Objective To discuss the selection of conveyance and the temperature safeguards during the transport of blood specimens for centralized nucleic acid detection.Methods A total of five chips,which have been set every 10 minuets to record the temperature,have been placed in the Specimen box accordance with the appendix B ofblood transport requirements (WS/T 400-2012).Then,observe the temperature changes in case of ice been placed on both sides,sides and top,sides and bottom,sides and top,bottom of the specimen box respectively.Results In case of ice been placed on both sides of the specimen box,the temperatures were always higher than 10 ℃.In case of ice been placed on both sides and the top of the specimen box,the temperatures were all in range of 2-10 ℃ within 13 hours.In case of ice been placed on both sides and the bottom of the specimen box,only the temperatures of the top were always higher than 10℃.In case of ice been placed on both sides,top and bottom of the box,the temperatures of the bottom were always lower than 2 ℃.Conclusion In case of ice been placed on both sides and top of the box was the most appropriate temperature safeguards during the transport of blood specimens,while in the other cases,the temperatures were lower than 2 ℃,or higher than 10 ℃.

Objective To explore food allergies in children with asthma in urban areas. Methods A total of 1 462 children with asthma who were treated in Mianyang Central Hospital of Sichuan Province from January 2018 to January 2019 were enrolled as the observation group, and 1,828 children who underwent physical examination in the same hospital at the same time were selected as the control group. The types and proportions of common food allergies were summarized and calculated, and the types and proportion of clinical symptoms in the two groups were statistically analyzed. Results A total of 219 children with food allergy were found in the observation group, and the prevalence rate was 14.98%. In the control group, 72 children with food allergy were found, and the prevalence rate was 3.94%. The difference between the two groups was statistically significant (χ²=5.036, P=0.024). The peanut allergy rate in the observation group was significantly higher than that in the control group, while the fruit allergy rate was significantly lower than that in the control group. The differences were statistically significant (P<0.05). The incidence of itch, lip and mucous membrane swelling, sneezing and shock were significantly higher in the observation group than in the control group, while the incidence of skin itching and rash was significantly lower than that in the control group. The differences were statistically significant (P<0.05). Conclusion The prevalence of food allergy in children with bronchial asthma is higher than that in non-bronchial asthma children, and the common food allergies and clinical symptoms of bronchial asthma are significantly different from those of non-bronchial asthma children. The clinicians can make a preliminary diagnosis based on the common food allergies and clinical symptoms of children.