Oridonin is a deterpenoid isolated from Rabdosia rubescences. It has excellent antitumor effect, whose mechanism is to induce apoptosis of tumor cells by mitochondrial pathway. Signal transduction pathway play an important part in cell proliferation,differentiation,apoptosis and so on. Recent studies suggested that Oridonin may trigger mitochondrial apoptosis pathway by four signal transduction pathways,which were p53, Ras/Raf/ERK, PI3K/Akt and NF-κB pathway. Further investigation on mitochondrial signal transduction pathway would be helpful to clarify the intracellular target and the antitumor molecular mechanism of ORI.

Objective To evaluate the improvement of endothelial function by folic acid and B vitamins supplement in patients of coronary artery disease who undergone interventional therapy.Methods Eighty patients of coronary artery disease who undergone interventional therapy were divided into two groups:control group(40 cases,routine treatment)and treatment group(the others,with routine treatment plus orally taken folic acid and B vitamins,folic acid 15mg/d,vitamin B_(12)75μg/d,vitamin B_6 30mg/d,for 6 months).Plasma homocystine (Hcy)was measured by fluorescence polarization immunoassay and flow-mediated dilation(FMD)of the brachial artery was assessed by high resolution ultrasound before and after 6 months.Results After treatment,the levels of Hcy decreased obviously in treatment group[(8.62±3.51)μmol/L]than that in control group[(12.96±4.62)μmol/L](P<0.01).The FMD was increased significantly in treatment group[(4.22±1.46)%vs (8.74±1.51)%,P<0.01].Conclusions Routine treatment,including aspirin and stafins,significantly improves endothehal function in patients with coronary artery disease.Folic acid and B vitamins supplement may exert beneficial effects on patients with coronary artery disease.

Schwann cells are the seed cells of tissue engineedng in the regeneration of peripheral nerve.so whether we can harvest enough Schwann cells of high purity is very important in the tissue engineering.Culture and purification technology of Schwann cells has been improved day by day.The classical cultural methods include tissue-clump cultural method and enzyme digestion cultural method.On the basis of the classical cultural methods,someone uses new technologies to delete fibroblasts,such as addition of cytosine arabinoside,addition of antimitogenic agents.The latest purified methods include magnetic activated-cells separation,co-culture with three-dimensional scaffolds and laminin-coated wells purified method.The aim of the skills above is to provide enough Schwann cells for the tissue engineering repeir of degenerated peripheral nerves.There are many ways of Schwann cells culture and purification.and we have made a primary progress,but it still needs further studies before the entire achievemenL such as long cycle,low activity in cultured calls,and the instable biological characteristics during passage culture,etc.

Objective To investigate the cognition level of clinical nurses to peripheral venous indwelling needles, and provide a reference for nursing administrators to carry out targeted intravenous catheter standardized training operation. Methods By convenience sampling, 129 clinical nurses of the certain grade A class three hospital were selected, and their perception about indwelling intravenous catheter were investigated by using self-designed questionnaire in November 2015. Results 125 questionnaires were effectively rewnered. Average score of 125 nurses′cognition to indwelling intravenous catheter was (57.52 ± 10.23) points, pass rate was 65.6%(82/125), and influencing factors were education background, the job tittle, work experience and so on. Conclusions The clinical nurses′assessment, cognition and knowledge master to indwelling intravenous catheter is not ideal, lacking a comprehensive clinical peripheral venous catheter assessment tools. Clinical nurse managers should focus on strengthening vein management, reasonable and better trained clinical nurse intravenous catheter cognitive level, so as to ensure that the effect of catheter using.

BACKGROUND: In recent years, increasing studies focus on digital complete denture. Digital complete denture has many advantages such as reducing patient visit times, saving chairside time, improving manufacturing precision in comparison with traditional complete denture.OBJECTIVE: To review the development of technology and clinical application of digital complete denture,and to analyze the existing insufficiencies.words were CAD/CAM complete dentures, digital complete dentures, rapid prototyping dentures, manufactured dentures, computer dentures, machined dentures, designed dentures, milled dentures, artificial tooth, 3D scanning, 3D printing in English and Chinese, respectively.RESULTS AND CONCLUSION: Currently, the production of complete denture using digital technology cannot be fully realized. During digital impression, to scan the traditional dental casts is still the mainstream technology for data acquisition technology. Future investigations on scanning the patient alveolar ridge and its surrounding soft tissue directly and digitally recording the jaw relation directly are warranted. To develop 3D-printed artificial teeth and base materials with proper strength, aesthetics, comfort levels as well as 3D printing of the artificial tooth and the base as the final denture will make digital denture technology more mature.

Objective To explore the effect of early intervention on myelin-associated glycoprotein (MAG) expression of white matterin neonatal rats with cerebral brain injury caused by intrauterine infection. Methods 17 days or 18 days pregnant Wistar rats were consecutivelyinjected with lipopolysaccharide (LPS) intraperitoneally. 100 neonatal rats born from LPS group were randomly divided into interventiongroup and non-intervention group with 50 in each group. Another 10 pregnant rats were injected with normal saline, 50 neonatal rats born from the the normal saline group were taken as control group. The intervention group received early intervention and rehabilitationtraining. All the groups underwent hanging test and modified BBB test on the 14th, 21st and 28th day. 6 rats in each group were tested by immunohistochemicalstaining to observe the MAG expression in white matter on the 1st, 7th, 14th, 21st, 28th day. Results The scores of hangingtest and modified BBB test were significantly higher in the control group than in the LPS group (P<0.01), and higher in the interventiongroup than in the non-intervention group (P<0.01). The expression of MAG was higher in LPS group than in the control group (P<0.01), innon-intervention group than in the intervention group (P<0.01). Conclusion Early intervention and rehabilitation training can improve themotor function in rats with brain injury and reduce the expression of MAG.

Objective To reveal the present situation of geriatric nursing, summarize the important research topics, in order to determine the focus of the research and work of geriatric nursing in China. Methods To take geriatric nursing belonged to MeSH thesaurus as key words, related literatures from 2006 to 2016 in Web of Science core collection were searched, then clustering analysis were adopted to analyze highly cited papers. Results Totally 2148 pieces of literatures related to geriatric nursing, there were 80079 pieces of quotations. The contents involved inappropriate medication use and falls in geriatric nursing risk management, functional decline, delirium and chronic cognitive impairment of the elderly, distinguishing between the basic theory of geriatric diseases, tools and practice of geriatric nursing assessment. Conclusions The current research hotspots on geriatric nursing includes: the risk management of geriatric nursing, prevention and control of geriatric syndrome, systematic study on geriatric nursing assessment.

Objective To investigate the association between apolipoprotein E (apoE) genotype polymorphism and cerebral palsy (CP) in Han Chinese children. Methods Two hundred and forty Han Chinese children were selected, including 120 children with cerebral palsy (CP group) and 120 normal children (control group).Polymerase chain reaction-restricted fragment length polymorphism analysis was used to determine the genotype and alleles of apoE genotype in the two groups. Results The genotype distributions of apoE in the two groups were consistent with the existence of a Hardy-Weinberg equilibrium. There were significant differences in the distributions of allelic frequency and apoE genotype between the two groups. Carrying the ε4 allele was related to cerebral palsy, and the overall risk for cerebral palsy was elevated about 5.5-fold among children carrying the ε4 allele.Conclusions There is an association between apoE genotype and cerebral palsy, and apoE's ε4 allele is the associated risk factor.

Vasculogenic mimicry (VM) can promote tumor growth, invasion, and metastasis. The formation of VM is regulated by various proteins including epithelial cell kinase, phosphoinositide-3 kinase, and hypoxia-inducible factor 1. Local microenvironment also plays an important role in regulating VM formation.

BACKGROUND: Collagen sponge is considered the most useful biomaterial owing to its excellent function and properties, easy processing, sterilization, and preservation and has been widely used in scientific research and clinical application. OBJECTIVE: To describe the properties of collagen sponge and to review the research progress of collagen sponge in clinical application in recent years. METHODS: A computer-based retrieval was performed by the first author to search for manuscripts published from January 2000 to August 2010 in Pubmeds and Elsevier databases and for manuscripts published from January 1993 to August 2010 in CNKI database using key words "collagen, collagen sponge, clinical application" in title and abstract items in English and Chinese language, respectively. RESULTS AND CONCLUSION: Collagen sponge, as a new biological material, has been widely applied in tissue engineering research in terms of hemostasis, wound healing, anti-infection, cartilage repair, and nerve repair. But at present, nearly all collagen is from animals and immunogenicity cannot be thoroughly eliminated. Researchers outside of China have synthesized recombinant human collagen using bioreactor and transgenic technology, but its efficacy and safety in clinical application needs further investigation and research.