Aim To investigate the role of HIF-1 αin PC1 2 cell injury induced by quinolinic acid.Methods PC1 2 cells were treated with quinolinic acid at the do-ses of 2.5,5 and 1 0 mmol·L -1 ,the cell viability was determined by MTT reduction assay and LDH as-say,the intracellular levels of oxygen species was measured by assessing SOD and MDA levels,cell ap-optosis was determined by Hoechst 33258 staining,the intracellular distribution of HIF-1 αwas examined by HIF-1 αimmunostaining,and the expressions of HIF-1 α,Akt,p-Akt,Bcl-2 and Bax were determined by im-munoblotting analysis.Results Quinolinic acid in-duced cell injury in PC1 2 cells in a dose-dependent manner,and potentiated oxygen radical production and cell apoptosis.In addition,quinolinic acid enhanced HIF-1 αexpression and accumulation in nuclei.The p-Akt expression and Bax/Bcl-2 ratio was increased by quinolinc acid in PC1 2 cells.Conclusions HIF-1 αand Akt mediate qunolinc acid-induced cell apoptosis in PC1 2 cells.And cellular oxidative stress may con-tribute to the injury as well.

Objective: To determine the impact of job burnout on perception of risk from adverse events among clinical nurses. Methods: A total of 480 clinical nurses were recruited from four Secondary B-class hospitals in Tianjin by convenience sampling method,between December 2017 to December 2018. They were investigated with Clinical Nurses Adverse Events Risk Perception Structure Scales and Maslach Burnout Inventory. The relationship between job burnout and perception of risk from adverse events was analyzed. Results: The total score of clinical nurses adverse events risk perception structure scales was 304.60±59.20. There were significant negative correlations between score of job burnout and perception of risk form adverse events among clinical nurses (r=-0.418--0.334, P<0.01). Regression analysis showed that the factor scores of job burnout such as emotional exhaustion and personal accomplishment could independently explain 42.1% of the variance for perception of risk from adverse events among clinical nurses after controlling demographic variables. Conclusions Job burnout can predict the perception of risk from adverse events of clinical nurses, suggesting that nursing managers should reduce the job burnout to improve perception of risk from adverse events among clinical nurses.