Objecfive To investigate the prevalence of Ureaplasma urealyticum(UU)and Mycoplasma hominis(MH)infections in urogenital tract and their sensitivities to drugs in Qinhuangdao area from 2006 to 2007.Methods UU and MH were detected by Mycoplasma IST kits in urine samples from 3062 patients suspected as urogenital tract infection and the sensitivity tests for 8 antimicrobial agents were performed.Results Mycolasmas were detected in 1262 patients from 3062 patients suspected as urogenital tract infection,in which 1037 were of UU infection,41 were of MH infection and 184 were of UU+MH infection.Both strains were sensitive to josamycin,doxycycline and tetracycline,but were less sensitive to ofloxacin and ciprofloxacin.Conclusions Drug resistance is increasing for Mycoplasma and sensitivity test is important in treatment of urogenital tract infection by Mycoplasma.

0. 05); The long-term effective rate was 84. 21% in the treatment group and 54. 55% in the control group with a significant difference between the two groups (P

Objective To observe the effect of panax notoginseng saponins on pulmonary artery pathology in chronic hypoxic pulmonary hypertensionp (HPH) rats models, discuss the role and possible mechanisms of panax notoginseng saponins in prevention of chronic hypoxic pulmonary hypertension.Methods Forty male Sprague-Dawley (SD) rats were randomly divided into four groups (n =10).Group C: control group;Group H: rats were treated with hypoxia only;Group HC: rats were treated with hypoxia and captopril;and Group HP: rats were treated with hypoxia and panax notoginseng saponins.To observe the effect of panax notoginseng saponins prophylactic treatment in chronic hypoxic pulmonary hypertension rats,after the establishment of the model of hypoxic rat animal pulmonary hypertension models, transthoracic direct pulmonary artery intubation was measured in rat pulmonary arterial systolic pressure and mean pulmonary artery pressure, at the end of separation and cut for pulmonary artery to observe the pathological changes of pulmonary artery, results were recorded and statistically analyzed.Results (1)Transthoracic direct pulmonary artery intubation was measured in rat pulmonary arterial systolic pressure and mean pulmonary artery pressure: pulmonary artery systolic pressure (PASP) and mean pulmonary arterial pressure (MPAP) were significantly higher in Group H than in Group C (P ＜ 0.05), Group HP was no significant difference relative to Group C.(2)Pathological section shew that the primary pathological change of group H is vascular intimal hyperplasia and the proliferation of smooth muscle cells, medial hypertrophy, extracellular matrix increased, thickening of the vessel wall and the vascular stenos.Conclusions Panax notoginseng saponins inhibit the formation of chronic hypoxic pulmonary hypertension and pulmonary vascular remodeling in rats,and have the effect of prevention and treatment of pulmonary hypertension.

Objective To compare the effects of penequinine hydrochloride and atropine on the cardiovascu-lar stability about preoperative in children by monitoring and recording the HR, MAP, SpO2, ECG changes. Methods 40 children underwent selective operation were randomly divided into two groups, 20 in each group. Penequinine hydrechloride group(group A) and atropine group(group B) were injected intramuscularly with penequinine hydro-chloride 0. 015mg/kg or atropine 0. 015mg/kg respectively, at 30 minutes before operation. Patients' HR, MAP, SpO2 and ECG changes were recorded at 10min,20min,30min and 40min after injection. Results The HR,MAP,SpO2 levels of group A and SpO2 level of group B had no significant changes. There are significant changes in HR,MAP of group B. Conclusion Penequinine hydrochloride can be used as preoperative medication and it is conducive to the maintenance of cardiovascular stability.

Confirmation of basic technological parameters of tension levellers is the most important factor of leveling strip. Up to now, most factories have used experts' experience to decide these parameters, without any established rule to follow. For better quality of strip, a valid method is needed to decide technological parameters precisely and reasonably. In this paper, a method is used based on neural network and genetic algorithm. Neural network has a good ability to extract rules from work process of tension levellers. Then using neural network, which has learned from a lot of working samples, to be the evaluation of fitness, genetic algorithm could easily find the best or better technological parameters. At the end of this paper, examinations are given to show the effect of this method.

Objective To investigate the effects on anti-expression of Osteopontin (OPN) by an antisense oligodeoxynucleotide (ASODN) targeting to low free energy region of OPN mRNA and its effects on the proliferation and apoptosis of human breast carcinoma cell line MCF-7. Methods Designing and synthesizing an ASODN based on minimum free energy algorithm in vitro,which targets to the low free energy region of OPN mRNA. Transfecting it into breast carcinoma cell line MCF-7 which expresses OPN in high level. The cell proliferation-inhibitory rate was determined by MTT method;The morphologic change was observed through transmission electron microscope (TEM);The OPN mRNA expression level was checked by RT-PCR method;Cell cycle and apoptosis rate were detected by flow cytometry (FCM) after transfection respectively. Results OPN ASODN inhibited the proliferation of the cells by a time and concentration dependent manner (P

Objective To explore the expression and significance of CD26/DPPⅣand galectin-3 in papillary thyroid carcinoma.Methods Expression of CD26/DPPⅣ or galectin-3 in 68 papillary thyroid carcinomas and 36 thyroid adenomas were determined with EnVision immunohistochemical technique respectively.Results CD26/DPPⅣ and galectin-3 were highly expressed in most of papillary carcinomas but absent or expressed in a low level in most of all thyroid adenomas.With CD26/DPPⅣ as marker to detect papillary carcinoma,the sensitivity,specificity,diagnostic accuracy were 86.8%,97.2% and 90.4%,and with galectin-3 the respective figures were 97.1%,91.7% and 95.2%.There was no statistic significance in intrathyroidal and extrathyroidal invasion,with or without lymph node metastasis,low and poor prognosis groups about positive expression of CD26/DPPⅣ or galectin-3 in papillary carcinomas.Conclusion Both CD26/DPPⅣ and galectin-3 are potential markers of papillary thyroid carcinoma.CD26/DPPⅣ or galectin-3 immunohistochemical staining can be used as a supplement technology for routine pathological diagnosis to differentiate thyroid papillary carcinomas from adenomas,but not accepted as the bio-markers for the invasion,metastasis and prognosis of papillary thyroid carcinomas.

Objective To study the effects and the mechanism of the ethanol extract of Blueberry(BE) on relaxation vascular endothelium or smooth muscle.Methods To use rat aorta as the specimen,to observe the effects of BE on induced relaxation of the phenylephrine-precontracted aorta.and approach the mechanism on vascular endothelium or smooth muscle.Results BE induced relaxation of the phenylephrine-precontracted(1.0×10~(-5)mol·L~(-1) aorta in a dose-dependent way(P<0.01),which was disappeared by removal of functional endothelium(P<0.01).Pretreatment of the aortic tissues with NG-nitro-L-arginine methyl ester(L-NAME),methylene blue,or 1H[1,2,4]-oxadiazole-[4,3_a]-quinoxalin-1-one (ODQ) inhibited the vascular relaxation induced by BE(P<0.01).BE-induced vascular relaxations were also markedly attenuated by addition of verapamil or diltiazem,while the relaxant effect of BE was not blocked by pretreatment with indomethacine,glibenclamide,tetraethylammonium(TEA),atropine,propranolol(P<0.01).Conclusion These results suggest that BE dilates vascular smooth muscle via endothelium-dependent nitricoxide-cGMP signaling pathway,possible involvement of L-type Ca~(2+) channel.

Objective To study the expression of CXCL10 and its receptor CXCR3 expression in bladder neoplasm and ex-plore their clinical significance.Methods Totally 80 patients with bladder neoplasm and 33 healthy control were recruited in this study.The serum level of CXCL10 was determined by ELISA and CXCR3 mRNA in peripheral blood mononuclear cell was meas-ured by RT-PCR.The relationship of serum CXCL10 and CXCR3 levels with clinical parameter were analyzed.Results The serum CXCL10 and CXCR3 levels in patients of bladder neoplasm were significantly higher than the control group(P >0.05).The serum CXCL10 and CXCR3 levels was significantly correlated with lymphatic metastasis (P <0.05).Conclusion High expression of CX-CL10 and CXCR3 may relate to bladder cancer and possibly correlate with lymphatic metastasis.

Objective To investigate the effect of dexamethasone on the toxicity of bupivacaine in murine neurons.Methods Murine neuroblastoma cell line N2a was obtained from ATCC cell bank (USA). The cells were cultured in 10% fetal cow serum/MEM culture medium and divided into 4 groups voup I control (Con); group II bupivacaine ( Bup); group Ⅲ dexamethasone (Dex) and group IV Dex + Bup. The culture medium contained bupivacaine 900 μmol/L in group Bup and dexamethasone 1 μmol/L in group Dex respectively. In group Dex + Bup ( IV ) Bup was added to the culture medium with a final concentration of 900 μmol/L at 12 h after pretreatment with Dex 1 μmol/L. The cells were inoculated in 24 well plates (0.5 ml in each well, 24 wells in each group) and 10 cm culture dishes (7 ml in each dish, 4 dishes in each group). The release rate of LDH was calculated and the morphology of the cells and nucleus condensation (by Hoechst 3334224 fluorescent staining) was detected at 9 h of incubation in 24 well plates. The mitochondrial transmembrane potential (by JC-1 assay) and phosphorylation of Akt and ERKs (by Western blot) were measured at 5 h of incubation in 24 well plates and in culture dishes respectively. ResultsBupivacaine caused severe damage to the N2a cells as evidenced by increase in LDH release and nucleus condensation (apoptosis), dephosphorylation of Akt and ERKs, decrease in mitochondrial transmembrane potential and severe morphological changes. Dexamethasone pretreatment significantly attenuated bupivacaine-induced neurotoxicity. Conclusion Dexamethasone can protect N2a cells from bupivacaine-induced neurotoxicity through stabilization of mitochondrial transmembrane potential and inhibition of dephosphorylation of Akt and ERKs.