The present paper studies and analyses the prevalence of epidemic hemorrhagic fever which occured in our university, The results showed that there was direct relationship between epidemic and infection of experimental rats. This paper also discusses the relation among the rat's positive detection rate, the feeding conditions and the rat's age.

Objective To discuss the possibility of transpedicular balloon kyphoplasty for aged osteoporotic thoracolumbar burst fractures.Methods 7 consecutive procedures were performed in 7 patients of aged osteoporotic spinal fractures.The burst fractures were confirmed by CT before the procedure.Although bony fragments were seen to protrud into the spinal canal in the cases,no patient had nerve injury preoperatively.This operation involved the percutaneous insertion of two inflatable bone tamps into a fractured vertebral body transpedicularly under fluoroscopic guidance.Inflation of the bone tamp would elevate the endplates,restoring the vertebral body height,while creating a cavity to be filled with bone cement.Preoperative and postoperative heights were measured.Results 7 patients tolerated the procedure well with immediate relief of their back pain in 24 hours.There was no leakage of cement into the epidural.The height restoration of vertebral was confirmed by x-ray after the procedure.No complications occurred.The patients were allowed to take walk after the procedure.Conclusion Transpedicular balloon kyphoplasty was a effective way to treat aged osteoporotic thoracolumbar burst fractures without obvious canal occupations and neurologic deficit,the procedure can restore height of aged osteoporotic thoracolumbar burst fractures,cement distribution and vertebral canal stenosis can be avoided.

Objective An innovation of operative procedure with biological repair of ligaments for the treatment of acromioclavicular dislocation. Methods Based on the anatomy, physiology, and biomechanics, the operation was designed for restoring the stability of a completely dislocated acromioclavicular joint. Through bone holes drilled along the direction of coracoclavicular ligament fibers, the synthetic absorbable suture was used to rebuild the coracoclavicular ligament for vertical stability of the joint, and then a double loop suture was performed to rebuild acromioclavicular ligament and the capsule for horizontal stability. This biomechanical repair was simple, rapid procedure, and of less tissue trauma. The acromioclavicular joint was steadily fixed without any metallic imbedding, nor interference with joint surface. Results With 1－ 2.5 years follow up, 11 patients recovered with good healing, normal range of shoulder joint motion, and stable fixation. There was no need for secondary procedure to remove the sutures. The curative results of all patients were A degree based on the Karlsson s evaluation. There was no any complication. Conclusion The synthetic absorbable suture could be used with satisfaction for the repair of acromioclavicular dislocation. Compared with routine fixation with metallic material, the new kind of biofixation was not only effective, but also avoided the need of a second procedure for suture removal.

Objective To compare the current trcmment of intertrochanteric fracture fixation commonly used in the two systems:proximal femoral nail(PFN-A)and the dynamic hip screw(DHS)of clinical efficacy and evaluate the treatment of the two intertroehanteric fracture of the gifted bad.Methods The clinical use on closed reduction and traction,respectively PFN-A with DHS and the treatment of intertrochanteric fracture patients with 106 cases and 115 cases,compared with its ease of operation,and the effect of surgery,postoperative complications.Results The clinical material demonstrated that in after PFN-A treatment thighbone thick pmsperom bone fracture technique,technique,the complication is short in DHS.Conclusion PFN-A well-designed,with simple anti-rotation indeed,surgical trauma,less bleeding,fewer complications,etc.,is the treatment of intertrochanteric fracture fixation one of the ideal.

The goats were injected with hepatitis B virus-transfer factor (HBV-TF), which was made from the immune spleen of the same spacies goats immunized with HBsAg After that, we measured the goats cellular immunity and humoral immunity by cheching up the serum anti-HBs level and lymphocyte transformation assay by ~3H-Thymidine (~3H-TdR) incorporation assay. The experiments showed that HBV-TF has the capacity of passing the specific cellular immunity from immune animals to no immune animals. The animals receiving HBV-TF showed a typical skin delay hypersensitivity to specific antigen and augument the effect of lymphocyte transformation in the presence of HBsAg. HBV-TF can also remove the inhibition of lymphocyte transformation caused by HBsAg, Both HBV-TF and N-TF can increase the number of goats blood leucocytes. However HBV-TF and N-TF have no effect on animal's humoral immunity.

IFA was used to observe the distribution and the alteration of T-lymphocyte subtypes in the peripheral blood of 20 patiens with condyloma acuminata for 31 times. The murine antibody was the first antient and the antient of rabbit against IGFITC the second. The results showed that the percentage of CD_3 cell in the patients decreased significantly(P

Objective To study the clinical and neuroimaging features of subtypes of multiple system atrophy (MSA) and their correlations. Methods One hundred and forty-three MSA cases fulfilled Gilman diagnostic criteria (1999) were recruited and their clinical subtypes and stages were classified. Using the staging methods of the pontine cross sign and putaminal slit proposed by Horimoto, 108 patients showed abnormalities in MRI and were further evaluated. The relationship between the subtypes of MSA, disease duration, and MRI abnormalities has been analyzed. Results Of 143 MSA patients, the male-to-female ratio is 1.3:1 ; 93 cases are diagnosed with MSA-C, 39 with MSA-P, and 11 with MSA-P + C; 90 cases with probable diagnosis, and 53 with possible diagnosis. Of the 76 MSA-C cases with MRI abnormalities, 36 (47%) show the pontine cross sign and 10 (13%) show the putaminal slit; of the 24 MSA-P cases with MRI abnormalities, 6 (25%) show the pontine cross sign and 6 (25%) show theputaminal slit. In addition, MSA-C cases with shorter disease duration demonstrate earlier stages of the pontine cross sign. Conclusions In this study, the number of MSA-C cases is more than MSA-P, which might be related to the ethnic background. In neuroimaging, both the pontine cross sign and the putaminal slit are the marked features of MSA. To some degree, the subtypes of MSA are related with the features of imaging, that is, MSA-C patients present the pontine cross sign more often than MSA-P, and the putaminal slit is a comparatively common feature among MSA-P cases.

Objective To apply data mining to risk prediction of sudden deafness, and form the association rules.Methods The clinical data of 517 patients with sudden deafness was collected, including the characteristics of 19 attributes: sex, age, season, hypertension, diabetes, heart disease, hypercholesterolemia, atherosclerosis, long-term smoking, alcoholism, mental tension, insomnia, weakness, bedridden, infection, congenital malformation, trauma, tumour and autoimmune diseases. The source database were cleaned, then mapped for mining database. Minimum support to 0.1 and minimum confidence level to 0.9 were set for analysis of association rules. Results One hundred and six strong association rules were formed, and the rules contained the relation between the incidence of sudden deafness and the characteristics of 19 attributes. Conclusion This method is conducive to make the abstract theory of mathematical statistics into useful association rules to guide the practice of disease prevention and control.

Objective To study the correlation between clinical rating scale and the duration of multiple system atrophy.Methods One hundred and twenty-two MSA cases fulfilling Gilman diagnostic criteria were recruited.Unified Multiple System Atrophy Rating Scale(UMSARS)was applied to assess the degree of the patients' disability.For analyzing the correlation between the UMSARS scores and the duration of the disease,the Spearman's rank correlation coefficients were calculated.Results Among 122 MSA cases,male:female ratio was 1.7:1;disease duration lasted(2.8±1.6)years:possible MSA accounted for 50 cases(41%),probable MSA 72 cases(59%);MSA-P 35 cases(29%);MSA-C 73 cases(60%).MSA-P+C 5 cases(4%),MSA-A 9 cases(7%).The scores of UMSARS,UMSARS-Ⅰ and UMSARS-Ⅱ were found positively correlating with the disease duration.The Spearman's rank correlation coefficients were 0.368,0.266 and 0.392 respectively,all P<0.01;the adjusted Spearman's rank correlation coefficients were 0.360,0.257 and 0.385 respectively.all P<0.01.Conclusions MSA has complex clinical manifestations.which should include MSA-P+C subtype.UMSARS is a reliable scale to mirror the progression of MSA.which is useful to study the clinical features and disease duration of MSA.

Aim To study the effects of ferulic acid (FA) on E-selectin expression in human umbilical vein endothelial cells (HUVECs) activated by lipopolysaccharide and leukocyte-endothelial cell adhesion. Methods The effects of FA on E-selectin and E-selectin mRNA expression were determined by flow cytometry and reverse transcription polymerase chain reaction. The effect of FA on HL60-HUVEC adhesion was evaluated with the method of staining the cells by Rose Bengal. Results The expression of tively). Conclusion FA can inhibit the expression of E-selectin and E-selectin mRNA and HL60-HUVEC adhesion. This may contribute to its protective effect against ischemia-reperfusion injury.