1.The Relationship between Localized Brain Damage and Agraphia
Qiuyou XIE ; Hongyu SUN ; Xiaojia LIU
Chinese Journal of Clinical Psychology 2001;9(1):73-75
Writing behavior is affected by many factors and depends on the functional integrity of the nervous system. Its neuropsychological mechanism remains unknown. The agraphic features involving different parts of brain damage are dissimilar. The neuroanatomic location of agraphia and its possible brain mechanism are reviewed.
2.Effect of compound Fengshining combined with methotrexate on Treg/Th17 immune balance in fibroblast-like synoviocytes of rheumatoid arthritis
Xiaojia LI ; Yingjie ZHAO ; Guangchen SUN
Chinese Journal of Biochemical Pharmaceutics 2015;(6):6-9
10 and Foxp3 expression, reduce the IL-17 and ROR-γt expression and adjust Treg/Th17 immune balance; effect of 0.1 mg/mL FSN +0.1 mg/mL MTX is the most obvious.
3.HIGH EXPRESSION OF RECOMBINANT HUMAN BASIC FIBROBLAST GROWTH FACTOR IN ESCHERICHIA COLI
Ju WANG ; Fenyong SUN ; Xiaojia CHEN ; Ling ZHANG ; An HONG ;
Microbiology 1992;0(02):-
To improve the expression level of non fusion hbFGF in E coli , the coding sequence of human bFGF gene, which had been cloned from primarily cultured human fibroblast, was mutated according to the principle of lowering the GC content and increasing the codon preference After being ligated into pET 3c and transformed into BL21(DE3), the recombinant induced by IPTG Expression level was up to 30% of the total bacterial protein The result indicated that optimizing of the TIR would promote the expression level of recombinant protein
4.Effects of Different Extracts of Rupixiao Granules on Mouse Primary Dysmenorrhea Model
Shuyuan WANG ; Hongyu JI ; Xiaojia SUN ; Linhua WU
China Pharmacist 2016;19(3):434-436
Objective:To research the anti-dysmenorrhea effect of different extracts of Rupixiao granules. Methods:The anti-dys-menorrhea effect of different extracts of Rupixiao granules was studied by writhing test induced by oxytocin in mice. Results:Compared with the model group, the extracts of Rupixiao granules could reduce the writhing times induced by oxytocin significantly (P<0. 05,P<0. 01 or P<0. 001), and the water-soluble extract showed the best effect (P<0. 001) without significant difference when compared with the positive control group(P>0. 05). Conclusion:The water-soluble parts of Rupixiao granules have significant anti-dysmenor-rhea effect.
5.Antibacterial Activities of Cefoperazone-sulbactam Against Gram-negative Bacilli
Yajuan LI ; Pengpeng LIU ; Ping LI ; Weiwei SUN ; Xiaojia HAO
Chinese Journal of Nosocomiology 2006;0(05):-
OBJECTIVE To evaluate the antibacterial activities of cefoperazone combined with sulbactam against Gram negative bacteria,and compare with other antimicrobial agents.METHODS The antibacterial activities of 10 frequently used antibiotics against 1 670 strains of clinical isolated Gram negative bacteria were studied by using agar dilution methods according to the NCCLS 2002.RESULTS The results showed imipenem was the most active tested against Enterobacteriaceae such as Escherichia coli and Klebsiella pneumoniae,followed by cefoperazone-(sulbactam),which had similar activities as imipenem against the non-fermentative strains such as Pseudononas aeruginosa,and Acinetobacter spp,but cefoperazone-(sulbactam) had higher susceptibility to imipenem-(resistant) Stenotrophomonas maltophilia.CONCLUSIONS Cefoperazone-sulbactam has good and broad spectrum(antibacterial) activities especially against Gram negative(bacteria,) and is expected to have a bright prospect in the treatment of severe hospital infections induced by Gram negative bacteria.
6.High and stable expression of an analog of human basic fibroblast growth factor in Escherichia coli
Qiongyu CHEN ; Fenyong SUN ; Xiaojia CHEN ; Qiuling XIE ; Jinhua SUN ; An HONG
Chinese Journal of Pathophysiology 2006;22(2):247-250
AIM: To obtain a high and stable expression analog of human basic fibroblast growth factor by genetic engineering. METHODS: The cysteins 78 and 96 of natural hbFGF polypeptide was substituted with serines by means of site-directed mutagenesis. Using pET- 3c as vector, the mutated polynucleotide was cloned and then transferred into BL21 (DE3)plysS. After induction by IPTG, the analog was obtained and analyzed by SDS - PAGE. RESULTS: After purification the form of soluble mutant increased remarkably but the forms of dimmer and higher multimer were reduced greatly to no more than 8% of the total recombinant protein. By MTT assay, the analog showed the same biological activity. This new analog represented a desirable complementation for native hbFGF to develop pharmaceutical drug in clinical use. CONCLUSION: Substitution of certain amino acids of polypeptide without altering native protein' s bioactivity to get the analog is an effective means to increase stability of foreign protein and its solubility in E. coli.
7.Concepts and Problems of Gait Rehabilitation Robots for Stroke
Huawei LIU ; Huifang WANG ; Jinjie ZHU ; Jiajia YAO ; Yi SUN ; Haofeng SHEN ; Xiaojia ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2013;19(5):493-494
The gait rehabilitation robots for stroke are responding to the development of the understanding of stroke rehabilitation, but still some challenges for application may be faced to, as found in AutoAmbulator. Some advices were dicussed from the view of the practice.
8.Analysis on the cooperative intentions and needs of Chinese and Association of Southeast Asian Nations medical universities/colleges in research
Jing BAI ; Xiaojia LI ; Qiudan SUN
Chinese Journal of Medical Science Research Management 2022;35(3):166-170
Objective:The study aims to gain information on medical research status and cooperative needs of medical universities/colleges from China and Association of Southeast Asian Nations (ASEAN) and provide evidence to guide the regional cooperation and development in medical reserach.Methods:A self-constructed questionnaire survey was adopted to collect information on the cooperative intentions, expertise and challenges of the 43 member institutions of China-ASEAN University Consortium on Medicine and Health in medical research.Results:In total, 20 Chinese members and 10 ASEAN members completed the survey. The study found that both Chinese and ASEAN medical universities/colleges were particularly interested in cooperation in academic conferences, multi-center clinical trials and joint research projects /flagship projects, followed by large cohort studies and medical journal/publication. In terms of research priority, both Chinese and ASEAN institutions were very interested in big data, followed by cancer precision medicine; while ASEAN members were more interested in natural drugs and dengue than Chinese ones.Conclusions:Both Chinese and ASEAN medical universities/colleges had strong interest in international cooperation in medical research. They should further explore the advantages and characteristics of both sides in medical research based on the interests of two sides, adopt appropriate scientific research cooperation methods, and establish sound scientific research cooperation mechanism so as to deepen cooperation in medical research, promote the development and improve the outcome of regional medical research, and facilitate the building of a community of common health for mankind.
9.A dynamic observation of pathologic and ultrastructural changes of perihematoma in intracerebral hemorrhage patients
Fuqiang GUO ; Xiangrong SUN ; Yousong YANG ; Yuchuan XU ; Longyi CHEN ; Linglin DONG ; Yongsheng WEI ; Yulan HUANG ; Hongbin SUN ; Xiaojia LI ; Hong YANG
Chinese Journal of Nervous and Mental Diseases 2007;33(1):13-17
Background In recent years,some researches had been conducted on the pathologic changes of the secondary injury of perihematoma in animal experiments,but only a few studies had been done on the dynamic pathologic and ultrastructural changes of the perihematoma in ICH patients. The unique contribution of our study is to investigate the dynamic pathologic and ultrastructural changes of the perihematoma in ICH patients and provide significant insights into how the pathophysiology and ultrastructures changed after ICH.Methods The written informed consents were obtained from the ICH patients or their relatives. 30 patients (the supertentorial hemotoma volume>30 mi and the cerebellar hemotoma volume >10 mi) were divided into 8 groups according to the time passed after ICH:<6 h (6 patients), 6 ~ 12 h (7 patients), 12 ~24 h (5 patients), 24~48 h (3 patients), 48 ~72 h (3 patients), 3 ~4 days group (3 patients), 5 days group (2 patients) and 8 days group ( 1 patient) and subjected to craniotomy for hemotoma evacuation. During the operation for the hemotoma's evacuation, a small amount of tissues that must be removed, which located at 1 cm near the hematoma, were taken as experimental groups; And the same tissues of 7 patients (<12 h), which were far from the hemotoma on the operational way, were taken as control group. The pathologic and ultrastructral changes were observed.Results The tissues of the control group were almost normal while the damages of the tissues from the experimental groups were slight in <6 h groups, more severe after 6h and got to the maximum between 24 ~48 h , recovered gradually after 72 h, became similar to the 6 ~ 12 h group on 5 th day, got better on 8 th day and resembled the 6 h group.Conclusions The injury of the perihematoma occurred in early stage, reached the peak level between 24 and 48 hours after ICH; which was consistent to the clinical nervous functional deficits in the ICH patients.
10. Cross-cultural adjustment of successful aging scale and its reliability and validity in urban elderly
Pei TONG ; Jingjing ZHOU ; Yajing ZHENG ; Qingzhuo REN ; Yunyi LIANG ; Qiaoqiao SHEN ; Xiaojia SUN ; Yulin GAO
Chinese Journal of Behavioral Medicine and Brain Science 2019;28(11):1030-1036
Objective:
To revised the Taiwan version successful aging inventory (SAI), and verify its reliability and validity in urban elderly in mainland China.
Methods:
SAI was adjusted by culture and revised by pre-test to form a formal questionnaire. A sample of 600 elderly people in Guangzhou was chosen for a face-to-face questionnaire survey through convenient sampling. Half of the sample (