1.External Quality Assessment of Clozapine for Therapeutic Drug Monitoring in Guangdong Province
Yongshuang GAO ; Jiaxuan ZHANG ; Xiaojia NI ; Wei GUO
China Pharmacist 2016;19(9):1791-1793
Objective:To implement the external quality assessment ( EQA) of clozapine for therapeutic drug monitoring ( TDM) among psychiatric hospitals in Guangdong. Methods:The parameters of HPLC used by the hospitals were understood through question-naires. The EQA of clozapine for TDM was implemented by distributing the quality control serum. Results:The qualified rate of cloza-pine in the quality control serum at low, medium and high concentrations for TDM was 66. 67%, 66. 67% and 83. 33%, respectively. Conclusion:It is necessary to implement EQA of clozapine for TDM in Guangdong.
2.Ubiquitin and its role in the tumor
Xiaojia GAO ; Hui YANG ; Lin WU ; Fuxiang ZHOU ; Yunfeng ZHOU
Journal of International Oncology 2015;(3):188-191
Ubiquitin-proteasome pathway is one of the primary pathway in intracellular protein degrada-tion,therefore the ubiquitin conjugating enzyme D3(UbE2D3)which involved in the ubiquitin mineralization process can affect the biological effects accordingly to affect some protein and nucleic acid content and activity. The function that participate in modification and degradation of some cancer factors is vital in tumor cells,and followed by tumor biological behavior changing. Researches show that UbE2D3 correlates with human telomer-ase reverse transcriptase( hTERT),radiation sensitivity,aggressive,etc in breast cancer.
3.Radiosensitization of clioquinol and zinc in human cervical cancer cell lines
Shan LU ; Yuan KE ; You WANG ; Hong ZHAO ; Xiaojia GAO ; Haijun YU ; Fuxiang ZHOU
Chinese Journal of Radiological Medicine and Protection 2016;36(4):241-245
Objective To investigate the radiosensitization effects of the combination treatment of clioquinol (CQ) and zinc on human cervical cell line HeLa in vitro.Methods Cells were divided into the 4 groups:controls,drug,radiation,and combined drug and radiation group.Cytotoxic effect of CQ and zinc on cell viability was determined by CCK-8 assay.Radiosensitization effect of CQ and zinc on HeLa cells was detected by colongenic assay,and the single-hit multi-target model was used to stimulate the doseresponse curve of survival and to calculate radiosensitization parameters.The cell cycle and apoptosis of HeLa cells were analyzed with flow cytometry.Luciferase reporter assay was used to study NF-κB activity of HeLa cells.Results The combination of CQ and zinc inhibited cell growth in a dose-dependent manner (F =188.00,P < 0.01).The mean lethal dose was 3.16 and 2.04 Gy for radiation group and combined drug and radiation group,respectively,and hence the SER was 1.55.Compared with the radiation group,the ratio of G2-phase cells in the combined drug and radiation group decreased(t =10.39,P < 0.05),the apoptosis rate increased at 24 h post-irradiation (t =5.64,P < 0.01),and the NF-κB activity decreased (t =21.42,P < 0.05).Compared to the control group,the NF-κB activity increased in the radiation group(t=6.23,P<0.05),but decreased in the drug group(t =12.48,P<0.05).Conclusions The combination of CQ and zinc could increase the radiosensitivity of HeLa cells by decreasing the ratio of G2-phase cells,increasing apoptosis and the inhibiting of NF-κB activity.
4.Liensinine promotes apoptosis of bladder cancer 5637 cells and enhances Caspase-7 expression and activation
Bin XU ; Tianyu ZHANG ; Ning TAN ; Qi YAN ; Lingli XU ; Xiaojia LIU ; Li GAO
Tianjin Medical Journal 2015;(7):724-727
Objective To study the effects of Liensinine on apoptosis of 5637 cells, and its mechanism thereof. Meth?ods CCK-8 method and the colony formation test were used to detect cell viabilities, and then inhibition rates were calcu?lated. Flow cytometry was used to detect the effects of Liensinine on apoptosis of 5637 cells. Western blot assay was used to detect Caspase-7 protein expression. Results CCK-8 assay and colony formation test indicated that Liensinine inhibited the cell proliferation significantly. Results of flow cytometry indicated that Liensinine induced early apoptosis of 5637 cells. Western blot assay showed that Liensinine improved the expression of Caspase-7 and enhanced the activation of Caspase-7 in 5637 cells. Conclusion Liensinine could inhibit the proliferation of 5637 cells, induce early apoptosis, which may be re?lated with the enhanced expression of Caspase-7 and its activation.
5.Investigation on the status quo of social alienation in maintenance hemodialysis patients and its correlation with self disclosure and social support
Jinxue QIN ; Xiaojia ZONG ; Honghua GAO
Chinese Journal of Practical Nursing 2023;39(2):119-125
Objective:To investigate the status of social alienation in maintenance hemodialysis (MHD) patients, and analyze its correlation with self disclosure and social support.Methods::From May 2019 to March 2021, 156 MHD patients admitted to Nanyang Central Hospital were selected as the research objects by using convenient sampling method. A cross-sectional survey was conducted by using general information questionnaire, general alienation scale, pain disclosure index scale and social support scale.Results:A total of 176 questionnaires were issued, with an effective recovery rate of 88.64%(156/176). The social alienation score of 156 patients with MHD was (41.57±4.89) points, which was at the upper-middle level and negatively correlated with the total scores of the Distress Disclosure Index and the Perceived Social Support Scale ( r=-0.526, -0.284, all P<0.01). The results of multiple linear regression analysis showed that age, educational level, and per capita monthly household income were the main factors affecting social alienation ( β=0.199, -0.361, -0.290, all P<0.01). After controlling for the above factors, self disclosure and social support independently explained the variance of social alienation in MHD patients increased by 20.2% ( β=-0.440, -0.200, all P<0.01). Conclusions:MHD patients have a high sense of social alienation. Medical staff should pay attention to patients who are older, less educated, and have low family income. Self disclosure and social support are modifiable variables, so medical staff can reduce their social alienation and improve the quality of life by training patients on self disclosure and improving the level of social support.
6. Cross-cultural adjustment of successful aging scale and its reliability and validity in urban elderly
Pei TONG ; Jingjing ZHOU ; Yajing ZHENG ; Qingzhuo REN ; Yunyi LIANG ; Qiaoqiao SHEN ; Xiaojia SUN ; Yulin GAO
Chinese Journal of Behavioral Medicine and Brain Science 2019;28(11):1030-1036
Objective:
To revised the Taiwan version successful aging inventory (SAI), and verify its reliability and validity in urban elderly in mainland China.
Methods:
SAI was adjusted by culture and revised by pre-test to form a formal questionnaire. A sample of 600 elderly people in Guangzhou was chosen for a face-to-face questionnaire survey through convenient sampling. Half of the sample (
7.Level of self-compassion among residents and related influencing factors
Xiaodan MA ; Xiaojia LU ; Ting SUN ; Yuan GAO
Chinese Journal of Medical Education Research 2023;22(9):1426-1430
Objective:To investigate the level of self-compassion among residents and related influencing factors, and to help them improve self-compassion ability, achieve learning goals, and increase the degree of satisfaction with learning.Methods:In July 2022, the random sampling method was used to select 330 residents (postgraduate students majoring in clinical medicine after national unified recruitment) from two grade A tertiary hospitals in Shenyang of Liaoning province in China, and Self-Compassion Scale was used for investigation. SPSS software was used to analyze the degree of satisfaction with industry, learning pressure, the balance between learning and life, and occupational burnout among residents and their association with the level of self-compassion through the chi-square test, the one-way analysis of variance, and the multilevel regression analysis.Results:The residents had a mean self-compassion score of (40.90±6.81), and the residents of the second grade had a lower level of self-compassion than those of the other grades. Major satisfaction and balance between learning and life were positively correlated with self-compassion, and occupational burnout was negatively correlated with self-compassion.Conclusion:There is a relatively insufficient level of self-compassion among residents, and improving self-compassion level may effectively regulate occupational burnout, professional satisfaction, and the balance between learning and life, thereby helping to improve learning and mental health.
8.Discussion on the Inhibitory Mechanism of Lanthanum Chloride on Vascular Smooth Muscle Cell Calcification Induced by High Phosphorus Based on NF-κB Signaling Pathway
Chao GU ; Lulu ZHAO ; Gang LI ; Yuan GAO ; Shengnan WANG ; Xiaojia LI ; Xiaorong YUAN ; Qiwen WANG ; BAOLECHAOLU ; Ruilan HAN
China Pharmacy 2021;32(20):2458-2466
OBJECTIVE:To discuss the inhibitory effect of lanthanum chloride on the calcification of vascular smooth muscle cells(VSMCs)induced by high phosphorus and its mechanism. METHODS :On the basis of screening the action concentration and time of lanthanum chloride by MTT method ,human VSMCs were divided into control group (1 mmol/L phosphorus solution ), lanthanum chloride high concentration control group (1 mmol/L phosphorus solution+ 60 μmol/L lanthanum chloride),model group (3 mmol/L phosphorus solution ),sodium chloride group (3 mmol/L phosphorus solution+ 180 μmol/L sodium chloride),nuclear factor κB(NF-κB)signaling pathway agonist+lanthanum chloride group (3 mmol/L phosphorus solution+ 1 μg/mL lipopolysaccharide+ 60 μmol/L lanthanum chloride),positive control group (3 mmol/L phosphorus solution+ 100 μmol/L sodium pyrophosphate),and lanthanum chloride low ,medium,and high concentration groups (3 mmol/L phosphorus solution+ 15,30,60 μmol/L lanthanum chloride). Alizarin red S staining and Von Kossa staining were used to detect cell calcification in each group after treated with phosphorus solution for 6 d and relevant medicine for 2 d. Western blot assay was used to detect the protein expression of TNF-α receptor associated protein 6(TRAF6),nuclear factor κB inhibitor protein α(IκBα),NF-κB p65,bone morphogenetic protein 2 (BMP-2),smooth muscle 22 α(SM22α)and Runt related transcription factor 2(Runx2). Real-time fluorescence quantitative polymerase chain reaction was used to detect mRNA expression of TRAF 6,IκBα,BMP-2,SM22α and Runx2. RESULTS : Compared with control group ,no cell calcification was observed in the lanthanum chloride high concentration control group ,while obvious cell calcification and significant increase of OD value were observed in model group and sodium chloride group (P< 0.01);protein and mRNA expression of TRAF 6 and BMP- 2 in cytoplasm as well as mRNA expression of Runx 2,protein expression of NF-κB p65 and Runx 2 in nucleus were significantly increased (P<0.01);protein and mRNA expression of IκBα and SM22α as well as protein expression of NF-κB p65 in cytoplasm were significantly decreased (P<0.01). Compared with model group,cell calcification was significantly improved in lanthanum chloride groups and positive control group ,while OD values were significantly reduced ;the expression levels of the above-mentioned protein and mRNA were reversed to varying degrees (P<0.05 or P<0.01). Compared with lanthanum chloride high concentration group ,obvious cell calcification was observed in NF-κB signaling pathway agonist + lanthanum chloride group ,and OD value was significantly increased ;the above indexes were significantly reversed in cytoplasm and nucleus (P<0.05 or P<0.01). CONCLUSIONS :Lanthanum chloride can inhibit the calcification of VSMCs induced by high phosphorus ,and its mechanism may be related to the inhibition of NF-κB signaling pathway activation.