Objective The F10 gene was found in the initial stage of our study to be highly expressed in hydatidiform mole. The aim of this study was to investigate the effects of theoverexpression or depletion of F10 on the invasiveness of the choriocarcinoma cell line JAR and explore the relationship of F10 expression with the invasiveness and metastasis ofchoriocarcinoma. Methods Using cell transfection and RNA interference technology, we established JAR choriocarcinoma cell lines with stablyoverexpressedor silenced F10 gene.We randomly and equally assigned 30 SPF nude mice into the three groups to receive the injection of JAR cellswith overex-pressed F10 ( F10 overexpression group) , untreated JAR cells ( control group) , and JAR cells with silenced F10( F10 silence group) . At 5 weeks after the JAR cell injection, we harvested the subcutaneous tumor tissues from the mice, determined the expressions of ma-trix metalloproteinases (MMP), tissue inhibitors of metalloproteinase-1(TIMP-1), and plasminogen activator inhibitor-1(PAI-1), and compared by Western blot and immunohistochemistry, and compared the expressions among the three groups of mice. Results Im-munohistochemistryshowed significantlyup-regulated expressions of MMP-2, -8, -11, -16, and-19 and down-regulated expressions of TIMP-1 and PAI-1 in the subcutaneous tumor tissues in the F10 overexpression group as compared with the control and F10 silence groups ( P<0.05) .Western blot also exhibitedextremely significantly up-regulated expressions of MMP-2,-8,-11,-16, and -19 pro-teins anddown-regulated expressions of TIMP-1 and PAI-1 proteins in the F10 overexpression groupin comparison with the control and F10 silence groups( P<0.01 ) . Conclusion By up-regulating the expressions of MMPs and down-regulating the expressions of TIMP-1 and PAI-1, the F10 gene might be an upstream stimulating factor involved in the proliferation, invasiveness, and metastasis of-choriocarcinoma cells.

The role of DS-182 in protection of cellular damages induced by oxygen free radicals was studied in rat cardiac mitochondria. The changes of cardiac mitochondrial enzyme (cytochrome oxidase) and P/O, RCR were used as indices of mitohondrial injury. The experimental animals were divided into three groups: control group, injury group (Fe~(2+)+ascorbic acid), and protection group (DS-182+Fe~(2+)+ascobic acid). Results obtained showed that incubation of mitochondrial suspension with ascorbic acid and Fe~(2+) resulted in decrease of activity of cytochrome oxidase, P/O (with ?-ketoglutarate andsuccinate as substrate respectively) and RCR, which indicated that the structure and function of mitochondria were damaged. Danshensu (DS-182) can inhibit the decrease of cytochrome oxidase activity, P/O and RCR. Thus, DS-182 with acts as an effective scavenger for oxygen free radicals prevented the mitochondrial injury.

Supplementary concept records ( SCB ) can effectively supplement and expand MeSH by forming the mapping between SCB and MeSH. Chinese herbal medicine terms indentified by MeSH Browser in SCB were analyzed in aspects of their distribution,single medicine,abstracted compositions,compound prescription,classical prescription, slef-made prescription, and use frequency,which showed the supplementary concepts in relation with Chinese herbal medicine and the existed problems. Suggestions were put forward for the use of Chinese herbal medicine terms in MeSH SCB, such as promoting their standardization, and use of standard terms when scientists publish their papers and retrieve papers from databases.

AIM: To investigate the contribution of angiotensin-converting enzyme inhibitor (ACEI) to the regulation of calpain system in infarcted myocardium. METHODS: Rat myocardial infarction (MI) model was established by permanent ligation of the left coronary artery. The treatment with the ACEI inhibitor rampril (1 mg?kg~-1 ?d~-1 ) was started 7 days prior to surgery. On day 1, 3, 7 and 14 after MI, protein levels of calpainⅠ, Ⅱ and calpastatin were determined in left ventricular free wall (LVFW), interventricular septum (IS) and right ventricule. RESULTS: CalpainⅠprotein level was increased in IS 14 d post MI, whereas the protein level of calpainⅡ was maximally increased in LVFW 3 d post MI. Rampril decreased protein up-regulation of calpainⅠ and Ⅱ, and reduced infarct size and interstitial fibrosis. Calpastatin protein expression was not affected by ACEI. CONCLUSIONS: CalpainⅠ is involved in cardiac remodelling in the late and calpainⅡ contributes to cardiac tissue damage in the early phase of MI. The heart protective effect of ACEI may be related to the inhibition of calpain system in the pathogenesis of myocardial infarction.

By the needle-warming moxibustion, 43 cases of diabetic vesical pathological changes,together with 43 cases in the control group, were treated for 30 days, to observe the times of urination,volume of urine and residual urine inside the bladder before and after treatment every day. It has been indicated in observation that needle-warming acupuncture therapy can decrease the time of urination every day, elevate the urine volume each time and decrease the residual urine in the bladder, with a significant difference (P＞0.05) in comparison with before the treatment.

Purpose To determine the effect of RNase MC2 purified from momordica charantia on cell growth of hepatocellular carcino-ma ( HCC) and its underlying mechanism. Methods MTT, colony formation and nude mice model were used to examine the activity of RNase MC2 in cell proliferation. Cell cycle analysis was done by flow cytometry. Autophagy induced by RNase MC2 treatment was observed via transmission electron microscope. Western blot was performed to detect the RNase MC2-mediated changes of proteins. Re-sults In vitro and in vivo data showed that RNase MC2 markedly inhibited HCC cell proliferation, arrested cells at G2/M phase by in-creasing expression of p53 and p21, induced autophagy via upregulating Beclin-1 and LC3-Ⅱ. Furthermore, combination of RNase MC2 and Sorafenib exerted enhanced lethal effect on HCC cells. Conclusion RNase MC2 manifests significant antitumor activities and enhances the killing effect of Sorafenib in HCC via inducing cell cycle arrest and autophagy.

OBJECTIVE To investigate the clinical curative effect of acellular dermal matrix contrast with temporalis fascia in type Ⅰ tympanoplasty. METHODS A retrospective analysis of 60 inflammatory chronic suppurative otitis media patients from January 2014 to January 2015 in Genenral Hospital of PLA Guangzhou Military Area were conducted.All cases were perpormed the ear incision under local anesthesia, including 32 cases(32 ears) with acellular dermal matrix, 28 cases(28 ears) with temporal fascia. The operation time, postoperative pain, the healing of tympanic membrane and the postoperative recovery were compared between the two groups. RESULTS There was significant difference in the operation time and postoperative pain between the two groups(P<0.05). There was no significant difference in the success rate of tympanic membrane healing and postoperative hearing improvement(P>0.05). CONCLUSION The effect of acellular dermal matrix for repairing tympanic membrane is similar to the temporalis fascia. The use of acellular dermal matrix has the advantage of shorter operation time, less postoperative pain, and can avoid regional two incisions, it is worthy of clinical application.

This article discusses the three-dimensional ambulance under the future war conditions.The unexpectedness of battlefields and complexity of the wounded in the future warfare necessitate the establishment of a three-dimensional system of medical rescue that can be organized by land,water and air.With an analysis of the features of modern war and based on the theory of modern treatment,the authors have proposed the requirements for designing the mode of three-dimensional rescue in the war zone and its typical procedures,set forth the view that rapid reaction and three-dimensional rescue are the most effective medical guarantee of combat strength,and provided a scientific basis for building a medical rescue system in the future warfare.

Objective To investigate the effects of Triptolide on apoptosis of cultured rat mesangial cells treated by TGF-β1 and the role of Smac in this process. Methods The mesangial cells were pre-treated with different concentrations of Triptolide for 24 hours, then stimulated with TGF-β1 for 24 hours. Apoptotic cells were detected by TUNEL assay. Smac transcription level was determined by Real time-PCR analyses. Smac expression level was assessed using Western blot anal-yses. Localization of Smac was shown by confocal fluorescence microscopy. Results Compared with control group, TGF-β1 inhibited apoptosis and Smac transcription and expression in rat mesangial cells. By contrast, Triptolide promoted mesangial cells apoptosis. In Triptolide groups, Smac mRNA and protein levels were up-regulated. Additionally, in normal and TGF-β1 groups Smac protein was mainly localized in mitochondriawhile in Triptolide groupit was mainly localized in cytoplasm and nucleus with increased fluorescence intensity. Conclusion Triptolide could promote the effect that TGF-β1 inhibited apop-tosis of mesangial cells, through both up-regulation the expression of Smac and stimulating it translocation from mitochon-dria to cytoplasm and nucleus.

Objective: To analyze the design of Nitinol Patellar Concentrator (NT-PC) and its biomechanic characteristics for treating comminuted patellar fractures.Methods:NT-PC and patellar was analyzed by ANSYS software,the finite element was three dimension brick unit with 20 nodes, 8 faces, and the degree of freedom was 6.In NT-PC,10 483 units were divided and the number of nodes was 17 959;In Patellar,8 775 units were divided and the number of nodes was 15 847.The patellar model was loaded according to the 5 converging directions of NT-PC.Results:NT-PC had tension force on its internal face and pressure force in its external face.The maximum value was 195.8 MPa and -18 MPa,which was far from its utmost stress and fatigue limit.The memory converging force of NT-PC was 134 N.The stress (0.8 MPa) was evenly distributed on the patellar surface.Stress calculation of every node indicated that positive stress was the main stress.Conclusion: The design of NT-PC is reasonable and has good reusing character.After fixation, exercise is allowed immediately after operation.The continual positive stress is good for the stability of fracture end and fracture healing.