Objective To study the effect of the music therapy on the serotonin and tubulin in frontal cortex,hippocampus and hypothalamus of chronic unpredictable mild stress (CUMS) rats,and to explore the neurobiology of the music therapy on stress disorders.Methods Twenty-four male Sprague-Dawley rats were randomly divided equally to music therapy stress group(n=8),stress group(n=8) and normal control group(n=8).Mild chronic unpredictable stress was used for 21 days in a continuous stimulation pattern to establish the CUMS model.The 5-HT,5-HIAA and tubulin levels in the the frontal cortex,hippocampus and hypothalamus were detected.Results The time staying in the centre square in the open field test,the stress group spent less time than that in the control group (P＜0.01),and no significant difference was observed (P＞0.05).Compared with the stress group,the level of 5-HT and 5-HIAA in the hippocampus and the frontal cortex of the music therapy stress and the control group were significantly higher (P＜0.01),however,no significant difference was observed in the hypothalamus among the three groups.Compared with the stress group,the expression of Tyr-tubulin in the hippocampus of the music therapy stress and the control group significantly decreased (P＜0.O1),in contrary,the expression of Acet-tubulin in the hippocampus of the music therapy stress and the control group significantly increased (P＜0.01).However,no significant difference was observed in the frontal cortex and the hypothalamus among the three groups for either Tyr-tubulin or Acet-tubulin (P＞0.05).Conclusions This study indicates that music therapy can increase the release of the 5-HT in the hippocampus and the frontal cortex,and improve the expression of tubulin dynamics of the hippocampus for rats stressed.

OBJECTIVE:To improve the method for the determination of content and related substances of Molsidomine tablet. METHODS:HPLC was adopted to determine the content and related substances,HPLC-MS was adopted for the qualitative degra-dation impurities in destruction test. The column was Waters Symmetry-C18 with mobile phase of 0.05% formic acid- methanol (gradient elution) at a flow rate 1.0 ml/min and 0.2 ml/min (respectively for content and related substances determination,mass spectrometry),the detection wavelength was 210 nm,column temperature was 30℃,the injection volume was 20μl and 5μl(re-spectively for content and related substances determination,mass spectrometry). RESULTS:Each component was well separated un-der chromatographic conditions;degradation impurities were confirmed;the linear range of molsidomine was 4. 0-28.0 μg/ml(r=0.9997);RSDs of precision,stability and reproducibility tests were lower than 1%;recovery was 97.74%-100.70%(RSD=0.49%,n=9). CONCLUSIONS:The method is simple,accurate with good specific and high precision,and can improve the quali-ty control of Molsidomine tablet.

ObjectiveTo observe the effect of Chinese medicinal application at Yongquan (KI1) on hot flush and sweating brought by using GnRH-ain patients with endometriosis.MethodTotally 120 patients diagnosed with endometriosis of stageⅢ~Ⅳ were randomized into a treatment group and a control group. The control group started to receive GnRH-a(Goserelinum) at 3.6 mg/28 d since post-operational day 3, 4 times in total; the treatment group started to receive GnRH-a(Goserelinum) at 3.6 mg/28 d since post-operational day 3, 4 times in total; at the night of taking GnRH-a, the treatment group was also given Chinese medicinal application at Yongquan, a month as a treatment course, successively for 4 courses.ResultThe side effects of using GnRH-a, including hot flush and sweating, were significantly less in the treatment group than that in the control group (P<0.05).Conclusion Medicinal application at Yongquan can significantly reduce the side effects including hot flush and sweating brought by using GnRH-ain patients with endometriosis.

Chronic diseases (ChDs) today constitute the leading cause of morbidity and mortality. Increasing evidence suggests that the production and development of ChDs are associated with amplified inflammation, which is caused by accumulation of advanced glycation end products (AGEs) and advanced lipexidation end products (ALEs).Heating, irradiation, and ionization, in combination with gross over-nutrition, significantly contribute to production of, exposure to and accumulation in the body of AGEs/ALEs. Furthermore, dietary AGEs restriction in animals seemed to be effective to extend life span. This paper elucidates the relationship between food and AGEs and the dietary measures to reduce AGEs/ALEs with an attempt to provide new methods for prevention and treatment of ChDs.

Objective To study the similarities and differences of information needs between cancer patients treated with radiotherapy and chemotherapy, analyze the causes for cancer patients' different choices, and provide reasonable and disease-related information for cancer patients. Methods A total of 102 and 119 cancer patients treated with radiotherapy and chemotherapy relatively were asked to complete the questionnaire (IPQCP+2) and two questions about the total information capacity and content requirements. Results Two groups had different needs of therapeutic information and treatment in IPQCP. Cancer patients treated with chemotherapy needed more widely-ranged information than patients underwent radio-therapy. Conclusions Most of cancer patients treated with radiotherapy and chemotherapy need to be informed of disease-related information, among which the content of therapeutic information and total information is relative to the treatment. Nurses should provide disease-related medical information based on their different needs, as to improve the life quality of cancer patients.

0.05) among the three groups in cell proliferation in 1~10 d cultures and in total protein content in 4~7 d cultures. At 4 and 7 days, ALP activity of MG63 cells cultivated on AD-TNZS disks was significantly higher than that of cells on the other samples(P

Objective To understand occupational stress and burnout status of nurses working for general or cancer hospitals of Xinjiang so as to provide a theoretical basis for the prevention and reduction of occupational stress and burnout of nurses.Methods A total of 497 nurses from a general hospital or a tertiary-level cancer hospital were recruited by using random sampling to learn their occupational stress and burnout.Results Of 497 participants,247 were working for comprehensive hospitals and 250 for cancer hospitals.In comparison with the norm,the nurses of general hospitals showed higher scores in occupational task dimension (t=14.316,P＜0.05) and personal strain dimension (t=25.416,P＜0.05),but lower scores in personal resource dimension (t=7.389,P＜0.05).Meanwhile,the nurses of cancer hospitals were found to have higher scores in occupational task dimension (t=15.840,P＜0.05) and personal strain dimension (t=15.694,P＜0.05),but lower scores in personal resource dimension (t=7.488,P＜0.05) when compared to the norm.Those working in general hospitals had higher scores in emotional exhaustion dimension (t=13.109,P＜0.05) and passive burnout dimension (t=13.198,P＜0.05) than the norm,while lower scores in professional sense dimension (t=17.716,P＜0.05).The nurses of cancer hospitals were observed to show higher scores in emotional exhaustion dimension (t=8.044,P＜0.05) and negative burnout dimension (t=1 1.111,P＜0.05) than the norm,while lower scores in professional sense dimension (t=20.820,P＜0.05).Conclusions Hospital managers should take efforts to control nurses' occupational stress and burnout-related factors so as to enhance their work initiative.

Objective To explore the feasibility of DSCT dual-energy technique in pulmonary mass lesions.Methods A total of 100 patients with pulmonary masses underwent conventional plain CT scan and dual-energy enhanced CT scan.The virtual non-contrast (VNC) images were obtained at post-processing workstation.The mean CT value,enhancement value,signal to noise ratio (SNR),image quality and radiation dose of pulmonary masses were compared between the two scan techniques using F or t test and the detectability of lesions was compared using Wilcoxon test. Results There was no statistically significant difference among VNC ( A ) ( 32.89 ± 12.58 ) HU,VNC (S) ( 30.86 ± 9.60) HU and conventional plain images (35.89 ± 9.99 ) HU in mean CT value of mass ( F =2.08,P ＞ 0.05 ).There was statistically significant difference among VNC ( A ) ( 3.29 ± 1.45 ),VNC (S) ( 3.93 ± 1.49 ) and conventional plain image (4.61 ± 1.50) in SNR ( F =6.01,P ＜ 0.05 ),which of conventional plain scan was higher than that of VNC.The enhancement value of mass in conventional enhanced scan(60.74 ± 13.9)HU and distribution of iodine from VNC (A) ( 58.26 ± 31.99 ) HU was no statistically significant difference ( t =0.48,P ＞ 0.05 ),but there was a significant difference between conventional enhanced scan (56.51 ± 17.94 ) HU and distribution of iodine from VNC (S) (52.65 ± 16.78 ) HU (t =4.45,P ＜ 0.05 ).There was no statistically significant difference among conventional plain scan ( 4.69 ± 0.06 ) and VN C ( A ) ( 4.60 ± 0.09 ),VNC (S)(4.61 ±0.11 ) in image quality at mediastinal window ( F =3.014,P ＞ 0.05 ).The appearance,size,internal features of mass (such as necrosis,calcification and cavity) were showed the same in conventional plain scan,VNC (A) and VNC (S).Of 41 patients with hilar mass,18 patients were found to have lobular and segmental perfusion decrease or defect. Perfusion defect area was found in 59 patients with peripheral lung mass. The radiation dose of dual-energy enhanced scan was lower than that of conventional scan.Conclusion The virtual non-contrast,distribution of iodine and pulmonary virtual perfusion images can be obtained by DSCT dual-energy technique in one scan,which has a potential clinical value in the thorax.

Objective To investigate the dynamic changes of mean platelet volume (MPV) and platelet distribution width (PDW),and to explore the role of MPV and PDW in the prognosis of patients with acute myocardial infarction (AMI).Methods This retrospective cohort study included 312 patients with AMI during 2012 to 2014 in The First Affiliated Hospital of Soochow University.Patients were divided into ST-elevation myocardial infarction (STEMI) group,non ST-elevation myocardial infarction group and low PDW group,high PDW group.Their clinical data and outcomes were analyzed.MPV and PDW were measured successively from admission to day-7 after AMI.The relationship between PDW,MPV and GRACE risk score was further investigated.Results In the STEMI group,the patients were younger (P =0.005),and with higher rates of hyperlipidemia and smoking (P ＜ 0.01).Patients in STEMI group had higher risk of death during hospitalization,compared to NSTEMI (P =0.014).In the high PDW group,the rates of congestion heart failure,cardiogenic shock and Killip ⅣV were higher (P ＜ 0.01;P =0.026;P ＜ 0.01).PDW was significantly associated with mortality of in-hospital,one-year mortality and the risk of re-infarction in one year (r =0.69,P ＜ 0.01;r =0.68,P ＜0.01;r =0.70,P ＜ 0.01).MPV was associated with one-year mortality (r =0.30,P =0.02).Conclusions PDW related to the severity of AMI could predict the risk of in-hospital mortality,one-year mortality and re-infarction.It was helpful to screen out the high-risk patients,so as to make more suitable treatment to improve the prognosis of patients.