There are huge amount of TCM ancient books in libraries of TCM college, which are not only thousands of years of academic essence, but also are guidance for modern TCM theory and clinical practice. At the same time, they reflect Chinese traditional ideology and culture, and are significant to studying of traditional culture connotation. How to integrate ancient books resource and development resource service, which is key point of ancient books working in library. In this paper, some studying ideas are discussed from four aspects:Booklist recording objectively and information perfecting;Compilation of classified catalogue and construction of resource library;Introducing “Comprehensive ancient books” concept to promote integration and configuration of library resource;Investigation and comparison of relative edition for providing information service.

Objective To analyze the clinical characteristics of coronary artery bypass graft sur-gery for elderly patients over the age of 70 years. Methods The clinical data of 232 elderly patients ( age﹥70 years)who underwent coronary artery bypass graft surgery were collected for analysis,including 208 cases of simple coronary artery bypass graft surgery,11 cases of additional mitral valve replacement or annuloplasty,6 cases of additional aortic valve replacement,1 case of additional mitral and aortic valve re-placement,5 cases of additional mediastinal or pericardial tumor resection and 1 case of additional aneu-rysm resection. Results There were eight cases of postoperative respiratory failure,six cases of poor wound healing,4 cases of secondary exploration for postoperative bleeding,2 cases of perioperative myocar-dial infarction,1case of third-degree atrioventricular block and 1 cases of cardiac tamponade. Deaths oc-curred in 2 cases. Conclusion For elderly patients over the age of 70 years,overall effect of coronary ar-tery bypass graft surgery is satisfied. It can improve the quality of life and prolong survival time.

BACKGROUND: Mesenchymal stem cell transplantation for treatment of hypoxic-ischemic brain damage (HIBD) has obtained some outcomes in adult animals, but studies are few in neonatal animal models. Mesenchymal stem cells are commonly harvested from bone marrow. A few studies are on umbilical cord blood-mesenchymal stem cells (UCB-MSCs). OBJECTIVE: To investigate the feasibility and timeliness of UCB-MSC transplantation after injecting UCB-MSCs into neonatal rat models of HIBD. DESIGN, TIME AND SETTING: The complete randomized controlled animal experiment was performed at the Laboratory of Department of Neurology of First Hospital Affiliated to Nanchang University from October 2004 to July 2005. MATERIALS: A total of 38 healthy neonatal SD rats aged 7 days old were used to create rat models of HIBD. Three rats died. METHODS: Cord blood samples were collected after normal full-term delivery of 23-35 healthy pregnant women for culturing UCB-MSCs. MSCs were labeled with 4',6-diamidino-2-phenylindole 2hci (DAPI) in vitro before transplantation. Thirty-five rat models were divided into three groups. UCB-MSCs were injected into tail vein of twelve rat models in the early transplantation group two days after modeling. UCB-MSCs were injected into tail vein of twelve rat models in the late transplantation group one week after modeling. Same volume of saline was injected into eleven rats of the control group. Six rats from early transplantation and late transplantation groups each were respectively obtained at day 2 after transplantation and at week 2 after modeling. Three, four and four rats from control group were obtained respectively 2 days, 1 and 2 weeks after modeling, and sacrificed after anaesthesia. Ischemic brain tissues from the brain and hippocampal gyrum were sliced into frozen sections. MAIN OUTCOME MEASURES: Brain tissue pathomorphology was measured by Haematoxylin and Eosin Staining. Brain tissue DAPI-positive cells were detected with a fluorescence microscope. RESULTS: Brain edema at ischemic region, neural cell swelling and a decrease in cell number were tested in the control group. DAPI-positive UCB-MSCs were few in focal brain tissues, and swelling degree,extracellular space improvement and increased cell number were insignificant in the early transplantation group. One week after modeling, brain tissue extracellular space became small, cell number increased, and brain swelling reduced; A mass of DAPI-positive cells in rat focal brain migrated and diffused, without significant boundary in the late transplantation group. CONCLUSION: UCB-MSCs effectively traverse blood-brain barrier, and migrate, disperse and conform around focal brain tissues. A good outcome of transplantation is obtained at week 1 after HIBD.

OBJECTIVE:To provide reference for rational use of TCM injection in the clinic. METHODS:The utilization of TCM injections in 34 hospitals from Nanjing area during 2012-2014 was analyzed statistically in respects of consumption sum, DDDs,DDC. The occurrence of ADR induced by TCM injections was also analyzed statistically in the First Affiliated Hospital of Nanjing Medical University. RESULTS:The consumption sum of TCM injections in 34 hospitals from Nanjing area during 2012-2014 increased year by year;top 10 kinds of TCM injections mainly were anti-tumor drugs and cardio-cerebrovascular drugs in 2014,and each of them involved 4 categories and their consumption sum accounted for 46.08% and 32.59%,respectively. Total consumption sum of anti-tumor TCM injections increased slowly,while those of cardio-cerebrovascular drugs,antibiosis and anti-phlogosis increased rapidly. Lentinan injection steadily took up the top in the list of consumption sum and DDDs of single type,its DDC was the lowest and B/A=1.00. In the First Affiliated Hospital of Nanjing Medical University,most of ADR was induced by Xiyanping injection (27 cases,45.00%). CONCLUSIONS:The type of TCM injections keep stable in 34 hospitals from Nanjing area during 2012-2014,mainly involving anti-tumor drugs and cardio-cerebrovascular drugs which were used widely. Clinic should strengthen dialectial therapy close monitoring and attch improtance to the rational use of TCM injections.

By literature and network investigation,the paper sorts out development modes and typical practices of Internet medical treatment in China,sums up 6 kinds of modes existing domestically,analyzes the advantages and disadvantages of each mode.It is necessary to further probe into suitable operating modes and to give support on aspects of policies,laws and regulations and supervision,and provides reference for better promoting Internet medical treatment in different places.

Objective:To construct Cyanovirin-N(CV-N) expression plasmid pET-CVN and express the protein in E.coli, and to prepare specific antibody against Cyanovirin-N.Methods:In this study, the encoding sequence of Cyanovirin-N was obtained by PCR. The expression plasmid pET-CVN was constructed by inserting the encoding sequence of CV-N into pET-His containing T7 promoter and then BL21(DE3)strain was transformed. Expression of CV-N was induced by IPTG at 30℃ for 8 h. Cyanovirin-N was refolded by using dialysis method. The target protein was purified through Ni2+-NTA Agarose Fast Flow. The purified Cyanovirin-N was used as an antigen to immunize rats,and the polycolonal antibody to Cyanovirin-N was obtained.Results:Cyanovirin-N was cloned and expressed in E.coli successfully. SDS-PAGE analysis showed that the Cyanovirin-N was expressed in the form of insoluble inclusion body,and the expression amount of Cyanovirin-N was about 42.08% of total protein. With ELISA detection the titer of antibody was more than 1∶8 000, Western blot analysis showed that the antibody can bind with Cyanovirin-N specifically.Conclusion:Cyanovirin-N expression plasmid pET-CVN has been constructed, Cyanovirin-N with bioactivity has been obtained. The prepared antibody against Cyanovirin-N has well specificity and reactivity and can be used to detect the expression products by recombinant strains of E.coli.The work lays a foundation further study of its functions.

Objective To study the effect of electroacupuncture(EA) combined with transcranial magnetic stimulation(TMS) on the angiogenesis in rats with cerebral ischemia. Methods Fifty-five male Wistar rats were randomly divided into five groups: a normal group, a model group, an EA group, a TMS group and an EA plus TMS group. After the establishment of acute middle cerebral artery occlusion (MCAO), the rats in various groups were accordingly treated with EA, TMS or EA plus TMS, respectively. Then the expression of VEGF164 mRNA and CD31 was observed. Results The expression of VEGF164 mRNA and CD31 around the infarction locus of the three treatment groups increased, especially in the EA plus TMS group. Conclusion EA plus TMS can increase the expression of VEGF164 mRNA and CD31 around the infarction locus and promote the non-molecular angiogenesis in rats with cerebral ischemia.

Objective To explore the clinical value of glycosylated hemoglobin A1(HbA1c) ,C-reactive protein(CRP) ,interleu-kin-6(IL-6) and ischemia modified albumin(IMA) in patients with type 2 diabetes nephropathy .Methods 64 patients with type 2 diabetes mellitus were divided into simple diabetic mellitus group(34 patients) and diabetic nephropathy group(30 patients) .In ad-dition ,40 healthy subjects were chosen as control group .The levels of HbA1c ,CRP ,IL-6 and IMA were detected .Results The lev-els of HbA1c ,CRP ,IL-6 and IMA in simple diabetic mellitus group were all significantly higher than those in control group(P<0 .05) .The levels of HbA1c ,CRP ,IL-6 and IMA in diabetic nephropathy group were all significantly higher than those in simple di-abetic mellitus group(P<0 .05) .There were a positive correlations between the HbA1c level and the levels of CRP ,IL-6 ,IMA(P<0 .05) .The positive rate of combined detection of HbA1c ,CRP ,IL-6 and IMA was significantly higher than separate index detection (P<0 .05) .Conclusion The combined detection of HbA1c ,CRP ,IL-6 and IMA may be beneficial to the early diagnosis of diabetic nephropathy ,which will delay the progress of diabetic nephropathy .

Objective To explore the clinical effect of ligament advanced reinforcement system ( LARS) artificial ligament reconstruc-tion of the anterior cruciate ligament ( ACL) in the operation. Methods Sixty patients with ACL injury in our hospital from September 2011 to May 2013 were randomly divided into LARS group and autologous hamstring tendon ( group ST/G) group with 30 cases in each group,the Lysholm score,IKDC score,the difference of clinical effect and bone tunnel of patients in two groups before treatment and at 3rd,6th,9th, 18th months after treatment were compared. Results The time of walking with crutch,ambulation and restore movement in LARS group were obviously shorter than those in ST/G group(P<0. 05). The Lysholm score,IKDC score,the difference of clinical effect and bone tunnel of patients in two groups after treatment were better than those before treatment(P<0. 05). The difference of Lysholm score and IKDC score be-tween two groups before operation was not significant (P>0. 05). The Lysholm score and IKDC score of LARS group at 3rd,6th,9th months after operation was higher than that of ST/G group,and at 18th months after operation,the difference of Lysholm score and IKDC score be-tween two groups was not significant (P>0. 05). At the last follow-up,the distribution effect of LARS group was higher than that of ST/G group (P<0. 05). The excellent and good rate of LARS group (93. 33%) was higher than that in ST/G group(76. 67%),but the difference was not significant (P>0. 05). There were 6 cases of patients with bone tunnel enlargement in LARS group,14 patients had bone tunnel en-largement in ST/G group. The bone tunnel enlargement rate was significantly higher than that of the LARS group (P<0. 05). Conclusion Ligament advanced reinforcement system of artificial ligament in anterior cruciate ligament reconstruction with autologous hamstring tendon re-construction can significantly speed up the recovery of the patients,and improve the postoperative curative effect and reduce the incidence of postoperative bone tunnel enlargement.

Objective To observe the clinical effect of modified B-lynch suture in the treatment of patients with pernicious placenta pre-via.Methods A total of 46 patients with pernicious placenta previa in our hospital were randomly divided into the modified B-lynch suture treatment group(modified group)and the conventional therapy group(convention group),with 23 cases in each group.The operation time, menstrual recovery time and lochia sanguinea time,postpartum hemorrhage,intraoperative blood soss,abnormal temperature time,placenta ac-creta,hysterectomy and neonatal asphyxia between the two groups were compared.Results The intraoperative blood soss and postpartum hemorrhage of modified group was less than those of convention group,the menstrual recovery time was shorter than that of convention group and the rate of resection of uterine was lower than that of conventional group,with significant difference (P <0.05).There was no statistically significant difference in operation time,abnormal temperature time,lochia sanguinea time,placenta accreta and neonatal asphyxia between two groups (P >0.05).Conclusion The clinical effect of modified B-lynch suture in the treatment of dangerous placenta previa is significant.