Objective To confirm the expression and localization of COX-2 gene in mature rat testis,as COX-2 not only plays a central role in inflammation by up-regulating the synthesis of prostaglandins,but also mediates critical reproductive functions by other mechanisms. Methods In the mature Wistar rats,COX-2 expression and its localization were examined by using immunohistochemistry,RT-PCR and Western-blot. Results The results of RT-PCR and Western-blot showed that COX-2 mRNA and its protein exist in the testis. Expression of COX-2 was localized in spermatogenic cells on immunohistochemical study.No COX-2 expression in sertoli cells and leydig cells was found. Conclusions The present study confirmed the expression of COX-2 in rat testis and its localization in spermatogenic cells.These suggest that COX-2 may play a role in spermatogenesis.

This paper introduces some information of the electronic control system for LGJ-80 freeze dryer, including its system structure, hardware system and software system. The automatic control during overall course, automatic adjustment for degree of vacuum and antijamming are realized in this paper.

Objective To improve the freeze-dryer shelf temperature control effect.Methods The fuzzy-PID control algorithm that's adjusting the PID parameters in real-time in the control process,was adopted to control the shelf temperature,expecting to adapt the changing of process parameters of shelf.Results In the process of freeze-drying temperature control shelves,the shelf temperature static performance and dynamic performance were improved,and the effect of shelf temperature control was archived satisfyingly.Conclusion In the freeze-drying process,the process parameters of shelf are changed along with the drying time and the working environment.The conventional PID control is difficult to obtain satisfactory results.The fuzzy-PID controller,which can adjust PID parameters on real-time in the controlling process,be used in the shelf temperature control,and the result is satisfied.

Objective To investigate the effect of Bushenzhuanggutang combined with salmon calcitonin see calcimar in the treatment of renal osteodystrophy,and explore the mechanism of treatment.Methods 48 patients with renal osteodystrophy were divided into two groups:combined treatment group and control group.The patients were treated with Bushenzhuanggutang and salmon calcitonin see calcimar in combined treatment group.The patients were treated with salmon calcitonin see calcimar in the other group.Then the levels of serum calcium、phosphorus,PTH and BUN、Scr were detected before and after the treatment.Results The level of serum calcium increased significantly in two groups,while the level of serum phosphorus and PTH decreased(P

Objective To explore influence of valsartan on MAU of patients with DN at early stage.Methods 163 patients with DN at early stage were randomly divided into experimental group and control group.Two groups were given routine therapy, and experimental group were added the therapy of valsartan.Period of treatment was 24 weeks, and compared the UAER, Cr, BUN of two groups.Results After treatment of 24 weeks, UAER, Cr, BUN of experimental groups was (142.4 ± 15.6) μg/min, ( 68.7 ±9.4) μmol/L, (3.5 ± 1.2 ) mmol/L, compared with (195.8±23.7)(μg/min,(93.8 ± 13.6) μmol/L, (6.3 ±1.5) mmol/L before treatment, had significant difference (t = 13.675,11.287,2.469,all P<0.05).Compared with( 199.6 ±24.7)μg/min,(87.7 ±11.3)μmol/L、(6.2 ± 1.3)mmol/L of control group after treatment, declined more significantly (t = 13.246,10.312,2.518,all P<0.05).There was no serious complications occurred in two groups.Conclusion Valsartan used in patients with DN at early stage,can significantly improve the function of kidney and enhance clinical effect,decline the side reaction.

OBJECTIVE:To optimize the extraction craft of gentiopicrin from gentian.METHODS:The extraction craft was optimized by orthogonal test with gentiopicrin as an index,and the content of gentiopicroside was determined by TLC scanning method.RESTLUTS:The quantity of menstruum and the extraction times of gentiopicrin had significant influence in the gentiopicrin extraction(P

Objective To develop a high-speed data acquisition system with sampling frequency of 2 GHz to meet the requirement of accurate measurement of flight time.Methods The ALTERA STRATIX Ⅲ series FPGA was used as the main control chip, matching with high-performance ADC chip with sampling frequency up to 2 GHz and sampling accuracy up to 12 bit, while extending 512 MB DDR3 SDRAM for data cache and exchanging data with the host computer through Gigabit Ethernet.In addition, a variety of PCB wiring methods were used to effectively ensure the signal integrity.Results The 250 MHz sine signal was input into the data acquisition board with 2 GHz sampling frequency,by analyzing and calculating the sampling data,acquired the following performance index of SNR=44.6639 and ENOB=7.1.Conclusion This high-speed data acquisition system based on ADC technology is a fast and reliable path and method for the measurement and analysis of biological mass spectrometry data.

Objective To investigate the real type of the first earlier onset spinocerebellar ataxia family in China. Methods Two family members were subjected to autopsy, whose genetypings were confirmed by polymerase chain reaction (PCR) and direct sequencing technique. Golgi staining, immunohistochemistry and electron microscopy methods were used to detect the neurodegeneration in central nervous system of 2 patients. Results The light microscopic and electron microscopic showed synaptic degeneration of Purkinje cell in the cerebellar cortex, which was accompanied by deterioration of Purkinje cell, and both inferior olivary complex, dentate nucleus and anterior central gyrus. Conclusions There is severer neurodegeneration in the central nervous system of earlier onset spiuocerebellar ataxia 6 patient, especially in cerebellar cortex, inferior olivary complex and dentate nucleus, and the neurodegeneration may depend on disease duration.

Objective To observe the oncololigcal and cosmetic outcomes and complication of breastconserving surgery and radiotherapy for early breast cancer. Methods A total of 78 early breast cancer patients, who were treated in the First People's Hospital of Pingdingshan between January 2001 and September 2008, were identified and followed. Among them, 36 were in stage Ⅰ and 42 in stage Ⅱ. Tangential irradiation was used with a dose of 50 Gy. Tumor bed received a boost dose of 10 Gy electron beam. For axillary lymph node-positive patients, ipsilateral supraclavicular field was hybrid irradiated with 50 Gy X and electron beam.Results The total follow-up ranged from 6 to 98 months. Local recurrence was observed among 4.9% of the patients. The 3-year and 5-year overall survival rate were 96.6% and 92.7% ,respectively. The breast cosmetic satisfaction rate was 94.9%. The main complications reported were acute skin reaction and upper limb edema.Conclusion Breast-conserving surgery and post-operative radiotherapy in early breast cancer patients can lead to satisfactory survival rates and cosmetic effects and hence improved life quality.

The objective of the present study was to establish a novel rapid fluorescence focus inhibition test (RFFIT GFP)for the detection of rabies virus antibody, in which a chimeric rabies virus expressing green fluorescent protein (HEP GFP) was used as the basic virus strain in RFFIT GFP assay, and a few serum samples from human, dog and cat were detected by this new method .The optimal serum dilution, virus dosage and infection time were determined in 24 serum samples from human, dog and cat by using RFFIT GFP, RFFIT and ELISA assays. The result showed that these 3 methods gave a good consistency. But RFFIT GFP was found to be more convenient and economic for the detection of rabies virus antibody.