The effect of colloidal bismuth subcitrate (CBS) in different doses on the rat gastric mucosal blood flow was measure by using laser dopper flow techinique. The results showed that CBS don't increase the gastric mucosal blood flow,but help to maintain a better blood flow in 20 mg,40 mg doses after the ethanol given,andCBS protected gastric mucosa of rat from ethanol-induced ulceration. This paper suggested that CBS may be decreased vascular permeability to protect the gastric mucosal blood from Ethanol damage.

Objective: To explore the relationships between Reinforcement sensitivity, Internet service hobby and Pathological internet use. Methods: 197 junior middle school students were administrated to complete Behavioral Inhibition System and Behavioral Activation Scale(Chinese edition), Adolescents Internet Service Hobby Questionnaire, and Adolescents Pathological Internet Use Scale. Results: ①For Internet service hobby and PIUs, the Social hobby and the Entertainment hobby wre somewhat correlated to PIUs positively; however, the Information hobby and the Bargains hobby were somewhat correlated to PIUs negatively, but not significantly; ②For Reinforcement sensitivity and PIUs, Path analysis suggests, high BAS individuals were easily involved in the PIU, but high BIS individuals were not. Conclusion: Reinforcement sensitivity is a significantly predictor of PIUs, but the Internet service hobby is not.

To determine the relationship between Barrett's esophagus (BE) and features of gastroesophageal acid reflux, 24 h esophageal pH monitoring was performed in 90 patients. The patients were divided into 3 groups: 31 subjects with BE, 21 with mild esophagitis and 38 with severe esophagitis. The following parameters were evaluated: the percentage time of pH < 4; the number of reflux episodes over 5 min; the duration of longest episodes and DeMeester score over total period and the auterior three parameters in erect and supine position. All these parameters in BE were significantly different from those with mild esophagitis (P < 0.01) and not significantly different from those with severe esophagitis (P > 0.05). During supine position all the above parameters in BE were significantly different from those with reflux esophagitis (P < 0.05). It is concluded that the quantity of acid reflux is not an important factor in development of BE in gastroesophageal reflux (GER), and the acid reflux in supine position might be important in development of BE in GER.
Barrett Esophagus/etiology ; Barrett Esophagus/*physiopathology ; Esophagitis, Peptic/etiology ; Gastroesophageal Reflux/*complications ; Hydrogen-Ion Concentration ; Supine Position

IrritabIe boweI syndrome( IBS)is caused by muItipIe factors,and the pathogenesis has not yet been cIarified. RecentIy,the roIe of brain-gut axis based bio-psycho-sociaI medicaI modeI in the pathogenesis of IBS has been wideIy accepted. Brain-gut axis forms a nerve-endocrine-immune network mediating bidirectionaI reguIation pathway,which pIays an important roIe in maintaining homeostasis between centraI nervous system and gut. Brain-gut axis abnormaIities may Iead to imbaIance of the homeostasis and subsequentIy induce the occurrence of IBS. This articIe reviewed the advances in study on brain-gut axis dysfunction in pathogenesis of IBS.

Irritable bowel syndrome(IBS)is one of the most common functional gastrointestinal disorders,which impacts on patients’quality of life as well as physical and mental health. Studies have shown that visceral hypersensitivity (VHS)is an important pathophysiological factor in the pathogenesis of IBS,and neural regulation plays a key role in the process of VHS. This article reviewed the advances in study on effect of neural regulation pathway on pathogenesis of VHS in IBS.

Objective To explore the value of asthma journal and corresponding monitoring of pulmonary ventilation function in treating bronchial asthma with the existing classification of asthma severity as control.Methods After 137 patients with bronchial asthma were evaluated according to the existing grades,they kept asthma diaries and received corresponding monitoring of pulmonary ventilation function at least 5 times per day,and all of the above clinical data were analyzed by relevant statistical methods.Results Among the 126 patients who completed monitoring,only 61(48.41%) patients could be graded by the Guide for Prevention and Treatment of Bronchial Asthma;27(21.43%) patients felt sensitive while 33(26.19%) ones felt insensitive.Conclusion The corresponding monitoring can not only accurately evaluate the severity of patients' bronchial asthma condition,but also detect those patients with deviated symptom perception.

Objective To investigate the relationship between the p27~(kip1) expression and the change of radiosensitivity of esophageal cancer cells.Methods Radio-resistant cells (EC9706R) were gradually isolated by means of repeated gamma-ray irradiation (6 000 cGy in total) upon human squamous esophageal carcinoma cells (EC9706).The radiosensitivity of these two types of cells was measured by standard colony formation assays,their cell cycle distribution analyzed by flow cytometry respectively,and the p27~(kip1) expression detected by immunocytochemistry.Results As compared with their parent cells,the isolated human squamous esophageal carcinoma cells(EC9706) showed clearly greater radio-resistance(for EC9706R,SF_2=65.71%,D_0=2.20 Gy,D_q=1.61 Gy,N=2.07,and for EC9706,SF_2=46.72％,D_0=1.61 Gy,D_q=0.99 Gy,N=1.85;SF_2,D_0,Dq and N were all higher).As to the cell cycle distribution,the population of G_1 phase cells in EC9706R cells was significantly decreased,but the proportion of S phase cells was significantly increased as compared with the parent cells.Immunocytochemistry revealed that the p27~(kip1) expression of EC9706R cells was clearly lower than that of EC9706 cells.Conclusion Cell phase change due to the decrease of p27~(kip1) expression may be one of the mechanisms of the generation of radio-resistance in esophageal cancer cells.

Objective To explore the role of intracranial electrode EEG monitoring for localizing epileptogenic zone in epilepsy.Methods 20 patients with refractory temporal lobe epilepsy,whose seizure onsets were unable to be localized by MRI、routine EEG and clinical characteristic,accepted long-term intracranial electrode EEG monitoring.Bitemporal lobe subdural implanted trip electrode were used to perform the ictal and interictal EEG monitoring under the guiding of the stereotactic technique.The results routine of EEG,MRI and intracranial electrode EEG were combined.The accuracy for localization of epilepitogenic zone was evaluated based on the followed-up.Results The intracranial electrode EEG monitoring was implanted within 1～5 d.Each patient was recorded the ictal and interictal discharge and was detected clinical onsets at least twice.All patients could be localized by ictal EEG.The consistency between interical EEG and ictal EEG were observated in 15 cases,the inconsistency in 3 cases,and 2 cases were bilateral discharge in interictal.The clinical outcome showed that patients were seizure-free in 13 cases(65%),improved significantly in 3 cases(15%),improved in 3 cases(15%),unimproved in 1 case(5%)according to Engel's criteria after surgery.No significant complication was found in all patients.Conclusion The intracranial electrode EEG monitoring can localize epileptogenic zone and offer reliable evidence for epilepsy surgery.

Objective To explore the early diagnostic value of quantitative sensory testing (QST) in diabetes with peripheral neuropathy (PNP).Methods The nerve conduction velocity (NCV) and QST were examined in 46 diabetic patients, and their results were compared. Results The abnormal ratio of NCV in 46 patients was 72.8%. 32 cases (69.6%) were diagnosed as diabetes with PNP. While using QST, the abnormal ratio was 91.3%. 40 cases (86.9%) were diagnosed as diabetes with PNP. There was a significant difference between two methods ( P

Objective Non steroidal anti inflammatory drugs(NSAIDs)can induce apoptosis in gastric cancer cell and the mechanism is not clear. We aimed to study the mechanism of selective COX 2 inhibitor Nimesulide induced apoptosis of human gastric cancer line SGC 7901 by detecting the expressions of COX 2 at mRNA level, c myc, Bcl 2 and caspase 3 at protein level. Methods Apoptosis was determined by electronic microscopy, Annexin V FITC staining and flow cytometry. The mRNA of COX 2 was detected by RT PCR. The protein expressions of c myc, Bcl 2 and caspase 3 were examined by immunohistochemistry. Results Nimesulide of 50 ?mol/L at 48 and 72 h, and of 100 ?mol/L and 200 ?mol/L at 24, 48 and 72 h induced apoptosis of gastric cancer cells in a dose and time dependent manner.Their apoptotic rates were 7.51%, 9.86% and 11.58%, 12.45%, 16.66% and 12.21%, 15.38%, 20.28% respectively. It increased c myc and caspase 3 expression and decreased Bcl 2 expression and COX 2 mRNA expression. The positive protein expression rates of Bcl 2, c myc and capase 3 were (20.2?7.6)%,(49.2?15.1)% and (34.6?12.9)% respectively with Nimesulide of 200 ?mol/L at 72 h,while the controls being (44.6?12.1)%, (24.7 ?9.5)% and (14.8?6.4)% the three comparative P