Chronic myeloid leukemia (CML) is one of myeloid malignancies,and there is no effective treatment method at present.Nowaday,many studies report that combined treatment and adoptive immunotherapy can rebuild immunological function of patients,it's an ideal method of cure CML.So searching optimal leukemia-associated antigens and researching the number of antigen specific T lymphocytes is the key to treat CML.

AIM:To investigate the mechanisms of Myospalax fontanieri bone extracts for macrophages activation. METHODS: Complete Freund's adjuvant was used to induce mouse celiac macrophage activation by ip,then peritoneal exudate cells were cultured in vitro in the present of LPS and IFN-?,concentrations of IL-1?,IL-6,IL-12p40,and TNF-? were by ELISA measurement and the MAC-1 cell proportion was determined by FACS Calibur. RESULTS: Myospalax fontanieri bone had immunosuppressive effect on mouse celiac macrophages,and markedly decreased production of inflammatory Cytokine. CONCLUSION: Inhibition of inflammatory factor may be the mechanism of action for rheumatoid arthritis.

Objective To discuss the possibility of transpedicular balloon kyphoplasty for aged osteoporotic thoracolumbar burst fractures.Methods 7 consecutive procedures were performed in 7 patients of aged osteoporotic spinal fractures.The burst fractures were confirmed by CT before the procedure.Although bony fragments were seen to protrud into the spinal canal in the cases,no patient had nerve injury preoperatively.This operation involved the percutaneous insertion of two inflatable bone tamps into a fractured vertebral body transpedicularly under fluoroscopic guidance.Inflation of the bone tamp would elevate the endplates,restoring the vertebral body height,while creating a cavity to be filled with bone cement.Preoperative and postoperative heights were measured.Results 7 patients tolerated the procedure well with immediate relief of their back pain in 24 hours.There was no leakage of cement into the epidural.The height restoration of vertebral was confirmed by x-ray after the procedure.No complications occurred.The patients were allowed to take walk after the procedure.Conclusion Transpedicular balloon kyphoplasty was a effective way to treat aged osteoporotic thoracolumbar burst fractures without obvious canal occupations and neurologic deficit,the procedure can restore height of aged osteoporotic thoracolumbar burst fractures,cement distribution and vertebral canal stenosis can be avoided.

Accurate alignment of knee implants is essential for the success of total knee replacemem.Recently,computer-aided navigation systems have become available,and the results showed more accurate and consistent installation of knee implants.The technology has evolved rapidly with widespread and continue to be developed.The current computer environmem and graphical user interface will become more user friendly and thus gain more widespread acceptance among experienced surgeons With this technology growing and systems evolving,computerized navigation will become the standard of care in knee reconstructive surgery.The key of future studies is to evove less invasive tracker anchoring systems,electronic balancers and computerized saws.A hybrid technique between image-based and image-free methods may develop to produce image-enhanced navigation.It should be an intermediary step between current technology and a fully robotic system.

OBJECTIVE： To discuss about the key and entrance points of the development strategy of Chinese materia medica in the new century.METHODS： Some important items,such as difficult problems which Chinese materia medica facing to at present,new way to research into its effective substance bases,innovation on the theory and “ molecular targets” of Chinese materia medica,were analyzed.RESULTS ＆ CONCLUSION： Chinese materia medica industry would become a most important pharmaceutical industry in China and really walk up to the whole world if traditional Chinese herb medicine supplies“ international language” for its exchange,qualitative and quantitative standards on judging the essence of its prescriptions and builds up its“ earmark” of Chinese materia medica industry.Based on the train of thought of this paper,suggestions how to promote the development of Chinese materia medica in the new century are put forward.

Objective To study the applied value of invasive arterial blood pressure monitoring in the diagnose and treatment of coronary artery intervention. Methods The dynamic changes of ECG, the non-invasive blood pressure, the invasive arterial blood pressure monitoring and the clinical performance were ob-served in 163 patients in the process of coronary artery intervention (CAI). Then the invasive arterial blood pressure change and the non-invasive blood pressure change were compared. Results The change rates of invasive arterial blood pressure were higher than that of non-invasive arterial blood pressure. The difference of both changes had statistical significance. The invasive blood pressure decreased and wave forms changed in 26 cases. These reflected sensitively the patients' condition and the issues in the process of CAI. Conclusions To strengthen the invasive arterial blood pressure monitoring can improve the success rate of CAI more effec-tively and reduce the complication.

The failure of islet β cell is the core pathogenic mechanism of diabetes,which is also the key target for type 2 diabetic mellitus treatment.A large amount of basic and clinical researches have been confirmed that glucagon like peptide-1 and its analogue liraglutide have abilities to significantly improve islet β cell function besides the glucose-dependent lowering hyperglycemia effects.Furthermore,liraglutide is approved to improve β cell function,thus providing a new choice for treatment of type 2 diabetes.

Asthma is a extensive airway limited disease caused by allergens or other factors and emerging under bronchial hyper-responsiveness,which is a common chronic respiratory disease.Airway inflammation and airway remodeling is the main feature of bronchial asthma,and airway remodeling is the main pathological basis causing the irreversible airflow obstruction and pulmonary function injury of asthma.The main pathological change of airway remodeling is airway smooth muscle layer thickening induced by airway smooth muscle cell (ASMC) proliferation,and the mast and increasing number of ASMC under the action of various cytokines is the main cause of smooth muscle layer thickening.The function of airway smooth muscle cells in the airway remodeling is described.

Objective To evaluate the efficiency of entecavir in chronic hepatitis B (CHB)patients.Meathods 60 cases of pa-tients treated with entecavir 0.5 mg/d.Observed the negative rate of HBV DNA and ALT after 3,6 and 12 months.Used immuno chemiluminescence to observe the HBeAg titer after medication.Results The negative rate of ALT and HBeAg, HBeAg conversion rate were 91.7%,86.7% and 16.7%,respectively.The titer of HBeAg was low at baseline,the patients would had higher HBeAg seroconversion,there were significant differences with other groups.The titer of HBeAg decreased≥90% patients was significantly higher in patients with < 90% on the 3 months.Conclusion The HBeAg titer could be predicted ETV for treatment of HBeAg positive CHB patients.

Danggui Injection and Vitamin B12 Injection were injected into acupoints Geshu (BL17), Pishu (BL 20), Zusanli (ST 36), Fenglong (ST 40) and Neiguan (PC 6) to treated 56 cases of hyperlipidemia. Meanwhile, Fenofibrate was adopted to treat another 45 cases as control. After2-month treatment, result showed similar effects in the two groups.