1.Influence of granulocyte colony-stimulating factor on dendritic cells in donor of allogeneic hematopoietic stem cell grafts
Journal of Leukemia & Lymphoma 2011;20(7):415-417
Objective To evaluate the influence of granulocyte colony-stimulating factor (G-CSF) on the quantity and ratio of type 1 dendritic cells (DC1) and type 2 dendritic cells (DC2) in normal allogeneic hematopoietic stem cell graft donor peripheral blood (PB) and bone marrow (BM). Methods G-CSF treatment with 10 μg/kg per day for 5 days, the quantity, ratio of DC1 and DC2 in G-CSF primed allogeneic peripheral blood stem cell graft (G-PBSC) were detected in 11 donors, 20 donors in G-CSF primed allogeneic bone marrow stem cell graft (G-BMSC), 8 donors in unprimed PB, and 10 normal individuals in unprimed BM,respectively. by flow cytometry method. Results After treated by G-CSF, the mean BMDC2 was increased from 14.37×106/L to 29.68×106/L ( t = 2.433, P = 0.022) in control group, whereas BMDC1 count did not change (13.77×106/L vs 18.88×106L, t = 1.169, P = 0.251). The ratio of DC2 to DC1 was significantly higher in G-CSF treated donor BM (1.83±0.81 vs 1.12±0.32, t = 2.658, P =0.013). After G-CSF treatment of normal donor, the number of PBDC2 (14.92×106/L vs 26.76×106/L, t = 2.390, P = 0.029), and the ratio of DC2 to DC1 was increased (1.00±0.37 vs 2.02±1.43, t = 2.158, P = 0.044), but the number of PBDC1 was similar (14.21×106/L vs 18.02×106/L, t = 0.625, P=0.541). Conclusion G-CSF treatment of normal allogeneic HSC donor selectively increase the number of PBDC2 and BMDC2, but do not change PBDC1 and BMDC1.
2.Possible Ethical Problems about the Application of Mitochondrial Replacement Technique on Human Reproduction
Chinese Medical Ethics 2016;29(2):268-270
Applying mitochondrial replacement technique to human assisted reproduction could benefit the fam-ilies with mitochondrial diseases and improve clinical outcomes of assisted reproduction in elderly patients. But it may also lead to a series of medical ethics issues such as the standardization and security of this technology, the conflict with the traditional ethical concepts, the determination of informed consent and confidentiality principles, the health development issues of the offspring, and commercial donation of eggs and so on. As for this, the relevant institutions should strictly control the indications when apply this technology, the government should formulate rele-vant laws and regulations. Simultaneously, it is important to strengthen supervision and management of social eth-ics, improve professional ethics of assisted reproductive personnel, and emphasize the correct guiding role of public opinion.
3.Research progress of CD4+ CD25+ regulatory T cells in blood diseases
Journal of Leukemia & Lymphoma 2014;23(4):250-252
CD4 + CD25 + regulatory T cells are produced in the thymus and have anergic and immunosuppressive properties.Researchers measured the antibody level related with CD4+ CD25+ T cells,CD4+ CD25hi T cells,CD4+ CD25+ Foxp3 and CD4+ CD127 in regenerative anemia,leukemia,ITP,NHL,MDS.Then they got the conclusions that regulatory T cells decreased in the autoimmune blood diseases and increased in the neoplastic diseases.The regulatory T cells have great prospect in the treatment of autoimmune diseases,allergic diseases,transplant rejection,tumors and specific vaccination.
4.Interobserver variation in the measurement of patellar height after total knee arthroplasty
Jiuyi SUN ; Xiaohua LI ; Qingyun XIE
Orthopedic Journal of China 2006;0(03):-
[Objeetive] To assess the reproducibility and accuracy of four ratios used to measure patellar height,named the Blackburne-Peel,Caton Deschamps,Insall-Salvati and modified Insall-Salvati,before and after totalknee arthroplasty.[Methods]The patellar height was measured,by means of the four ratios,on the pre-and post-operative lateral radiographs of 44 patients(45 knees)who had undergone total knee arthroplasty.Two independent observers measured the films sequentially,in identical conditions,totally 720 measurements per observer.[Results]Before operation there was greater interobserver variation using either the Insall-Salvati or modified Insall-Salvati ratios than that when using the Caton-Deschamps or Blackburne-Peel methods.This was due to difficulty in identifying the insertion of the patellar tendon.Before operation,there was a minimal difference in reliability between these methods.After operation the interobserver difference was greatly reduced using both the Caton-Deschamps and Blackburne-Peel methods,which used the prosthetic joint line,compared with the Insall-Salvati and modified Insall-Salvati,which refered to the insertion of the patellar tendon.[Conclusion]The theoretical advantage of using the Insall-Salvati and modified Insall-Salvati ratios in measuring true patellar height after total knee arthroplasty needs to be balanced against their significant interobserver variability and inferior reliability when compared with other ratios.
5.?_2-AR gene cloning from human detrusor cell and the construction of its antisense eukaryotic expression vector
Gang LI ; Changcheng SUN ; Xiaohua ZHENG
Chinese Journal of Urology 2001;0(06):-
Objective To clone human beta-2 adrenoceptor gene from human bladder smooth muscle and to construct its antisense eukaryotic expression vector. Methods The ?_2-AR full length cDNA was cloned from human detrusor cells through RT-PCR and subcloned into clone vector (pUC18).The objective gene was then cut from ClaⅠ/HindⅢ sites of pUC18 with restriction endonulcease and subcloned into pLNCX vector in trans-direction.Finally the constructed ?_2-AR gene antisense expresstion vector was identified through restriction endonuclease analysis and sequencing. Results The sequence of cloned ?_2-AR full length cDNA was certified by comparison with the database of the Genebank.The constructed antisense eukaryotic expression vector was proved to be same with designed by restriction endonuclease analysis and sequencing. Conclusions ?_2-AR full length cDNA was cloned and its antisense eukaryotic expression vector was successfully constructed.This technique establishs the foundation for the further research on drug treatment of bladder dysfunction.
6.RELATIONSHIP BETWEEN ESOPHAGEAL MOTILITY AND SERUM GASTROINTESTINAL HORMONES AND ESTRADIOL IN PATIENTS WITH GASTROESOPHAGEAL REFLUX DISEASE
Kabing ZHAO ; Xiaohua SUN ; Rongbi GUO
Medical Journal of Chinese People's Liberation Army 2001;0(12):-
To investigate the relationship between esophageal motility and GI hormones in patients suffering from gastroesophageal reflux disease (GERD), the esophageal motility in 20 GERD patients with esophagitis and 25 GERD patients without esophagitis was examined by means of SGY-3 Digestive Tract Motility Measuring Instrument. Meanwhile,Serum gastrin,motilin,glucagon,estradiol were measured with RIA.The data from 15 healthy volunteers served as control.The results showed that ①GERD patients without esophagitis had lower LESP(2 12?1 00kPa) than control(3 23?0 72kPa P
7.Study on high-throughput respiratory tract virus detection method based SPR
Qiuxiang SUN ; Xiaohua WANG ; Dayong GU
International Journal of Laboratory Medicine 2016;37(15):2068-2070,2073
Objective To develop a biosensor method with strong specificity and high‐throughput by combining with the surface plasmon resonance (SPR) and gene chip technique and aiming at 9 kinds of common respiratory tract viruses including influenza A and influenza B ,(Influ A ,B) ,H1N1 ,respiratory syncytial virus (RSV) ,parainfluenza virus 1 -3 (PIV1 -3) ,adenovirus (ADV) and coronavirus (SARS) leading to severe acute respiratory syndrome .Methods Firstly the software primer 5 was used to design the specific primer and probe of related viruses in the conserved sequence ;the designed nine kinds of corresponding respiratory virus probes were immobilized in the specific region of SPR chip after chemical modification .The SPR technique was applied to conduct the real time monitoring the hybridization process of the probe with the PCR products .Finally the signal amplification was realized by the biotin and streptavidin system .Results The designed gene chip could detect 9 kinds of respiratory tract viruses by high‐throughput with better detection specificity ;the chip surface could be reutilized after certain regeneration condition ,which avoided the influence of intra‐batch difference on the results ;the detection sensitivity reached the nanomole level .Conclusion The prelimi‐nary study results demonstrate that using the SPR biosensor technique to establish a high‐‐throughput detection of respiratory tract viruses has some practicability and feasibility ,and is expected to become a rapid ,large scale and high‐ throughput measure for screening respiratory tract viruses with good application prospect .
8.CT findings of primary pulmonary non-Hodgkin lymphoma
Gang PENG ; Xiaohua ZHU ; Xiwen SUN ; Sen JIANG ; Jun MA
Chinese Journal of Radiology 2008;42(2):141-144
ObjectiveTo analyze the CT appearances of primary pulmonary non-Hodgkin lymphoma(PPNHL)in order to improve its diagnosis.MethodsCT manifestations of 19 cases with PPNHL confirmed by pathology and clinical follow-up were retrospectively analyzed.ResuitsNodules 7 cases and masses 8 cases in PPNHL were ill-defined and markedly enhanced,in which air bronchogram was commonly seen.Muhiple patchy areas distributed in the bilateral lungs were found in 9 cases.Consolidation 7 cases was lobar and markedly enhanced,in which air bronchogram was commonly seen.Interstitial change manifested as bilateral diffuse ground-glass opacities and reticular shadow was found in 1 case.Mixed imaging manifestations were detected in 10 cases.Pleural effusion 4 cases was uncommon.Conclusion CT manifestations of PPNHL are varied,but some specific imaging features still exist,CT examination combined with clinical manifestations is helpful for the diagnosis of PPNHL.
9.Clinical efficacy of percutaneous vertebroplasty in the treatment of osteoporosis vertebral compression fractures
Zhiyong ZHANG ; Xiaowei SUN ; Guangyue XIE ; Xiaohua HOU
Chinese Journal of Geriatrics 2013;(4):419-422
Objective To compare the clinical efficacy and safety between percutaneous vertebroplasty (PVP) and conservative treatment for osteoporosis vertebral compression fracture (OVCF).Methods A total of 55 patients undergoing PVP and 65 patients with conservative treatment for OVCF in our hospital from 2006 to 2011 were retrospectively analysed.The degree of pain and personal life before treatment and after treatment at different time points were investigated by visual analogue scale (VAS) pain scale and Oswestry Disability Index (ODD.Results The degree of pain in lower back was reduced after the treatment.The VAS pain scales in PVP group versus control group were (8.3±0.5) vs.(8.50.6),(6.9±0.7) vs.(4.5±0.7),(5.40.6) vs.(3.4±0.5),(4.8±0.3) vs.(2.8±0.5),(4.0±0.4) vs.(2.3±0.4),(3.1±0.5) vs.(2.0±0.2)before treatment and 1 week,1,3,6 and 12 months after treatment,respectively.The VAS pain scales were reduced more rapidly in PVP group than in control group,showing a better relief of pain.There were differences in VAS pain scales between the two groups at different time points (t=17.89,19.74,28.38,24.49,14.01,respectively,all P<0.01).The ODI scores in PVP group and control group were [(88.8±5.0) vs.(87.2±5.1)],[(72.6±7.8) vs.(53.1±6.3)],[(62.0±8.5) vs.(37.2±6.0)],[(47.1±12.6) vs.(26.5±3.6)],[(36.7±9.3) vs.(17.9±5.5)],[(24.3±10.0) vs.(9.4±2.5)] before treatment and 1 week,1,3,6 and 12 months after treatment,respectively.The ODI scores were reduced more rapidly in PVP group than in control group,showing a better recovery of daily life.There were differences in ODI scores between the two groups at different time points (t=15.00,18.17,11.74,13.20,10.72,respectively,all P<0.01).Conclusions PVP can alleviate pain and restore daily life rapidly.It shows advantages in rccovery of the fractured vertebrae height.
10.Clinical study of low dose heparins and low molecular weight heparins for treatment of traumatic disseminated intravascular coagulation
Junmin WEN ; Huaisheng CHEN ; Yuxin SUN ; Xiaohua PAN
Chinese Journal of Trauma 2012;28(8):736-740
ObjectiveTo discuss the clinical efficacy of low dose heparins and low molecular weight heparins (LMWHs) in the treatment of severe traumatic disseminated intravascular coagulation (DIC).MethodsA total of 77 trauma patients (APACHE Ⅱ score for 5-10) with secondary DIC in ICU were included and randomly assigned to three groups,ie,Group A (26 patients,subcutaneously injected with LMWHs at doses of 75-150 U · kg-1 · d-1,with the average incipient dose of 4 000 U/d),Group B (25 patients,subcutaneously injected with heparins at doses of 100-250 U · kg-1 · d-1,with the average incipient dose of 5000 U/d and control group (26 patients,supplemented with simple blood coagulation factor).The injection volume was adjusted according to the level of antithrombin Ⅲ ( ATⅢ ) in each group.Within 28 days in ICU,the three groups were compared in aspects of deaths,hospital day,bleeding rate,thrombin time (TT),prothrombin time (PT),activation part thrombin time ( APTT),fibrinogen (Fg),antithrombin Ⅲ ( AT Ⅲ ) and D-dimer.Results ( 1 ) The differences of mortality were both insignificant in the control group comparel with Group A and Group B respectively at day 28 ( P < 0.05 ).(2) The ICU stay of three groups showed significant differences ( P < 0.01 ),with longer stay of Groups A and B than control group ( P < 0.01 ) and longer stay of Group A than Group B ( P < 0.05 ).( 3 ) Except for AT Ⅲ ( P < 0.01 ),other blood coagulation indices showed no significant difference among three groups.(4) The differences of bleeding rate were not significant between Group A and control group (P > 0.05),but significant between Group B and control group (p < 0.05 ).(5) Related factor analysis indicated significant relationship between AT Ⅲ level and bleeding rate (P <0.01 ).ConclusionsLow dose heparins and LMWHs are beneficial to controlling traumatic DIC,but the heparin therapy adjusted by the level of AT Ⅲ with marked correlation bleeding rate shows lower bleeding rate as compared with LMWHs.