Objective:To observe the effect and influence of homocysteine and cystatin C in type 2 diabetes patients treated by Shuxuening injection combined with gliclazide. Methods: Type 2 diabetic patients were randomly divided into observation group and control group,and there were 30 cases in each group. The control group was given gliclazide hypoglycemic treatment, on the basis of the observation group to combined with Shuxuening injection. The course of treatment was 2 weeks. Efficacy , adverse drug reactions ,Ho-mocysteine ( Hcy) , Cystatin C ( CysC) and blood glucose ( FBG, PB) level change of two groups patients before and after treatment were compared. Results:The total effective rate of the observation group was 96. 67%, which was significantly higher than that of the control group (P<0. 05). After treatment,blood glucose and Hcy, CysC levels of the two groups of patients were significantly de-creased compared with before treatment (P<0. 05),and those of the observation group was much lower(P<0. 05). The incidence of adverse drug reactions between the two groups had no significant difference(P>0. 05). Conclusion:Shuxuening Injection on patients with type 2 diabetes mellitus has good curative effect, it can effectively reduce homocysteine and cystatin C levels, better control of blood glucose.

OBJECTIVE: To observe the effects of glycyrrhizin on hydroxyproline(HYP),hyaluronic acid(HA) and laminin(LN) in pulmonary fibrosis model rats.METHODS: A total of 80 SD rats were randomly divided into normal control group,model group,hormone group(prednisone 0.6 mg?kg?d-1),and glycyrrhizin group(10 g?kg?d-1).The latter 3 groups were established into pulmonary fibrosis model with bleomycin,and on the second day after modelling,each group was given corresponding test drugs(the normal group and model group were treated with same amount of normal saline) then the changes of the levels of HYP in lung tissues,and serum levels of HA and LN in every group were observed at 7 and 28 days respectively.RESULTS: In glycyrrhizin group compared with model group,the contents of HYP in lung tissue,HA and LN in serum decreased significantly(P

OBJECTIVE: To explore the effect of glycyrrhizin on eotaxin expression in lung tissue from asthmatic model mice and the mechanism for glycyrrhizin to treat bronchial asthma.METHODS: The mice were randomized to asthma model group,prednisone-treated group and glycyrrhizin-treated group and normal control group.Egg protein asthma mouse model was established before being giving the corresponding drugs.Then the mice were sacrificed after 4 weeks for the counting of eosinophils in bronchoalveolar lavage fluid(BALF) and detection of Eotaxin expression in lung tissues by immunohistochemistry,with the results compared with normal control group.RESULTS: In glycyrrhizin-treated group compared with model group,the counting of eosinophils in BALF were decreased(P

BACKGROUND: Studies have shown that OCT4, SOX2 and HIWI can express in tumor tissues. OBJECTIVE: To investigate the relationship between the expression of stem cell transcription factors SOX2 and OCT4 in esophageal squamous cell carcinoma and their clinical characteristics and prognosis. METHODS: Expression levels of SOX2 and Oct4 were detected using immunohistochemical SP method in 77 cases of esophageal squamous cell carcinoma and 40 cases of adjacent cancer tissues confirmed by pathological examination, and then the their relationship with prognosis and clinical characteristics of the patients was analyzed. RESULTS AND CONCLUSION: Significantly higher levels of SOX2 and Oct4 were found in the esophageal cancer tissues than the adjacent cancer tissues (P < 0.05). SOX2 and Oct4 were highly expressed in the esophageal cancer tissues isolated from poorly differentiated, lymph node metastasis patients (P < 0.05). During the 2-year follow-up, the median survival of patients positive for SOX2 and Oct4 was only 24 and 21 months, respectively, which was significantly lower than that of negative patients (30 and 33 months, respectively). These findings indicate that the highly expressed SOX2 and Oct4 in esophageal cancer tissues are associated with the differentiation, metastasis and prognosis of the patients with esophageal squamous cell carcinoma.

This article analyzes and discusses histology's teaching mode and condition,to put forward a conceiving of altering classical modal to first experimental classes and then theoretical classes.The feasibility and expecting effect of new teaching model are concluded

y under uhrasonography is a more useful technique in diagnosing nonpalpable thyroid nodules.

This paper summarized and analyzed the reforming trends in ideas,methods and contents of morphology laboratory education in basic medical school.In order to fulfill the education goals of imparting knowledge and fostering capability and quality education,we should combine the traditional and virtual methods effectively,transform the secondary status ( verifying lecture) of morphology laboratory teaching to independent subject,try to integrate the subjects of morphology,use bilingual education and cultivate scientific research ability.

AIM: To explore the effect of liquorice on airway inflammation and Th_1/Th_2 imbalance in chronic asthma. METHODS: The chronic asthma model was established by intraperitoneal ovalbumin. The effect of liquorice on mice model of chronic bronchial asthma was observed, and the levels of serum IFN-? and IL-4 were tested by ELISA. RESULTS: Decreasing inflammatary cells infiltration in pulmonary tissue slices of tiny bronchial wall together with increasing serum IFN-? and decreasing serum IL-4 levels. CONCLUSION: Liquorice can adjust Th_1/Th_2 imbalance and inhibit the airway inflammation.

OBJECTIVETo study effects of polysaccharide of Radix Ranunculi Ternati (PRT) on immunological function and anti-oxidation activity of mouse.

METHODCell proliferations of splenocyte, thymocyte and peritoneal macrophage were measured by MTT colorimetry. The phagocytic function of peritoneal macrophage was measured by neutral red colorimetric method. The disoxidation power of PRT was measured by Prussian blue method. The clearing effect of PRT on hydroxyl radical was measured by salicylic acid capture method. The clearing effect of PRT on superoxide anion free radical was measured by pyrogallol auto oxidation method.

RESULTPRT among 25-400 mg x L(-1) could enhance thymocytes and spleen lymphocyte proliferation and macrophage phagocytosis. PRT(200 mg x L(-1)) has the strongest macrophage proliferation. PRT in different concentration has shown some disoxidation effects. PRT in 8 g x L(-1) has nearly the same ability of clearing x OH by Vit C with the same concentration. The clearance rate of PRT on O2*- is 95.39%.

CONCLUSIONPRT can enhance the cell proliferation capability of thymocytes, spleen lymphocytes and peritoneal macrophages. PRT can enhance macrophage phagocytosis in a dose-response relationship. PRT has saome disoxidation power and strong ability of clearing x OH and O2*-.

Animals ; Antioxidants ; analysis ; pharmacology ; Cell Proliferation ; drug effects ; Cells, Cultured ; Macrophages, Peritoneal ; cytology ; drug effects ; immunology ; Male ; Mice ; Oxidation-Reduction ; drug effects ; Phagocytosis ; drug effects ; Polysaccharides ; immunology ; pharmacology ; Ranunculus ; chemistry ; Spleen ; cytology ; drug effects ; immunology ; Thymus Gland ; cytology ; drug effects ; immunology

Objective To compare the effect of Acetate Sodium Potassium Magnesium Calcium and lactate ringers′ as priming solution in pediatrics with cardiopulmonary bypass (CPB) during congenital heart surgery. Methods Sixty children, aged 1-6 years, weighting 8 to 20 kg, with ASA Ⅱ to Ⅲ class and with ventricular or atrial septal defect elective , received heart surgery with CPB. They were randomly divided into two groups: the lactate ringers′ group (R) and the sodium acetate, potassium, magnesium and calcium glucose injection pre-filled group (L). Levels of artery blood gas, lactic, gluclose, electrolytes were detected at the time points of induction of anesthesia (T1), aortic cross damping (T2), stopping before (T3), and closing chest later (T4). Results Levels of the blood glucose and lactic acid in the two groups post-CPB were increased (P <0.01) at T2 and T3. Lactic acid level in group L was lower than that in group R (P < 0.05), and the blood glucose level in group L was higher than that in group R (P < 0.01). No significant difference was found in the lactic acid and blood glucose post-closing chestbetween the two groups (P > 0.05). Conclusion Sodium, potassium , magnesium , calcium and glucose injection as children CPB priming crystalloid fluid could provide energy substrate, reduce lactic acid concentration, with little effect on electrolyte and blood gas.