It was found that there was a significant increase of the number of circulating leucocytes 2h after a subcutaneous injcetion of isoprenaline and severe myocardial necrosis occurred after consecutive injections for 3 d.Cytoxan-induced depletion of leucocytes resulted in an obvious decrease of the percentage of myocardial necrosis.In the control.the neutrophilic phagocytosis was enhanced and their release of acid phosphatase elevated greatly,which indicates that the neutrophils are in an activated state.After the.neutrophils were inhibited with dexamethasone or indomethacint significant reduction of the contents of prostaglandin E2 and malondialdehyde and an increase of superoxide dismutase activty in the myocardium were observed and consequently myocardial necrosis was greatly alleviated.Our findings suggest that neutrophils might play an important role in the pathogenesis of myocardial infarction through the release of lysosomal enzymes and the production of oxygen-derived free radicals and certain metabolites of arachidonic acid,and proper regulation of the neutrophilic functions could exert prophylactic effects on myocardial infarction.This might be a promising direction to look for more effective drugs to treat myocardial infarction.

Aim Curcumol and Zedoary Turmeric Oil (ZTO) were prepared to liposome, which were used to study the anti-hepatoma effect, and its mechanism that was expected to provide theoretical support for the clinic anticarcinomal use of Zedoray Rhizome Abstracts.Methods Curcumol and ZTO were prepared to liposome by mechanical diffusion and ultrasonic techniques.Proliferation of HepG2 cell in vitro was observed with curcumol and ZTO by MTT assay. Apoptosis of cells was observed by staining with Hoechst 33258/PI and laser confocal scanning microscopy (LCSM). Expression of COX-2 mRNA and VEGF mRNA of HepG2 cell was investigated by RT-PCR.Results Envelopment ratios of curcumol liposome and ZTO liposome were 86.2% and 74.5%,respectively.Curcumol and ZTO inhibited proliferation of HepG2 cell by MTT assay.Compared with control group, Curcumol and ZTO increased the apoptosis rate of HepG2 cell significantly (P

Objective To develop a nursing pathway outlined the reasonable timing for care of paraplegia patients at rehabilitation stage. Methods Literature and patient records were reviewed to draw up a clinical nursing pathway. It was modified and finalized after 2 rounds of Delphi's expert-consultation. Results and Conclusion The draft was responded by 100% of the experts, with a specialist authoritative coefficient of 0.87. The text for the clinical nursing pathway was then completed.

目的分析脊髓损伤患者的心理特点,指导临床心理护理。方法采用焦虑自评量表(SAS)及抑郁自评量表(SDS)对86例脊髓损伤住院患者进行评定,根据得分高低分别归纳出焦虑和抑郁的主要表现,并制定有针对性的心理护理计划。结果与结论74.4%的患者有焦虑情绪,77.9%有抑郁情绪。焦虑的主要表现为夜间睡眠不好、常常要小便、手脚常常湿冷、不容易安静坐着、觉得会发生什么不幸等,抑郁的主要表现为性功能障碍、做事情感到困难、不容易下决定、生活无意义、吃饭比平时减少等。脊髓损伤患者普遍存在抑郁及焦虑情绪 ,应有针对性地进行心理康复护理。

目的观察促进截瘫患者不良情绪转化的相关因素。方法对86例住院截瘫患者进行日常生活能力评定和焦虑、抑郁量表评定,根据评定结果制定康复训练计划,同时开展心理护理;3个月后进行第2次评定。结果约80%的入院患者有不同程度的焦虑和抑郁情绪,经康复训练和心理护理后,患者的焦虑和抑郁情绪均有不同程度减轻。此外,焦虑及抑郁情绪的轻重与病程长短无关(P>0.05),可促进截瘫患者不良情绪转化的相关因素为适宜的环境、日常生活能力提高、良好的心理护理和及时有效的健康教育。结论了解促进截瘫患者不良情绪转化的相关因素有助于有针对性地对患者实施心理护理,有利于患者回归家庭、回归社会。

AIM: To explore the possible impact of hydrogen sulfide(H2S) donor-sodium hydrosulfide(NaHS) on endothelin-1(ET-1) and connective tissue growth factor(CTGF) expressions in rats with pulmonary hypertension induced by high pulmonary blood flow.METHODS: Thirty-two male SD rats were randomly divided into 4 groups: shunt group,shunt+NaHS group,sham group and sham+NaHS group.Rats in shunt group and shunt+NaHS group were subjected to an abdominal aorta-inferior vena cava shunt to create an animal model of high pulmonary flow.After 11 weeks of experiment,rat systolic pulmonary artery pressure(SPAP),lung tissue H2S,plasma ET-1 concentration and lung tissue ET-1mRNA expression,as well as pulmonary artery CTGF protein expression were detected.RESULTS: After 11 weeks of experiment,SPAP,lung tissue ET-1mRNA,plasma ET-1 as well as pulmonary artery CTGF expressions were increased markedly,respectively,whereas H2S in lung tissue decreased significantly in rats of shunt group as compared with that in sham group(all P

A strain producing pullulanase was isolated from soil and mutated by UV and EMS with the increase of pullulanase activity from 1.6 to 5.4 u/mL. The fermentation conditions and medium were studied and optimized. The enzyme activity can reach 8.8 u/mL under the optimal fermentation conditions and medium. The optimal pH and temperature for enzyme are 4.6 and 75℃ respectively. It shows a high stabiliby during pH4.0～8.0 at 55℃.

A strain producing pullulanase was isolated from soil and mutated by UV and EMS with the increase of pullulanase activity from 1.6 to 5.4 u/mL. The fermentation conditions and medium were studied and optimized. The enzyme activity can reach 8.8 u/mL under the optimal fermentation conditions and medium. The optimal pH and temperature for enzyme are 4.6 and 75℃ respectively. It shows a high stabiliby during pH4.0～8.0 at 55℃.

Objective To explore the effect of propofol and etomidate on anesthesia induction in pediatric surgery, and to provide evidence for the future use of anesthesia in pediatric surgery.Methods120 cases of pediatric surgery patients were prospectively selected pediatric in our hospital from May 2013 to January 2015 as the research object, randomly divided into 2 group respectively, control group and observation group, 60 cases in each group;the control group anesthesia was induced with etomidate, observation group anesthesia was induced with propofol, and the anesthetic effect were observed in the two groups, recovery and adverse reaction in the induction period of anesthesia.ResultsThe patients in the observation group anesthesia satisfaction scores were significantly higher than the control group (P<0.05) and the observation group;the recovery time and the time of leaving room were significantly shorter than the control group (P<0.05);in the observation group, respiratory depression, restlessness, throat and bronchial spasm, adverse reactions such as nausea and vomiting were significantly lower than the control group (P<0.05).ConclusionPropofol is more suitable for pediatric surgery than etomidate, and anesthesia effect is remarkable, fast recovery, worthy of clinical application and promotion.

Objective To investigate the safety and efficacy of sufentanil in pediatric surgery.Methods80 cases of pediatric surgery were prospectively selected pediatric methods in The Third People's Hospital of Xining from July 2013 to January 2015 as the research object, the patients were divided into two groups, control group and observation group, 40 cases in each group.The control group received remifentanil,observation group were given sufentanil, mean arterial pressure(MAP)and heart rate(HR)30min and 5min at each time point after extubation and 5min based value children after entering the operation room after induction were compared.The sedation scores of 5min, 15min and 30min after tracheal intubationwere recorded..ResultsThe pain scores in the observation grouppostoperative T4, T5, T6 each time were significantly higher than the control group (P<0.05);MAP and HRin the observation group at T0, T1, T2, T3 had no significant differencecompared with control group, MAP during T4 in the observation group was significantly higher than the control group (P<0.05);and HR in the observation group in the period of T4 was significantly lower than the control group (P<0.05).ConclusionThe application of sufentanil in pediatric surgery is effective and safe.