Objective To characterize the nasal resonance of children with spastic cerebral palsy by comparing it with that of ordinary school-age children.Methods The mean nasalance scores (MNSs) of 90 normal school-age children and 62 school-age children with spastic cerebral palsy were measured and compared.Results (1) Age has significant effects on the MNS of/a/,/i/and/m/ in ordinary children,but has almost no effect on the MNS of/u/.The MNS of/a/,/i/,/u/ and/m/ in children with spastic cerebral palsy does not change with age.(2) Sex only has a significant relationship with the MNS of/i/ in ordinary children,but does not significantly predict the other MNSs.(3) The MNS of/a/ of children with spastic cerebral palsy is significantly lower than that of ordinary children,and their MNS of/i/ and /u/ is significantly greater than those of ordinary children.Conclusions The MNS of /a/,/i/and /u/first increases and then decreases with age in ordinary children,while the MNS of/m/ increases gradually.Children with spastic cerebral palsy did not show the same trends and demonstrated a state of retardation of nasal resonance.Children with spastic cerebral palsy are more likely to display hypernasality than ordinary children.

Medical service data center could play a pivotal role in the assessment and improvement of patient safety,assist of medical quality management,providing guidance for patients,promoting the development of biological bank,by collection,storage,maintenance and analysis of medical data.Even though the building of medical service data center in China is relatively late compared with developed countries,however,China has made some achievements in guiding medical service and medical service management.

OBJECTIVE: To establish methods for the identification of Rhizoma Chuanxiong and determination of Ferulic acid( FA) in Ganning granula. METHODS: The identification of Rhizoma Chuanxiong was carried out by TLC and the determination of FA was by performed by HPLC. FA was separated on Kromasil ODS-1 C18 column with water-methanol-acetic acid ( 30∶ 69. 5∶ 0. 5) as mobile phase. The detection wavelength was 322nm, the column temperature was set at room temperature and the flow rate was 1. 0mL? min-1. RESULTS: The TLC spots of the sample presented the same color as its counterparts of Rhizoma Chuanxiong standard and FA reference substance at the corresponding sites. The linear range of FA was 0. 010 12 ～ 0. 151 80? g( r=0. 999 9, n=8) . The average recovery was 97. 39% ( RSD=1. 97% ) . CONCLUSION: The methods of identification and content determination were rapid, accurate, specific and reproducible, and which are suitable for the quality control of Ganning granula.

Objective To observe the protective effect of spironolactone on heart of hypertensive SD rats.Methods 30 male SD rats were randomly divided into the control group,hypertensive group(HS group),low spironolactone dose group(HSL group)and high spironolactone dose group(HSH group).The animals except the control group were treated with L-NAME 50 mg/kg/d.Then the animals of the HS group were fed with 1% sodium chloride water solution.Those of the HSL and HSH groups were treated same but the former added with spironolactone 20 mg/kg/d,the later added with spironolactone 100 mg/kg/d.8 weeks late,the animals were killed and hearts were taken out to observe following items:myocardial cross-sectional area,ratio of per-arteriolar fibrous area/arteriolar luminal area of arterioles,ratio of collagen area/total myocardial area,myocardial necrosis and inflammation.Results The myocardial cross-sectional areas in the HS group were significantly more than those in other 3 groups(P<0.01).Collagen ratios of the HS group were significantly higher than others(P<0.01).The ratios of arteriolar wall area versus luminal area in the HS group were significantly higher than those of control and HSH groups(P<0.01).Myocardial injuries in the HS group were more severe than those of other 3 groups.There were no differences between HSL and HSH groups.Conclusion Spironolactone has protective effect on heart of hypertensive SD rats.

Matrine is a traditional Chinese medicine extraction which can inhibit the proliferation, migration and apoptosis of tumor cells. Cell signal transduction affects the tumor biological behavior and growth condition. Except for the widely research of MAPK, JAK-STAT, NF-κB signal transduction pathways, the latest signaling pathways about matrine are Wnt/β-catenin signaling pathway and mTOR signaling pathway. These pathways are mainly activated by the phosphorylated key proteins to alleviate inflammatory reaction and inhibit the growth of tumor cells and tumor biological behaviors. The research of matrine by cell signaling pathways can provide molecular targeted therapy with theoretical basis. The relationship among different signaling pathways related to the anti-tumor effect of matrine is worth studying deeply in the future.

Objective To observe the effects of swimming on school-age children with cerebral palsy. MethodsHalliwick swimming method was used in swimming group (n=16), while the control group (n=15) received routine training. Before and after training, they were assessed with the Gross Motor Function Measure (GMFM-88) and the Berg Balance Scale. ResultsMost of the swimming group mastered swimming skills. The total scores of GMFM-88 and the Berg Balance Scale scores improved significantly in the swimming group (P<0.05), but not significantly in the control group (P>0.05) after training. ConclusionHalliwick technique is an effective way to learn swimming for school-age children with cerebral palsy. There is certain effect of swimming on motor function, especially on the balance functions.

Objective To study the relationship between tyrosine kinase receptor B (TrkB)expression and anoikis-suppression and invasion in OVCAR3 ovarian cancer ceils. Methods The expression of TrkB mRNA in OVCAR3 ovarian cancer cells under two culture conditions :adhesive cells and ceil-spheroids were evaluated by RT-PCR and real-time PCR.The relationship between TrkB expression and anoikis-suppression of OVCAR3 ovarian cancer ceils was examined by RNA interference (RNAi) technic,anchorage independent culture and fluorescence-activated ceil sorting analysis.The difference in invasion and metastatic ability of OVCAR3 cells under two culture conditions and with or without TrkB silenced by small interfering RNA (siRNA) was investigated by matrigel invasion assay and in vivo studies.Results The expression of TrkB mRNA was highest in OVCAR3 ovarian cancer ceils,0.0240 ~ 0.0017,compared with the other three cell lines,0.0030±0.0006,0.0027±0.0009 and 0.0087±0.0003 respectively,andthe expression in OVCAR3 multicellular spheroids was significantly higher than that in ceils under monolayer adhesive culture,0.0437±0.0021 versus 0.0240±0.0017 (P＜0.01) . TrkB mediated anoikissuppression in OVCAR3 ovarian cancer ceils.OVCAR3 multiceilular spheroids had a higher invasion ability than OVCAR3 cells under monolayer adhesive culture,and the penetrating cells of the two groups were 71.8± 0.8 and 47.7±0.8 respectively (P＜0.01 ).The metastatic ability of OVCAR3 cells was attenuated when TrkB was silenced,and the volume of the tumors developed by OVCAR3 adhesive cells and OVCAR3 adhesive cells with TrkB silenced were (16.3±4.7) mm3 and(6.0±1.4) mm3 respectively (P＜0.01).Conclusion As an anoikis-suppressur,TrkB may increase the invasion and metastasis of OVCAR3 ovarian cancer cells.

Objective To analyze the expression feature and function of microRNAs in exosomes secreted by leukemia cells (LCEX). Methods The mice leukemia cell line L1210 was taken as the example, and LCEXL1210 was obtained by isolating supernate of L1210 cells through density gradient centrifugation. MicroRNAs isolated from LCEXL1210 were analyzed by microarray analysis, compared with miRNA from L1210 cell line, and then some of miRNAs with different expression were verified by real-time PCR and were analyzed by Gene Ontology (GO) database. Results The number of miRNAs identified in LCEXL1210 was 1 044, and that in L1210 cell line was 872. The number of shared miRNAs between LCEXL1210 and L1210 cell line was 732, accounting for 70.1 % of LCEXL1210 and 83.9 % of L1210 cell line, respectively, which indicated that 70 % of LCEXL1210 was derived from the parental cells. Interestingly, 312 miRNAs in LCEXL1210 were found to be underrepresented in the parental cells, indicating their specificity in LCEXL1210. Some miRNAs were significantly highly expressed in LCEXL1210 compared with those in L1210 cell line, including miR-16-1, miR-210, miR-195 and so on, which showed that miRNAs isolated from LCEXL1210 were differentially expressed with those from the parental cells. Some differentially expressed miRNAs from LCEXL1210 were verified by real-time PCR, and then were analyzed by GO database, which demonstrated that these highly expressed miRNAs participated in the processes of various biological function and signal transduction. Conclusions MiRNAs isolated from LCEXL1210 show a high similarity to miRNAs isolated from L1210 cells, whereas of which one-third are specific. The highly expressed miRNAs participate in the processes of various biological function and signal transduction.

Objective To analyze the voice quality of children post operation for complete cleft palate through subjective and objective evaluation and explore the correlation between them. Methods Electroglottography analysis was used in the natural sound/a/of 30 postoper-ative children with complete cleft palate and 34 normal children, and the difference was compared. The natural prolonged sound/a/was as-sessed with GRBAS (grade, roughness, breathiness, asthenia, strain) in the children with complete cleft palate, and the correlation between the subjective and objective results was analyzed. Results F0, harmonic to noise ratio (HNR) were less, and the shimmer, jitter, normalized noise energy (NNE) and nasalance was greater in postoperative children with complete cleft palate than in normal children. For the children with complete cleft palate, G and R mainly rated as levels 1 and 2, B also rated as levels 1 and 2 in less than half of the children. There were low or very low correlation between all subjective and objective results, excepted G, R, B and F0, which showed moderate correlation. Con-clusion The vocal cords vibrate slowly and irregularly in postoperative children with complete cleft palate, suggesting mild and moderate voice disorder, and they sound hoarsely with roughness and breathiness. There are poor correlations between the subjective and objective as-sessment, and need to be used in combination for the children with complete cleft palate.

Objective To evaluate the prevalence and associated risk factors of stress urinary incontinence (SUI) in adult women in Xinjiang.Methods In the cross-sectional study,3403 Uygur women aged over 20 years were interviewed through a questionnaire of International Consuhation on Incontinence Questionnaire Female Lower Urinary Tract Symptoms Module (ICIQ-FLUTS) and the adult questionnaire were used in our research.All parts of the content were according to the characteristics of women in Xinjiang and the purpose of our research.The risk factors were studied by Logistic regression analysis.Results The prevalence of urinary incontinence (UI) was 4l.96％ (1428/3403) and 28.21％ (960/3403) of SUI in Uygur women.In multivariate logistic regression analysis,the risk factors of SUI are body mass index (OR =1.672,95％ CI:1.082-2.584),parity (OR =5.092,95％ CI:3.889-6.666),neonatal birth weight (OR =5.623,95 ％ CI:3.335-9.480),the mode of delivery (OR =2.247,95 ％ CI:1.634-3.090),the lateral episiotomy (OR =4.448,95％ CI:3.112-6.357),menopause (OR =5.145,95％ CI:3.613-7.328),chronic pelvic pain (OR =3.869,95％ CI:1.051-14.250),pelvic organ prolapse (OR =3.501,95％ CI:2.508-4.887).Conclusion The incidence of SUI is related with multiple factors,especially with obesity and the obstetric factor.