral artery embolism has been made by thread-ligation method in the latter three groups. Ganglioside content in the rat brain had been measured respectively after cerebral ischemia 1,2,3,5,7,12 and 24 hours.ResultsThe content of lipid-bound sialic acid (LBSA) has decreased significantly after the acute cerebral ischemia, but LBSA contents of the acupuncture group and medicine group were higher significantly than that of the model group, and these two groups have been similar in curative effect, and have difference only at embolism 2,3 and 5 hours.Conclusion Acupuncture can regulate ganglioside and play the role of cerebral protection and neuroplasticity.