Objective To research the clinical effect of complex trabeculectomy in the treatment of glaucoma. Methods 80 patients with glaucoma were selected in the study.They were randomly divided into two groups. 40 patients in the control group were given traditional trabeculectomy technique,and 40 patients in the treatment group were given composite trabeculectomy.The incidence of shallow anterior chamber,intraocular pressure,one year after the formation of a functional follicles were analyzed and compared in the two groups.Results The incidence rate of shallow anterior chamber in the treatment group (6.98%)was lower than the control group(23.81%).6 months, 12 months after operation,the IOP of the treatment group was lower than the control group,and less than 21mmHg. After 1 year,the formation rate of functional follicles of the treatment group (90.70%)was higher than the control group (71.43%),the difference between the two groups was significant(χ2 =5.16,P <0.05).Conclusion The treatment effect of composite trabeculectomy for glaucoma is significant.

Tubulointerstitial fibrosis is the final common pathway of most progressive renal diseases, and also is the pathological basis that leading to chronic renal failure. In recent years, the biological effect of tissue transglutaminase in the progressive tubulointerstitium fibrosis attracts more and more attention. Tissue transglutaminase belongs to a group of calcium-dependent mammalian enzymes that have the capacity to irreversibly cross-link proteins through the formation of e (γ-glutamyl)lysine bonds, and lead to the excessive deposition of extracellular matrix, then functions markedly in the tubulointerstitial fibrosis.

OBJECTIVE:To investigate the utilization of aztreonam in perioperative patients in order to promote rational use of antimicrobial agents. METHODS: 215 discharged surgery cases were collected in Mar. 2009 and analyzed retrospectively to evaluate the rationality of drug use. RESULTS: Of 215 cases, preventive use accounted for 122 cases, among which rational use 29 cases and irrational use 93 cases; treatment use accounted for 93 cases, among which reasonable use 54 cases and unreasonable use 39 cases. CONCLUSION: Perioperative use of aztreonam was not in line with Guiding Principles for Clinical Use of Antibacterial Drugs, Notice for Further Strengthen Management of Clinical Use of Antibacterial Drugs Issued by General Office of the Ministry of Health. Unreasonable utilization require to arouse attention of medical personnel and strengthen management.

Unfolded protein response(UPR) is a protective response in cell endoplasmic reticulum(ER) under stress condition.Three ER transmembrane proteins,IRE1,PERK,and ATF6,coordinately regulate the UPR function in mammalian cells through their signaling pathways.In addition,some proteins and transcription factors during the UPR can provide negative and positive feedback loops to maintain the normal function of ER.UPR can trigger cell death or apoptosis and eventually cause related diseases if the ER stress persists.Several key mediators of UPR are candidates for therapeutic targets in many studies.Up to now progress has been made in the area,which provides new ideas for clinical practice and holds a great potential for future application.

To investigate the relationship between esophageal motility and GI hormones in patients suffering from gastroesophageal reflux disease (GERD), the esophageal motility in 20 GERD patients with esophagitis and 25 GERD patients without esophagitis was examined by means of SGY-3 Digestive Tract Motility Measuring Instrument. Meanwhile,Serum gastrin,motilin,glucagon,estradiol were measured with RIA.The data from 15 healthy volunteers served as control.The results showed that ①GERD patients without esophagitis had lower LESP(2 12?1 00kPa) than control(3 23?0 72kPa P

Objective To determine the endogenous metabolites in blood of rats with Xiaoqinglong Decoction Syndrome by using GC-MS;To provide evidence for researches on the essence of Xiaoqinglong Decoction Syndrome. Methods Totally 36 male Wistar rats were randomly divided into blank control group (10 rats), model group (13 rats), and Xiaoqinglong Decoction group (13 rats). Model group and Xiaoqinglong Decoction group were used to make the models of exogenous cold and internal fluid syndrome (Xiaoqinglong Decoction Syndrome). Rats in Xiaoqinglong Decoction group received gavage with Xiaoqinglong Decoction, while rats in blank control group received nothing. Blood of rats in the three groups was taken from the abdominal aorta and detected by GC-MS 15 days later, and analyzed by multivariate statistical analysis with PLS-DA. Results 13 kinds of biomarkers were found:lactic acid, 3-hydroxy-butyric acid, glycine, serine, lysine, dextrose, arachidonic acid and other metabolites. Conclusion Expressions of biomarkers, such as lactic acid and glycine, are energy metabolism, immune function, and inflammation, which provide material basis for researches on the essence of Xiaoqinglong Decoction Syndrome.

Objective To investigate the relationship between the pathogenesis of leukemia and Flt3 gene by detecting the point mutations in juxtamembrane domain coding by Flt3. Methods The exons 14, 15 of Flt3 gene were detected using PCR, PCR/SSCP and gene sequence in 60 leukemia patients with peripheral blood and bone marrow samples. Results We found Flt3-L576P point mutation in 3 cases in juxtamembrane domain in 60 patients, including 1 patient had a deletion of intron 14. Conclusion Flt3-L576 was a new point mutation site, which way be associated with the pathogenesis and development of leukemia.

We described clinical process of two cases of intraocular lymphoma in aspects of early diagnosis by fine needle aspiration (FNA) and biopsy and treatment by intravitreal methotrexate (MTX). Two patients were suspected to have primary intraocular lymphoma (PIOL) with geographic yellow-white infiltrates and vitreous opacity. FNA confirmed malignant intraocular lymphoma in one patient and failed in the other patient due to complication of vitreous hemorrhage. Subsequent vitreous biopsy confirmed malignant intraocular lymphoma in the other patient. Both patients were treated by intravitreal methotrexate. In case 1 the tumor had complete remission and follow-up of 12 months had not found any signs of recurrence. In case 2 the patient died of brain metastasis 22 months after the ocular biopsy. Our findings demonstrate that although cytological examination of vitrectomy specimens remains the gold standard in diagnosis of PIOL, examination of FNA and biopsy increases the reliability of early diagnosing or excluding a PIOL. Individualized intravitreal methotrexate can be used to effectively treat PIOL. More effective integrated program treating primary central nervous system lymphoma/PIOL is worthy of looking forward to.

Objective: To optimize the extraction process of chlorogenic acid in flos lonicerae.Methods: The flos lonicerae was treated by means of cellulase and/or pectolase before extracted by ethanol reflux, studying the enzyme dosage, treatment time, treatment temperature how to influence the percentage of extraction of flos lonicerae and the amount of chlorogenic acid in extrction.Results: The percentage of extraction of flos lonicerae and the amount of chlorogenic acid in extrction could be obviously increased by the cellulase treatment, 8.15g chlorogenic acid could be extracted from 100g flos lonicerae. The optimum temperature of treatment was 40℃～50℃; The percentage of extraction was higher by the combined treatment of cellulase and pectilase than by cellulase only, but the amount of chlogenic acid was not obviously different between these two methods.Conclusion: The amount of chlorogenic acid in extrction could be increased by enzymic treatment.