Objective: The effect of oil of Hippophae rhamnoides (OHR) supplements on sGOT,metabolism of free radical and ultrastructure of myocardium was observed in mice.Methods: 70 male ICR mice were randomly divided into six groups: sedentary control group (SCG), sedentary+OHR group (SHG), immediately after exhaustive exercise in control group (IEC) and in OHR group (IEO), 24 hours recovery after exhaustive exercise in control group (REC) and in OHR group (REO). After 6 w swimming program with gradually increasing intensity, these mice carried out an exhaustive swimming before sacrifice. Serum GOT, SOD and MDA in myocardium were measured and the changes of ultrastructure of myocardium were observed by transmission electron microscope (TEM). Results: 1) Serum GOT was significantly different among these groups except between SCG and SHG, IEC and REC. 2) SOD was significantly different among the above groups except between SCG and SHG, IEO and REO. 3) The concentration of MDA was significantly different among the above groups except between IEC and IEO, REC and REO. 4) The integrity of mitochondrion, rough surfaced endoplasmic reticulum and myofilament in myocardium was better in OHR groups than in control groups and the pathological tendency of these ultrastructures was lighter in OHR groups compared with control groups. Conclusion: OHR supplementation can reduce the damage of myocardium caused by exercise and enhance antioxidative status of myocardium; OHR supplementation can protect the ultrastructure of myocardium.

catechin plus dexamethasone-treated group. Compared with nephrotic group, the renal pathologic score were significantly different among the nephrotic group and the catechin-treated group (6 80?0 84,P

Objective To observe the clinical efficacy of transcutaneous electric acupoint stimulation in intervening early-stage swelling limb after breast cancer operation.Method A hundred patients undergone radical mastectomy for breast cancer were divided into a treatment group and a control group, 50 cases in each group. The control group was intervened by ordinary post-operative nursing, while the treatment group was additionally given transcutaneous electric acupoint stimulation. By measuring the circumference of both upper arms, the incidence rate and degree of swelling arm in 2 weeks after operation were compared, meanwhile, the accompanied symptoms such as tightness of skin, pain in upper limb, numbness in upper limb, and heaviness of upper limb were observed.Result The occurrence rate of abnormal upper-limb sensations was 22.0% in the treatment group versus 84.0% in the control group, and the difference was statistically significant (P<0.01). The occurrence rate of upper-limb swelling was 4.0% in the treatment group, versus 24.0% in the control group, and the difference was statistically significant (P<0.01). Conclusion Transcutaneous electric acupoint stimulation can effectively prevent and release the early-stage upper-limb swelling after breast cancer surgery.

Objective To evaluate renal vascular damage (RVLs) and detect the expression of miR-145 in children with lupus nephritis (LN).Methods Clinical data of 41 cases of LN diagnosed by renal biopsy from the children with systemic lupus erythematosus (SLE) were collected. Glomerular damage score and RVLs were evaluated. The children were divided into groups according to RVLs score and pathological pattern. In situ hybridization was performed to detect the expression of miR-145 in kidney blood vessel. Differences in RVLs, miR-145 expression in the renal blood vessels and glomerular damage score were observed among the groups with different renal pathological pattern. Differences in clinical parameters, glomerular damage score and miR-145 expression in the renal blood vessels were investigated among groups with different RVLs. Results Among the groups with different pathological pattern, there was no difference in RVLs (P>?0.05) while signiifcant different were found in the expression of miR-145 and glomerular damage score (P??0.05) while a statistical different was found in the expression of miR-145 (P?

Objective To research Henoch-Schonlein purpura purpura (HSP) and renal pathology in children.Methods 31 hospitalized HSP children that with normal urine routine and accepted renal biopsy in our hospital.Results There were different levels of kidney pathological damage in this group of 31 cases,the results of light microscope were from grade Ⅱ to grade Ⅵ The proportion was grade Ⅱ(35.48％,11 of 31),grade Ⅲ (54.83％,17 of 31),and grade Ⅳ,Ⅴ and Ⅵ (each 1 case of 31,3.23％ ).lmmunofluorescence pathology results were showed as following:merely IgA depositional (48.38％,15 of 31 ),IgA + IgG depositional ( 19.36％,6 of 31 ),IgA + IgM depositional ( 19.36％,6of 31 ),IgA + igG + IgM depositional ( 12.90％,4 of 31 ).Microalbuminuria had been founded in 14 cases,and the microalbuminuria level of 10 cases were higher than normal value( 10 of 14,71.43％ ).Conclusions HSP children had renal pathologic dysfunction,even the urine routine were normal,and the detection of urine microalbumin was a significant marker in the early stage.

A homozygous A to G transition (AGT to GGT) in codon 16 of growth hormone receptor (GHR) gene was found in one patient with idiopathic short stature(ISS), resulting in an amino acid change(Ser16Gly). This may be a novel GHR gene mutation; and another novel Arg43Gln GHR gene polymorphism was found in Chinese people.

AIM: To elucidate the significance of glucocorticoid (GC) receptor isoform ?(GR?)in children with idiopathic nephrotic syndrome (INS) , and to evaluate the effect of sera from GC-resistant INS on the expression of GR?. METHODS: The percentage of GR? positive staining peripheral blood mononuclear cells (PBMC) and the quantity of nuclear protein of GR? in PBMC were detected by immunocytochemistry and Western blotting assay, respectively. The effect of sera isolated from children with GC-resistant INS on GR? expression was examined by cell culture in vitro . RESULTS: The number of GR? positive staining PBMC and the quantity of nuclear protein of GR? in children with GC-resistant INS were significantly higher than those in patients with GC-sensitive INS ( P

Objective:To analyze the podocalyxin (PCX) expression in the kidney and the number of urinary podocytes in different pathological grades of Henoch-Sch(o)nlein purpura nephritis (HSPN),and to determine whether the number of urinary podocytes reflects the renal damage in HSPN.Methods:Fifty-six children diagnosed with HSPN in our hospital were enrolled in the study and classified into 4 groups by renal pathology:grade Ⅱ (Ⅱa+Ⅱb) (n=10),grade Ⅲ (Ⅲa+Ⅲb) (n=21),grade Ⅳ (n=16),and grade Ⅴ (n=9).Four kidney autopsy specimens without histomorphologic lesions and 8 urine samples from healthy children served as controls.With immunofluorescence assay,the PCX expression in 4 normal renal tissues and in the renal tissues of the 56 HSPN children was detected and quantitatively analyzed.Positive rate and the number of urinary podocytes were detected in the 8 healthy children and 56 HSPN children.Results:In the renal tissues of the normal control group and grade Ⅱ (Ⅱa+Ⅱb) HSPN group,the PCX expression was complete.The percentage of the PCX positive area out of the total glomerular area in the renal tissues of 2 groups had no significant difference (P＞0.05).In the renal tissues of grade Ⅲ (Ⅲa+Ⅲb),Ⅳ,and Ⅴ HSPN groups,the PCX expression showed various degrees of loss,decreasing in turn from grade Ⅱ (Ⅱa+Ⅱb),Ⅲ (Ⅲa+Ⅲb),Ⅳ to Ⅴ,with significant differences between each group (P＜0.01).For HSPN with grade Ⅲ (Ⅲa+Ⅲb) or higher,positive PCX expression was found in the urine,suggesting the presence of enough podocytes in the urine.The percentage of fluorescence positive area out of the total glomerular area of PCX in the renal tissues was negatively correlated with the total number of urinary podocytes (r=-0.637,P＜0.01).Conclusion:Podocyte injury plays a certain role in the pathological progression of HSPN.The urinary detection ofpodocytes can reflect the degrees of pathological damage in HSPN.

Objective To investigate the changes in the concentration of myocardin in children with lupus nephritis (LN) under different degree of vessel damage.Methods Forty-nine children diagnosed with LN by routine tissue immunolfuorescence, light microscope, and electron microscope were included, and 30 healthy children were included as control group. The pathological classiifcations were performed according to the ISN/RPS 2003 LN pathological classiifcation criterion. According to the Katafuchi evaluation method, the semi quantitative assessment of glomerular and kidney tubule damage was carried out, and the degree of vascular damage was evaluated at the same time. Double antibody sandwich method was used to detect the concentration of serum myocardin.Results The glomerular and kidney tubules damage in children with LN were signiifcantly aggravated with higher pathological classification (P<0.05). Glomerular damage was positively correlated with renal interstitial damage (r=0.96, P<0.01). The degree of vascular damage was related to the degree of glomerular injury and renal interstitial injury, while it was no related with the results of clinical tests. There were different concentrations of myocardin among mild-, moderate-, severe-vessel damage and control groups (F=378.61,P<0.001), and the concentration of myocardin in moderate- and severe-vessel damage groups were obviously lower than those in control group and mild-vessel damage group (P<0.01) while there was no difference between control group and mild-vessel damage group (P>0.05). According to pathological type, there were signiifcant differences in the concentration of myocardial between control group and different pathological types (F=626.793,P<0.01). FromⅡ,Ⅲ,Ⅲ+Ⅴ,Ⅳ toⅣ+Ⅴ, the concentrations of myocardial were decreased systematically, and there were statistic differences between groups (P all<0.05).Conclusion The concentration of myocardin in children with LN can relfect the renal vascular damage to a certain extent. Elevation of myocardin concentration may be helpful for the repair of vascular damage.

To investigate the effects of catechin on angiotensin-converting enzyme (ACE) activity, angiotensin II(Ang II) content and microvessel density (MVD) in renal tissues of 5/6 nephrectomized rats.