Objective To investigate human esophageal cancer Eca109 cells apoptosis induced by resveratrol.Methods Eca109 cells were treated with difierent concentrations of resveratrol and the change of morphology was observed by inverted microscope and transmission electron microscope in different time;The cell growth inhibitory rate of resveratrol on the proliferation of Eca109 cells were evaluated in vitro by MTT assay.Flow cytometry (FCM)analysis was used to determine the apoptosis of Eca-109 cells.Results Resveratrol was able to inhibit the growth of Eca109 cells,and the suppressive effect was increased with treating concentration and treating time.Resveratrol inhibited the growth of esophageal cancer Eca109 cells in a dose-and time-dependent manner and induced the apoptosis in esophageal cancer.Conclusion Resveratrol could inhibit the proliferation of Eca-109 cells and block the cell cycle at G0/G1.And in the end resveratrol could induce the apoptosis of Eca109 cells.

Studies in recent years have demonstrated that there is close relationship between estrogen and aneurysmal subarachnoid hemorrhage. Estrogen affects the occurrence and development process of intracranial aneurysm through multiple pathways, thereby reducing the incidence of aneurysm rupture. Estrogen also alleviates vasospasm after subarachnoid hemorrhage.Identifying the effects and their mechanisms of estrogen on aneurysmal subarachnoid hemorrhage may find a new drug target to prevent the occurrence of aneurysmal subarachnoid hemorrhage and the secondary injury.

Hyperplastic disease of the breast (HDB) is caused by a hormone imbalance experienced among women at a certain age. Slight breast pain is common in women before menstruation without need of treatment; however, if the pain becomes severe, it can cause physical and mental suffering. Therefore, it is of great clinical significance to control this disease.

Along with the development of society,people pays more attention to health.Since water resources is increasingly missing with depravation,the water quality analysis technique gets a value increasingly,which promotes the development of portable water quality instrument.This paper expounds the present situation and future prospects of portable water quality instrument,puts forward its application in army,aquiculture and enviroment,and enumerates the main technique parameters of several instruments both here and abroad.

Mechanism of diabetes is due to damaged ?-cell or absolute or relative lack of insulin caused by insulin resistance. Stem cells have the potential of proliferation and differentiation into insulin-secreting cells, can also solve the problems of immune rejection. Pancreatic stem cells, embryonic stem cells, bone marrow stem cells and cord blood stem cells can be induced to differentiate into ?-cells, or increased ?-cell regeneration using drugs to play a role in diabetes treatment. Stem cell therapy for diabetes research have made progresses, high blood sugar status have been corrected in some of diabetes animals. But islet development and differentiation mechanisms require deeply studies to gain information for embryonic stem cells induced to differentiate into ? cells. A higher differentiation rate and more mature insulin-secreting cells can be achieved using inducible factor with procedures and application.

This paper aimed to investigate the botanical origins of Isatidis Radix and Isatidis Folium, and clarify the confusion of its classification. The second internal transcribed spacer (ITS2) of ribosomal DNA, the chloroplast matK gene of 22 samples from some major production areas were amplified and sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner. Phylogenetic study was performed using MEGA 4.0 software in accordance with the Kimura 2-Parameter (K2P) model, and the phylogenetic tree was constructed using the neighbor-joining methods. The results showed that the length of ITS2 sequence of the botanical origins of Isatidis Radix and Isatidis Folium was 191 bp. The sequence showed that some samples had several SNP sites, and some samples had heterozygosis sites. In the NJ tree, based on ITS2 sequence, the studied samples were separated into two groups, and one of them was gathered with Isatis tinctoria L. The studied samples also were divided into two groups obviously based on the chloroplast matK gene. In conclusion, our results support that the botanical origins of Isatidis Radix and Isatidis Folium are Isatis indigotica Fortune, and Isatis indigotica and Isatis tinctoria are two distinct species. This study doesn't support the opinion about the combination of these two species in Flora of China.

NDST(New Drug Statistical Treatment)is a powerful tool for treating data of clinical trial and pre-clinical experimentation in the study of new drug. This paper, combined the some examples, introduced the new edition of the software (NDST-21) on the function and characteristics.

Objective To compare the clinical effect of povidone-iedine cream plus vagina scrubbing with traditional preoperative vaginal preparation method in order to seek a more safe,effective,convenient and efficient method to prepare the vagina.Methods 100 cases of married patients undergoing elective gynecological surgery were divided into the observation group and the control group with 50 patients in each.The observation group adopted povidone-iodine cream plus vagina scrubbing and the control group used traditional method.Two groups were prepared in the vagina before and after taking cervical swabs for bacterial culture to compare the positive rate of bacterial culture.Results Intra-group comparison of vaginal bacterial culture before and after vaginal preparation was significantly different,but no statistical significance was seen between the two groups.Conclusions Vagina preparation with povidoneiodine cream plus vagina scrubbing can not only reduce the workload of nurses but also improve patient comfort degree.

Objective To explore the expression of leptin and its receptors in liver tissue of nonalcoholic steatohepatitis(NASH)patient and its significance in pathogenesis.Methods Samples of 59 liver biopsies of NASH patients were divided into three groups G,S and F according to their degree of inflammation and steatosis and stage of fibrosis.The expression of leptin and its receptors including Ob-R,the short form of Ob-R(Ob-Ra)and the long form of Ob-R(Ob-Rb)were semi-quantitatively determined at the protein and/or mRNA expression levels by using immunohistochemistry,in situ hybridization and image analysis.Results The expressions of leptin in NASH liver tissues were found in KC,HSC and MNC,which were mainly situated in zone 3 near steatotic hepatocytes and perisinusoidal or portal fibrosis areas.Leptin mRNA(Ob)positive cells were distributed in hepatocytes in zone 3.The expression of leptin at protein and mRNA levels in the NASH liver tissues was significantly increased in NASH compared with normal liver tissues(P

0.05) and was significantly lower than that in groups of GNB and mycetes' (P