OBJECTIVE： To discuss about the key and entrance points of the development strategy of Chinese materia medica in the new century.METHODS： Some important items,such as difficult problems which Chinese materia medica facing to at present,new way to research into its effective substance bases,innovation on the theory and “ molecular targets” of Chinese materia medica,were analyzed.RESULTS ＆ CONCLUSION： Chinese materia medica industry would become a most important pharmaceutical industry in China and really walk up to the whole world if traditional Chinese herb medicine supplies“ international language” for its exchange,qualitative and quantitative standards on judging the essence of its prescriptions and builds up its“ earmark” of Chinese materia medica industry.Based on the train of thought of this paper,suggestions how to promote the development of Chinese materia medica in the new century are put forward.

Since the first session of Chinese anti-inflammatory immunopharmacological society in 1982,many breakthroughs of immunological research have been made in western countries,such as identification of pathogens by natural immune cells,regulation of immune response,lymphocyte differ?entiation and development. At the same time,much progress has been achieved in anti-inflammatory and immunological research in China,especially in the field of basic scientific issues in immunology, frontier and hot topics,immunological mechanisms of major diseases and related drugs. The course of development of Chinese anti- inflammatory immunopharmacological research has experiences various stages,such as ″follow ″ in the past to ″work together ″ and to ″lead ″ in some research areas in today′s international immunology.

Accurate alignment of knee implants is essential for the success of total knee replacemem.Recently,computer-aided navigation systems have become available,and the results showed more accurate and consistent installation of knee implants.The technology has evolved rapidly with widespread and continue to be developed.The current computer environmem and graphical user interface will become more user friendly and thus gain more widespread acceptance among experienced surgeons With this technology growing and systems evolving,computerized navigation will become the standard of care in knee reconstructive surgery.The key of future studies is to evove less invasive tracker anchoring systems,electronic balancers and computerized saws.A hybrid technique between image-based and image-free methods may develop to produce image-enhanced navigation.It should be an intermediary step between current technology and a fully robotic system.

The cells sense direction is closely related with the proteins that contain PH (pleckstrin homology) domain. PH domain has been found in about 60 proteins, many of which could activate the sequent events of signal transduction via combining with the related binding sites on the surface of chematactic cel ls. The characteristics of this combining are: a.rapid and transient; b.It is only related to the concentration gradient of surroundings outside cells, whi ch is the base of spatial model; c.The distribution of the binding sites on th e cell membrane changes when the researchers altered the position of the chemoat tractant. This is the base of temporal model. To deeply investigate the effects of all kinds of proteins which contain PH domain on cells sense of direction will greatly promote the research of this field, and hence, has great theoretica l significance.

AIM:To study of effects of Panax notoginsengnoside(PnGL) on neutrophil(Neu) functions in carrageenin(Car) induced subcutaneous air-vesicle synovitis in rats and its mechanisms METHODS:Rat air-vesicle synovitis model was established with sc Car(25mg/kg) Neu count,the phagocytosis of Neu,the realease of O2 ?- from Neu and the level of Neu-i were measured with test tube diluting method,staphylococcus aureus phagocytosis method,cytochrome C reduction method and Fura-2 fluorescence technique respectively RESULTS:At 12h after Car used,Neu count of exudates elevated,phagocytosis rate and index of Neu and the release of O2 ?- from Neu increased obviously,suggesting that Neu was in an activated state Mea_nwhile,the level of Neu-i elevated significantly PnGL 60～240mg/kg ip,30min before and 6h after Car sc,reduced Neu count of exudates,inhibited phagocytosis functions and the increase of i level of Neu in a dose-dependent manner There was a significant correlation between the reduction of Neu-i and the inhibition of Neu phagocytosis functions and the release of O2 ?- from Neu Dexamethason had similar effects on Neu functions to PnGL,but with no effect on the level of Neu-i CONCLUSION:The reduction of Neu-i level and the subsequent inhibition of Neu functions is one of the important anti-inflammatory mechanisms of PnGL,which is different from that of Dex

Pancreatic cancer is the fourth most common cause of cancer death,with a 5- year survival rate of less than 5％.The management and prognosis of the patients have remained dismal due to higher resistance of cancer cells to conventional approaches including surgery,radiation and chemotherapy.Therefore,there is a need for development of specific and sensitive tumor marker for pancreatic cancer.Caveolin- 1 is an essential constituent of caveolae and interacts with a variety of cellular proteins and regulates cell- signaling events.In this review,we survey the functional roles of caveolin- 1 in pancreatic cancer and argue that caveolin-1 regulates multiple cancer-associated processes including tumor growth,cell invasion,metastasis,cell apeptosis.However,Caveolin-1 has been reported to impact both positively and negatively on various aspects of pancreatic cancer progression.The function of caveolin- 1 is interdependent on tumor stage and the expression of some signaling pathways that impact on its role during tumor progression.

The process of tumor angiogenesis is a complicated chain reaction which happens under the action of a series of positive feedback and negative feedback factors . Cancer stem cells are small part cell groups of self-renewal and multilineage differentiation potential in tumors. Many studies indicate that tumor stem cells participate in the process of tumor angiogenesis, and vascular endothelial growth factor can regulate endothelial proliferation and angiogenesis, thereby promoting tumor growth and metastasis.

Objective To investigate the effect of hepatitis B virus X protein (HBx) on the induction of eytochrome P450 (CYP) 3A4 by 1α, 25-(OH)2D3 in HepG2 cells in vitro. Methods HepG2 cells were transiently transfected with plasmid pEGFP-N1 (control) or co-transfected with recombinant HBx eukaryotic expression plasmid pcDNA3-X and pEGFP-N1. All HepG2 cells were divided into four groups: control group (without transfection), plasmid pEGFP-N1 transfection group, plasmid pEGFP-N1 transfection plus 1α ,25-(OH)2D3 group, plasmid pcDNA3-X and pEGFP-N1 co-transfection plus 1α ,25-(OH)2D3 group. The expression of CYP3A4 in HepG2 cell was induced by 0.35 μ mol/L 1α ,25-(OH)2D3 for 72 h, and mRNA levels and protein levels of CYP3A4 in the cells were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) assay and Western-blot assay, respectively. The comparison between groups was done by F test. Results CYP3A4 mRNA level in plasmid pcDNA3-X and pEGFP-N1 co-transfection plus 1α ,25-(OH)2D3 group was 1.52 folds of control group, while that in plasmid pEGFP-N1 transfection plus 1α, 25-(OH)2D3 group was 3.97 folds (F= 4.72, P<0. 05). Similarly, intracellular CYP3A4 protein expression in plasmid pcDNA3-X and pEGFP-N1 co-transfection plus 1α , 25-(OH)2D3 group increased to 2.1 folds of control group, while that in plasmid pEGFP-N1 transfection plus 1α,25-(OH)2D3 group increased to 5.9 folds (F=4.68, P<0.05). Conclusion HBx interferes with the induction of CYP3A4 by 1α , 25-(OH)2D3 in HepG2 cell line, which suggests that HBx has suppressive effect on the expression of CYP3A4.

Objective To investigate human esophageal cancer Eca109 cells apoptosis induced by resveratrol.Methods Eca109 cells were treated with difierent concentrations of resveratrol and the change of morphology was observed by inverted microscope and transmission electron microscope in different time;The cell growth inhibitory rate of resveratrol on the proliferation of Eca109 cells were evaluated in vitro by MTT assay.Flow cytometry (FCM)analysis was used to determine the apoptosis of Eca-109 cells.Results Resveratrol was able to inhibit the growth of Eca109 cells,and the suppressive effect was increased with treating concentration and treating time.Resveratrol inhibited the growth of esophageal cancer Eca109 cells in a dose-and time-dependent manner and induced the apoptosis in esophageal cancer.Conclusion Resveratrol could inhibit the proliferation of Eca-109 cells and block the cell cycle at G0/G1.And in the end resveratrol could induce the apoptosis of Eca109 cells.