1.Effects of cyclin dependent kinase 5 inhibitor Roscovitine on remifentanil-induced hyperalgesia
Xiaojie LIU ; Xiaoping GU ; Wei ZHANG ; Jing ZHANG ; Zhengliang MA
Chinese Journal of Behavioral Medicine and Brain Science 2011;20(11):961-963
ObjectiveTo study the effects of intrathecal injection of cyclin dependent kinase 5 inhibitor Roscovitine on the hyperalgesia induced by remifentanil in a rat model of incisional pain.MethodsForty-five SD rats were randomly divided into 5 groups ( n =9 in each group):control group ( C ),incisional pain group ( Ⅰ ),Roscovitine group(ROS),remifentanil group(R) and Roscovitine + remifentanil group ( ROS + R).Roscovitine (50μg/10μl) was injected intrathecally at 30 min before plantar incision in groups ROS and ROS + R,other groups were injected with 20% DMSO(10μl).All groups except for C group needed to be made the model of incisional pain.In group R and ROS + R,remifentanil(0.04 mg/kg) was infused subcutaneously with a pump for 30min at the moment of surgical incision.The paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency(PWTL) were used to evaluate the behavioral changes and measured at 24 h before incision and at 2 h,6 h,24 h and 48 h after incision.ResultsCompared with group C,the PWMT and PWTL of group Ⅰ were significantly decreased after incision (P<0.01).Compared with group Ⅰ,the PWMT and PWTL of group R were significantly decreased after incision (P < 0.01 ) ; however,the PWTL was significantly increased (2h:( 20.26 ± 1.33)s,(17.97 ±0.47)s;48h:(22.15 ±0.660)s,(19.89 ±1.27) s),P<0.05)in ROS group.Compared with group R,at 2h after incision,the significant increase of PWTL ( ( 19.13 ± 1.72)s,( 14.41 ± 2.30) s) and PWMT ( ( 10.4 ± 1.95 ) g,(6.38 ± 0.91 ) g) were observed,then lasted up to 48 h ( ( 19.24 ± 2.8 ) s,( 14.87 ± 1.95 ) s )and 24h ( (8.88 ± 1.41 ) g,( 6.83 ± 0.80) g) respectively in group ROS + R (P < 0.05 ).ConclusionIn a rat model of postoperative pain,Roseovitine could reduce the thermal hyperalgesia purely induced by incision,and also could prevent the development of hyperalgesia induced by remifentanil.
2.Mycoplasma pneumoniae-associated mucositis: a case report.
Jing YIN ; Xiaojie LI ; Li LIU ; Jian HU ; Chongwei LI
Chinese Journal of Pediatrics 2014;52(5):399-400
Anti-Bacterial Agents
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administration & dosage
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therapeutic use
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Anti-Inflammatory Agents
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therapeutic use
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Azithromycin
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administration & dosage
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therapeutic use
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Biomarkers
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blood
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Child
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Eyelids
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pathology
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Humans
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Immunoglobulin M
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blood
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Lip
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pathology
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Male
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Methylprednisolone
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administration & dosage
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therapeutic use
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Mucositis
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diagnosis
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drug therapy
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microbiology
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Mycoplasma pneumoniae
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drug effects
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isolation & purification
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Pneumonia, Mycoplasma
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complications
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diagnosis
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drug therapy
3.The diagnosis value of TI-RADS and sonographic characteristics for papillary thyroid carcinoma and follicular thyroid carcinoma
Jing ZHAO ; Lihui ZHAO ; Xiaojie XIN ; Hailing WANG ; Sheng ZHANG
Chinese Journal of General Surgery 2016;31(9):754-757
Objective To investigate the value of TI-RADS for sonographic diagnosis of papillary thyroid carcinoma (PTC) and follicular thyroid carcinoma (FTC) and to evaluate the sonographic findings of FTC compared to PTC.Methods The clinical and sonographic data of 363 cases of pathology confirmed thyroid nodules were retrospectively analyzed.The thyroid nodules were classified by TI-RADS grading criteria,the accuracy of TI-RADS and sonographic findings between FTC and PTC was analyzed.Results In 363 thyroid nodules,114 cases were confirmed as benign lesions;249 cases were confirmed as carcinomas,including 50 FTC cases and 199 PTC cases.The sensitivity,specificity,PPV,NPV,and accuracy of PTC were 97.0% (193/199),85.1% (97/114),91.9% (193/210),85.8% (97/113),95.7% (290/303) respectively.The sensitivity,specificity,PPV,NPV,and accuracy of FTC were 97.0%(193/199),85.1% (97/114),91.9% (193/210),85.8% (97/113),95.7% (290/303) respectively.There were significant differences of nodule size,shape,margin,echogenecity,calcification,halo and flow between FTC group and PTC group (P < 0.05).Conclusion TI-RADS has higher value in the sonographic diagnosis of PTC than that of FTC.FTC are greatly different from PTC in many sonographic findings making it less valuable for the diagnosis of FTC.
4.Prospect of Ultra Performance Convergence Chromatography Technology and Applications in TCM Research and Quality Control
Yongwei XU ; Jing HUANG ; Qinglong SUN ; Xiaojie TAN ; Yu KATE
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(3):543-548
The research and quality control of traditional Chinese medicine has meaningful importance, because it has influence not only in the health and treatment of patients, but also in the solid growth and development of phar-maceuticals companies. In some cases, for the complex of TCM, the common QC method on single or multi-target compounds can't really and truly disclose the quality of the Chinese materia medica. Therefore, a lot of researchers do plenty of works to make clear the effectiveness basis, to improve the quality and realize the modernization of TCM. All of these works close together with modern analysis and separation technology. In this article, a novel analy-sis technology-UltraPerformance Convergence Chromatography (UPC2) based its characters and applications should be introduced. It should be a helpful technology for the TCM researchers to facilitate the study and QC works.
5.The Survey on the Occupational Exposure of Undergraduate Nursing Students In Kunming Medical University
Zhengying XU ; Dongmei LI ; Yuan TIAN ; Xiaojie GONG ; Jing JIN
Journal of Kunming Medical University 2013;(10):155-158
Objective To understand the undergraduate nursing students’ awareness of the basic situation of occupational exposure in Kunming Medical University, and to understand their knowing status of occupational exposure knowledge. Methods We used self- designed questionnaire to investigate 449 undergraduate nursing students in Kunming Medical University to obtain the status of occupational exposure and protection knowledge level of students.Results (1) 449 students were investigated, and 258 of them had been in clinical practice, and occupational exposure occurred in 48.8% of them. (2) Occupational exposure factors included grade, washing hands and wearing gloves;The results showed that:Grade was a risk factor and wearing glove and washing hands were protective factors. (3) There was relevance between accepting the occupational protection education and the occupational exposure. Conclusions A useful measure to reduce the occurrence of occupational exposure is Pre-service training for undergraduates nursing students. Operation should be in strict accordance with operating specifications. Schools and departments should implement occupational protection system, so as to strengthen the related knowledge vocational education,enhance self-protection awarenes,and reduce or minimize the occurrence of occupational exposure.
6.Risk Factors of Hospital Infection among Inpatients
Xiaojie JING ; Mingqing CHEN ; Zhan YANG ; Wenli WEI ; Yongkun HUANG
Chinese Journal of Nosocomiology 2004;0(10):-
OBJECTIVE To investigate the risk factors and to take some useful measures to prevent and reduce infection in order to enhance medical quality,to ensure medical security,to strengthen hospital infection manangement and to prevent hospital infection effectively. METHODS We investigated the prevalence rate of hospital infection among our hospitalized patients in 2001,2003 and 2005, respectively. RESULTS The hospital infection rate was 4.6-6.42% in these years.Risk factors and the abuse of antibiotic were decreasing. CONCLUSIONS In order to control hospital infection rate,mensures should be taken including intensively monitoring the departments with high infection rate,strengthening hospital operation,rationally using the antibiotics,and studying the management for hospital infection.
7.Study of quantity of neuron and ultrastructure in nigra-striatum of cerebral palsied newborn rabbits
Xiaojie LI ; Ying YANG ; Zhimei JIANG ; Lin LI ; Jing GAO
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(07):-
Objective To explore the quantity and ultrastructure of dopaminum (DA) neuron in nigra-striatum, pathological basis of the successful animal model of cerebral palsy (CP) by hyperbilirubinemia, to provide the evidence for clinical therapy and rehabilitation. Methods Thirty homozygous newborn rabbits (2~5 d) were randomly divided into 2 groups: a control group(n=10) and a model group(n=20). The rabbits in the control group were administrated bilirubin intraperitoneally, with a total dose of 300 mg/kg, while those in the model group were administrated with saline at the same dose. All the animals were fed well for 45 days until they were sacrificed for observation of the ultrastructure of brain tissue and quantification of DA neurons. Results The ultrastructure of nigra-striatum of the control group demonstrated significant changes; The quantity of nigra-striatum DA neurons of the control group decreased. Conclusion The ultrastructure of nigra-striatum of the control group has had obviously changes. The nigra-striatum DA neuron of CP rabbits was impaired and the quantity of nigra-striatum DA neuron of CP rabbits was reduced.
8.Effects of Electroacupuncture on Learning and Memory and Expression of Brain-derived Neurotrophic Factor and p75 Neurotrophin Receptor in Rats with Cerebral Ischemia-reperfusion
Xiaojie LI ; Ruhui LIN ; Jing TAO ; Congkuai ZHAO ; Kunqiang YU ; Chunyan LI ; Hongwei PENG ; Lidian CHEN
Chinese Journal of Rehabilitation Theory and Practice 2015;21(9):1020-1024
Objective To observe the effects of electroacupuncture on learning and memory, and discuss the therapeutic mechanism. Methods 45 Sprague-Dawley rats were randomly divided into sham group (n=15), model group (n=15) and electroacupuncture group (n= 15). The latter 2 groups were modeled with middle cerebral artery occlusion for 1.5 h and reperfusion. The rats of electroacupuncture group received electroacupuncture at Shenting (DU24) and Baihui (DU20) for 7 days. Learning and memory ability was tested with Morris water maze. Neurologic impairment was assessed with Longa's score. Their hippocampus were observed under HE staining and the level of brain-derived neurotrophic factor (BDNF) and p75 neurotrophin receptor (p75NTR) protein were determined with Western blotting. Results Compared with the model group, the latency of water maze decreased and the times crossing the platform increased in electroacupuncture group (P<0.05), while the Longa's score significantly reduced (P<0.05), and the lesion of nerve cells were alleviated, with the decrease of p75NTR and increase of BDNF in the ischemic hippocampus (P<0.01). Conclusion Electroacupuncture can improve the learning and memory of cerebral ischemia-reperfusion rats, which may relate with up-regulating BDNF and down-regulating p75NTR in hippocampus.
9.Provision capacity research of primary healthcare services in rural China
Zhiyuan HOU ; Qingyue MENG ; Xiaojie SUN ; Jing YUAN ; Shuang ZHONG ; Miaomiao ZHAO
Chinese Journal of Hospital Administration 2009;25(11):745-748
Objective Studying the status quo and constraints for rural healthcare service in grassroot rural healthcare units,for policy recommendations.Methods Using data from the fourth healthcare service investigation,by means of quantitative interview and qualitative interview,for an investigative interview of 348 township hospitals and 251 village clinics in 31 provinces in China.Results Deployment percentage of primary heahhcare services at township hospitals level is 49.1% (28.0/57),and that for village clinics is 60.6%(5.4/9).Conclusions The key to upgrading rural healthcare service system at grassroots level is to deploy better diagnostic equipments,upgrade the diagnostic competence of grassroots healthcare personnel and build a continuous service system for primary healthcare service.
10.The Status research of ICU Nurses career situations of tertiary general hospital in Shangdong Province
Hong LIU ; Xuebing JING ; Xiaojie FANG ; Tingting GAO ; Cuiping LI ; Xiaoting FAN
Chinese Journal of Practical Nursing 2016;32(25):1978-1981
Objective To understand the status quo of ICU nurses career situations and its influencing factors in Shandong province. Methods A total of 247 ICU nurses were investigated by questionnaires on career condition. Results The career condition score was 79.19 ± 11.63. In addition, statistical significances were revealed by occupational career questionnaire of ICU nurses in different aspect of the scores with age, gender, working years in the ICU, access years to ICU nurses specialist, whether to participate in career training. The scores of career condition and career identity ICU nurses were different, the 40-years-older group was higher than 31-40 years group (P=0.007, P=0.005). The dimension scores of male nurses organizational commitment was higher than that of female nurses(P=0.002). The scores of career condition whose ICU working life more than 16 years were higher than those of within 5 years(P=0.013). The career condition scores of the ICU nurses with access years of specialist qualifications 7-9 years was higher than those of within 3 years(P=0.011). Conclusions The career condition score of ICU clinical nurse specialists was at a medium level, it still needs to be further improved, managers should pay attention to the different levels of nurses to provide career training, in order to promote the professional development of critical care nurses.